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FES (NM_002005) Human Untagged Clone
CAT＃: SC323596
FES (untagged)-Kinase deficient mutant (K590M) of Human feline sarcoma oncogene (FES), transcript variant 1
CNY 6,032.00
CNY 7,220.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | FPS |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323596 sequence for NM_002005 edited (data generated by NextGen Sequencing)
ATGGGCTTCTCTTCCGAGCTGTGCAGCCCCCAGGGCCACGGGGTCCTGCAGCAAATGCAG GAGGCCGAGCTTCGTCTACTGGAGGGCATGAGAAAGTGGATGGCCCAGCGGGTCAAGAGT GACAGGGAGTATGCAGGACTGCTTCACCACATGTCCCTGCAGGACAGTGGGGGCCAGAGC CGGGCCATCAGCCCTGACAGCCCCATCAGTCAGTCCTGGGCTGAGATCACCAGCCAAACT GAGGGCCTGAGCCGCTTGCTGCGGCAGCACGCAGAGGATCTGAACTCAGGGCCCCTGAGC AAGCTGAGCCTGCTCATCCGGGAACGGCAGCAGCTTCGCAAGACCTACAGCGAGCAGTGG CAGCAGCTGCAGCAGGAGCTCACCAAGACCCACAGCCAGGACATTGAGAAGCTGAAGAGC CAGTACCGAGCTCTGGCACGGGACAGTGCCCAAGCCAAGCGCAAGTACCAGGAGGCCAGC AAAGACAAGGACCGTGACAAGGCTAAGGACAAGTATGTGCGCAGCCTGTGGAAGCTCTTT GCTCACCACAACCGCTATGTGCTGGGCGTGCGGGCTGCGCAGCTACACCACCAGCACCAC CACCAGCTCCTGCTGCCCGGCCTGCTGCGGTCACTGCAGGACCTGCACGAGGAGATGGCT TGCATCCTGAAGGAGATCCTGCAGGAATACCTGGAGATTAGCAGCCTGGTGCAGGATGAG GTGGTGGCCATTCACCGGGAGATGGCTGCAGCTGCTGCCCGCATCCAGCCTGAGGCTGAG TACCAAGGCTTCCTGCGACAGTATGGGTCCGCACCTGACGTCCCACCCTGTGTCACGTTC GATGAGTCACTGCTTGAGGAGGGTGAACCGCTGGAGCCTGGGGAGCTCCAGCTGAACGAG CTGACTGTGGAGAGCGTGCAGCACACGCTGACCTCAGTGACAGATGAGCTGGCTGTGGCC ACCGAGATGGTGTTCAGGCGGCAGGAGATGGTTACGCAGCTGCAACAGGAGCTCCGGAAT GAAGAGGAGAACACCCACCCCCGGGAGCGGGTGCAGCTGCTGGGCAAGAGGCAAGTGCTG CAAGAAGCACTGCAGGGGCTGCAGGTAGCGCTGTGCAGCCAGGCCAAGCTGCAGGCCCAG CAGGAGTTGCTGCAGACCAAGCTGGAGCACCTGGGCCCCGGCGAGCCCCCGCCTGTGCTG CTCCTGCAGGATGACCGCCACTCCACGTCGTCCTCGGAGCAGGAGCGAGAGGGGGGAAGG ACACCCACGCTGGAGATCCTTAAGAGCCACATCTCAGGAATCTTCCGCCCCAAGTTCTCG CTCCCTCCACCGCTGCAGCTCATTCCGGAGGTGCAGAAGCCCCTGCATGAGCAGCTGTGG TACCACGGGGCCATCCCGAGGGCAGAGGTGGCTGAGCTGCTGGTGCACTCTGGGGACTTC CTGGTGCGGGAGAGCCAGGGCAAGCAGGAGTACGTGCTGTCGGTGCTGTGGGATGGTCTG CCCCGGCACTTCATCATCCAGTCCTTGGATAACCTGTACCGACTGGAAGGGGAAGGCTTT CCTAGCATTCCTTTGCTCATCGACCACCTACTGAGCACCCAGCAGCCCCTCACCAAGAAG AGTGGTGTTGTCCTGCACAGGGCTGTGCCCAAGGACAAGTGGGTGCTGAACCATGAGGAC CTGGTGTTGGGTGAGCAGATTGGACGGGGGAACTTTGGCGAAGTGTTCAGCGGACGCCTG CGAGCCGACAACACCCTGGTGGCGGTGAAGTCTTGTCGAGAGACGCTCCCACCTGACCTC AAGGCCAAGTTTCTACAGGAAGCGAGGATCCTGAAGCAGTACAGCCACCCCAACATCGTG CGTCTCATTGGTGTCTGCACCCAGAAGCAGCCCATCTACATCGTCATGGAGCTTGTGCAG GGGGGCGACTTCCTGACCTTCCTCCGCACGGAGGGGGCCCGCCTGCGGGTGAAGACTCTG CTGCAGATGGTGGGGGATGCAGCTGCTGGCATGGAGTACCTGGAGAGCAAGTGCTGCATC CACCGGGACCTGGCTGCTCGGAACTGCCTGGTGACAGAGAAGAATGTCCTGAAGATCAGT GACTTTGGGATGTCCCGAGAGGAAGCCGATGGGGTCTATGCAGCCTCAGGGGGCCTCAGA CAAGTCCCCGTGAAGTGGACCGCACCTGAGGCCCTTAACTACGGCCGCTACTCCTCCGAA AGCGACGTGTGGAGCTTTGGCATCTTGCTCTGGGAGACCTTCAGCCTGGGGGCCTCCCCC TATCCCAACCTCAGCAATCAGCAGACACGGGAGTTTGTGGAGAAGGGGGGCCGTCTGCCC TGCCCAGAGCTGTGTCCTGATGCCGTGTTCAGGCTCATGGAGCAGTGCTGGGCCTATGAG CCTGGGCAGCGGCCCAGCTTCAGCACCATCTACCAGGAGCTGCAGAGCATCCGAAAGCGG CATCGGTGA Clone variation with respect to NM_002005.3 >OriGene 5' read for mutant NM_002005 unedited
ACCGCACGTTGAGCAATGGGCGGTAGGCGTGTACGGAGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCAGGAGCAGCTGCC CGTGCGGGTACCTCTAGCCCCGGGGCCTGGAGGAGCGGTGGGAGCTGGGGGCGCGGCAGGCAGGGGCAGA GCAGGCGTTCCGAGGGCCAGAGACCCACCCAGAACAGCACTATGGGCTTCTCTTCCGAGCTGTGCAGCCC CCAGGGCCACGGGGTCCTGCAGCAAATGCAGGAGGCCGAGCTTCGTCTACTGGAGGGCATGAGAAAAGTG GATGGCCCCAGCGGGTCAAGAGTGACAGGGAGTATGCAGGACTGCTTTAACCACATGTCCCTGCAGAAAT GGGGGGCCAGAGCCGGCCCTTCACCCTTGACAGCCCCATCATTCATTCCGGGGTTGAGATACCCAGCCAA CTTGAGGCCCTGACCCGCTTTGCTCCGGCAGCACGCAAAGGATCTGACTTCAGGGCCCTTGACCAAGCTG ACCCTGCTATTCGGGAACGGGCAACGCGCTTCCGCAGAAACCTACAGAGACATGTGACACCACTCTGAAA CGAGATCTACCCGAGACCCCACGCCGGGACTTGGAAAGCTAAAGACCCTGTCCAGATCTTGGGCCGGGGA CAGGCCCCAGCCACGCCAGTCCAGAGGCCACAAACAGGCCCGTAACGGGCCAGGAAATTGTGCGCCTCGG AAATCTTTTGCTCACACACTTTTTCTGGGTTGGGTTGGCAATCACAAAGCCCACATTTTGGTGGCGCTGG GGGGTCATGACACCAGAAGAGATGCTACTCTGACTCAGGTACTCCCAGTGCACTAGAACCCCTTGAGAGG AAGCTGTCGCGTAGAAACTTCAGGACGATCTT >SC323596 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
ATGGKACAGATGGMGGGGACTTTGGCGAGTGTTCAGCGGACGCCTGCGAGCCGACAACACCCTGGTGGCG GTGATGTCTTGTCGAGAGACGCTCCCACCTGACCTCAAGGCCAAGTTTCTACAGGAAGCGAGGATCCTGA AGCAGTACAGCCACCCCAACATCGTGCGTCTCATTGGTGTCTGCACCCAGAAGCAGCCCATCTACATCGT CATGGAGCTTGTGCAGGGGGGCGACTTCCTGACCTTCCTCCGCAC |
| Restriction Sites | Please inquire |
| ACCN | NM_002005 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002005.2, NP_001996.1 |
| RefSeq Size | 2762 bp |
| RefSeq ORF | 2469 bp |
| Locus ID | 2242 |
| UniProt ID | P07332 |
| Domains | pkinase, SH2, FCH, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Axon guidance |
| Gene Summary | This gene encodes the human cellular counterpart of a feline sarcoma retrovirus protein with transforming capabilities. The gene product has tyrosine-specific protein kinase activity and that activity is required for maintenance of cellular transformation. Its chromosomal location has linked it to a specific translocation event identified in patients with acute promyelocytic leukemia but it is also involved in normal hematopoiesis as well as growth factor and cytokine receptor signaling. Alternative splicing results in multiple variants encoding different isoforms.[provided by RefSeq, Jan 2009] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
CD84 Negatively Regulates IgE High-Affinity Receptor Signaling in Human Mast Cells
,Damiana Álvarez-Errico, Irene Oliver-Vila, Erola Ainsua-Enrich, Alasdair M. Gilfillan, César Picado, Joan Sayós, and Margarita Martín,
J. Immunol., Dec 2011; 187: 5577 - 5586.
[FES]
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|
Identification of somatic JAK1 mutations in patients with acute myeloid leukemia
,Zhifu Xiang, Yu Zhao, Vesselin Mitaksov, Daved H. Fremont, Yumi Kasai, AnnaLynn Molitoris, Rhonda E. Ries, Tracie L. Miner, Michael D. McLellan, John F. DiPersio, Daniel C. Link, Jacqueline E. Payton, Timothy A. Graubert, Mark Watson, William Shannon, Sharon E. Heath, Rakesh Nagarajan, Elaine R. Mardis, Richard K. Wilson, Timothy J. Ley, and Michael H. Tomasson,
Blood. 2008; 111(9): p. 4809-4812
[FES]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206408 | FES (Myc-DDK-tagged)-Human feline sarcoma oncogene (FES), transcript variant 1 |
CNY 3,990.00 |
|
| RC206408L1 | Lenti-ORF clone of FES (Myc-DDK-tagged)-Human feline sarcoma oncogene (FES), transcript variant 1 |
CNY 5,890.00 |
|
| RC206408L2 | Lenti-ORF clone of FES (mGFP-tagged)-Human feline sarcoma oncogene (FES), transcript variant 1 |
CNY 5,890.00 |
|
| RC206408L3 | Lenti-ORF clone of FES (Myc-DDK-tagged)-Human feline sarcoma oncogene (FES), transcript variant 1 |
CNY 5,890.00 |
|
| RC206408L4 | Lenti-ORF clone of FES (mGFP-tagged)-Human feline sarcoma oncogene (FES), transcript variant 1 |
CNY 5,890.00 |
|
| RG206408 | FES (tGFP-tagged) - Human feline sarcoma oncogene (FES), transcript variant 1 |
CNY 4,370.00 |
|
| SC118883 | FES (untagged)-Human feline sarcoma oncogene (FES), transcript variant 1 |
CNY 2,950.00 |
