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DBT (NM_001918) Human Untagged Clone
CAT＃: SC322453
DBT (untagged)-Human dihydrolipoamide branched chain transacylase E2 (DBT), nuclear gene encoding mitochondrial protein
CNY 3,656.00
CNY 7,700.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2; BCOADC-E2; E2; E2B |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for SC322453
ATGGCTGCAGTCCGTATGCTGAGAACCTGGAGCAGGAATGCGGGGAAGCTGATTTGTGTT
CGCTATTTTCAAACATGTGGTAATGTTCATGTTTTGAAGCCAAATTATGTGTGTTTCTTT GGTTATCCTTCATTCAAGTATAGTCATCCACATCACTTCCTGAAAACAACTGCTGCTCTC CGTGGACAGGTTGTTCAGTTCAAGCTCTCAGACATTGGAGAAGGGATTAGAGAAGTAACT GTTAAAGAATGGTATGTAAAAGAAGGAGATACAGTGTCTCAGTTTGATAGCATCTGTGAA GTTCAAAGTGATAAAGCTTCTGTTACCATCACTAGTCGTTATGATGGAGTCATTAAAAAA CTCTATTATAATCTAGACGATATTGCCTATGTGGGGAAGCCATTAGTAGACATAGAAACG GAAGCTTTAAAAGATTCAGAAGAAGATGTTGTTGAAACTCCTGCAGTGTCTCATGATGAA CATACACACCAAGAGATAAAGGGCCGAAAAACACTGGCAACTCCTGCAGTTCGCCGTCTG GCAATGGAAAACAATATTAAGCTGAGTGAAGTTGTTGGCTCAGGAAAAGATGGCAGAATA CTTAAAGAAGATATCCTCAACTATTTGGAAAAGCAGACAGGAGCTATATTGCCTCCTTCA CCCAAAGTTGAAATTATGCCACCTCCACCAAAGCCAAAAGACATGACTGTTCCTATACTA GTATCAAAACCTCCGGTATTCACAGGCAAAGACAAAACAGAACCCATAAAAGGCTTTCAA AAAGCAATGGTCAAGACTATGTCTGCAGCCCTGAAGATACCTCATTTTGGTTATTGTGAT GAGATTGACCTTACTGAACTGGTTAAGCTCCGAGAAGAATTAAAACCCATTGCATTTGCT CGTGGAATTAAACTCTCCTTTATGCCTTTCTTCTTAAAGGCTGCTTCCTTGGGATTACTA CAGTTTCCTATCCTTAACGCTTCTGTGGATGAAAACTGCCAGAATATAACATATAAGGCT TCTCATAACATTGGGATAGCAATGGATACTGAGCAGGGTTTGATTGTCCCTAATGTGAAA AATGTTCAGATCTGCTCTATATTTGACATCGCCACTGAACTGAACCGCCTCCAGAAATTG GGCTCTGTGGGTCAGCTCAGCACCACTGATCTTACAGGAGGAACATTTACTCTTTCCAAC ATTGGATCAATTGGTGGTACCTTTGCCAAACCAGTGATAATGCCACCTGAAGTAGCCATT GGGGCCCTTGGATCAATTAAGGCCATTCCCCGATTTAACCAGAAAGGAGAAGTATATAAG GCACAGATAATGAATGTGAGCTGGTCAGCTGATCACAGAGTTATTGATGGTGCTACAATG TCACGCTTCTCCAATTTGTGGAAATCCTATTTAGAAAACCCAGCTTTTATGCTACTAGAT CTGAAATGAAGACTGATAAGACATTCTTGAACTTTTTGAGCTTCCAAAGAGTATGTAAAC CCTAGCTGTGCCAGCACATGTTCATCTTTACAATTTATATTGTAAATGATTTGTATCGTA TGATTAAGGATCTAAGGCACAATATTTGTCACTGTTCTATTAGACTTTTTACTGAAAATG AATAATGGTGTAATGGTTCTCCTGGGGCTGTCACATTTTATAGGTCAGAGTGTGACTTCT TAATATGGTGCTGATGTTTTTGTGTCAATGGCTTGAAACTGGCAAGATTAACAAAATTAG GCCGGGCATGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCCAGGTGGGCGGAT CACCTGAGGTTAGAAGTTTGAGACCAGCCTGGCCAACATGGTGAAACCTGGCCTCTACTA AAAATACAAAAATTAGCCGGGTGTGGTGGTGGGTACCTATAATCCCAGCTACTTGGGAGG CTGAGGCAGGAGAATCGCTTGAACCTGGGAGGTGGAGGTTGCAGTGAGCTGAGATCGTGC TATTGCACTCCAGCCTGGGCGACAGAGCAAGACGCCATCTCAAAAACAAAAAAACAAAAT TCATGTTACTAAAAGACAGGTAGCCATATACAGACAGTATATGCCCTATTTTTTTTAACT GACTCTTAATGAAACTTTAATTTTACTTAATTAAGAAATGGAATTTATATACAAAAATAT TTTCCATTTCCGTTATTATGCTAATTGTTGTATGAAATAAGTGCAATTATACTTCTCTTT TGAGATATCCAAGAGTATATTCTTGCTCTGTATAGAGAATATCATCTGATAGTGTCTTAT TTATATTAATTAATGTCTTTGAAAAGGGAAAAGTATAAACTGGCCTTAAAATTGTCCAAT TATAGTTTTATAACCAGTCTATTAAAGGTGTTTGTTTAAAATGGAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_001918 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001918.2, NP_001909.2 |
| RefSeq Size | 10831 bp |
| RefSeq ORF | 1449 bp |
| Locus ID | 1629 |
| UniProt ID | P11182 |
| Domains | biotin_lipoyl, 2-oxoacid_dh, e3_binding |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Valine, leucine and isoleucine degradation |
| Gene Summary | The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201998 | DBT (Myc-DDK-tagged)-Human dihydrolipoamide branched chain transacylase E2 (DBT), nuclear gene encoding mitochondrial protein |
CNY 3,656.00 |
|
| RC201998L1 | Lenti ORF clone of Human dihydrolipoamide branched chain transacylase E2 (DBT), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC201998L2 | Lenti ORF clone of Human dihydrolipoamide branched chain transacylase E2 (DBT), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 6,180.00 |
|
| RC201998L3 | Lenti ORF clone of Human dihydrolipoamide branched chain transacylase E2 (DBT), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 6,180.00 |
|
| RC201998L4 | Lenti ORF clone of Human dihydrolipoamide branched chain transacylase E2 (DBT), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 6,180.00 |
|
| RG201998 | DBT (tGFP-tagged) - Human dihydrolipoamide branched chain transacylase E2 (DBT), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
|
| SC118943 | DBT (untagged)-Human dihydrolipoamide branched chain transacylase E2 (DBT), nuclear gene encoding mitochondrial protein |
CNY 2,950.00 |
