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MIER1 (NM_001077701) Human Untagged Clone
CAT＃: SC315581
MIER1 (untagged)-Human mesoderm induction early response 1 homolog (Xenopus laevis) (MIER1), transcript variant 3
CNY 8,270.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ER1; MI-ER1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC315581 representing NM_001077701.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGAGCCATCTGTTGAATCTTCAAGTCCAGGAGGTTCAGCAACATCAGATGACCATGAATTTGAT CCATCAGCTGACATGCTGGTTCATGATTTTGATGATGAACGAACATTAGAAGAGGAAGAAATGATGGAA GGAGAAACAAACTTCAGCTCTGAAATAGAAGATCTTGCAAGGGAAGGCGACATGCCAATTCATGAACTT CTCAGCCTTTATGGTTATGGTAGTACTGTTCGACTACCTGAAGAAGATGAGGAAGAGGAAGAAGAGGAA GAAGAAGGTGAAGATGATGAAGATGCTGATAATGATGACAACAGTGGCTGTAGTGGGGAAAATAAAGAG GAGAATATAAAGGATTCATCAGGTCAGGAGGATGAAACTCAGTCTTCCAATGATGATCCATCACAATCT GTTGCTTCTCAAGATGCCCAGGAAATAATCCGCCCACGTCGATGTAAATATTTTGATACAAATAGTGAA GTAGAAGAAGAATCTGAAGAAGATGAAGATTATATTCCATCAGAAGACTGGAAAAAGGAGATTATGGTG GGCTCCATGTTTCAAGCAGAAATTCCAGTTGGCATTTGTAGATACAAAGAAAATGAAAAAGTATATGAA AATGATGATCAGCTCCTGTGGGACCCTGAGTACTTACCAGAAGATAAAGTGATTATATTTCTTAAAGAT GCATCTAGAAGAACAGGTGATGAGAAGGGTGTAGAAGCAATTCCTGAAGGATCTCACATAAAAGACAAT GAACAGGCTTTATATGAATTGGTTAAATGCAATTTTGATACAGAAGAAGCATTGAGAAGATTAAGATTT AATGTAAAAGCAGCTAGAGAGGAATTATCTGTTTGGACAGAGGAAGAGTGTAGAAATTTTGAACAAGGG CTGAAGGCCTATGGAAAGGATTTTCATTTGATTCAGGCTAATAAAGTCCGAACAAGGTCAGTTGGTGAA TGTGTAGCATTCTATTACATGTGGAAAAAATCTGAACGTTATGATTTCTTTGCTCAGCAAACACGATTT GGAAAGAAGAAATATAATCTTCATCCTGGTGTAACGGATTACATGGATCGTCTTCTAGACGAAAGTGAA AGTGCTGCATCTAGTCGAGCACCATCCCCTCCCCCAACTGCATCAAACAGTAGTAACAGCCAGTCTGAG AAAGAAGATGGCACTGTAAGCACTGCTAATCAAAATGGAGTGTCATCTAATGGACCAGGTGAAATATTA AACAAAGAGGAAGTAAAAGTTGAAGGGTTACACATTAATGGACCAACAGGTGGAAATAAGAAACCACTT CATGCAGATATGGATACTAATGGTTATGAAACAGATAACCTTACCACTGACCCAAAACTTGCCCATATG ACTGCAAGAAATGAAAATGATTTTGATGAAAAAAGTGAGAGACCTGCCAAAAGGCGAAGGGTAAACAGC AATGGAAAAGAAAGTCCAGGTTCTTCTGAATTTTTCCAAGAAGCAGTCTCACATGGGAAATTTGAAGAA CTTGAAAACACAGATGACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001077701 |
| Insert Size | 1539 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001077701.2 |
| RefSeq Size | 5425 bp |
| RefSeq ORF | 1539 bp |
| Locus ID | 57708 |
| UniProt ID | Q8N108 |
| MW | 58 kDa |
| Gene Summary | This gene encodes a protein that was first identified in Xenopus laevis by its role in a mesoderm induction early response (MIER). The encoded protein functions as a transcriptional regulator. Alternatively spliced transcript variants encode multiple isoforms, some of which lack a C-terminal nuclear localization signal. [provided by RefSeq, May 2013] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The resulting isoform (c, also known as N3-beta) has a shorter and distinct N-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223015 | MIER1 (Myc-DDK-tagged)-Human mesoderm induction early response 1 homolog (Xenopus laevis) (MIER1), transcript variant 3 |
CNY 3,816.00 |
|
| RC223015L1 | Lenti ORF clone of Human mesoderm induction early response 1 homolog (Xenopus laevis) (MIER1), transcript variant 3, Myc-DDK-tagged |
CNY 6,216.00 |
|
| RC223015L2 | Lenti ORF clone of Human mesoderm induction early response 1 homolog (Xenopus laevis) (MIER1), transcript variant 3, mGFP tagged |
CNY 6,460.00 |
|
| RC223015L3 | Lenti ORF clone of Human mesoderm induction early response 1 homolog (Xenopus laevis) (MIER1), transcript variant 3, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC223015L4 | Lenti ORF clone of Human mesoderm induction early response 1 homolog (Xenopus laevis) (MIER1), transcript variant 3, mGFP tagged |
CNY 6,460.00 |
|
| RG223015 | MIER1 (tGFP-tagged) - Human mesoderm induction early response 1 homolog (Xenopus laevis) (MIER1), transcript variant 3 |
CNY 5,040.00 |
