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ULK3 (NM_001099436) Human Untagged Clone
CAT＃: SC316533
ULK3 (untagged)-Human unc-51-like kinase 3 (C. elegans) (ULK3)
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC316533 representing NM_001099436.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGGGCCCGGCTGGGGTCCCCCGCGCCTGGACGGCTTCATCCTCACCGAGCGCCTGGGCAGCGGC ACGTACGCCACGGTGTACAAGGCCTACGCCAAGAAGGACACTCGTGAAGTGGTAGCCATAAAGTGTGTA GCCAAGAAAAGTCTGAACAAGGCATCGGTGGAGAACCTCCTCACGGAGATTGAGATCCTCAAGGGCATT CGACATCCCCACATTGTGCAGCTGAAAGACTTTCAGTGGGACAGTGACAATATCTACCTCATCATGGAG TTTTGCGCAGGGGGCGACCTGTCTCGCTTCATCCATACCCGCAGGATTCTGCCTGAGAAGGTGGCGCGT GTCTTCATGCAGCAATTAGCTAGCGCCCTGCAATTCCTGCATGAACGGAATATCTCTCACCTGGATCTG AAGCCACAGAACATTCTACTGAGCTCCTTGGAGAAGCCCCACCTAAAACTGGCAGACTTTGGTTTCGCA CAACACATGTCCCCGTGGGATGAGAAGCACGTGCTCCGTGGCTCCCCCCTCTACATGGCCCCCGAGATG GTGTGCCAGCGGCAGTATGACGCCCGCGTGGACCTCTGGTCCATGGGGGTCATCCTGTATGAAGCCCTC TTCGGGCAGCCCCCCTTTGCCTCCAGGTCGTTCTCGGAGCTGGAAGAGAAGATCCGTAGCAACCGGGTC ATCGAGCTCCCCTTGCGGCCCCTGCTCTCCCGAGACTGCCGGGACCTACTGCAGCGGCTCCTGGAGCGG GACCCCAGCCGTCGCATCTCCTTCCAGGACTTTTTTGCGCACCCCTGGGTGGACCTGGAGCACATGCCC AGTGGGGAGAGTCTGGGGCGAGCAACCGCCCTGGTGGTGCAGGCTGTGAAGAAAGACCAGGAGGGGGAT TCAGCAGCTGCCTTATCACTCTACTGCAAGGCTCTGGACTTCTTTGTACCTGCCCTGCACTATGAAGTG GATGCCCAGCGGAAGGAGGCAATTAAGGCAAAGGTGGGGCAGTACGTGTCCCGGGCTGAGGAGCTCAAG GCCATCGTCTCCTCTTCCAATCAGGCCCTGCTGAGGCAGGGGACCTCTGCCCGAGACCTGCTCAGAGAG ATGGCCCGGGACAAGCCACGCCTCCTAGCTGCCCTGGAAGTGGCTTCAGCTGCCATGGCCAAGGAGGAG GCCGCCGGCGGGGAGCAGGATGCCCTGGACCTGTACCAGCACAGCCTGGGGGAGCTACTGCTGTTGCTG GCAGCGGAGCCCCCGGGCCGGAGGCGGGAGCTGCTTCACACTGAGGTTCAGAACCTCATGGCCCGAGCT GAATACTTGAAGGAGCAGGTCAAGATGAGGGAATCTCGCTGGGAAGCTGACACCCTGGACAAAGAGGGA CTGTCGGAATCTGTTCGTAGCTCTTGCACCCTTCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001099436 |
| Insert Size | 1419 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001099436.3 |
| RefSeq Size | 2644 bp |
| RefSeq ORF | 1419 bp |
| Locus ID | 25989 |
| UniProt ID | Q6PHR2 |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | mTOR signaling pathway, Regulation of autophagy |
| MW | 53.4 kDa |
| Gene Summary | Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (a). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220209 | ULK3 (Myc-DDK-tagged)-Human unc-51-like kinase 3 (C. elegans) (ULK3) |
CNY 3,656.00 |
|
| RC220209L1 | Lenti ORF clone of Human unc-51-like kinase 3 (C. elegans) (ULK3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC220209L2 | Lenti ORF clone of Human unc-51-like kinase 3 (C. elegans) (ULK3), mGFP tagged |
CNY 5,890.00 |
|
| RC220209L3 | Lenti ORF clone of Human unc-51-like kinase 3 (C. elegans) (ULK3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC220209L4 | Lenti ORF clone of Human unc-51-like kinase 3 (C. elegans) (ULK3), mGFP tagged |
CNY 5,890.00 |
|
| RG220209 | ULK3 (tGFP-tagged) - Human unc-51-like kinase 3 (C. elegans) (ULK3) |
CNY 4,370.00 |
|
| SC323667 | ULK3 (untagged)-Kinase deficient mutant (K87M) of Human unc-51-like kinase 3 (C. elegans) (ULK3) |
CNY 3,656.00 |
