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SGK2 (NM_170693) Human Untagged Clone
CAT＃: SC323365
SGK2 (untagged)-Kinase deficient mutant (K64M) of Human serum/glucocorticoid regulated kinase 2 (SGK2), transcript variant 1
CNY 3,656.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | dJ138B7.2; H-SGK2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323365 sequence for NM_170693 edited (data generated by NextGen Sequencing)
ATGAACTCTAGCCCAGCTGGGACCCCAAGTCCACAGCCCTCCAGGGCCAATGGGAACATC AACCTGGGGCCTTCAGCCAACCCAAATGCCCAGCCCACGGACTTCGACTTCCTCAAAGTC ATCGGCAAAGGGAACTACGGGAAGGTCCTACTGGCCAAGCGCAAGTCTGATGGGGCGTTC TATGCAGTGATGGTACTACAGAAAAAGTCCATCTTAAAGAAGAAAGAGCAGAGCCACATC ATGGCAGAGCGCAGTGTGCTTCTGAAGAACGTGCGGCACCCCTTCCTCGTGGGCCTGCGC TACTCCTTCCAGACACCTGAGAAGCTCTACTTCGTGCTCGACTATGTCAACGGGGGAGAG CTCTTCTTCCACCTGCAGCGGGAGCGCCGGTTCCTGGAGCCCCGGGCCAGGTTCTACGCT GCTGAGGTGGCCAGCGCCATTGGCTACCTGCACTCCCTCAACATCATTTACAGGGATCTG AAACCAGAGAACATTCTCTTGGACTGCCAGGGACACGTGGTGCTGACGGATTTTGGCCTC TGCAAGGAAGGTGTAGAGCCTGAAGACACCACATCCACATTCTGTGGTACCCCTGAGTAC TTGGCACCTGAAGTGCTTCGGAAAGAGCCTTATGATCGAGCAGTGGACTGGTGGTGCTTG GGGGCAGTCCTCTACGAGATGCTCCATGGCCTGCCGCCCTTCTACAGCCAAGATGTATCC CAGATGTATGAGAACATTCTGCACCAGCCGCTACAGATCCCCGGAGGCCGGACAGTGGCC GCCTGTGACCTCCTGCAAAGCCTTCTCCACAAGGACCAGAGGCAGCGGCTGGGCTCCAAA GCAGACTTTCTTGAGATTAAGAACCATGTATTCTTCAGCCCCATAAACTGGGATGACCTG TACCACAAGAGGCTAACTCCACCCTTCAACCCAAATGTGACAGGACCTGCTGACTTGAAG CATTTTGACCCAGAGTTCACCCAGGAAGCTGTGTCCAAGTCCATTGGCTGTACCCCTGAC ACTGTGGCCAGCAGCTCTGGGGCCTCAAGTGCATTCCTGGGATTTTCTTATGCGCCAGAG GATGATGACATCTTGGATTGCTAG Clone variation with respect to NM_170693.2 191 a=>t >OriGene 5' read for mutant NM_170693 unedited
ACCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGACAAGTCAGGATT GTGCCTCCAGTCAGAAGGACCATGAAGAGAGAGAAGAGGGCAGAGCCGTGCATGGGGCTGCTCCCCAGGA CCTGAGCAGGAACCTGGAGTTTTCAGAGCTGCCTGATCATTGCTACAGAATGAACTCTAGCCCAGCTGGG ACCCCAAGTCCACAGCCCTCCAGGGGCCAATGGGAACATCAACCTGGGGGCCTTCAGCCAACCCAGATGC GCAGCTCCACGGACTTCGACTTTCCTCAAGGTCATCGGCAAAGGGAACTACGGAAAGGTCCACCGGGCGA ACCGCAATTCTGAGGGGGCGGTTCTATGCATGGATGGTCCTACAGAAAAAGTTCAATCTAAAAGAAAAAA AAACCAAAGCCCATTCTTGCCGAAACCGCAGTTGTGCTTTGGAGAAAGTGGGGGGCCCCCCTTCCCTCGG GGCCCGGCCCAATCCTTTCCAAAACCTGGGAAACCCTTACTTTTGGGCGAAAATGTTTACAGGGGGAAAG AGTTTTTTTTCATTGGGAGGGGGCGCCCGGTTTCGGGACCCCGGGCCCAGGTTTCACGCTGCAGGTGGGG CCCGGCCCTTTGTATCTGTGTTCCCCCATCATTTTTACGGGTGTTGGAACCAGAAAATTTTTTTTTACAC GCGAGGCACTGTGGCTGCCGAGTTTTTTTGCCTCTCAGAAGGAGGTTAACCCCCGAACACCCCACCCTCT TTGGGGGCACCCGAGATTTGCGCGCGATGAGTGTCTAGAAGCCACTATATATCCATCTATACGGTGTGCT GTGTGTGCGGCCTTCTACAGAGACATAGTGAGCGGACTCTCTGCACATAGATATCGACGATATGAAATAT ACGCTCTGACGACA >SC323365 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
AYARKSRGRYTTCGCGTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGT TTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGACAAGT CAGGATTGTGCCTCCAGTCAGAAGGACCATGAAGAGAGAGAAGAGGGCAGAGCCGTGCATGGGGCTGCTC CCCAGGACCTGAGCAGGAACCTGGAGTTTTCAGAGCTGCCTGATC |
| Restriction Sites | Please inquire |
| ACCN | NM_170693 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_170693.1, NP_733794.1 |
| RefSeq Size | 1862 bp |
| RefSeq ORF | 1104 bp |
| Locus ID | 10110 |
| UniProt ID | Q9HBY8 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes a serine/threonine protein kinase. Although this gene product is similar to serum- and glucocorticoid-induced protein kinase (SGK), this gene is not induced by serum or glucocorticoids. This gene is induced in response to signals that activate phosphatidylinositol 3-kinase, which is also true for SGK. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010] Transcript Variant: This variant (1) contains an alternate in-frame exon in the 5' coding region, compared to variant 2. Variants 1 and 3 encode the same isoform. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204101 | SGK2 (Myc-DDK-tagged)-Human serum/glucocorticoid regulated kinase 2 (SGK2), transcript variant 1 |
CNY 3,656.00 |
|
| RC204101L1 | Lenti ORF clone of Human serum/glucocorticoid regulated kinase 2 (SGK2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204101L2 | Lenti ORF clone of Human serum/glucocorticoid regulated kinase 2 (SGK2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC204101L3 | Lenti ORF clone of Human serum/glucocorticoid regulated kinase 2 (SGK2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204101L4 | Lenti ORF clone of Human serum/glucocorticoid regulated kinase 2 (SGK2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG204101 | SGK2 (tGFP-tagged) - Human serum/glucocorticoid regulated kinase 2 (SGK2), transcript variant 1 |
CNY 4,370.00 |
|
| SC110120 | SGK2 (untagged)-Human serum/glucocorticoid regulated kinase 2 (SGK2), transcript variant 1 |
CNY 7,220.00 |
|
| SC323599 | SGK2 (untagged)-Kinase deficient mutant (K64M) of Human serum/glucocorticoid regulated kinase 2 (SGK2), transcript variant 1 |
CNY 3,656.00 |
