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CILK1 (NM_016513) Human Untagged Clone
CAT＃: SC323626
ICK (untagged)-Kinase deficient mutant (K33M) of Human intestinal cell (MAK-like) kinase (ICK), transcript variant 2
CNY 4,712.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ECO; EJM10; hICK; ICK; LCK2; MRK |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323626 sequence for NM_016513 edited (data generated by NextGen Sequencing)
ATGAATAGATACACAACAATCAGGCAGCTCGGGGATGGAACCTACGGTTCCGTCCTGCTG GGAAGAAGCATTGAGTCTGGGGAGCTGATCGCTATTAAAAAAATGAAAAGAAAATTTTAT TCCTGGGAGGAATGCATGAACCTTCGGGAGGTTAAGTCTTTAAAGAAGCTCAACCATGCC AATGTAGTCAAATTAAAAGAAGTTATCAGGGAAAATGATCATCTTTATTTTATCTTCGAG TACATGAAGGAAAATCTTTACCAGCTCATTAAAGAGAGAAATAAGTTGTTTCCTGAGTCT GCTATAAGGAATATCATGTATCAGATATTACAAGGACTCGCATTTATTCACAAACACGGC TTCTTTCATCGAGACTTAAAGCCTGAGAACCTCCTCTGCATGGGACCAGAACTTGTGAAA ATTGCAGACTTTGGTTTGGCCCGAGAAATACGATCAAAACCTCCATATACAGATTATGTA TCTACCAGATGGTACAGGGCTCCAGAAGTACTCCTGAGGTCTACCAACTACAGCTCCCCC ATTGACGTCTGGGCGGTGGGCTGCATCATGGCAGAAGTTTACACCCTCAGGCCACTCTTC CCTGGAGCCAGTGAAATTGACACAATATTCAAAATTTGCCAAGTGCTGGGGACACCAAAA AAGACTGACTGGCCTGAAGGCTATCAACTTTCAAGTGCAATGAACTTCCGTTGGCCACAG TGTGTACCCAATAACTTAAAGACCTTGATTCCCAATGCTAGCAGTGAAGCAGTCCAGCTC CTGAGAGACATGCTTCAGTGGGATCCCAAGAAACGACCAACAGCTAGTCAGGCACTTCGA TATCCTTACTTCCAAGTTGGACACCCACTAGGCAGCACCACACAAAACCTTCAGGATTCA GAAAAACCACAGAAAGGCATCCTGGAAAAGGCAGGCCCACCTCCTTATATTAAGCCAGTC CCACCTGCCCAGCCACCAGCCAAGCCACACACACGAATTTCTTCACGACAGCATCAAGCC AGCCAGCCCCCTCTGCATCTCACGTACCCCTACAAAGCAGAGGTCTCCAGGACAGATCAC CCAAGCCATCTCCAGGAGGACAAGCCAAGCCCGTTGCTTTTCCCATCCCTCCACAACAAG CATCCACAGTCGAAAATCACAGCTGGCCTGGAGCACAAAAATGGTGAGATAAAGCCAAAG AGTAGGAGAAGGTGGGGTCTTATTTCCAGGTCAACAAAGGATTCAGATGATTGGGCTGAC TTGGATGACTTGGATTTCAGTCCATCCCTCAGCAGGATTGACCTGAAAAACAAGAAAAGA CAGAGTGATGACACTCTCTGCAGGTTTGAGAGTGTTTTGGACCTGAAGCCCTCTGAGCCT GTGGGCACAGGAAACAGTGCCCCCACCCAGACGTCATATCAGCGGCGAGACACGCCCACC CTGAGATCTGCAGCCAAGCAGCACTATTTGAAGCACTCTCGATACTTGCCTGGGATCAGT ATAAGAAATGGCATACTCTCGAATCCAGGCAAGGAATTTATTCCACCTAATCCATGGTCT AGTTCTGGCTTGTCTGGAAAATCTTCAGGGACAATGTCAGTAATCAGCAAAGTAAATTCA GTTGGTTCCAGCTCTACAAGTTCTAGTGGACTGACTGGAAACTATGTCCCTTCCTTTCTG AAAAAAGAAATCGGTTCTGCTATGCAGAGGGTACACCTAGCACCTATTCCAGACCCTTCC CCTGGTTATTCCTCCCTGAAGGCCATGAGACCTCATCCTGGGCGACCATTCTTCCACACC CAGCCTAGAAGCACTCCTGGGTTGATACCACGGCCTCCAGCCGCCCAGCCAGTGCATGGC CGGACAGACTGGGCTTCCAAGTACGCATCTCGGCGATGA Clone variation with respect to NM_016513.4 >OriGene 5' read for mutant NM_016513 unedited
CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGTGGAAAACGGCCGGG TTCCTGCTGCGCTACCATGCGCCGTGCGGCCCGTGCGACTCGCCGGACCTCGCGGGCGTCCCTGTACGGA GCCCTCGGCCGGTCCTAGCAGGGATTGTCCCCATTTCCAGCTCCGGAGCGGGCGGCTGCGCCCCGCTCGT CGAGGAGCTGCGCTCACCTCAGGGGCGGGCCCCCCGCCTGCGTTCGCGGCGCCAGCAGAAGACTGGTTGT TTGGAAATATGTATTTGGGAGACCAGTCACGTCCTTATTGAAATACCTTGTGCTGGGTGCTTGCCATCGA AAAATTCTGGTACCACTTCTGGGGGGAGGACTGCTACCACTGCAGAACTGGACCACTTCGGCCTTGAGAT GAGGGTCCGGCCTGGAGCAAGGACACCATGAATTGGTTACACACATCAGCAGCCTGGGGAAGGGAACTCA CGGTTCCGTTCTCGCGGGAAAAACCTTGAATCCGGGGACGCTTATCGATATAAGAAAAGGAAGAAAATTT TTTCGGGAGAGATGCTGACCTTCGGAAGTTAAGCTTTAAGAACTCAACAGCCATGTGTTCATTAGGAGTT TCCGGAAGTATCTTTTTTATTCCGTACTGAGGATCTTCCGCCTATAAGGAATACTGTTTCGTCGCTAAGA ATCGTTCAATTCCAGCGCTATTCAACCGGG >SC323626 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CKAMGCACATGATAGATACACAACAATCAGGCAGCTCGGGGATGGAACCTACGGTTCCGTCCTGCTGGGA AGAAGCATTGAGTCTGGGGAGCTGATCGCTATTATGAAAATGAAAAGAAAATTTTATTCCTGGGAGGAAT GCATGAACCTTCGGGAGGTTAAGTCTTTAAAGAAGCTCAACCATGCCAATGTAGTCAAATTAAAAGAAGT TATCAGGGAAAATGATCATCTTTATTTTATCTTCGAGTACATGAA |
| Restriction Sites | Please inquire |
| ACCN | NM_016513 |
| Insert Size | 3800 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016513.3, NP_057597.2 |
| RefSeq Size | 6228 bp |
| RefSeq ORF | 1899 bp |
| Locus ID | 22858 |
| UniProt ID | Q9UPZ9 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | Eukaryotic protein kinases are enzymes that belong to a very extensive family of proteins which share a conserved catalytic core common with both serine/threonine and tyrosine protein kinases. This gene encodes an intestinal serine/threonine kinase harboring a dual phosphorylation site found in mitogen-activating protein (MAP) kinases. The protein localizes to the intestinal crypt region and is thought to be important in intestinal epithelial cell proliferation and differentiation. Alternative splicing has been observed at this locus and two variants, encoding the same isoform, have been identified. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) has an additional exon in the 5' UTR, as compared to variant 1. Variants 1 and 2 encode the same protein. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216454 | ICK (Myc-DDK-tagged)-Human intestinal cell (MAK-like) kinase (ICK), transcript variant 2 |
CNY 3,990.00 |
|
| RC216454L1 | Lenti ORF clone of Human intestinal cell (MAK-like) kinase (ICK), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC216454L2 | Lenti ORF clone of Human intestinal cell (MAK-like) kinase (ICK), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC216454L3 | Lenti ORF clone of Human intestinal cell (MAK-like) kinase (ICK), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC216454L4 | Lenti ORF clone of Human intestinal cell (MAK-like) kinase (ICK), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG216454 | ICK (tGFP-tagged) - Human intestinal cell (MAK-like) kinase (ICK), transcript variant 2 |
CNY 4,370.00 |
|
| SC110272 | ICK (untagged)-Human intestinal cell (MAK-like) kinase (ICK), transcript variant 2 |
CNY 7,220.00 |
