United States
Germany
Japan
United Kingdom
China
ACVRL1 (NM_000020) Human Untagged Clone
CAT＃: SC323433
ACVRL1 (untagged)-Kinase deficient mutant (K229M) of Human activin A receptor type II-like 1 (ACVRL1), transcript variant 1
CNY 3,752.00
CNY 8,080.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ACVRLK1; ALK-1; ALK1; HHT; HHT2; ORW2; SKR3; TSR-I |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323433 sequence for NM_000020 edited (data generated by NextGen Sequencing)
ATGACCTTGGGCTCCCCCAGGAAAGGCCTTCTGATGCTGCTGATGGCCTTGGTGACCCAG GGAGACCCTGTGAAGCCGTCTCGGGGCCCGCTGGTGACCTGCACGTGTGAGAGCCCACAT TGCAAGGGGCCTACCTGCCGGGGGGCCTGGTGCACAGTAGTGCTGGTGCGGGAGGAGGGG AGGCACCCCCAGGAACATCGGGGCTGCGGGAACTTGCACAGGGAGCTCTGCAGGGGGCGC CCCACCGAGTTCGTCAACCACTACTGCTGCGACAGCCACCTCTGCAACCACAACGTGTCC CTGGTGCTGGAGGCCACCCAACCTCCTTCGGAGCAGCCGGGAACAGATGGCCAGCTGGCC CTGATCCTGGGCCCCGTGCTGGCCTTGCTGGCCCTGGTGGCCCTGGGTGTCCTGGGCCTG TGGCATGTCCGACGGAGGCAGGAGAAGCAGCGTGGCCTGCACAGCGAGCTGGGAGAGTCC AGTCTCATCCTGAAAGCATCTGAGCAGGGCGACAGCATGTTGGGGGACCTCCTGGACAGT GACTGCACCACAGGGAGTGGCTCAGGGCTCCCCTTCCTGGTGCAGAGGACAGTGGCACGG CAGGTTGCCTTGGTGGAGTGTGTGGGAAAAGGCCGCTATGGCGAAGTGTGGCGGGGCTTG TGGCACGGTGAGAGTGTGGCCGTCATGATCTTCTCCTCGAGGGATGAACAGTCCTGGTTC CGGGAGACTGAGATCTATAACACAGTGTTGCTCAGACACGACAACATCCTAGGCTTCATC GCCTCAGACATGACCTCCCGCAACTCGAGCACGCAGCTGTGGCTCATCACGCACTACCAC GAGCACGGCTCCCTCTACGACTTTCTGCAGAGACAGACGCTGGAGCCCCATCTGGCTCTG AGGCTAGCTGTGTCCGCGGCATGCGGCCTGGCGCACCTGCACGTGGAGATCTTCGGTACA CAGGGCAAACCAGCCATTGCCCACCGCGACTTCAAGAGCCGCAATGTGCTGGTCAAGAGC AACCTGCAGTGTTGCATCGCCGACCTGGGCCTGGCTGTGATGCACTCACAGGGCAGCGAT TACCTGGACATCGGCAACAACCCGAGAGTGGGCACCAAGCGGTACATGGCACCCGAGGTG CTGGACGAGCAGATCCGCACGGACTGCTTTGAGTCCTACAAGTGGACTGACATCTGGGCC TTTGGCCTGGTGCTGTGGGAGATTGCCCGCCGGACCATCGTGAATGGCATCGTGGAGGAC TATAGACCACCCTTCTATGATGTGGTGCCCAATGACCCCAGCTTTGAGGACATGAAGAAG GTGGTGTGTGTGGATCAGCAGACCCCCACCATCCCTAACCGGCTGGCTGCAGACCCGGTC CTCTCAGGCCTAGCTCAGATGATGCGGGAGTGCTGGTACCCAAACCCCTCTGCCCGACTC ACCGCGCTGCGGATCAAGAAGACACTACAAAAAATTAGCAACAGTCCAGAGAAGCCTAAA GTGATTCAATAG Clone variation with respect to NM_000020.2 686 a=>t >OriGene 5' read for mutant NM_000020 unedited
ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCATTTAGGTGA CACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGAGGAAACGGTTTA TTAGGAGGGAGTGGTGGAGCTGGGCCAGGCAGGAAGACGCTGGAATAAGAAACATTTTTGCTCCAGCCCC CATCCCAGTCCCGGGAGGCTGCCGCGCCAGCTGCGCCGAGCGAGCCCCTCCCCGGCTCCAGCCCGGTCCG GGGCCGCGCCCGGACCCCAGCCCGCCGTCCAGCGCTGGCGGTGCAACTGCGGGCCGCGCGGTGGAGGGGA GGTGCCCCCGGTCCGCCAAAGGCTACCGCCCCGCCCCCCGCAAAACGGGGCCCAGAGGGACCATGAACCT TGGGCTCCCCCAGAAAGGGCCTTTTGATGCCTGTTAAGGCCTGGGTAACCCAGGGAAACCTGGTAAAGCC GCCTCGGGGCCGATGGGGACTGGAAAGGGAAAAACCCCTCTTTAAGGGGGCTAAATGCCAGG >SC323433 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CGGGCTGGTGATGTGTGGGAAGGCCGCTATGGCGAAGTGTGGCGGGGCTTGTGGCACGGTGAGAGTGTGG CCGTCATGATCTTCTCCTCGAGGGATGAACAGTCCGCTTGTGGCACGGTGAGAGTGTGGCCGTCATGATC TTCTCCTCGAGGGATGAACAGTCCTGGTTCCGGGAGACTGAGATCTATAACACAGTGTTGCTCAGACACG ACAACATCCTAGGCTTCATCGCCTCAGACATGACCTCCCGCAACT |
| Restriction Sites | Please inquire |
| ACCN | NM_000020 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000020.1, NP_000011.1 |
| RefSeq Size | 1970 bp |
| RefSeq ORF | 1512 bp |
| Locus ID | 94 |
| UniProt ID | P37023 |
| Domains | Activin_recp, pkinase, TyrKc, S_TKc, GS |
| Protein Families | Druggable Genome, Protein Kinase, Transmembrane |
| Protein Pathways | Cytokine-cytokine receptor interaction, TGF-beta signaling pathway |
| Gene Summary | This gene encodes a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. The encoded protein, sometimes termed ALK1, shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in this gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same isoform. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206568 | ACVRL1 (Myc-DDK-tagged)-Human activin A receptor type II-like 1 (ACVRL1), transcript variant 1 |
CNY 3,744.00 |
|
| RC206568L1 | Lenti ORF clone of Human activin A receptor type II-like 1 (ACVRL1), transcript variant 1, Myc-DDK-tagged |
CNY 6,144.00 |
|
| RC206568L2 | Lenti ORF clone of Human activin A receptor type II-like 1 (ACVRL1), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RC206568L3 | Lenti ORF clone of Human activin A receptor type II-like 1 (ACVRL1), transcript variant 1, Myc-DDK-tagged |
CNY 6,144.00 |
|
| RC206568L4 | Lenti ORF clone of Human activin A receptor type II-like 1 (ACVRL1), transcript variant 1, mGFP tagged |
CNY 6,144.00 |
|
| RG206568 | ACVRL1 (tGFP-tagged) - Human activin A receptor type II-like 1 (ACVRL1), transcript variant 1 |
CNY 5,040.00 |
|
| SC321860 | ACVRL1 (untagged)-Human activin A receptor type II-like 1 (ACVRL1), transcript variant 1 |
CNY 2,950.00 |
