Dehydrodolichyl Diphosphate Synthase (DHDDS) (NM_001243565) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CIT; CPT; DEDSM; DS; hCIT; HDS; RP59
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330147 representing NM_001243565. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTCATGGATCAAGGAAGGAGAGCTGTCACTTTGGGAGCGGTTCTGTGCCAACATCATAAAGGCAGGC CCAATGCCGAAACACATTGCATTCATAATGGACGGGAACCGTCGCTATGCCAAGAAGTGCCAGGTGGAG CGGCAGGAAGGCCACTCACAGGGCTTCAACAAGCTAGCTGAGACTCTGCGGTGGTGTTTGAACCTGGGC ATCCTAGAGGTGACAGTCTACGCATTCAGCATTGAGAACTTCAAACGCTCCAAGAGTGAGGTAGACGGG CTTATGGATCTGGCCCGGCAGAAGTTCAGCCGCTTGATGGAAGAAAAGTGTTTCCTGAATGTCTGTTTT GCATACACATCCCGTCATGAGATCAGCAATGCTGTGAGAGAGATGGCCTGGGGGGTGGAGCAAGGCCTG TTGGATCCCAGTGATATCTCTGAGTCTCTGCTTGATAAGTGCCTCTATACCAACCGCTCTCCTCATCCT GACATCTTGATACGGACTTCTGGAGAAGTGCGGCTGAGTGACTTCTTGCTATGGCAGACCTCTCACTCC TGCCTGGTGTTCCAACCCGTTCTGTGGCCAGAGTATACATTTTGGAACCTCTTCGAGGCCATCCTGCAG TTCCAGATGAACCATAGCGTGCTTCAGAAGGCCCGAGACATGTATGCAGAGGAGCGGAAGAGGCAGCAG CTGGAGAGGGACCAGGCTACAGTGACAGAGCAGCTGCTGCGAGAGGGGCTCCAAGCCAGTGGGGACGCC CAGCTCCGAAGGACACGCTTGCACAAACTCTCGGCCAGACGGGAAGAGCGAGTCCAAGGCTTCCTGCAG GCCTTGGAACTCAAGCGAGCTGACTGGCTGGCCCGTCTGGGCACTGCATCAGCCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001243565
Insert Size	885 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001243565.1
RefSeq Size	3223 bp
RefSeq ORF	885 bp
Locus ID	79947
UniProt ID	Q86SQ9
Protein Pathways	Terpenoid backbone biosynthesis
MW	34.3 kDa
Gene Summary	The protein encoded by this gene catalyzes cis-prenyl chain elongation to produce the polyprenyl backbone of dolichol, a glycosyl carrier lipid required for the biosynthesis of several classes of glycoproteins. Mutations in this gene are associated with retinitis pigmentosa type 59. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011] Transcript Variant: This variant (4) lacks an in-frame coding exon compared to variant 1. This results in a shorter isoform (4) missing an internal protein segment compared to isoform 1.

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