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ADCK2 (NM_052853) Human Untagged Clone
CAT＃: SC323527
ADCK2 (untagged)-Kinase deficient mutant (K311M) of Human aarF domain containing kinase 2 (ADCK2)
CNY 4,672.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AARF |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323527 sequence for NM_052853 edited (data generated by NextGen Sequencing)
ATGGTGGCGCCCTGGCGCGTCTCCGTCAGGGTTTGCCTGTCGCACCTGAGGTGCTTCGAG CTCAGACAGGGACTCAGCCTCCTGAGGCCCTCCGAGTGCCCTCGCGATGCCAGGCTCTGC TGGCTTCTGCTGGGCACTTTGCCCAAGGTCGTCTCCCTGTGCGGGGACGTGGGTGAGGGG GCCCCTGACGTTCTGAGTCGGCGAAGGGTCCGCTGCAGCGGGGCGGCTGGCGCGGGGCCC GCGGAGAGCCTCCCCCGAGCGGGACCTCTGGGCGGCGTCTTCCTGCATCTCCGCCTCTGG CTTCGCGCCGGCGCTCTGTTGGTGAAATTCTTCCCCCTCCTACTCCTCTACCCCCTCACC TACCTGGCTCCCAGCGTCTCCACCCTCTGGCTCCACCTGCTTCTGAAAGCCACCGAGACC TCAGGCCCAACCTACATCAAACTGGGCCAGTGGGCCAGCACCCGGCGCGATCTGTTTTCG GAGGCTTTCTGTGCCCAATTTTCCAAGCTGCATGTCCGAGTGACGCCCCACCCGTGGACT CACACTGAGCGCTTCCTTCGGCAGGCTTTTGGGGATGACTGGGGGAGCATCCTCTCTTTT GAGAACCGGGAACCTGTGGGCTCAGGCTGCGTGGCCCAGGTGTACAAAGCATACGCCAAC ACTGCCTTCCTGGAGACTGACAGCGTCCAGAGACTTGGCAGGGCCTCCTGTCTGCCGCCC TTCTCACATACTGGGGCAGTCGGTGGGCTGAGAGAGCTCTTTGGATACCTTGGAAATGGC CGGAAACCTCCAGAAAATCTCGCAGACCAGTCGTTTCTAGAAAGGCTGCTCCTCCCTAAA GCTGACCTGGTTGGATCAAATGCAGGGGTGTCTCGGGCTCAGGTCCCTGGCCACCAACCT GAGGCCACCAACCTCATCTCCGTGGCAGTGATGGTGTTGCACCCTGGCCTGCTCGCTCAG GTGCATATGGACCTGCTGCTGATGAAGATTGGCAGCCGAGTCCTGGGAGTTTTGCCAGGC ATCAAGTGGCTTAGCTTGCCTGAGATTGTGGAGGAATTTGAGAAGCTGATGGTCCAACAG ATTGACCTGCGTTACGAAGCTCAGAATCTAGAACACTTCCAGGTCAACTTCCGGAATGTG AAAGCCGTCAAGTTCCCCACCCCTCTGCGCCCCTTTGTCACCAGAGAAGTCTTGGTGGAA ACGTATGAAGAGAGTGTGCCTGTGTCCAGTTACCAGCAGGCAGGAATTCCCGTGGACTTG AAAAGGAAGATTGCACGGCTGGGGATCAACATGCTCCTGAAGATGATATTTGTGGATAAC TTTGTCCATGCAGACCTTCACCCTGGAAACATCCTGGTTCAGGGTGCCAACGGCCTGTCC TCGAGTCAGGAGGCGCAGCTGCAGCAGGCGGACATCTGTGACACTCTGGTGGTGGCCGTG CCATCTTCCCTCTGCCCGCTGCGACTGGTGCTGCTGGATGCTGGCATTGTGGCGGAGCTG CAGGCCCCTGACCTGAGGAATTTCCGGGCAGTTTTCATGGCTGTGGTGATGGGGCAGGGC CAGAGAGTGGCTGAGCTGATCCTGCATCATGCCCGGGCCAGCGAGTGCAGGGACGTGGAG GGGTTCAAAACCGAGATGGCCATGCTGGTGACCCAGGCCAGGAAGAACACCATCACCCTG GAGAAGCTTCATGTGTCCAGCCTTCTCTCTAGTGTCTTTAAGTTGCTGATGACTCACAAG GTAAAGCTTGAGAGCAACTTTGCCTCCATTGTGTTTGCCATCATGGTGTTGGAGGGGCTT GGCCGCTCACTGGACCCCAAACTGGACATCCTGGAGGCAGCGAGGCCCTTCCTCCTCACG GGCCCAGTGTGCCCCCCGTGA Clone variation with respect to NM_052853.3 932 a=>t;933 a=>g >OriGene 5' read for mutant NM_052853 unedited
CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGCTCTGAAGTGGAGGGCGGGCCGCCTGGGC CGCGGGCCTCGGGAGGATGGTGGCGCCCTGGCGCGTCTCCGTCAGGGTTTGCCTGTCGCACCTGAGGTGC TTCGAGCTCAGACAGGGACTCAGCCTCCTGAGGCCCTCCGAGTGCCCTCGCGATGCCAGGCTCTGCTGGC TTCTGCTGGGCACTTTGCCCAAGGTCGTCTCCCTGTGCGGGGACGTGGGTGAGGGGGCCCCTGACGTTCT GAGTCGGCGAAGGGTCCGCTGCAGCGGGGCGGCTGGCGCGGGGCCGCGGAGAGCCTCCCCCGAGCGGGAC CTCTGGGCGGCGTCTTCCTGCATCTCCGCCTCTGCTTCGCGCGCGCTCTGTTGTGAAATCTCCCCTCTAC TCTCTACCCCCTCACCTACTGCTCAACGTTCTCACCTTCTGCTTCACTGCTTCTGAAAGCACGAAACCTC AGCCAACTAACTCAACTGGCAATGGACCAGCCACCCGGCGCGATATCGGTTTCCGAGAGCTTCTGGCCAA TTACAAGACGTCGACAGTGTCCCAATGTAGACGCCACACCTGACACTCCACTTAGAGCGTTCTCTCTGCA GAGCGCTTTAGGATGAGATCTACGTGGGGAGACTCATCTCTCTTTAGACCGGAACCTGAGCTCTAGCGTC CTGTGCCAAGTCCAAGCTAGCCAACTGCGTCTCGAATCATGAAGTCTCGACTGTAGAGGCCCATGCTGTA CGCCTCTCACTACTGGGAACATGCGTGAGACCTGTGACACTGATAGCGCAACTCTAATCGGCAGCGTCTA GAGGCCTCCCTCACATGAACAGCTATGGATTGA >SC323527 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
GYYCGGGCTAGTCCTGGCMCCACCTGAGGCCACCAACCTCATCTCCGTGGCAGTGATGGTGTTGCACCCT GGCCTGCTCGCTCAGGTGCATATGGACCTGCTGCTGATGAAGATTGGCAGCCGAGTCCTGGGAGTTTTGC CAGGCATCAAGTGGCTTAGCTTGCCTGAGATTGTGGAGGAATTTGAGAAGCTGATGGTCCAACAGATTGA CCTGCGTTACGAAGCTCAGAATCTAGAACACTTCCAGGTCAACTT |
| Restriction Sites | Please inquire |
| ACCN | NM_052853 |
| Insert Size | 2250 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_052853.3, NP_443085.2 |
| RefSeq Size | 2376 bp |
| RefSeq ORF | 1881 bp |
| Locus ID | 90956 |
| UniProt ID | Q7Z695 |
| Domains | ABC1 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | The function of this protein is not yet clear. It is not known if it has protein kinase activity and what type of substrate it would phosphorylate (Ser, Thr or Tyr).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220159 | ADCK2 (Myc-DDK-tagged)-Human aarF domain containing kinase 2 (ADCK2) |
CNY 4,664.00 |
|
| RC220159L1 | Lenti ORF clone of Human aarF domain containing kinase 2 (ADCK2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC220159L2 | Lenti ORF clone of Human aarF domain containing kinase 2 (ADCK2), mGFP tagged |
CNY 5,890.00 |
|
| RC220159L3 | Lenti ORF clone of Human aarF domain containing kinase 2 (ADCK2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC220159L4 | Lenti ORF clone of Human aarF domain containing kinase 2 (ADCK2), mGFP tagged |
CNY 5,890.00 |
|
| RG220159 | ADCK2 (tGFP-tagged) - Human aarF domain containing kinase 2 (ADCK2) |
CNY 4,370.00 |
|
| SC120143 | ADCK2 (untagged)-Human aarF domain containing kinase 2 (ADCK2) |
CNY 4,672.00 |
