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CDKL2 (NM_003948) Human Untagged Clone
CAT＃: SC323614
CDKL2 (untagged)-Kinase deficient mutant (K33M) of Human cyclin-dependent kinase-like 2 (CDC2-related kinase) (CDKL2)
CNY 3,656.00
CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | KKIAMRE; P56 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323614 sequence for NM_003948 edited (data generated by NextGen Sequencing)
ATGGAAAAATATGAAAACCTGGGTTTGGTTGGAGAAGGGAGTTATGGAATGGTGATGAAG TGTAGGAATAAAGATACTGGAAGAATTGTGGCCATAATGAAGTTCTTAGAAAGTGACGAT GACAAAATGGTTAAAAAGATTGCAATGCGAGAAATCAAGTTACTAAAGCAACTTAGGCAT GAAAACTTGGTGAATCTCTTGGAAGTGTGTAAGAAAAAAAAACGATGGTACCTAGTCTTT GAATTTGTTGACCACACAATTCTTGATGACTTGGAGCTCTTTCCAAATGGACTAGACTAC CAAGTAGTTCAAAAGTATTTGTTTCAGATTATTAATGGAATTGGATTTTGTCACAGTCAC AATATCATACACAGAGATATAAAGCCAGAGAATATATTAGTCTCCCAGTCTGGCGTTGTC AAGCTATGCGATTTTGGATTTGCGCGAACATTGGCAGCTCCTGGGGAGGTTTATACTGAT TATGTGGCAACCCGATGGTACAGAGCTCCAGAACTATTGGTTGGTGATGTCAAGTATGGC AAGGCTGTTGATGTGTGGGCCATTGGTTGTCTGGTAACTGAAATGTTCATGGGGGAACCC CTATTTCCTGGAGATTCTGATATTGATCAGCTATATCATATTATGATGTGTTTAGGTAAT CTAATTCCAAGGCATCAGGAGCTTTTTAATAAAAATCCTGTGTTTGCTGGAGTAAGGTTG CCTGAAATCAAGGAAAGAGAACCTCTTGAAAGACGCTATCCTAAGCTCTCTGAAGTGGTG ATAGATTTAGCAAAGAAATGCTTACATATTGACCCCGACAAAAGACCCTTCTGTGCTGAG CTCCTACACCATGATTTCTTTCAAATGGATGGATTTGCTGAGAGGTTTTCCCAAGAACTA CAGTTAAAAGTACAGAAAGATGCCAGAAATGTTTCTTTATCTAAAAAATCCCAAAACAGA AAGAAGGAAAAAGAAAAAGATGATTCCTTAGTTGAAGAAAGAAAAACACTTGTGGTACAG GATACCAATGCTGATCCCAAAATTAAGGATTATAAACTATTTAAAATAAAAGGCTCAAAA ATTGATGGAGAAAAAGCTGAAAAAGGCAATAGAGCTTCAAATGCCAGCTGTCTCCATGAC AGTAGGACAAGCCACAACAAAATAGTGCCTTCAACAAGCCTCAAAGACTGCAGCAATGTC AGCGTGGACCACACAAGGAATCCAAGCGTGGCAATTCCCCCACTTACACACAATCTTTCT GCAGTTGCTCCCAGCATTAATTCTGGAATGGGGACTGAGACTATACCAATTCAGGGTTAC AGAGTGGATGAGAAAACTAAGAAGTGTTCTATTCCATTTGTTAAACCGAACAGACATTCC CCATCAGGCATTTATAACATTAATGTGACCACATTAGTATCAGGACCTCCCCTGTCAGAT GATTCAGGGGCTGATTTGCCTCAAATGGAACACCAGCACTGA Clone variation with respect to NM_003948.3 98 a=>t >OriGene 5' read for mutant NM_003948 unedited
CCCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGAGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCAGGCTCCAGGT ACTGGGCGCCTTACGAGCTGGGAGGTGGTGCCTCTCACCCAGCTAATTGCTCTCTAGCCCTTGGCCTTCA CAGGTGTTGGTGCCTGCCGTGAACGCATTCTGACCTGGGCCGTATCTGTCTCCCAAGACTTTGTGCCTAT GGTTGGGGACAGAGTGAGGTCGTTGCCTTGACGACGACAGCATGCGGCCCGTGGTCCTCCTAAGTGTGAG CTTGCGGCGGACCGAGGCCCACCTGCCTCCCTGCCTGCTTCGCCCTGGACTCGTGACTGCGTCCGCAGAG AATCACAACAGCGCTGGATTTGCTAGTTTGCTAGGCAGCATCTTTGGACCTGCGAACCATATGCATTTCA CCTCAAATTTGTTTCCAAGTTGAAAACCTTTGGGTCTTTCTATGCGAACGGATTGAAGAACCGCAAAAGT TCTACGGACTTTAAATAAATGGAAAAATTATGAAAACCTGGGTAGTGGAAAATGAGTTATGATGGTGAAT GAATGTGTAGAATAAAGATCCTGGAAGATTGGGCCTATATGGAGTTCTAAAATGTGCCAATAGCACAACG GGTTAAAGAATGCATGCCGAAAATCCAGTTTCATAGCCACTTAGGCTGGAACCTTGTGGAATCTCTTGGA ACGTTGAAAAAAAAACCAATGGCTCATGCCTGGAATTGTGTACCACCATCTTGAGACTGGGACCCTTCCA TGAGCATCAGTCACCGAAGTTCAGACTGTCGACAATTATAGAGCGTAGTTGCTGACGCACCTACACGAAA TGACCGAGAATCGTATTTCACTTGCGCTATATCGACACTTGATTGTACA >SC323614 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
GAAACCTGMGGTTTGGTTGGAGAAGGGAGTTATGGAATGGTGATGAAGTGTAGGAATAAAGATMCTYGAA GAATTGTGGCCATAATGAAGTTCTTAGAAAGTGACGATGACAAAATGGTTAAAAAGATTGCAATGCGAGA AATCAAGTTACTAAAGCAACTTAGGCATGAAAACTTGGTGAATCTCTTGGAAGTGTGTAAGAAAAAAAAA CGATGGTACCTAGTCTTTGAATTTGTTGACCACACAATTCTTGAT |
| Restriction Sites | Please inquire |
| ACCN | NM_003948 |
| Insert Size | 4700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003948.2, NP_003939.1 |
| RefSeq Size | 1993 bp |
| RefSeq ORF | 1482 bp |
| Locus ID | 8999 |
| UniProt ID | Q92772 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene product is a member of a large family of CDC2-related serine/threonine protein kinases. It accumulates primarily in the cytoplasm, with lower levels in the nucleus. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210780 | CDKL2 (Myc-DDK-tagged)-Human cyclin-dependent kinase-like 2 (CDC2-related kinase) (CDKL2) |
CNY 3,656.00 |
|
| RC210780L1 | Lenti ORF clone of Human cyclin-dependent kinase-like 2 (CDC2-related kinase) (CDKL2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210780L2 | Lenti ORF clone of Human cyclin-dependent kinase-like 2 (CDC2-related kinase) (CDKL2), mGFP tagged |
CNY 5,890.00 |
|
| RC210780L3 | Lenti ORF clone of Human cyclin-dependent kinase-like 2 (CDC2-related kinase) (CDKL2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210780L4 | Lenti ORF clone of Human cyclin-dependent kinase-like 2 (CDC2-related kinase) (CDKL2), mGFP tagged |
CNY 5,890.00 |
|
| RG210780 | CDKL2 (tGFP-tagged) - Human cyclin-dependent kinase-like 2 (CDC2-related kinase) (CDKL2) |
CNY 4,370.00 |
|
| SC111095 | CDKL2 (untagged)-Human cyclin-dependent kinase-like 2 (CDC2-related kinase) (CDKL2) |
CNY 3,656.00 |
