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Mps1 (TTK) (NM_003318) Human Untagged Clone
CAT＃: SC323540
TTK (untagged)-Kinase deficient mutant (K553M) of Human TTK protein kinase (TTK)
CNY 6,288.00
CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CT96; ESK; MPH1; MPS1; MPS1L1; PYT |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323540 sequence for NM_003318 edited (data generated by NextGen Sequencing)
ATGGAATCCGAGGATTTAAGTGGCAGAGAATTGACAATTGATTCCATAATGAACAAAGTG AGAGACATTAAAAATAAGTTTAAAAATGAAGACCTTACTGATGAACTAAGCTTGAATAAA ATTTCTGCTGATACTACAGATAACTCGGGAACTGTTAACCAAATTATGATGATGGCAAAC AACCCAGAGGACTGGTTGAGTTTGTTGCTCAAACTAGAGAAAAACAGTGTTCCGCTAAGT GATGCTCTTTTAAATAAATTGATTGGTCGTTACAGTCAAGCAATTGAAGCGCTTCCCCCA GATAAATATGGCCAAAATGAGAGTTTTGCTAGAATTCAAGTGAGATTTGCTGAATTAAAA GCTATTCAAGAGCCAGATGATGCACGTGACTACTTTCAAATGGCCAGAGCAAACTGCAAG AAATTTGCTTTTGTTCATATATCTTTTGCACAATTTGAACTGTCACAAGGTAATGTCAAA AAAAGTAAACAACTTCTTCAAAAAGCTGTAGAACGTGGAGCAGTACCACTAGAAATGCTG GAAATTGCCCTGCGGAATTTAAACCTCCAAAAAAAGCAGCTGCTTTCAGAGGAGGAAAAG AAGAATTTATCAGCATCTACGGTATTAACTGCCCAAGAATCATTTTCCGGTTCACTTGGG CATTTACAGAATAGGAACAACAGTTGTGATTCCAGAGGACAGACTACTAAAGCCAGGTTT TTATATGGAGAGAACATGCCACCACAAGATGCAGAAATAGGTTACCGGAATTCATTGAGA CAAACTAACAAAACTAAACAGTCATGCCCATTTGGAAGAGTCCCAGTTAACCTTCTAAAT AGCCCAGATTGTGATGTGAAGACAGATGATTCAGTTGTACCTTGTTTTATGAAAAGACAA ACCTCTAGATCAGAATGCCGAGATTTGGTTGTGCCTGGATCTAAACCAAGTGGAAATGAT TCCTGTGAATTAAGAAATTTAAAGTCTGTTCAAAATAGTCATTTCAAGGAACCTCTGGTG TCAGATGAAAAGAGTTCTGAACTTATTATTACTGATTCAATAACCCTGAAGAATAAAACG GAATCAAGTCTTCTAGCTAAATTAGAAGAAACTAAAGAGTATCAAGAACCAGAGGTTCCA GAGAGTAACCAGAAACAGTGGCAATCTAAGAGAAAGTCAGAGTGTATTAACCAGAATCCT GCTGCATCTTCAAATCACTGGCAGATTCCGGAGTTAGCCCGAAAAGTTAATACAGAGCAG AAACATACCACTTTTGAGCAACCTGTCTTTTCAGTTTCAAAACAGTCACCACCAATATCA ACATCTAAATGGTTTGACCCAAAATCTATTTGTAAGACACCAAGCAGCAATACCTTGGAT GATTACATGAGCTGTTTTAGAACTCCAGTTGTAAAGAATGACTTTCCACCTGCTTGTCAG TTGTCAACACCTTATGGCCAACCTGCCTGTTTCCAGCAGCAACAGCATCAAATACTTGCC ACTCCACTTCAAAATTTACAGGTTTTAGCATCTTCTTCAGCAAATGAATGCATTTCGGTT AAAGGAAGAATTTATTCCATATTAAAGCAGATAGGAAGTGGAGGTTCAAGCAAGGTATTT CAGGTGTTAAATGAAAAGAAACAGATATATGCTATAAAATATGTGAACTTAGAAGAAGCA GATAACCAAACTCTTGATAGTTACCGGAACGAAATAGCTTATTTGAATAAACTACAACAA CACAGTGATAAGATCATCCGACTTTATGATTATGAAATCACGGACCAGTACATCTACATG GTAATGGAGTGTGGAAATATTGATCTTAATAGTTGGCTTAAAAAGAAAAAATCCATTGAT CCATGGGAACGCAAGAGTTACTGGAAAAATATGTTAGAGGCAGTTCACACAATCCATCAA CATGGCATTGTTCACAGTGATCTTAAACCAGCTAACTTTCTGATAGTTGATGGAATGCTA AAGCTAATTGATTTTGGGATTGCAAACCAAATGCAACCAGATACAACAAGTGTTGTTAAA GATTCTCAGGTTGGCACAGTTAATTATATGCCACCAGAAGCAATCAAAGATATGTCTTCC TCCAGAGAGAATGGGAAATCTAAGTCAAAGATAAGCCCCAAAAGTGATGTTTGGTCCTTA GGATGTATTTTGTACTATATGACTTACGGGAAAACACCATTTCAGCAGATAATTAATCAG ATTTCTAAATTACATGCCATAATTGATCCTAATCATGAAATTGAATTTCCCGATATTCCA GAGAAAGATCTTCAAGATGTGTTAAAGTGTTGTTTAAAAAGGGACCCAAAACAGAGGATA TCCATTCCTGAGCTCCTGGCTCATCCATATGTTCAAATTCAAACTCATCCAGTTAACCAA ATGGCCAAGGGAACCACTGAAGAAATGAAATATGTTCTGGGCCAACTTGTTGGTCTGAAT TCTCCTAACTCCATTTTGAAAGCTGCTAAAACTTTATATGAACACTATAGTGGTGGTGAA AGTCATAATTCTTCATCCTCCAAGACTTTTGAAAAAAAAAGGGGAAAAAAATGA Clone variation with respect to NM_003318.4 >OriGene 5' read for mutant NM_003318 unedited
ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGATAGCGACGGGGA AATTCAAACGTGTTTGCGGAAAGGAGTTTGGGTTCCATCTTTTCATTTCCCCAGCGCAGCTTTCTGTAGT TTTTTTCTTAGAAATGGAATCCGAGGATTTAAGTGGCAGAGAATTGACAATTGATTCCATAATGAACAAA GTGAGAGACATTAAAAATAAGTTTAAAAATGAAGACCTTACTGATGAACTAAGCTTGAATAAAATTTCTG CTGATACTACAGATAACTCGGGAACTGTTAACCAAATTATGATGATGGCAAACAACCCAGAGGACTGTTG AGTTTGTTGCCTAAACTAAAAAAAACGTTGTTCCGCTAGTGATGCTTCTTTTAATAAATTGATGGTCGTA CAGTCAAGCATTGAACCGCTCCCCCGATAATTTGGCCAAATGAAATTTTGCTGATTACATTGAATTGCCT GATTAAACCATCCAGAACCCGATATATGACTTGCACTCTTTCAATGCCCAGCCAACTGGAGAATTTGGCT TTGTTATATCCTGGCAATTACTCGCCAAGTGATGTCAAAAGAGTACACTCTCTCAAGCCTTAAACGTGCA CATACCAAAGTGGGAATGCCGCTGGATATAACTCAAACACGTCTTCAAAAGAAAAAAAAATAACACTCCG GTATGCGCAGACTTCGGATATGGGTCTCAGAGGAACTGTATCAGGACCATCACGGTTATAGGAACGCCCA CTGACTATTTCGTCA >SC323540 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TMTATTAAAGCAGATAGGAKGGAGGTTCAGCAAGGTATTTCAGGTGTTAAATGAAAAGAAACAGATATAT GCTATAATGTATGTGAACTTAGAAGAAGCAGATAACCAAACTCTTGATAGTTACCGGAACGAAATAGCTT ATTTGAATAAACTACAACAACACAGTGATAAGATCATCCGACTTTATGATTATGAAATCACGGACCAGTA CATCTACATGGTAATGGAGTGTGGAAATATTGATCTTAATAGTTG |
| Restriction Sites | Please inquire |
| ACCN | NM_003318 |
| Insert Size | 2900 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003318.3, NP_003309.2 |
| RefSeq Size | 2984 bp |
| RefSeq ORF | 2574 bp |
| Locus ID | 7272 |
| UniProt ID | P33981 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Cell cycle, Oocyte meiosis, TGF-beta signaling pathway, Ubiquitin mediated proteolysis, Wnt signaling pathway |
| Gene Summary | This gene encodes a dual specificity protein kinase with the ability to phosphorylate tyrosine, serine and threonine. Associated with cell proliferation, this protein is essential for chromosome alignment at the centromere during mitosis and is required for centrosome duplication. It has been found to be a critical mitotic checkpoint protein for accurate segregation of chromosomes during mitosis. Tumorigenesis may occur when this protein fails to degrade and produces excess centrosomes resulting in aberrant mitotic spindles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2009] Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200093 | TTK (Myc-DDK-tagged)-Human TTK protein kinase (TTK), transcript variant 1 |
CNY 5,320.00 |
|
| RC200093L1 | Lenti ORF clone of Human TTK protein kinase (TTK), transcript variant 1, Myc-DDK-tagged |
CNY 8,680.00 |
|
| RC200093L2 | Lenti ORF clone of Human TTK protein kinase (TTK), transcript variant 1, mGFP tagged |
CNY 8,680.00 |
|
| RC200093L3 | Lenti ORF clone of Human TTK protein kinase (TTK), transcript variant 1, Myc-DDK-tagged |
CNY 8,680.00 |
|
| RC200093L4 | Lenti ORF clone of Human TTK protein kinase (TTK), transcript variant 1, mGFP tagged |
CNY 8,680.00 |
|
| RG200093 | TTK (tGFP-tagged) - Human TTK protein kinase (TTK), transcript variant 1 |
CNY 7,880.00 |
|
| SC118032 | TTK (untagged)-Human TTK protein kinase (TTK), transcript variant 1 |
CNY 2,950.00 |
|
| SC323539 | TTK (untagged)-Kinase deficient mutant (K553M) of Human TTK protein kinase (TTK) |
CNY 6,288.00 |
