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Bestrophin (BEST1) (NM_001139443) Human Untagged Clone
CAT＃: SC325224
BEST1 (untagged)-Human bestrophin 1 (BEST1), transcript variant 2
CNY 4,504.00
CNY 7,220.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ARB; BEST; Best1V1Delta2; BMD; RP50; TU15B; VMD2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_001139443 edited
CACCAGCCTAGTCGCCAGACCTTCTGTGGGATCATCGGACCCACCTGGAACCCCACCTGC TGGCCCTCACGGAAGAACAACAGCTGATGTTTGAGAAACTGACTCTGTATTGCGACAGCT ACATCCAGCTCATCCCCATTTCCTTCGTGCTGGGCTTCTACGTGACGCTGGTCGTGACCC GCTGGTGGAACCAGTACGAGAACCTGCCGTGGCCCGACCGCCTCATGAGCCTGGTGTCGG GCTTCGTCGAAGGCAAGGACGAGCAAGGCCGGCTGCTGCGGCGCACGCTCATCCGCTACG CCAACCTGGGCAACGTGCTCATCCTGCGCAGCGTCAGCACCGCAGTCTACAAGCGCTTCC CCAGCGCCCAGCACCTGGTGCAAGCAGGCTTTATGACTCCGGCAGAACACAAGCAGTTGG AGAAACTGAGCCTACCACACAACATGTTCTGGGTGCCCTGGGTGTGGTTTGCCAACCTGT CAATGAAGGCGTGGCTTGGAGGTCGAATCCGGGACCCTATCCTGCTCCAGAGCCTGCTGA ACGAGATGAACACCTTGCGTACTCAGTGTGGACACCTGTATGCCTACGACTGGATTAGTA TCCCACTGGTGTATACACAGGTGGTGACTGTGGCGGTGTACAGCTTCTTCCTGACTTGTC TAGTTGGGCGGCAGTTTCTGAACCCAGCCAAGGCCTACCCTGGCCATGAGCTGGACCTCG TTGTGCCCGTCTTCACGTTCCTGCAGTTCTTCTTCTATGTTGGCTGGCTGAAGGTGGCAG AGCAGCTCATCAACCCCTTTGGAGAGGATGATGATGATTTTGAGACCAACTGGATTGTCG ACAGGAATTTGCAGGTGTCCCTGTTGGCTGTGGATGAGATGCACCAGGACCTGCCTCGGA TGGAGCCGGACATGTACTGGAATAAGCCCGAGCCACAGCCCCCCTACACAGCTGCTTCCG CCCAGTTCCGTCGAGTCTCCTTTATGGGCTCCACCTTCAACATCAGCCTGAACAAAGAGG AGATGGAGTTCCAGCCCAATCAGGAGGACGAGGAGGATGCTCACGCTGGCATCATTGGCC GCTTCCTAGGCCTGCAGTCCCATGATCACCATCCTCCCAGGGCAAACTCAAGGACCAAAC TACTGTGGCCCAAGAGGGAATCCCTTCTCCACGAGGGCCTGCCCAAAAACCACAAGGCAG CCAAACAGAACGTTAGGGGCCAGGAAGACAACAAGGCCTGGAAGCTTAAGGCTGTGGACG CCTTCAAGTCTGCCCCACTGTATCAGAGGCCAGGCTACTACAGTGCCCCACAGACGCCCC TCAGCCCCACTCCCATGTTCTTCCCCCTAGAACCATCAGCGCCGTCAAAGCTTCACAGTG TCACAGGCATAGACACCAAAGACAAAAGCTTAAAGACTGTGAGTTCTGGGGCCAAAAAAA GTTTTGAATTGCTCTCAGAGAGCGATGGGGCCTTGATGGAGCACCCAGAAGTATCTCAAG TGAGGAGGAAAACTGTGGAGTTTAACCTGACGGATATGCCAGAGATCCCCGAAAATCACC TCAAAGAACCTTTGGAACAATCACCAACCAACATACACACTACACTCAAAGATCACATGG ATCCTTATTGGGCCTTGGAAAACAGGTCTGTCCTCCACCTGAACCAGGGGCACTGCATTG CCCTGTGCCCCACCCCAGCTTCCCTTGCTCTGAGCCTACCCTTCCTCCACAATTTCCTAG GGTTCCATCACTGCCAGAGCACACTGGACCTACGCCCAGCACTGGCTTGGGGTATATACT TGGCCACCTTCACAGGGATCCTAGGGAAGTGTTCGGGACCTTTTCTCACTTCACCCTGGT ATCACCCGGAAGACTTCTTGGGACCAGGTGAAGGAAGATGAGGTTGTGCTGACCAGAATG CTGCTGGAGAACTGCCCCAGGGCTGACAGGCCAGGCTTAGCTGAGCAGATGTTATCACTG GCCCCAACTTACTTTGAGCAAGGGTGGCTGACCCAAAACCATGAGGTGGCAGTCAGCTGG ATGACAGATGAACACTTCCCCCATAACTATTTAGGGTAGTACCCAAGCACTACAGGAAAG GGTGGCAGGAACTGCCTCACTCCTAGGAACTGGTAGATGGTGAGGTTGAGGGTGTCCAGC GCCCTTAGGTCATTTTCTCACTGCCTGGGAACCTCACCAAAATACTTCTTGCTTCCTTGG GGTCAGCCCAAAGCTGTCACAAAATCAGATATTTCCCTTTATTCCAGATTTCCTGGACAC TTTCACCCAATTATAAACACCCCACTTCAGCCCCAATCACGTGGGAGGAAGTGTAACTTC CCTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_001139443 |
| Insert Size | 2400 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001139443.1, NP_001132915.1 |
| RefSeq Size | 2852 bp |
| RefSeq ORF | 1815 bp |
| Locus ID | 7439 |
| UniProt ID | O76090 |
| Protein Families | Druggable Genome, Ion Channels: Other, Transmembrane |
| Gene Summary | This gene encodes a member of the bestrophin gene family. This small gene family is characterized by proteins with a highly conserved N-terminus with four to six transmembrane domains. Bestrophins may form chloride ion channels or may regulate voltage-gated L-type calcium-ion channels. Bestrophins are generally believed to form calcium-activated chloride-ion channels in epithelial cells but they have also been shown to be highly permeable to bicarbonate ion transport in retinal tissue. Mutations in this gene are responsible for juvenile-onset vitelliform macular dystrophy (VMD2), also known as Best macular dystrophy, in addition to adult-onset vitelliform macular dystrophy (AVMD) and other retinopathies. Alternative splicing results in multiple variants encoding distinct isoforms.[provided by RefSeq, Nov 2008] Transcript Variant: This variant (2) encodes the longest isoform (2). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Effects of alternative splicing on function of Bestrophin-1 calcium-activated chloride channels
,Kuo, YH;Abdullaev, IF;Hyzinski-Garcia, MC;Mongin, AA;,
Biochem. J. Dec 2013.
,PubMed ID 24341532
[BEST1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227442 | BEST1 (Myc-DDK-tagged)-Human bestrophin 1 (BEST1), transcript variant 2 |
CNY 4,496.00 |
|
| RC227442L1 | Lenti ORF clone of Human bestrophin 1 (BEST1), transcript variant 2, Myc-DDK-tagged |
CNY 6,896.00 |
|
| RC227442L2 | Lenti ORF clone of Human bestrophin 1 (BEST1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC227442L3 | Lenti ORF clone of Human bestrophin 1 (BEST1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC227442L4 | Lenti ORF clone of Human bestrophin 1 (BEST1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG227442 | BEST1 (tGFP-tagged) - Human bestrophin 1 (BEST1), transcript variant 2 |
CNY 6,096.00 |
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