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SARS2 (NM_001145901) Human Untagged Clone
CAT＃: SC327060
SARS2 (untagged)-Human seryl-tRNA synthetase 2 mitochondrial (SARS2) nuclear gene encoding mitochondrial protein transcript variant 1
CNY 8,360.00
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热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | mtSerRS; SARS; SARSM; SerRS; SerRSmt; SERS; SYS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC327060 representing NM_001145901.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCTGCGTCCATGGCGCGGCGCTTGTGGCCTTTGCTGACTCGTCGGGGGTTCCGGCCCCGGGGAGGC TGCATCTCCAACGATAGTCCAAGGAGAAGTTTCACTACAGAGAAACGAAACCGGAACCTCCTGTACGAG TATGCGCGCGAGGGCTACAGCGCACTCCCTCAGCTGGACATAGAGCGGTTCTGCGCATGCCCAGAAGAG GCCGCACACGCCCTGGAGCTCCGCAAGGGGGAGCTGCGCTCGGCGGACCTGCCCGCGATCATCTCGACA TGGCAGGAGCTGAGGCAGCTGCAGGAGCAGATCCGGAGCCTGGAGGAAGAGAAGGCAGCTGTGACTGAG GCAGTGCGGGCCCTGCTGGCAAACCAGGACAGTGGTGAAGTGCAGCAGGTGCGGCTGGATCCAGGTGCT GGCTCCATATTTGGTCCTACGTTCCTCCCATTCCCAGGCCAGCTTTCTCTCCTTGTGGAGGCCCAGCTT GAGGAGCAGTTCTACCTGCAGGCGCTGAAGCTGCCCAACCAGACCCACCCAGACGTGCCCGTCGGGGAT GAGAGCCAGGCTCGAGTGCTCCACATGGTCGGAGACAAGCCAGTTTTCTCCTTCCAACCTCGGGGCCAC CTGGAAATTGGCGAGAAACTCGACATCATCCGTCAGAAGCGCCTGTCCCACGTGTCTGGCCACCGGTCC TATTACCTGCGCGGGGCTGGAGCCCTCCTGCAGCACGGCCTGGTCAACTTCACATTCAACAAGCTTCTC CGCCGGGGCTTCACCCCCATGACGGTGCCAGACCTTCTCCGCGGAGCAGTGTTTGAAGGCTGTGGGATG ACACCAAATGCCAACCCATCCCAAATTTACAACATCGACCCTGCCCGCTTCAAAGATCTCAACCTGGCT GGAACAGCGGAGGTGGGGCTTGCAGGCTACTTCATGGACCACACCGTGGCCTTCAGGGACCTGCCAGTC AGGATGGTTTGCTCCAGCACCTGCTACCGGGCAGAGACAAACACGGGACAGGAACCCCGGGGGCTGTAT CGAGTACACCACTTCACCAAGGTGGAGATGTTTGGGGTGACAGGCCCTGGGCTGGAGCAGAGCTCACAG CTGCTGGAGGAGTTCCTGTCCCTTCAGATGGAGATCTTGACAGAGCTGGGCTTGCACTTCCGGGTCCTG GATATGCCCACCCAAGAACTGGGCCTCCCCGCCTACCGCAAGTTTGACATTGAGGCCTGGATGCCAGGC CGAGGCCGCTTTGGAGAGGTCACCAGTGCTTCCAACTGCACAGACTTCCAGAGCCGCCGCCTCCACATC ATGTTCCAGACCGAGGCTGGGGAGCTGCAGTTTGCCCACACGGTGAACGCCACCGCCTGTGCTGTCCCC CGCCTTCTCATCGCGCTCCTGGAGAGTAACCAGCAGAAGGACGGCTCAGTGCTCGTGCCCCCTGCCCTC CAGTCCTACCTCGGCACTGATCGGATCACAGCCCCTACCCACGTGCCTCTCCAGTACATCGGCCCCAAC CAGCCCCGGAAGCCTGGGCTGCCTGGCCAGCCTGCTGTAAGCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001145901 |
| Insert Size | 1563 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001145901.1 |
| RefSeq Size | 2083 bp |
| RefSeq ORF | 1563 bp |
| Locus ID | 54938 |
| UniProt ID | Q9NP81 |
| Protein Pathways | Aminoacyl-tRNA biosynthesis |
| MW | 58 kDa |
| Gene Summary | This gene encodes the mitochondrial seryl-tRNA synthethase precursor, a member of the class II tRNA synthetase family. The mature enzyme catalyzes the ligation of Serine to tRNA(Ser) and participates in the biosynthesis of selenocysteinyl-tRNA(sec) in mitochondria. The enzyme contains an N-terminal tRNA binding domain and a core catalytic domain. It functions in a homodimeric form, which is stabilized by tRNA binding. This gene is regulated by a bidirectional promoter that also controls the expression of mitochondrial ribosomal protein S12. Both genes are within the critical interval for the autosomal dominant deafness locus DFNA4 and might be linked to this disease. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Mar 2009] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). |
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| RG228425 | SARS2 (tGFP-tagged) - Human seryl-tRNA synthetase 2, mitochondrial (SARS2), nuclear gene encoding mitochondrial protein, transcript variant 1 |
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