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MAP4K1 (NM_007181) Human Untagged Clone
CAT＃: SC323528
MAP4K1 (untagged)-Kinase deficient mutant (K46M) of Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2
CNY 6,112.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HPK1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_007181, the custom clone sequence may differ by one or more nucleotides
ATGGACGTCGTGGACCCTGACATTTTCAATAGAGACCCCCGGGACCACTATGACCTGCTACAGCGGCTGG GTGGCGGCACGTATGGGGAAGTCTTTAAGGCTCGAGACAAGGTGTCAGGGGACCTGGTGGCACTGAAGAT GGTGAAGATGGAGCCTGATGATGATGTCTCCACCCTTCAGAAGGAAATCCTCATATTGAAAACTTGCCGG CACGCCAACATCGTGGCCTACCATGGGAGTTATCTCTGGTTGCAGAAACTCTGGATCTGCATGGAATTCT GTGGGGCTGGTTCTCTCCAGGACATCTACCAAGTGACAGGCTCCCTGTCAGAGCTCCAGATTAGCTATGT CTGCCGGGAAGTGCTCCAGGGACTGGCCTATTTGCACTCACAGAAGAAGATACACAGGGACATCAAGGGA GCTAACATCCTCATCAATGATGCTGGGGAGGTCAGATTGGCTGACTTTGGCATCTCGGCCCAGATTGGGG CTACACTGGCCAGACGCCTCTCTTTCATTGGGACACCCTACTGGATGGCTCCGGAAGTGGCAGCTGTGGC CCTGAAGGGAGGATACAATGAGCTGTGTGACATCTGGTCCCTGGGCATCACGGCCATCGAACTGGCCGAG CTACAGCCACCGCTCTTTGATGTGCACCCTCTCAGAGTTCTCTTCCTCATGACCAAGAGTGGCTACCAGC CTCCCCGACTGAAGGAAAAAGGCAAATGGTCGGCTGCCTTCCACAACTTCATCAAAGTCACTCTGACTAA GAGTCCCAAGAAACGACCCAGCGCCACCAAGATGCTCAGTCATCAACTGGTATCCCAGCCTGGGCTGAAT CGAGGCCTGATCCTGGATCTTCTTGACAAACTGAAGAATCCCGGGAAAGGACCCTCCATTGGGGACATTG AGGATGAGGAGCCCGAGCTACCCCCTGCTATCCCTCGGCGGATCAGATCCACCCACCGCTCCAGCTCTCT GGGGATCCCAGATGCAGACTGCTGTCGGCGGCACATGGAGTTCAGGAAGCTCCGAGGAATGGAGACCAGA CCCCCAGCCAACACCGCTCGCCTACAGCCTCCTCGAGACCTCAGGAGCAGCAGCCCCAGGAAGCAACTGT CAGAGTCGTCTGACGATGACTATGACGACGTGGACATCCCCACCCCTGCAGAGGACACACCTCCTCCACT TCCCCCCAAGCCCAAGTTCCGTTCTCCATCAGACGAGGGTCCTGGGAGCATGGGGGATGATGGGCAGCTG AGCCCGGGGGTGCTGGTCCGGTGTGCCAGTGGGCCCCCACCAAACAGCCCCCGTCCTGGGCCTCCCCCAT CCACCAGCAGCCCCCACCTCACCGCCCATTCAGAACCCTCACTCTGGAACCCACCCTCCCGGGAGCTTGA CAAGCCCCCACTTCTGCCCCCCAAGAAGGAAAAGATGAAGAGAAAGGGATGTGCCCTTCTCGTAAAGTTG TTCAATGGCTGCCCCCTCCGGATCCACAGCACGGCCGCCTGGACACATCCCTCCACCAAGGACCAGCACC TGCTCCTGGGGGCAGAGGAAGGCATCTTCATCCTGAACCGGAATGACCAGGAGGCCACGCTGGAAATGCT CTTTCCTAGCCGGACTACGTGGGTGTACTCCATCAACAACGTTCTCATGTCTCTCTCAGGAAAGACCCCC CACCTGTATTCTCATAGCATCCTTGGCCTGCTGGAACGGAAAGAGACCAGAGCAGGAAACCCCATCGCTC ACATTAGCCCCCACCGCCTACTGGCAAGGAAGAACATGGTTTCCACCAAGATCCAGGACACCAAAGGCTG CCGGGCGTGCTGTGTGGCGGAGGGTGCGAGCTCTGGGGGCCCGTTCCTGTGCGGTGCATTGGAGACGTCC GTTGTCCTGCTTCAGTGGTACCAGCCCATGAACAAATTCCTGCTTGTCCGGCAGGTGCTGTTCCCACTGC CGACGCCTCTGTCCGTGTTCGCGCTGCTGACCGGGCCAGGCTCTGAGCTGCCCGCTGTGTGCATCGGCGT GAGCCCCGGGCGGCCGGGGAAGTCGGTGCTCTTCCACACGGTGCGCTTTGGCGCGCTCTCTTGCTGGCTG GGCGAGATGAGCACCGAGCACAGGGGACCCGTGCAGGTGACCCAGGTAGAGGAAGATATGGTGATGGTGT TGATGGATGGCTCTGTGAAGCTGGTGACCCCGGAGGGGTCCCCAGTCCGGGGACTTCGCACACCTGAGAT CCCCATGACCGAAGCGGTGGAGGCCGTGGCTATGGTTGGAGGTCAGCTTCAGGCCTTCTGGAAGCATGGA GTGCAGGTGTGGGCTCTAGGCTCGGATCAGCTGCTACAGGAGCTGAGAGACCCTACCCTCACTTTCCGTC TGCTTGGCTCCCCCAGGCTGGAGTGCAGTGGCACGATCTCGCCTCACTGCAACCTCCTCCTCCCAGGTTC AAGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCCTGTAG >OriGene 5' read for mutant NM_007181 unedited
ACGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCAGGCCCCCAGGG ATGGACGTCGTGGACCCTGACATTTTCAATAGAGACCCCCGGGACCACTATGACCTGCTACAGCGGCTGG GTGGCGGCACGTATGGGGAAGTCTTTAAGGCTCGAGACAAGGTGTCAGGGGACCTGGTGGCACTGATGAT GGTGAAGATGGAGCCTGATGATGATGTCTCCACCCTTCAGAAGGAAATCCTCATATTGAAAACTTTGCCG GCACGCCAACATCGTGGGCCTACCATGGGAGTTATCTCTGGTTGCAGAAAACTCTGGATCTGCATGGAAT TCTGTGGGGCTGGTTCTCTCCAGACCATCTACCAGTTGACAGGCTCCCTGGTCGAACCTCCGATAACCTA TGTCTGCCGGGAGTTGCTCAGGGACTGCCCTATTTGCCCTACCGAAGAAGAATACAAGGGCCCTCAAGGG ACTTACCTTCCTAATCATTGATGCTGGGAAGGTCAGATTGGCTGACTTGGACTCTCGCCCAAATTGGCTC ACCCTGCCGAACCCCTCTTTTCTTGGGCACCCTATCTGAAGGGCTCGAATGGGCCCTTGGCCCGAGAGGA GAGAACAATAGCCTGTGTGACTGTGTCCGGGGTACAGGACATAAAGGGCACGACTACCCACGCTTGTGTG TGCCCCTAGATCTCTCTCTTACACAATGTGTCAGCGTCGCACATGAAAAGACAAAGTGTCGCTTCCATCT CAGTCTCGCGCAAGGTGCGGAGGACTCGCGAAGCCTAACTGTCACTCGTTAAGCGACTGATCTATGAA >SC323528 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
AKKATGMGCAATGGGCGGTAGSGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCAGGCCCCCAGGGATGG ACGTCGTGGACCCTGACATTTTCAATAGAGACCCCCGGGACCACTATGACCTGCTACAGCGGCTGGGTGG CGGCACGTATGGGGAAGTCTTTAAGGCTCGAGACAAGGTGTCAGG |
| Restriction Sites | Please inquire |
| ACCN | NM_007181 |
| Insert Size | 2900 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007181.3, NP_009112.1 |
| RefSeq Size | 2732 bp |
| RefSeq ORF | 2502 bp |
| Locus ID | 11184 |
| UniProt ID | Q92918 |
| Domains | pkinase, CNH, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | MAPK signaling pathway |
| Gene Summary | Serine/threonine-protein kinase, which may play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. Able to autophosphorylate.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) represents the longer transcript and encodes the longer isoform (2). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220698 | MAP4K1 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2 |
CNY 6,104.00 |
|
| RC220698L1 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2, Myc-DDK-tagged |
CNY 8,504.00 |
|
| RC220698L2 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2, mGFP tagged |
CNY 10,640.00 |
|
| RC220698L3 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2, Myc-DDK-tagged |
CNY 8,504.00 |
|
| RC220698L4 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2, mGFP tagged |
CNY 8,504.00 |
|
| RG220698 | MAP4K1 (tGFP-tagged) - Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2 |
CNY 9,600.00 |
|
| SC310086 | MAP4K1 (untagged)-Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2 |
CNY 16,060.00 |
