United States
Germany
Japan
United Kingdom
China
ALPK2 (NM_052947) Human Untagged Clone
CAT＃: SC323571
ALPK2 (untagged)-Kinase deficient mutant (K1718M) of Human alpha-kinase 2 (ALPK2)
CNY 13,000.00
货期*
现货
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HAK |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_052947, the custom clone sequence may differ by one or more nucleotides
ATGAAAGACTCCGAAGGGCCCCAGAGGCCCCCGCTGTGTTTTTTATCTACATTGCTTTCCCAGAAGGTTC CTGAGAAGTCAGACGCTGTGCTTCGCTGCATAATATCTGGTCAGCCCAAGCCAGAGGTAACTTGGTATAA GAATGGTCAGGCCATCGATGGGAGTGGCATTATTTCCAACTATGAATTCTTTGAGAATCAGTATATTCAT GTGTTACATCTCTCTTGCTGTACCAAAAATGATGCTGCTGTCTATCAAATCTCGGCTAAAAACTCTTTTG GAATGATCTGTTGTTCTGCTTCCGTTGAGGTTGAGTGCTCATCAGAGAACCCACAATTGTCTCCTAACCT GGAAGATGACAGGGACAGGGGTTGGAAACATGAAACAGGGACACATGAAGAAGAAAGGGCAAATCAGATT GATGAGAAGGAACATCCTTATAAGGAAGAAGAAAGCATCTCCCCGGGCACTCCCAGGTCAGCTGACTCCT CCCCCTCCAAATCCAACCATTCACTCTCCCTCCAGTCATTGGGCAATCTTGACATTAGTGTGTCCAGTTC TGAAAATCCTTTGGGTGTTAAAGGAACAAGGCACACTGGAGAGGCTTATGATCCAAGTAACACAGAAGAA ATTGCAAATGGGTTGCTTTTTCTTAATTCAAGTCATATTTATGAAAAACAAGACAGATGTTGCCACAAGA CAGTGCATTCCATGGCATCAAAGTTCACGGATGGTGACCTGAACAATGATGGTCCTCATGATGAAGGCTT ACGCTCTAGTCAGCAAAATCCCAAAGTACAGAAATACATTAGCTTCAGCCTCCCGCTATCTGAGGCAACT GCACACATTTACCCAGGTGACAGTGCCGTGGCCAACAAACAACCCAGCCCACAGCTTTCCAGTGAAGACT CTGACAGTGACTATGAACTTTGCCCAGAGATAACCCTAACCTACACCGAGGAGTTTTCAGATGATGACCT GGAGTATCTGGAATGTTCTGATGTTATGACGGATTACTCTAATGCAGTTTGGCAAAGGAACCTGCTGGGG ACTGAGCATGTTTTTTTATTAGAAAGCGATGACGAAGAGATGGAATTCGGTGAGCATTGCCTGGGTGGGT GTGAGCATTTCCTCAGTGGAATGGGTTGTGGGTCTCGGGTGTCGGGTGACGCTGGGCCTATGGTTGCCAC TGCTGGCTTCTGTGGTCATCACTCACAACCCCAAGAAGTTGGGGTGAGGAGCAGCAGAGTCTCCAAGCAC GGTCCCTCATCCCCACAAACAGGGATGACTCTCATTTTGGGACCTCACCAGGATGGAACGTCTTCAGTGA CAGAACAGGGGAGATATAAACTCCCCACTGCTCCCGAGGCTGCTGAAAATGATTATCCAGGAATTCAAGG AGAAACCAGAGACAGCCACCAAGCAAGAGAGGAATTTGCCAGTGACAATCTGCTCAACATGGATGAATCA GTAAGAGAGACAGAGATGAAGCTCTTGTCTGGTGAGTCAGAAAACTCAGGGATGAGCCAGTGTTGGGAGA CGGCAGCTGACAAGAGAGTGGGGGGAAAGGACTTATGGAGCAAGAGGGGTTCAAGGAAATCTGCCAGGGT GAGGCAGCCGGGAATGAAGGGAAATCCCAAGAAGCCGAATGCCAACCTGAGAGAAAGTACAACAGAAGGT ACCCTTCATCTCTGCTCTGCCAAAGAATCTGCTGAGCCCCCACTAACCCAGAGTGATAAAAGAGAGACTT CTCACACCACAGCAGCAGCGACTGGTCGGAGTTCCCATGCTGATGCAAGAGAATGTGCTATTTCAACCCA GGCAGAGCAAGAAGCAAAAACCCTTCAAACTTCAACAGACTCAGTCTCCAAAGAAGGCAACACAAATTGC AAGGGAGAAGGCATGCAAGTTAATACTCTATTTGAAACAAGCCAGGTTCCAGACTGGAGTGATCCTCCTC AGGTACAAGTTCAGGAAACAGTCAGAGAGACAATCTCTTGCAGCCAGATGCCAGCTTTCTCAGAGCCTGC TGGGGAGGAGTCCCCATTCACTGGGACCACAACAATTTCCTTCTCAAACTTAGGAGGGGTCCACAAGGAA AATGCATCATTAGCTCAACACTCGGAGGTCAAACCCTGTACCTGTGGTCCACAGCATGAAGAAAAACAAG ACAGAGATGGCAACATACCTGACAATTTCAGGGAAGACCTAAAATATGAGCAGAGCATCTCAGAAGCCAA TGATGAGACTATGTCCCCAGGTGTGTTCTCAAGGCATCTCCCCAAGGATGCTCGTGCTGACTTCAGGGAG CCTGTGGCTGTCTCTGTTGCTTCCCCTGAACCCACAGATACTGCCCTCACCCTGGAAAATGTGTGTGATG AGCCAAGGGACAGAGAAGCAGTGTGTGCAATGGAGTGTTTTGAGGCTGGTGACCAAGGAACGTGTTTTGA TACCATAGATTCTCTTGTTGGGAGACCAGTTGATAAATATTCGCCTCAAGAAATTTGCTCTGTAGATACG GAACTGGCAGAAGGTCAAAACAAAGTATCTGATTTATGTTCTTCTAATGACAAGACACTGGAAGTCTTTT TTCAGACACAAGTGTCTGAGACTTCAGTGTCTACGTGCAAAAGCAGCAAGGACGGCAACTCAGTCATGTC CCCTCTTTTTACCAGTACTTTCACCTTGAACATTTCACACACAGCTAGTGAAGGTGCCACAGGAGAAAAT CTAGCCAAGGTGGAGAATTCCACCTACCCACTGGCCTCCACAGTACATGCTGGCCAGGAGCAGCCAAGCC CCAGCAACTCAGGAGGGCTTGATGAAACACAGCTCCTTTCTTCTGAGAACAATCCTTTAGTGCAATTTAA AGAAGGAGGTGACAAGAGCCCCAGTCCTAGTGCCGCAGACACCACAGCCACACCAGCCAGTTATAGTTCA ATTGTGAGTTTTCCTTGGGAGAAGCCAACAACATTAACTGCTAATAATGAGTGCTTTCAAGCGACCAGGG AGACTGAGGACACATCAACTGTTACCATTGCCACCGAAGTCCACCCAGCCAAATACCTTGCTGTGTCAAT TCCTGAGGACAAGCATGCAGGTGGCACTGAGGAGAGGTTCCCTCGTGCATCCCATGAAAAGGTTTCCCAA TTTCCTTCCCAAGTGCAGTTGGATCATATTTTAAGTGGTGCTACCATCAAATCTACAAAAGAGCTACTTT GCAGGGCACCCAGTGTGCCAGGAGTCCCACACCATGTCCTGCAGCTCCCAGAGGGAGAGGGTTTCTGCAG TAATTCCCCTCTTCAGGTTGATAACCTGTCTGGAGATAAGAGCCAGACTGTGGACAGAGCAGACTTTAGG AGCTATGAAGAGAATTTCCAAGAAAGAGGAAGTGAAACAAAGCAGGGGGTCCAGCAGCAGAGCCTGTCCC AGCAGGGTTCTCTTTCTGCACCTGATTTCCAACAAAGTTTGCCTACGACATCTGCTGCACAAGAGGAAAG AAACTTGGTGCCCACGGCCCACTCACCCGCAAGCTCTAGGGAAGGAGCAGGGCAGCGCTCAGGTTGGGGG ACGAGGGTCTCCGTGGTGGCTGAAACTGCTGGGGAAGAAGACAGTCAGGCTCTGAGCAACGTTCCATCTC TCTCTGATATCCTTTTGGAAGAGTCTAAAGAATATAGACCTGGAAATTGGGAGGCAGGCAACAAGCTGAA GATTATAACTCTAGAGGCTTCCGCTTCTGAAATCTGGCCACCACGACAACTGACAAATTCTGAGAGCAAG GCATCAGACGGTGGTCTCATAATTCCTGACAAGGTCTGGGCTGTACCTGATAGTCTAAAGGCAGATGCTG TTGTGCCTGAATTGGCCCCCTCTGAAATAGCAGCATTGGCTCACAGTCCAGAGGATGCTGAGTCAGCCCT TGCTGATAGCAGAGAAAGCCATAAAGGCGAAGAGCCCACCATCAGTGTACATTGGAGAAGTCTTTCTTCC CGGGGTTTCAGCCAACCCAGACTCCTGGAGTCATCCGTGGACCCTGTAGATGAAAAGGAGTTATCTGTCA CAGATTCACTGTCAGCGGCTTCTGAAACTGGAGGGAAGGAAAATGTTAACAATGTGAGTCAAGACCAGGA GGAAAAACAACTCAAGATGGATCACACTGCCTTCTTTAAAAAGTTTCTGACCTGCCCTAAAATCCTAGAG TCCTCTGTAGATCCCATTGATGAGATAAGTGTGATAGAGTACACCAGGGCTGGAAAACCAGAGCCCTCTG AAACCACACCACAGGGCGCCAGAGAAGGGGGTCAATCAAATGACGGAAACATGGGCCACGAAGCGGAAAT CCAGCCGGCCATTTTGCAAGTTCCATGTCTCCAGGGAACCATTCTGAGTGAAAATAGAATCAGCAGAAGC CAAGAAGGCAGTATGAAGCAGGAGGCTGAACAAATTCAACCTGAGGAGGCAAAAACTGCCATTTGGCAAG TCCTGCAACCCAGCGAAGGCGGTGAAAGAATTCCAAGTGGATGTAGCATAGGCCAAATACAAGAAAGCAG TGATGGGAGCTTAGGGGAGGCTGAGCAAAGCAAAAAGGACAAAGCAGAATTGATTTCCCCCACTTCACCT CTTTCTAGTTGTCTTCCAATAATGACTCACGCTTCTCTTGGGGTTGACACGCACAACTCCACAGGCCAAA TTCATGACGTCCCTGAAAATGACATAGTTGAGCCCAGAAAGCGTCAGTATGTGTTTCCTGTTTCACAGAA AAGGGGAACTATTGAGAATGAGCGTGGGAAACCTTTGCCCTCTTCTCCTGATCTTACCAGGTTCCCTTGT ACTTCATCTCCTGAAGGAAATGTCACAGACTTTTTGATAAGCCACAAAATGGAGGAACCTAAAATAGAGG TGCTTCAAATTGGGGAAACCAAACCCCCAAGCTCATCTAGCTCCTCAGCGAAGACCTTGGCATTTATTTC AGGAGAACGTGAGTTAGAGAAAGCCCCTAAATTACTGCAGGATCCATGTCAAAAGGGCACCCTGGGCTGT GCGAAAAAGTCCAGGGAGAGAGAGAAGTCCCTGGAAGCCCGAGCAGGCAAATCGCCAGGGACCCTCACAG CAGTGACGGGGTCAGAGGAGGTCAAGAGGAAGCCAGAAGCCCCAGGCAGTGGACATTTAGCTGAGGGAGT AAAGAAGAAAATTTTGTCCAGGGTGGCAGCACTGAGGCTGAAACTGGAAGAAAAGGAAAATATCAGAAAG AACTCAGCCTTTCTTAAAAAGATGCCCAAACTCGAAACATCATTATCACACACAGAAGAGAAACAAGACC CAAAAAAGCCATCTTGCAAAAGAGAAGGAAGAGCTCCAGTATTACTGAAAAAGATCCAAGCTGAGATGTT CCCTGAACACTCTGGAAATGTAAAATTAAGCTGCCAATTTGCAGAAATTCATGAAGATTCTACTATCTGC TGGACAAAAGATTCAAAGTCCATAGCCCAAGTGCAGAGAAGTGCAGGGGACAACTCCACTGTTTCCTTTG CCATCGTGCAAGCCAGTCCGAAGGACCAGGGACTCTATTACTGCTGCATCAAGAACAGCTACGGAAAAGT GACTGCTGAATTTAACCTCACAGCTGAAGTTCTCAAACAGCTGTCAAGTCGCCAGGATACTAAAGGATGT GAAGAGATTGAATTCAGCCAACTCATCTTCAAAGAAGACTTCCTCCATGACAGCTACTTTGGGGGCCGCC TGCGTGGTCAGATCGCCACGGAGGAGCTGCACTTTGGAGAAGGGGTTCACCGCAAAGCCTTCCGCAGCAC AGTGATGCACGGCCTCATGCCTGTCTTCAAACCTGGCCATGCCTGTGTGCTTAAGGTGCACAATGCCATT GCCTATGGGACCAGAAATAATGATGAGCTCATCCAAAGGAACTACAAACTCGCTGCCCAGGAATGCTATG TTCAAAATACTGCCAGGTATTATGCCAAGATCTACGCTGCTGAAGCACAGCCTCTGGAAGGCTTTGGAGA AGTACCTGAGATCATTCCTATTTTTCTTATCCATCGGCCTGAGAACAATATCCCGTATGCTACAGTGGAG GAGGAGCTGATTGGAGAATTTGTGAAGTATTCCATCAGGGATGGGAAAGAAATAAACTTCTTGAGAAGAG AATCAGAAGCTGGTCAGAAATGTTGCACCTTCCAGCACTGGGTGTACCAGAAAACAAGTGGCTGCCTCCT GGTGACGGACATGCAAGGTGTAGGAATGAAGCTAACTGACGTTGGCATAGCAACGCTGGCTAAAGGGTAC AAGGGATTTAAAGGCAACTGTTCCATGACCTTCATTGATCAGTTTAAAGCACTACACCAGTGTAACAAGT ATTGCAAAATGCTGGGACTGAAATCCCTTCAAAACAACAACCAGAAACAGAAGCAGCCGAGCATTGGGAA AAGCAAAGTTCAAACAAACTCTATGACAATAAAGAAGGCAGGGCCTGAGACCCCAGGCGAAAAGAAAACC TAA >OriGene 5' read for mutant NM_052947 unedited
ACGGCCCCGTTGAAGCAATGGGCGGTAGGCGTGTACGGGGGGAGGTCTATATAAGCAGAGCTCGTTTAGT GAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCCGGGAATGAA GGGAAATCCCAAGAAGCCGAATGCCAACCTGAGAGAAAGTACAACAGAAGGTACCCTTCATCTCTGCTCT GCCAAAGAATCTGCTGAGCCCCCACTAACCCAGAGTGATAAAAGAGAGACTTCTCACACCACAGCAGCAG CGACTGGTCGGAGTTCCCATGCTGATGCAAAGAGAATGTGCTATTTCAACCCAGGCAGAGCAAGAGGCAA AAACCCTTCAAACTTTCAACAGACTCCAGTCCTCAAAAAGGGCACCACAAATTGCAGGGAAAAAGGCATG CAAGTTATTCCTCTATTGGAAACAAGCCAGGTTCCAGACGGAATGGATCCTCCTCAGGTACAGGTTCAGG AACAGTTCAAAAAAACAATCTCTTGCAGCCGGATGCCACCTTTTCAAAACCCTGGTGGGGAGAATTCCCC TTTCATGGAACCCAACAATTTCCTTTCTAAACTTAGAAAGGGGCCCACGGAAAAATGCTATATACCTCAC ACTCAGGGTCACACTGTGCCCGTTGGGCCCAGATGTGAAAATCACACACAGAGTGGCATACCCGGTAAAT TTCGGGGAACACTATATGTGCGCAGAGCCTCTAGACCCTATGAGACACTGTCCCCCCGTGTGTCTAGCGC TCTCACAAAGCTCTCTGATCATGAGAACTTGTGTCTCTTGTTCCCGGAACCAAATAGCTGCCTTCTGAGA TGTGTAGACACGAGTCACACCGTGCTCATGTGTGTATGCGTACAGACAGGTGTTACTCACATCCTGTGAC CTTAGATATCCCACATCTCCTAGGCATTGCAGAAGGG >SC323571 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TCRGCAGRCTGACTTTGGAGAGGGGTTCACCGCAAGCCTTCCGCAGCACAGTGATGCACGGCCTCATGCC TGTCTTCAAACCTGGCCATGCCTGTGTGCTTATGGTGCACAATGCCATTGCCTATGGGACCAGAAATAAT GATGAGCTCATCCAAAGGAACTACAAACTCGCTGCCCAGGAATGCTATGTTCAAAATACTGCCAGGTATT ATGCCAAGATCTACGCTGCTGAAGCACAGCCTCTGGAAGGCTTTG |
| Restriction Sites | Please inquire |
| ACCN | NM_052947 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_052947.2, NP_443179.2 |
| RefSeq Size | 6496 bp |
| RefSeq ORF | 5802 bp |
| Locus ID | 115701 |
| UniProt ID | Q86TB3 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | Kinase that recognizes phosphorylation sites in which the surrounding peptides have an alpha-helical conformation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215838 | ALPK2 (Myc-DDK-tagged)-Human alpha-kinase 2 (ALPK2) |
CNY 21,660.00 |
|
| RC215838L1 | Lenti ORF clone of Human alpha-kinase 2 (ALPK2), Myc-DDK-tagged |
CNY 23,560.00 |
|
| RG215838 | ALPK2 (tGFP-tagged) - Human alpha-kinase 2 (ALPK2) |
CNY 23,850.00 |
|
| SC309406 | ALPK2 (untagged)-Human alpha-kinase 2 (ALPK2) |
CNY 41,900.00 |
|
| SC318712 | ALPK2 (untagged)-Human alpha-kinase 2 (ALPK2) |
CNY 41,900.00 |
Customer
Reviews
Loading...
