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MAP4K5 (NM_006575) Human Untagged Clone
CAT＃: SC323591
MAP4K5 (untagged)-Kinase deficient mutant (K49M) of Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 1
CNY 6,208.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GCKR; KHS; KHS1; MAPKKKK5 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_006575, the custom clone sequence may differ by one or more nucleotides
ATGGAGGCCCCGCTGCGGCCTGCCGCGGACATCCTGAGGCGGAACCCGCAGCAGGACTACGAACTCGTCC AGAGGGTCGGCAGCGGCACCTACGGGGACGTCTATAAGGCCAGAAATGTACACACAGGAGAGCTGGCTGC AGTAAAAATCATTAAATTGGAGCCTGGAGATGATTTTTCTTTGATTCAACAAGAAATATTTATGGTTAAA GAATGTAAACATTGTAACATCGTTGCCTACTTTGGGAGTTATCTTAGTCGGGAAAAACTATGGATTTGTA TGGAATACTGTGGTGGCGGATCACTTCAAGATATTTACCATGTTACTGGACCATTATCAGAATTGCAAAT AGCCTATGTATGCAGAGAAACCTTACAGGGTCTTGCCTATTTGCATACTAAAGGCAAAATGCATAGAGAT ATCAAAGGTGCTAATATTTTATTGACAGACCATGGCGATGTAAAATTAGCTGACTTTGGTGTGGCTGCAA AAATAACAGCTACCATTGCAAAACGAAAATCTTTCATTGGCACCCCTTACTGGATGGCCCCAGAAGTTGC AGCAGTAGAGAAGAATGGTGGCTACAACCAACTCTGTGATATCTGGGCAGTAGGAATAACAGCAATTGAA CTTGGAGAACTTCAGCCACCTATGTTTGATCTCCACCCAATGAGGGCTCTCTTCTTAATGTCAAAAAGTA ATTTTCAGCCTCCAAAACTAAAGGACAAAACAAAATGGTCATCAACATTCCATAATTTTGTCAAAATAGC ACTAACCAAAAACCCAAAAAAAAGACCAACTGCTGAAAGACTTCTGACTCACACTTTTGTTGCACAGCCA GGTCTCTCTAGAGCCCTAGCAGTTGAACTGTTAGACAAAGTGAACAATCCAGATAACCACGCACATTACA CTGAAGCAGATGACGATGACTTTGAGCCCCATGCAATCATTCGTCATACCATTAGATCTACAAACAGGAA TGCCAGAGCTGAACGGACAGCTTCAGAAATAAATTTTGACAAATTACAATTTGAACCTCCTCTGAGAAAA GAAACAGAAGCACGAGATGAAATGGGATTGTCATCAGACCCAAATTTCATGTTACAGTGGAATCCTTTTG TTGATGGTGCAAATACTGGCAAATCAACCTCAAAACGTGCAATACCACCTCCCCTACCTCCTAAGCCAAG GATAAGCAGTTACCCTGAAGACAACTTTCCGGATGAAGAAAAAGCATCAACCATAAAACATTGTCCTGAT TCAGAAAGCAGAGCTCCCCAAATTCTCAGAAGACAGAGTAGCCCAAGTTGTGGGCCTGTGGCAGAGACTT CTTCTATTGGAAATGGTGATGGTATTTCAAAACTGATGAGTGAAAATACAGAAGGATCAGCACAAGCACC ACAGTTACCACGAAAAAAGGACAAACGAGACTTCCCTAAACCAGCCATCAATGGCCTTCCACCCACCCCA AAAGTTCTGATGGGAGCATGCTTTTCAAAAGTTTTTGATGGCTGTCCTTTGAAAATTAATTGTGCAACAT CCTGGATACATCCTGATACAAAAGATCAGTACATTATTTTTGGAACTGAAGATGGTATTTACACACTGAA TCTCAATGAGCTACATGAGGCAACGATGGAACAGTTATTTCCACGGAAGTGTACTTGGCTGTATGTTATC AATAATACTTTAATGTCATTATCAGAAGGAAAAACCTTTCAGCTCTACTCTCACAATCTTATAGCTTTGT TTGAACATGCCAAAAAACCAGGATTAGCTGCCCATATTCAAACTCACAGGTTTCCAGACCGAATACTACC AAGAAAATTCGCTTTAACAACAAAGATTCCTGATACAAAAGGCTGCCACAAATGTTGCATAGTCAGAAAC CCTTACACGGGACATAAATACCTCTGTGGAGCTTTACAGTCTGGAATTGTTTTACTTCAGTGGTATGAGC CAATGCAGAAATTCATGTTGATAAAGCACTTTGATTTTCCTTTGCCAAGTCCTTTGAATGTTTTTGAAAT GCTGGTGATACCTGAACAGGAATACCCTATGGTCTGTGTAGCTATTAGCAAAGGCACTGAATCGAATCAG GTAGTTCAGTTTGAGACAATCAATTTGAACTCTGCATCTTCATGGTTTACAGAAATTGGTGCAGGCAGCC AGCAGTTAGATTCCATTCATGTAACACAGTTGGAGAGAGATACCGTTTTAGTGTGTTTAGACAAATTTGT GAAAATTGTAAATCTACAAGGAAAATTAAAATCAAGTAAGAAACTGGCCTCTGAGTTAAGTTTTGATTTT CGCATTGAATCTGTAGTATGCCTTCAAGACAGTGTGTTGGCTTTCTGGAAACATGGGATGCAGGGTAAAA GCTTCAAGTCAGATGAGGTTACCCAGGAGATTTCAGATGAAACAAGAGTTTTCCGCTTATTAGGATCAGA CAGGGTTGTCGTTTTGGAAAGTAGGCCAACAGAAAATCCTACTGCACACAGCAATCTCTACATCTTGGCT GGACATGAAAATAGTTACTAA >OriGene 5' read for mutant NM_006575 unedited
CCCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGTGGAGGTCTATATAAGCAGAGCTCGTTTAGTG AACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCGGCGGCGACTGT ACGCGCCTCCGCCGCCCCCGAGAGGACGCGCCGTGCAGCGGCTGAGTGGCGGCGGCGGCGACGGCAAACC CGGAGCTGCCGGCCGGCGCGCGGGAGGAGGACGCGGGTGCGGTCTAGGAAACGGAGCTGCGGGCGGAGGC TCCATGTTGGGAAGCGGCGCCGTTCGTGCTTGTTAGCGGGAATCCGGGAGCCGCGGGGTGAGCTGGCCGG GGGGCCCGGGCCCTAAGTGAAGATGGAAGGCCCCCGCTGGCGGCCCTGCCGGCGGACATCCTGAGGCGGA CCCCGCAGCAGGACTTACGACCTCGTCCAGGAGGGTCGGCACCGGCACCTAACGGGGACGTCCTATAAGG CCGAAATGTACACACAGGAGAGCCTGGCTTGAATAATGAATCATTAAATTGAACCTGGAAGGTTATTTTC TTGAATTACACAGAATTTTTATGGTAAGAATGTACCATTGAACATCGGTGCATCTTGGGGAGTACTTATC GGAAACCTGGATGTAGGATACGGGGGGGTCTCAATTTCCAGTTCTGACATTCGATGCAATTCCTAGTTCG AAACTTAAGGTCTGCAATTGCTCACGCACCGTGAATCAGGTCTTTTTTTGGCACTCGGCGATAATAACAA TAGGTCAAATCCACTTGAAAAAATT >SC323591 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
ARTCAGAGGGTCGGAGCGGCACCTACGGGGACGTCTATAAGGCCAGAAATGTACACACAGGAGAGCTGGC TGCAGTAATGATCATTAAATTGGAGCCTGGAGATGATTTTTCTTTGATTCAACAAGAAATATTTATGGTT AAAGAATGTAAACATTGTAACATCGTTGCCTACTTTGGGAGTTATCTTAGTCGGGAAAAACTATGGATTT GTATGGAATACTGTGGTGGCGGATCACTTCAAGATATTTACCATG |
| Restriction Sites | Please inquire |
| ACCN | NM_006575 |
| Insert Size | 4310 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006575.3, NP_006566.2 |
| RefSeq Size | 4373 bp |
| RefSeq ORF | 2541 bp |
| Locus ID | 11183 |
| UniProt ID | Q9Y4K4 |
| Domains | pkinase, CNH |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Variants 1 and 2 encode the same isoform. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217191 | MAP4K5 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 1 |
CNY 3,990.00 |
|
| RC217191L1 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC217191L2 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC217191L3 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC217191L4 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG217191 | MAP4K5 (tGFP-tagged) - Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 1 |
CNY 4,370.00 |
|
| SC110237 | MAP4K5 (untagged)-Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 1 |
CNY 2,950.00 |
