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ALPK1 (NM_025144) Human Untagged Clone
CAT＃: SC323654
ALPK1 (untagged)-Kinase deficient mutant (K1067M) of Human alpha-kinase 1 (ALPK1), transcript variant 1
CNY 8,672.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | 8430410J10Rik; LAK |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323654 sequence for NM_025144 edited (data generated by NextGen Sequencing)
ATGAATAATCAAAAAGTGGTAGCTGTGCTACTGCAAGAGTGCAAGCAAGTGCTGGATCAG CTCTTGTTGGAAGCGCCAGATGTGTCGGAAGAGGACAAGAGCGAGGACCAGCGCTGCAGA GCTTTACTCCCCAGCGAGTTAAGGACCCTGATCCAGGAGGCAAAGGAAATGAAGTGGCCC TTCGTGCCTGAAAAGTGGCAGTACAAACAAGCCGTGGGCCCAGAGGACAAAACAAACCTG AAGGATGTGATTGGCGCCGGGTTGCAGCAGTTACTGGCGTCCCTGAGGGCCTCCATCCTC GCTCGGGACTGTGCGGCTGCGGCGGCTATTGTGTTCTTGGTGGACCGGTTCCTGTATGGG CTCGACGTCTCTGGAAAACTTCTGCAGGTCGCCAAAGGTCTCCACAAGTTGCAGCCAGCC ACGCCAATTGCCCCGCAGGTGGTTATTCGCCAAGCCCGAATCTCCGTGAACTCAGGAAAA CTTTTAAAAGCAGAGTATATTCTGAGCAGTCTAATAAGCAACAATGGAGCAACGGGTACC TGGCTGTACAGAAATGAAAGTGACAAGGTCCTGGTGCAGTCGGTCTGTATACAGATCAGA GGGCAGATTCTGCAAAAGCTGGGGATGTGGTACGAAGCAGCAGAGTTAATATGGGCCTCC ATTGTAGGATATTTGGCACTTCCTCAGCCGGATAAAAAGGGCCTCTCCACGTCGCTAGGT ATACTGGCAGACATCTTTGTTTCCATGAGCAAGAACGATTATGAAAAGTTTAAAAACAAT CCACAAATTAATTTGAGCCTGCTGAAGGAGTTTGACCACCATTTGCTGTCCGCTGCAGAA GCCTGCAAGCTGGCAGCTGCCTTCAGTGCCTATACGCCGCTCTTCGTGCTCACAGCTGTG AATATCCGTGGCACGTGTTTATTGTCCTACAGTAGTTCAAATGACTGTCCTCCAGAATTG AAAAACTTACATCTGTGTGAAGCCAAAGAGGCCTTTGAGATTGGCCTCCTCACCAAGAGA GATGATGAGCCTGTTACTGGAAAACAGGAGCTTCACAGCTTTGTCAAAGCTGCTTTCGGT CTCACCACAGTGCACAGAAGGCTCCATGGGGAGACAGGGACGGTCCATGCAGCAAGTCAG CTCTGTAAGGAAGCAATGGGGAAGCTGTACAATTTCAGCACTTCCTCCAGAAGTCAGGAC AGAGAAGCTCTGTCTCAAGAAGTTATGTCTGTGATTGCCCAGGTGAAGGAACATTTACAA GTTCAAAGCTTCTCAAATGTAGATGACAGATCTTATGTTCCCGAGAGTTTCGAGTGCAGG TTGGATAAACTTATCTTGCATGGGCAAGGGGATTTCCAAAAAATCCTTGACACCTATTCA CAGCACCATACTTCGGTGTGTGAAGTATTTGAAAGTGATTGTGGAAACAACAAAAATGAA CAGAAAGATGCAAAAACAGGAGTCTGCATCACTGCTCTAAAAACAGAAATAAAAAACATA GATACTGTGAGTACTACTCAAGAAAAGCCACATTGTCAAAGAGACACAGGAATATCTTCC TCCCTAATGGGTAAGAATGTTCAGAGGGAACTCAGAAGGGGAGGAAGGAGAAACTGGACC CATTCTGATGCATTTCGAGTCTCCTTGGATCAAGATGTGGAGACTGAGACTGAGCCATCG GACTACAGCAATGATGAGGGAGCTGTTTTCAACAAGTCTCTGAGTGGCAGCCAGACTTCC AGTGCTTGGAGCAACTTATCAGGGTTTAGTTCCTCTGCAAGCTGGGAGGAAGTGAATTAT CACGTTGACGACAGGTCAGCCAGAAAAGAGCCTGGCAAAGAACATCTGGTGGACACTCAG TGTTCCACTGCCTTGTCTGAGGAGCTAGAGAATGACAGGGAAGGCAGAGCTATGCATTCA TTGCGTTCACAGCTTCATGATCTCTCTCTTCAGGAACCCAACAATGACAATTTGGAGCCT TCTCAAAATCAGCCACAGCAACAGATGCCCTTGACACCCTTCTCGCCTCATAATACCCCA GGCATTTTCTTGGCCCCTGGTGCAGGGCTTCTAGAAGGAGCTCCAGAAGGTATCCAGGAA GTCAGAAACATGGGACCCAGAAATACTTCTGCTCACTCCAGACCCTCATATCGTTCTGCT TCTTGGTCTTCTGATTCTGGTAGGCCCAAGAATATAGGCACACATCCTTCAGTCCAAAAA GAAGAAGCCTTTGAAATAATTGTTGAGTTTCCAGAAACCAACTGCGATGTCAAAGACAGG CAGGGGAAAGAGCAGGGAGAAGAAATTAGTGAAAGAGGCGCAGGCCCTACATTTAAAGCT AGTCCCTCCTGGGTTGACCCAGAAGGAGAAACAGCAGAAAGCACTGAAGATGCACCCTTA GACTTTCACAGGGTCCTGCACAATTCTCTGGGAAACATTTCCATGCTGCCATGTAGCTCC TTCACCCCTAATTGGCCTGTTCAAAATCCTGACTCCAGAAAAAGTGGTGGCCCAGTCGCA GAGCAGGGCATCGACCCTGATGCCTCCACAGTGGATGAGGAGGGGCAACTGCTCGACAGC ACGGATGTTCCCTGCACAAATGGGCACGGCTCTCATAGACTGTGCATTCTGAGACAGCCG CCTGGTCAGAGGGCGGAGACCCCCAATTCCTCTGTAAGCGGTAACATCCTCTTCCCTGTC CTCAGCGAGGACTGCACTACCACAGAGGAAGGAAATCAGCCTGGAAACATGCTAAACTGC AGCCAGAACTCCAGCTCATCCTCAGTGTGGTGGCTGAAATCACCTGCATTTTCCAGTGGT TCTTCTGAGGGGGACAGCCCTTGGTCCTATCTGAATTCCAGTGGGAGTTCTTGGGTTTCA TTGCCGGGAAAGATGAGGAAAGAGATCCTTGAGGCTCGCACCTTGCAACCTGATGACTTT GAAAAGCTGTTGGCAGGAGTGAGGCATGATTGGCTGTTTCAGAGACTAGAGAATACGGGG GTTTTTAAGCCCAGTCAACTCCACCGAGCACATAGTGCTCTTTTGTTAAAATATTCAAAA AAATCTGAACTGTGGACGGCCCAGGAAACTATTGTCTATTTGGGGGACTACTTGACTGTG AAGAAAAAAGGCAGACAAAGAAATGCTTTTTGGGTTCATCATCTTCATCAAGAAGAAATT CTGGGGAGGTATGTTGGGAAAGACTATAAGGAGCAGAAGGGGCTCTGGCACCACTTCACT GATGTGGAGCGGCAGATGACCGCACAGCACTATGTGACAGAATTTAACAAGAGACTCTAT GAACAAAACATTCCCACCCAGATATTCTACATCCCATCCACAATACTACTGATTTTAGAG GACAAGACAATAAAGGGATGTATCAGTGTGGAGCCTTACATACTGGGAGAATTTGTAAAA TTGTCAAATAACACGAAAGTGGTGAAAACAGAATACAAAGCCACAGAATATGGCTTGGCC TATGGCCATTTTTCTTATGAGTTTTCTAATCATAGAGATGTTGTGGTCGATTTACAAGGT TGGGTAACCGGTAATGGAAAAGGACTCATCTACCTCACAGATCCCCAGATTCACTCCGTT GATCAGAAAGTTTTCACTACCAATTTTGGAAAGAGAGGAATTTTTTACTTCTTTAATAAC CAGCATGTGGAATGTAATGAAATCTGCCATCGTCTTTCTTTGACTAGACCTTCAATGGAG AAACCATGCACATAG Clone variation with respect to NM_025144.3 1694 g=>a;1925 a=>g;2109 t=>c;2196 g=>a;2582 t=>c;3252 a=>g >OriGene 5' read for mutant NM_025144 unedited
ACCCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGAGGGAGGTCTATATAAGCAGAGCTCGTTTAGTG AACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCTCATTCCTCAAA GATGCTGCAAATTCTAATAAATTCCCAGGTAAGGAGAGGTTGGCCTGGCTTTTTTTGTTTGTTTGTTTGT TTGTTTTGTTTTGTTTTGTTTTTGTTTTTTCAGACAGGGTCTAACTCTATTGTCCTGGATGGAGTGCAGT GGCACAATCATAATTCACTGCGACCTCAAACTCTTGGACACAAGAAATCCTCCCTCTCAGCATCCTGAGT AGCTAAGACTATAGGTAATTGATCACCCTAGACCCAGGGACACCCAATTCATCGTAATCATCATGAATAA TCAAAAAGTGGTAGCTGTGCTACTGCAAGAGTGCAAGCAAGTGCTGGATCAGCTCTTGTTGGAAGCGCCA GATGTGTCGGAGAGGACAAGAGCGAGGACCAGCGCTGCAGAGCTTTACTCCCAGCGAGTTAAGGACCCTG ATCCAGGAGCAAGGAAATGAGTGGCCCTTCGTGCTTGAAAGTGCAGTACAAACAGCGGTGGCCAAAGGAC AAACAAACTGAGATGTGATTGCGCGGTTGCAGCAGTTACTGAATTAGGTGCAAAGAGTTATTGCTCAGGA AGACAAACAGTATGGTGAATTGACTTACGCTTTCTTATCCACTCAATTTCCATCGGAGCACAATGGAAAC TTGAAAACAGGCTCTAGGCCTCACTTCCTTGACTGGCGGTCGGCATTGTTCTGGAACGTTCTGTAGCTCA CTCTGACTTCAGGTCAGTTCCATTGGCACCCACGCAATTGGCAGT >SC323654 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CMKTGAGAAAYGCAGACAAAGAAATGCTTTTTGGGTTCATCATCTTCATCAAGAAGAAATTCTGGGGAGG TATGTTGGGATGGACTATAAGGAGCAGAAGGGGCTCTGGCACCACTTCACTGATGTGGAGCGGCAGATGA CCGCACAGCACTATGTGACAGAATTTAACAAGAGACTCTATGAACAAAACATTCCCACCCAGATATTCTA CATCCCATCCACAATACTACTGATTTTAGAGGACAAGACAATAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_025144 |
| Insert Size | 5100 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_025144.2, NP_079420.2 |
| RefSeq Size | 4281 bp |
| RefSeq ORF | 3735 bp |
| Locus ID | 80216 |
| UniProt ID | Q96QP1 |
| Domains | MHCK_EF2_kinase |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes an alpha kinase. Mice which were homozygous for disrupted copies of this gene exhibited coordination defects (PMID: 21208416). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same protein (isoform 1). |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209189 | ALPK1 (Myc-DDK-tagged)-Human alpha-kinase 1 (ALPK1), transcript variant 1 |
CNY 12,992.00 |
|
| RC209189L1 | Lenti ORF clone of Human alpha-kinase 1 (ALPK1), transcript variant 1, Myc-DDK-tagged |
CNY 15,392.00 |
|
| RC209189L2 | Lenti ORF clone of Human alpha-kinase 1 (ALPK1), transcript variant 1, mGFP tagged |
CNY 11,500.00 |
|
| RC209189L3 | Lenti ORF clone of Human alpha-kinase 1 (ALPK1), transcript variant 1, Myc-DDK-tagged |
CNY 15,392.00 |
|
| RC209189L4 | Lenti ORF clone of Human alpha-kinase 1 (ALPK1), transcript variant 1, mGFP tagged |
CNY 11,500.00 |
|
| RG209189 | ALPK1 (tGFP-tagged) - Human alpha-kinase 1 (ALPK1), transcript variant 1 |
CNY 10,550.00 |
|
| SC125538 | ALPK1 (untagged)-Human alpha-kinase 1 (ALPK1), transcript variant 1 |
CNY 8,672.00 |
