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Ribonuclease H2, subunit A (RNASEH2A) (NM_006397) Human Untagged Clone
CAT＃: SC320899
RNASEH2A (untagged)-Human ribonuclease H2, subunit A (RNASEH2A)
CNY 3,600.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AGS4; JUNB; RNASEHI; RNHIA; RNHL; THSD8 |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_006397.2
CCCGCTCCTGCAGTATTAGTTCTTGCAGCTGGTGGTGGCGGCTGAGGCGGCATGGATCTC
AGCGAGCTGGAGAGAGACAATACAGGCCGCTGTCGCCTGAGTTCGCCTGTGCCCGCGGTG TGCCGCAAGGAGCCTTGCGTCCTGGGCGTCGATGAGGCGGGCAGGGGCCCCGTGCTGGGC CCCATGGTCTACGCCATCTGTTATTGTCCCCTGCCTCGCCTGGCAGATCTGGAGGCGCTG AAAGTGGCAGACTCAAAGACCCTATTGGAGAGCGAGCGGGAAAGGCTGTTTGCGAAAATG GAGGACACGGACTTTGTCGGCTGGGCGCTGGATGTGCTGTCTCCAAACCTCATCTCTACC AGCATGCTTGGGCGGGTCAAATACAACCTGAACTCCCTGTCACATGATACAGCCACTGGG CTTATACAGTATGCATTGGACCAGGGCGTGAACGTCACCCAGGTATTCGTGGACACCGTA GGGATGCCAGAGACATACCAGGCGCGGCTGCAGCAAAGTTTTCCCGGGATTGAGGTGACG GTCAAGGCCAAAGCAGATGCCCTCTACCCGGTGGTTAGTGCTGCCAGCATCTGTGCCAAG GTGGCCCGGGACCAGGCCGTGAAGAAATGGCAGTTCGTGGAGAAACTGCAGGACTTGGAT ACTGATTATGGCTCAGGCTACCCCAATGATCCCAAGACAAAAGCGTGGTTGAAGGAGCAC GTGGAGCCTGTGTTCGGCTTCCCCCAGTTTGTCCGGTTCAGCTGGCGCACGGCCCAGACC ATCCTGGAGAAAGAGGCGGAAGATGTTATATGGGAGGACTCAGCATCCGAGAATCAGGAG GGACTCAGGAAGATCACATCCTACTTCCTCAATGAAGGGTCCCAAGCCCGTCCCCGTTCT TCCCACCGATATTTCCTGGAACGCGGCCTGGAGTCAGCAACCAGCCTCTAGCAGCTGCCT CTACGCGCTCTACCTGCTTCCCCAACCCAGACATTAAAATTGTTTAAGGAGACCCCCAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_006397 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006397.2, NP_006388.2 |
| RefSeq Size | 1148 bp |
| RefSeq ORF | 900 bp |
| Locus ID | 10535 |
| UniProt ID | O75792 |
| Domains | RNase_HII |
| Protein Pathways | DNA replication |
| Gene Summary | The protein encoded by this gene is a component of the heterotrimeric type II ribonuclease H enzyme (RNAseH2). RNAseH2 is the major source of ribonuclease H activity in mammalian cells and endonucleolytically cleaves ribonucleotides. It is predicted to remove Okazaki fragment RNA primers during lagging strand DNA synthesis and to excise single ribonucleotides from DNA-DNA duplexes. Mutations in this gene cause Aicardi-Goutieres Syndrome (AGS), a an autosomal recessive neurological disorder characterized by progressive microcephaly and psychomotor retardation, intracranial calcifications, elevated levels of interferon-alpha and white blood cells in the cerebrospinal fluid.[provided by RefSeq, Aug 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204032 | RNASEH2A (Myc-DDK-tagged)-Human ribonuclease H2, subunit A (RNASEH2A) |
CNY 3,600.00 |
|
| RC204032L1 | Lenti ORF clone of Human ribonuclease H2, subunit A (RNASEH2A), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC204032L2 | Lenti ORF clone of Human ribonuclease H2, subunit A (RNASEH2A), mGFP tagged |
CNY 5,890.00 |
|
| RC204032L3 | Lenti ORF clone of Human ribonuclease H2, subunit A (RNASEH2A), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204032L4 | Lenti ORF clone of Human ribonuclease H2, subunit A (RNASEH2A), mGFP tagged |
CNY 5,890.00 |
|
| RG204032 | RNASEH2A (tGFP-tagged) - Human ribonuclease H2, subunit A (RNASEH2A) |
CNY 5,200.00 |
|
| SC116088 | RNASEH2A (untagged)-Human ribonuclease H2, subunit A (RNASEH2A) |
CNY 3,600.00 |
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