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TAOK2 (NM_004783) Human Untagged Clone
CAT＃: SC323586
TAOK2 (untagged)-Kinase deficient mutant (K57M) of Human TAO kinase 2 (TAOK2), transcript variant 1
CNY 7,312.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MAP3K17; PSK; PSK1; PSK1-BETA; TAO1; TAO2 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323586 sequence for NM_004783 edited (data generated by NextGen Sequencing)
ATGCCAGCTGGGGGCCGGGCCGGGAGCCTGAAGGACCCAGATGTGGCTGAGCTCTTCTTC AAGGATGACCCAGAAAAGCTCTTCTCTGACCTCCGGGAAATTGGCCATGGCAGCTTTGGA GCCGTATACTTTGCCCGGGATGTCCGGAATAGTGAGGTGGTGGCCATCAWGAAGATGTCC TACAGTGGGAAGCAGTCCAATGAGAAATGGCAAGACATCATCAAGGAGGTGCGGTTCTTA CAGAAGCTCCGGCATCCCAACACCATTCAGTACCGGGGCTGTTACCTGAGGGAGCACACG GCTTGGCTGGTAATGGAGTATTGCCTGGGCTCAGCTTCTGACCTTCTAGAAGTGCACAAG AAACCCCTTCAGGAGGTAGAGATCGCAGCTGTGACCCACGGGGCGCTTCAGGGCCTGGCA TATCTGCACTCCCACAACATGATCCATAGGGATGTGAAGGCTGGAAACATCCTGCTGTCA GAGCCAGGGTTAGTGAAGCTAGGGGACTTTGGTTCTGCGTCCATCATGGCACCTGCCAAC TCCTTCGTGGGCACCCCATACTGGATGGCACCCGAGGTGATCCTGGCCATGGATGAGGGG CAGTACGATGGCAAAGTGGACGTCTGGTCCTTGGGGATAACCTGCATCGAGCTGGCTGAA CGGAAACCACCGCTCTTTAACATGAATGCGATGAGTGCCTTATACCACATTGCACAGAAC GAATCCCCCGTGCTCCAGTCAGGACACTGGTCTGAGTACTTCCGGAATTTTGTCGACTCC TGTCTTCAGAAAATCCCTCAAGACAGACCAACCTCAGAGGTTCTCCTGAAGCACCGCTTT GTGCTCCGGGAGCGGCCACCCACAGTCATCATGGACCTGATCCAGAGGACCAAGGATGCC GTGCGGGAGCTGGACAACCTGCAGTACCGCAAGATGAAGAAGATCCTGTTCCAAGAGGCA CCCAACGGCCCTGGTGCCGAGGCCCCAGAGGAGGAAGAGGAGGCCGAGCCCTACATGCAC CGGGCCGGGACTCTGACCAGCCTCGAGAGTAGCCACTCAGTGCCCAGCATGTCCATCAGC GCCTCCAGCCAGAGCAGCTCCGTCAACAGCCTAGCAGATGCCTCAGACAACGAGGAAGAG GAGGAGGAGGAGGAGGAAGAGGAGGAGGAGGAAGAAGGCCCTGAAGCCCGGGAGATGGCC ATGATGCAGGAGGGGGAGCACACAGTCACCTCTCACAGCTCCATTATCCACCGGCTGCCG GGCTCTGACAACCTATATGATGACCCCTACCAGCCAGAGATAACCCCCAGCCCTCTCCAG CCGCCTGCAGCCCCAGCTCCCACTTCCACCACCTCTTCCGCCCGCCGCCGGGCCTACTGC CGTAACCGAGACCACTTTGCCACCATCCGAACCGCCTCCCTGGTCAGCCGTCAGATCCAG GAGCATGAGCAGGACTCTGCGCTGCGGGAGCAGCTGAGCGGCTATAAGCGGATGCGACGA CAGCACCAGAAGCAGCTGCTGGCCCTGGAGTCACGGCTGAGGGGTGAACGGGAGGAGCAC AGTGCACGGCTGCAGCGGGAGCTTGAGGCGCAGCGGGCTGGCTTTGGGGCAGAGGCAGAA AAGCTGGCCCGGCGGCACCAGGCCATAGGTGAGAAGGAGGCACGAGCTGCCCAGGCCGAG GAGCGGAAGTTCCAGCAGCACATCCTTGGGCAGCAGAAGAAGGAGCTGGCTGCCCTGCTG GAGGCACAGAAGCGGACCTACAAACTTCGCAAGGAACAGCTGAAGGAGGAGCTCCAGGAG AACCCCAGCACTCCCAAGCGGGAGAAGGCCGAGTGGCTGCTGCGGCAGAAGGAGCAGCTC CAGCAGTGCCAGGCGGAGGAGGAAGCAGGGCTGCTGCGGCGGCAGCGCCAGTACTTTGAG CTGCAGTGTCGCCAGTACAAGCGCAAGATGTTGCTGGCTCGGCACAGCCTGGACCAGGAC CTGCTGCGGGAGGACCTGAACAAGAAGCAGACCCAGAAGGACTTGGAGTGTGCACTGCTG CTTCGGCAGCACGAGGCCACGCGGGAGCTGGAGCTGCGGCAGCTCCAGGCCGTGCAGCGC ACGCGGGCTGAGCTCACCCGCCTGCAGCACCAGACGGAGCTGGGCAACCAGCTGGAGTAC AACAAGCGGCGTGAGCAAGAGTTGCGGCAGAAGCATGCGGCCCAGGTTCGCCAGCAGCCC AAGAGCCTCAAATCTAAGGAGCTGCAGATCAAGAAGCAGTTCCAGGAGACGTGTAAGATC CAGACTCGGCAGTACAAGGCTCTGCGAGCACACTTGCTGGAGACCACGCCCAAAGCTCAG CACAAGAGCCTCCTTAAGCGGCTCAAGGAAGAGCAGACCCGCAAGCTGGCGATCTTGGCG GAGCAGTATGACCAGTCCATCTCAGAGATGCTCAGCTCACAGGCGCTGCGGCTTGATGAG ACCCAGGAGGCAGAGTTCCAGGCCCTTCGGCAGCAGCTTCAACAGGAGCTGGAGCTGCTC AACGCTTACCAGAGCAAGATCAAGATCCGCACAGAGAGCCAGCACGAGAGGGAGCTGCGG GAGCTGGAGCAGAGGGTCGCGCTGCGGCGGGCACTGCTGGAGCAGCGGGTGGAAGAGGAG CTGCTGGCCCTGCAGACAGGACGCTCCGAGCGAATCCGCAGTCTGCTTGAGCGGCAGGCC CGTGAGATCGAGGCCTTCGATGCGGAAAGCATGAGGCTGGGCTTCTCCAGCATGGCTCTG GGGGGCATCCCGGCTGAAGCTGCTGCCCAGGGCTATCCTGCTCCACCCCCTGCCCCAGCC TGGCCCTCCCGTCCCGTTCCCCGTTCTGGGGCACACTGGAGCCATGGCCCTCCTCCACCA GGCATGCCCCCTCCAGCCTGGCGTCAGCCGTCTCTGCTGGCTCCCCCAGGCCCCCCAAAC TGGCTGGGGCCCCCCACACAAAGTGGGACACCCCGTGGCGGAGCCCTGCTGCTGCTAAGA AACAGCCCCCAGCCCCTGCGGCGGGCAGCCTCGGGGGGCAGTGGCAGTGAGAATGTGGGC CCCCCTGCTGCCGCGGTGCCCGGGCCCCTGAGCCGCAGCACCAGTGTCGCTTCCCACATC CTCAATGGTTCTTCCCACTTCTATTCCTGA Clone variation with respect to NM_004783.2 170 a=>w >OriGene 5' read for mutant NM_004783 unedited
ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACAGGGAAGGAGGTCTATATAAGCAGAGCTCGTTTAGTG AACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTGACCCCTACCA GGCCAGGCCCCACTCTCAGGGCCCCCAGGGGCCACCATGCCAGCTGGGGGCCGGGCCGGGAGCCTGAAGG ACCCAGATGTGGCTGAGCTCTTCTTCAAGGATGACCCAGAAAAGCTCTTCTCTGACCTCCGGGAAATTGG CCATGGCAGCTTTGGAGCCGTATACTTTGCCCGGGATGTCCGGAATAGTGAGGTGGTGGCCATCATGAAG ATGTCCTACAGTGGGAAGCAGTCCAATGAGAAATGGCAAGACATCATCAAGGAGGTGCGGTTCTTACAGA GCTCCGGCATCCCAACACCATTCAGTACCGGGGCTGTTACCTGAGGAGCACACGGCTTGCTGTAATGAGT ATGCCTGGGCTCAGCTCTGACCTTCTAGATGCCACAGAACCCTCAGGAGGTAAAGAGATCCGCAGCTTGT GACCCACCGGGCCGCTTCAGGCCTGGCATACTGCCTCCCACCACTTGATCATAGGATGTGAAGCCTGAAA CATCTGCTGCCAGAGCCAGGGTTAGTGAACTCTAGGAACCTAGTCCGCTCACATCAGGGACACTCTGGCC AATCTCCTTCTGGACACTATCTGTGGATGGCCCAGTGTATTCTGCTATGTAAGGGCTATCAATGGCCAAT GGACCTTGCTCTGATAATGCCGTGCTAACGAGTCTGTCAAGCAGAACACGCGCATATAACGAATAGATCG AATGAGTGCATAACATGTGCACGGACTATCCCGTCCATATAAGACAGCGTGTGATTCGAGATTTTGCAAC ATGCTTCAAACTCCCGAGGAAC >SC323586 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CMGWGACCRTAAGCTSTTCTCTGACCTCCGGGAATTGGCCATGGCAGCTTTGGAGCCGTATACTTTGCCC GGGATGTCCGGAATAGTGAGGTGGTGGCCATCATGAAGATGTCCTACAGTGGGAAGCAGTCCAATGAGAA ATGGCAAGACATCATCAAGGAGGTGCGGTTCTTACAGAAGCTCCGGCATCCCAACACCATTCAGTACCGG GGCTGTTACCTGAGGGAGCACACGGCTTGGCTGGTAATGGAGTAT |
| Restriction Sites | Please inquire |
| ACCN | NM_004783 |
| Insert Size | 4000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004783.2, NP_004774.1 |
| RefSeq Size | 4625 bp |
| RefSeq ORF | 3150 bp |
| Locus ID | 9344 |
| UniProt ID | Q9UL54 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase, Transmembrane |
| Protein Pathways | MAPK signaling pathway |
| Gene Summary | This gene encodes a serine/threonine protein kinase that is involved in many different processes, including, cell signaling, microtubule organization and stability, and apoptosis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011] Transcript Variant: This variant (2) contains alternate exons at the 3' end compared to variant 1. This results in a shorter isoform (2, also known as PSK1-beta) with a distinct C-terminus compared to isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214344 | TAOK2 (Myc-DDK-tagged)-Human TAO kinase 2 (TAOK2), transcript variant 1 |
CNY 7,304.00 |
|
| RC214344L1 | Lenti ORF clone of Human TAO kinase 2 (TAOK2), transcript variant 1, Myc-DDK-tagged |
CNY 9,704.00 |
|
| RC214344L2 | Lenti ORF clone of Human TAO kinase 2 (TAOK2), transcript variant 1, mGFP tagged |
CNY 12,350.00 |
|
| RC214344L3 | Lenti ORF clone of Human TAO kinase 2 (TAOK2), transcript variant 1, Myc-DDK-tagged |
CNY 9,704.00 |
|
| RC214344L4 | Lenti ORF clone of Human TAO kinase 2 (TAOK2), transcript variant 1, mGFP tagged |
CNY 9,704.00 |
|
| RG214344 | TAOK2 (tGFP-tagged) - Human TAO kinase 2 (TAOK2), transcript variant 1 |
CNY 8,904.00 |
|
| SC117141 | TAOK2 (untagged)-Human TAO kinase 2 (TAOK2), transcript variant 1 |
CNY 7,312.00 |
