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RYK (NM_002958) Human Untagged Clone
CAT＃: SC323660
RYK (untagged)-Kinase deficient mutant (K234M) of Human RYK receptor-like tyrosine kinase (RYK), transcript variant 2
CNY 4,528.00
CNY 9,790.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | D3S3195; JTK5; JTK5A; RYK1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_002958, the custom clone sequence may differ by one or more nucleotides
ATGCGTGGGGCGGCGCGGCTGGGGCGGCCGGGCCGGAGTTGCCTCCCGGGGGCCCGCGGCCTGAGGGCCC CGCCGCCGCCGCCGCTGCTGCTTCTGCTTGCGCTGTTGCCGCTGCTGCCCGCGCCTGGCGCTGCCGCCGC CCCCGCCCCGCGGCCCCCGGAGCTGCAGTCGGCTTCCGCGGGGCCCAGCGTGAGTCTCTACCTGAGCGAG GACGAGGTGCGCCGGCTGATCGGTCTTGATGCAGAACTTTATTATGTGAGAAATGACCTTATTAGTCACT ACGCTCTATCCTTTAGTCTGTTAGTACCCAGTGAGACAAATTTCCTGCACTTCACCTGGCATGCGAAGTC CAAGGTTGAATATAAGCTGGGATTCCAAGTGGACAATGTTTTGGCAATGGATATGCCCCAGGTCAACATT TCTGTTCAGGGGGAAGTTCCACGCACTTTATCAGTGTTTCGGGTAGAGCTTTCCTGTACTGGCAAAGTAG ATTCTGAAGTTATGATACTAATGCAGCTCAACTTGACAGTAAATTCTTCAAAAAATTTTACCGTCTTAAA TTTTAAACGAAGGAAAATGTGCTACAAAAAACTTGAAGAAGTAAAAACTTCAGCCTTGGACAAAAACACT AGCAGAACTATTTATGATCCTGTACATGCAGCTCCAACCACTTCTACGCGTGTGTTTTATATTAGTGTAG GGGTTTGTTGTGCAGTAATATTTCTCGTAGCAATAATATTAGCTGTTTTGCACCTTCATAGTATGAAAAG GATTGAACTGGATGACAGCATTAGTGCCAGCAGTAGTTCCCAAGGGCTGTCTCAGCCATCCACCCAGACG ACTCAGTATCTGAGAGCAGACACGCCCAACAATGCAACTCCTATCACCAGTTATCCTACCTTGCGGATAG AGAAGAACGACTTGAGAAGTGTCACTCTTTTGGAGGCCAAAGGCAAGGTGAAGGATATAGCAATATCCAG AGAGAGGATAACTCTAAAAGATGTACTCCAAGAAGGTACTTTTGGGCGTATTTTCCATGGGATTTTAATA GATGAAAAAGATCCAAATAAAGAAAAACAAGCATTTGTCAAAACAGTTAAAGATCAAGCTTCTGAAATTC AGGTGACAATGATGCTCACTGAAAGTTGTAAGCTGCGAGGTCTTCATCACAGAAATCTTCTTCCTATTAC TCATGTGTGTATAGAAGAAGGAGAAAAGCCCATGGTGATATTGCCTTACATGAATTGGGGGAATCTTAAA TTGTTTTTACGACAGTGCAAGTTAGTAGAGGCCAATAATCCACAGGCAATTTCTCAGCAAGACCTGGTAC ACATGGCTATTCAGATTGCCTGTGGAATGAGCTACCTGGCCAGAAGGGAAGTCATCCACAAAGACCTGGC TGCCAGGAACTGTGTCATTGATGACACACTTCAAGTTAAGATCACAGACAATGCCCTCTCCAGAGACTTG TTCCCCATGGACTATCACTGTCTGGGGGACAATGAAAACAGGCCAGTTCGTTGGATGGCTCTTGAAAGTC TGGTTAATAACGAGTTCTCTAGCGCTAGTGATGTGTGGGCCTTTGGAGTGACGCTGTGGGAACTCATGAC TCTGGGCCAGACTCCCTACGTGGACATTGACCCCTTCGAGATGGCCGCATACCTGAAAGATGGTTACCGA ATAGCCCAGCCAATCAACTGTCCTGATGAATTATTTGCTGTGATGGCCTGTTGCTGGGCCTTAGATCCAG AGGAGAGGCCCAAGTTTCAGCAGCTGGTACAGTGCCTAACAGAGTTTCATGCAGCCCTGGGGGCCTACGT CTGA >OriGene 5' read for mutant NM_002958 unedited
CCCGCCGTTTGAGCAATGGGCGGTAGGCGTGTACGGTTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTG AACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGCGTCCCGCG GGCAGCCCCCAACTCCGGACGATGCAGCGAGGAGGCGGCGGCGGCGGCGCGCTCCGGAGGTCTGAAGCCA CTGCTGGCTGCCACGGGCCGGCGGTGCGATGAGCTGGGCAGCCGCCCCCGCCCCGCGGCCCCCGGAGCTG CAGTCGGCTTCCGCGGGGCCCAGCGTGAGTCTCTACCTGAGCGAGGACGAGTGCGCCGCTGATCGTCTTG ATGCAGACTTTATTATGTGAAAAATGACCTTATTAATTCACTAACGCCTCCTATCCCTTTATTCTTGGTT ATTACCCATTGAACACAAATTTCCCTGCACCTTCCACCTTGGCATGCCCGAAGTCCCAGGGTGGGAATAA TAGGCCTTGGAAATTCCAGGGTGGGACAAGGTTTTTGCCAAGGAGATATGCCCCCAGGTCACACATTTTT GGTTTCGGGGGGAAATTTCCCCCCCCTTTTTTAGTGTGTTTCGGGGAAACGTTTTCCGGGGCGGGGCAAG TTATATTTGTAAGTTTTGAAATCAAAGGGCCCCCTAACTTTGGCGGGAAATTTTTCTCAAAAATTTTTTC CCCTTTTAATTTTTTTAAACAAAGGGGAAAGGGGGCTAAAAAAAATTTTGAAAAATATTAAAACTCTTTC CCCTGTGGGAAAAAAACCCCTCACAAAACACCAATAAAAAACCGGACAACAGGCGCCCCCCCCTTTTTCT AGCCGCGGGGTAAAAAAAAGGCGGGGGGGTCTGGGTCCACAAATTTTTTCTCACACCAATTATTATCTGT TTTGCCCCCCACATATAGAGAGAATATACTGTCGAGACAGG >SC323660 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
GMTCCAGATGTACTTTTGGGCGTATTTTCCATGGGATTTTAATAGATGAAAAAGATCCAAATAAAGAAAA ACAAGCATTTGTCATGACAGTTAAAGATCAAGCTTCTGAAATTCAGGTGACAATGATGCTCACTGAAAGT TGTAAGCTGCGAGGTCTTCATCACAGAAATCTTCTTCCTATTACTCATGTGTGTATAGAAGAAGGAGAAA AGCCCATGGTGATATTGCCTTACATGAATTGGGGGAATCTTAAAT |
| Restriction Sites | Please inquire |
| ACCN | NM_002958 |
| Insert Size | 3100 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002958.2, NP_002949.2 |
| RefSeq Size | 2951 bp |
| RefSeq ORF | 1824 bp |
| Locus ID | 6259 |
| UniProt ID | P34925 |
| Domains | pkinase, TyrKc, S_TKc, WIF |
| Protein Families | Druggable Genome, Protein Kinase, Transmembrane |
| Gene Summary | The protein encoded by this gene is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. The encoded protein has a leucine-rich extracellular domain with a WIF-type Wnt binding region, a single transmembrane domain, and an intracellular tyrosine kinase domain. This protein is involved in stimulating Wnt signaling pathways such as the regulation of axon pathfinding. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1, resulting in a shorter protein (isoform 2). |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215509 | RYK (Myc-DDK-tagged)-Human RYK receptor-like tyrosine kinase (RYK), transcript variant 2 |
CNY 4,520.00 |
|
| RC215509L1 | Lenti ORF clone of Human RYK receptor-like tyrosine kinase (RYK), transcript variant 2, Myc-DDK-tagged |
CNY 6,920.00 |
|
| RC215509L2 | Lenti ORF clone of Human RYK receptor-like tyrosine kinase (RYK), transcript variant 2, mGFP tagged |
CNY 7,220.00 |
|
| RC215509L3 | Lenti ORF clone of Human RYK receptor-like tyrosine kinase (RYK), transcript variant 2, Myc-DDK-tagged |
CNY 7,220.00 |
|
| RC215509L4 | Lenti ORF clone of Human RYK receptor-like tyrosine kinase (RYK), transcript variant 2, mGFP tagged |
CNY 7,220.00 |
|
| RG215509 | RYK (tGFP-tagged) - Human RYK receptor-like tyrosine kinase (RYK), transcript variant 2 |
CNY 5,890.00 |
|
| SC310220 | RYK (untagged)-Human RYK receptor-like tyrosine kinase (RYK), transcript variant 2 |
CNY 4,528.00 |
