United States
Germany
Japan
United Kingdom
China
alpha 1,2 Mannosidase IA (MAN1A1) (NM_005907) Human Untagged Clone
CAT＃: SC314336
MAN1A1 (untagged)-Human mannosidase, alpha, class 1A, member 1 (MAN1A1)
CNY 10,450.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HUMM3; HUMM9; MAN9 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC314336 representing NM_005907.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCCGTGGGGGGCCTGTTGCCGCTCTTCAGCAGCCCCGCGGGCGGCGTCCTGGGCGGGGGGCTCGGC GGCGGCGGTGGCAGGAAGGGGTCGGGCCCCGCCGCCCTCCGCCTGACGGAGAAGTTCGTGCTGCTGCTG GTATTCAGCGCCTTCATCACGCTCTGCTTCGGGGCGATCTTCTTCCTGCCAGACTCCTCCAAGCTGCTC AGCGGGGTCCTGTTCCACTCCAGCCCCGCCTTGCAGCCGGCCGCCGACCACAAGCCCGGGCCCGGGGCG CGCGCCGAGGACGCGGCCGAGGGGCGAGCCCGGCGCCGCGAGGAGGGGGCACCCGGGGACCCGGAGGCC GCCCTGGAGGACAACTTGGCCAGGATCCGCGAAAACCACGAGCGGGCTCTCAGGGAAGCCAAGGAGACC CTGCAGAAGCTGCCCGAGGAGATCCAAAGAGACATCCTACTGGAGAAGAAGAAGGTGGCCCAGGACCAG CTGCGTGACAAGGCGCCGTTCAGAGGCCTGCCCCCGGTGGACTTCGTGCCCCCAATCGGGGTGGAGAGC CGGGAGCCCGCCGACGCCGCCATCCGCGAGAAAAGGGCAAAGATCAAAGAGATGATGAAACATGCTTGG AATAATTATAAAGGTTATGCCTGGGGATTAAATGAACTCAAACCTATATCAAAAGGAGGCCATTCAAGC AGTTTGTTTGGTAACATCAAAGGAGCAACTATAGTAGATGCCCTGGATACACTTTTTATTATGGAAATG AAACATGAATTTGAAGAAGCAAAATCATGGGTTGAAGAAAATTTAGATTTTAATGTGAATGCTGAAATT TCTGTCTTTGAAGTAAATATACGCTTTGTTGGTGGACTACTCTCAGCCTACTATCTGTCTGGAGAAGAG ATTTTTCGAAAGAAAGCAGTGGAACTTGGGGTAAAATTGCTACCTGCATTTCATACTCCCTCTGGAATA CCTTGGGCATTGCTGAATATGAAAAGTGGTATTGGAAGGAACTGGCCCTGGGCCTCTGGAGGCAGCAGT ATTCTGGCAGAATTTGGAACCCTGCATTTGGAGTTTATGCACTTGAGCCACTTATCAGGAAACCCCATC TTTGCTGAAAAGGTAATGAATATTCGAACAGTACTGAACAAACTGGAAAAACCACAAGGCCTTTATCCT AACTATCTGAATCCCAGTAGTGGACAGTGGGGTCAACATCATGTATCAGTTGGAGGACTTGGAGACAGC TTCTATGAGTATTTGCTGAAGGCCTGGTTAATGTCTGACAAGACAGATCTGGAAGCTAAGAAGATGTAT TTTGATGCTGTTCAGGCTATCGAGACTCATTTGATCCGCAAGTCTAGCAGCGGACTAACTTATATCGCA GAGTGGAAAGGGGGCCTCCTGGAGCACAAGATGGGCCACCTGACCTGCTTCGCGGGGGGCATGTTCGCA CTCGGGGCTGATGCAGCTCCCGAAGGCATGGCCCAACACTACCTTGAACTCGGGGCTGAAATTGCCCGT ACTTGTCATGAATCATATAATCGAACATTTATGAAACTGGGACCAGAAGCTTTCAGATTTGATGGTGGT GTTGAAGCCATCGCTACAAGACAAAATGAAAAATACTACATCTTACGGCCAGAAGTTATGGAGACTTAC ATGTATATGTGGAGACTGACTCATGATCCAAAGTACAGGAAATGGGCCTGGGAAGCCGTAGAGGCCTTG GAAAACCATTGCAGAGTGAATGGAGGCTATTCAGGCCTAAGGGATGTTTACCTTCTTCATGAGAGTTAT GATGATGTGCAGCAGAGTTTCTTCCTGGCAGAGACATTGAAATATTTGTACCTAATATTTTCTGACGAC GATCTTCTTCCACTGGAGCATTGGATCTTCAATAGCGAGGCACATCTTCTCCCTATCCTCCCTAAAGAT AAAAAGGAAGTTGAAATCAGAGAGGAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_005907 |
| Insert Size | 1962 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005907.3 |
| RefSeq Size | 5044 bp |
| RefSeq ORF | 1962 bp |
| Locus ID | 4121 |
| UniProt ID | P33908 |
| Domains | Glyco_hydro_47 |
| Protein Families | Transmembrane |
| Protein Pathways | Metabolic pathways, N-Glycan biosynthesis |
| MW | 73 kDa |
| Gene Summary | This gene encodes a class I mammalian Golgi 1,2-mannosidase which is a type II transmembrane protein. This protein catalyzes the hydrolysis of three terminal mannose residues from peptide-bound Man(9)-GlcNAc(2) oligosaccharides and belongs to family 47 of glycosyl hydrolases. [provided by RefSeq, Jul 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213002 | MAN1A1 (Myc-DDK-tagged)-Human mannosidase, alpha, class 1A, member 1 (MAN1A1) |
CNY 4,864.00 |
|
| RC213002L3 | Lenti-ORF clone of MAN1A1 (Myc-DDK-tagged)-Human mannosidase, alpha, class 1A, member 1 (MAN1A1) |
CNY 7,600.00 |
|
| RC213002L4 | Lenti-ORF clone of MAN1A1 (mGFP-tagged)-Human mannosidase, alpha, class 1A, member 1 (MAN1A1) |
CNY 7,600.00 |
|
| RG213002 | MAN1A1 (tGFP-tagged) - Human mannosidase, alpha, class 1A, member 1 (MAN1A1) |
CNY 6,270.00 |
其它alpha 1,2 Mannosidase IA产品
