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EIF2AK1 (NM_014413) Human Untagged Clone
CAT＃: SC323477
EIF2AK1 (untagged)-Kinase deficient mutant (K210M) of Human eukaryotic translation initiation factor 2-alpha kinase 1 (EIF2AK1), transcript variant 1
CNY 4,696.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HCR; hHRI; HRI; LEMSPAD |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323477 sequence for NM_014413 edited (data generated by NextGen Sequencing)
ATGCAGGGGGGCAACTCCGGGGTCCGCAAGCGCGAAGAGGAGGGCGACGGGGCTGGGGCT GTGGCTGCGCCGCCGGCCATCGACTTTCCCGCCGAGGGCCCGGACCCCGAATATGACGAA TCTGATGTTCCAGCAGAAATCCAGGTGTTAAAAGAACCCCTACAACAGCCAACCTTCCCT TTTGCAGTTGCAAACCAACTCTTGCTGGTTTCTTTGCTGGAGCACTTGAGCCACGTGCAT GAACCAAACCCACTTCGTTCAAGACAGGTGTTTAAGCTACTTTGCCAGACGTTTATCAAA ATGGGGCTGCTGTCTTCTTTCACTTGTAGTGACGAGTTTAGCTCATTGAGACTACATCAC AACAGAGCTATTACTCACTTAATGAGGTCTGCTAAAGAGAGAGTTCGTCAGGATCCTTGT GAGGATATTTCTCGTATCCAGAAAATCAGATCAAGGGAAGTAGCCTTGGAAGCACAAACT TCACGTTACTTAAATGAATTTGAAGAACTTGCCATCTTAGGAAAAGGTGGATACGGAAGA GTATACAAGGTCAGGAATAAATTAGATGGTCAGTATTATGCAATAAAAAAAATCCTGATT AAGGGTGCAACTAAAACAGTTTGCATGATGGTCCTACGGGAAGTGAAGGTGCTGGCAGGT CTTCAGCACCCCAATATTGTTGGCTATCACACCGCGTGGATAGAACATGTTCATGTGATT CAGCCACGAGCAGACAGAGCTGCCATTGAGTTGCCATCTCTGGAAGTGCTCTCCGACCAG GAAGAGGACAGAGAGCAATGTGGTGTTAAAAATGATGAAAGTAGCAGCTCATCCATTATC TTTGCTGAGCCCACCCCAGAAAAAGAAAAACGCTTTGGAGAATCTGACACTGAAAATCAG AATAACAAGTCGGTGAAGTACACCACCAATTTAGTCATAAGAGAATCTGGTGAACTTGAG TCGACCCTGGAGCTCCAGGAAAATGGCTTGGCTGGTTTGTCTGCCAGTTCAATTGTGGAA CAGCAGCTGCCACTCAGGCGTAATTCCCACCTAGAGGAGAGTTTCACATCCACCGAAGAA TCTTCCGAAGAAAATGTCAACTTTTTGGGTCAGACAGAGGCACAGTACCACCTGATGCTG CACATCCAGATGCAGCTGTGTGAGCTCTCGCTGTGGGATTGGATAGTCGAGAGAAACAAG CGGGGCCGGGAGTATGTGGACGAGTCTGCCTGTCCTTATGTTATGGCCAATGTTGCAACA AAAATTTTTCAAGAATTGGTAGAAGGTGTGTTTTACATACATAACATGGGAATTGTGCAC CGAGATCTGAAGCCAAGAAATATTTTTCTTCATGGCCCTGATCAGCAAGTAAAAATAGGA GACTTTGGTCTGGCCTGCACAGACATCCTACAGAAGAACACAGACTGGACCAACAGAAAC GGGAAGAGAACACCAACACATACGTCCAGAGTGGGTACTTGTCTGTACGCTTCACCCGAA CAGTTGGAAGGATCTGAGTATGATGCCAAGTCAGATATGTACAGCTTGGGTGTGGTCCTG CTAGAGCTCTTTCAGCCGTTTGGAACAGAAATGGAGCGAGCAGAAGTTCTAACAGGTTTA AGAACTGGTCAGTTGCCGGAATCCCTCCGTAAAAGGTGTCCAGTGCAAGCCAAGTATATC CAGCACTTAACGAGAAGGAACTCATCGCAGAGACCATCTGCCATTCAGCTGCTGCAGAGT GAACTTTTCCAAAATTCTGGAAATGTTAACCTCACCCTACAGATGAAGATAATAGAGCAA GAAAAAGAAATTGCAGAACTAAAGAAGCAGCTAAACCTCCTTTCTCAAGACAAAGGGGTG AGGGATGACGGAAAGGATGGGGGCGTGGGATGA Clone variation with respect to NM_014413.3 629 a=>t >OriGene 5' read for mutant NM_014413 unedited
CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCTAGCTGCAGCAT CGGAGTGTGGCAGTGCTGGGCTGGCCGGCGGGCTGGGCTGCGGCCCGCGCGCGGCCGGCGATGCAGGGGG GCAACTCCGGGGTCCGCAAGCGCGAAGAGGAGGGCGACGGGGCTGGGGCTGTGGCTGCGCCGCCGGCCAT CGACTTTCCCGCCGAGGGCCCGGACCCCGAATATGACGAATCTGATGTTCCAGCAGAAATCCAGGTGTGA AAAGAACCCCTAACAACAGCCAACCTTCCCTTTTGCAGTTGCAAACCACTCTTGCTGTTTTCTTTGCTGG GAGCACTTGAGCCACGTGCATGACCAAACCCACTTTCGTTTCAGACAGGGGTTTAAAGCTACTTTGCAAG ACGTTTTTCAAAATGGGGCTGGCGGTCCTCTTCACTTTAGGGCCGAGTTTAGCCTATTGGACCCACTACA CACGAACCTTACTACCTAATGAGGTCGGCTAAGAGAAGTCGTCGGATCTGTGAGATTCCCGATCGGAATC CATCAGGAATACCTGGACCAACTTCGTTCTAAGAATGGAACTGCTCTGGGGAGGTGACGGGAGATCCTCC GAAATTAGGCGTATAGCCTAGAACCGTAGTGGCAAACGTTGCAAAGTCCACGAACTTTCGGGCTTTCACA AAAATGTCAAACGACGACGGATCGTATAACCGAGCGCTTAC >SC323477 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TAYYMTCRGGAAWAATTAGTGWCAGTATTATGCAATAAAWAAAATCCTGATTAAGGGTGCAACTAAAACA GTTTGCATGATGGTCCTACGGGAAGTGAAGGGKGCAACTAAAACAGTTTGCATGATGGTCCTACGGGAAG TGAAGAATCCTGATTAAGGGTGCAACTAAAACAGTTTGCATGATGGTCCTACGGGAAGTGAAGAATCCTG ATTAAGGGTGCAACTAAAACAGTTTGCATGATGGTCCTACGGGAA |
| Restriction Sites | Please inquire |
| ACCN | NM_014413 |
| Insert Size | 2240 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014413.2, NP_055228.2 |
| RefSeq Size | 2813 bp |
| RefSeq ORF | 1893 bp |
| Locus ID | 27102 |
| UniProt ID | Q9BQI3 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200065 | EIF2AK1 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2-alpha kinase 1 (EIF2AK1), transcript variant 1 |
CNY 4,688.00 |
|
| RC200065L1 | Lenti ORF clone of Human eukaryotic translation initiation factor 2-alpha kinase 1 (EIF2AK1), transcript variant 1, Myc-DDK-tagged |
CNY 8,170.00 |
|
| RC200065L2 | Lenti ORF clone of Human eukaryotic translation initiation factor 2-alpha kinase 1 (EIF2AK1), transcript variant 1, mGFP tagged |
CNY 7,088.00 |
|
| RC200065L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 2-alpha kinase 1 (EIF2AK1), transcript variant 1, Myc-DDK-tagged |
CNY 8,170.00 |
|
| RC200065L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 2-alpha kinase 1 (EIF2AK1), transcript variant 1, mGFP tagged |
CNY 8,170.00 |
|
| RG200065 | EIF2AK1 (tGFP-tagged) - Human eukaryotic translation initiation factor 2-alpha kinase 1 (EIF2AK1), transcript variant 1 |
CNY 6,288.00 |
|
| SC115013 | EIF2AK1 (untagged)-Human eukaryotic translation initiation factor 2-alpha kinase 1 (EIF2AK1), transcript variant 1 |
CNY 2,950.00 |
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