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CHKL (CHKB) (NM_005198) Human Untagged Clone
CAT＃: SC313284
CHKB (untagged)-Human choline kinase beta (CHKB)
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CHETK; CHKL; CK; CKB; CKEKB; EK; EKB; MDCMC |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC313284 representing NM_005198.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGCCGAGGCGACAGCTGTGGCCGGAAGCGGGGCTGTTGGCGGCTGCCTGGCCAAAGACGGCTTG CAGCAGTCTAAGTGCCCGGACACTACCCCAAAACGGCGGCGCGCCTCGTCGCTGTCGCGTGACGCCGAG CGCCGAGCCTACCAATGGTGCCGGGAGTACTTGGGCGGGGCCTGGCGCCGAGTGCAGCCCGAGGAGCTG AGGGTTTACCCCGTGAGCGGAGGCCTCAGCAACCTGCTCTTCCGCTGCTCGCTCCCGGACCACCTGCCC AGCGTTGGCGAGGAGCCCCGGGAGGTGCTTCTGCGGCTGTACGGAGCCATCTTGCAGGGCGTGGACTCC CTGGTGCTAGAAAGCGTGATGTTCGCCATACTTGCGGAGCGGTCGCTGGGGCCCCAGCTGTACGGAGTC TTCCCAGAGGGCCGGCTGGAACAGTACATCCCAAGTCGGCCATTGAAAACTCAAGAGCTTCGAGAGCCA GTGTTGTCAGCAGCCATTGCCACGAAGATGGCGCAATTTCATGGCATGGAGATGCCTTTCACCAAGGAG CCCCACTGGCTGTTTGGGACCATGGAGCGGTACCTAAAACAGATCCAGGACCTGCCCCCAACTGGCCTC CCTGAGATGAACCTGCTGGAGATGTACAGCCTGAAGGATGAGATGGGCAACCTCAGGAAGTTACTAGAG TCTACCCCATCGCCAGTCGTCTTCTGCCACAATGACATCCAGGAAGGGAACATCTTGCTGCTCTCAGAG CCAGAAAATGCTGACAGCCTCATGCTGGTGGACTTCGAGTACAGCAGTTATAACTATAGGGGCTTTGAC ATTGGGAACCATTTTTGTGAGTGGGTTTATGATTATACTCACGAGGAATGGCCTTTCTACAAAGCAAGG CCCACAGACTACCCCACTCAAGAACAGCAGTTGCATTTTATTCGTCATTACCTGGCAGAGGCAAAGAAA GGTGAGACCCTCTCCCAAGAGGAGCAGAGAAAACTGGAAGAAGATTTGCTGGTAGAAGTCAGTCGGTAT GCTCTGGCATCCCATTTCTTCTGGGGTCTGTGGTCCATCCTCCAGGCATCCATGTCCACCATAGAATTT GGTTACTTGGACTATGCCCAGTCTCGGTTCCAGTTCTACTTCCAGCAGAAGGGGCAGCTGACCAGTGTC CACTCCTCATCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_005198 |
| Insert Size | 1188 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005198.4 |
| RefSeq Size | 1648 bp |
| RefSeq ORF | 1188 bp |
| Locus ID | 1120 |
| UniProt ID | Q9Y259 |
| Domains | Choline_kinase |
| Protein Families | Druggable Genome |
| Protein Pathways | Glycerophospholipid metabolism, Metabolic pathways |
| MW | 45.3 kDa |
| Gene Summary | Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/phosphoethanolamine. This is the first enzyme in the biosynthesis of phosphatidylcholine/phosphatidylethanolamine in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products have been shown to have EK activity also, indicating that both activities reside on the same protein. The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family; however, its exact function is not known. Read-through transcripts are expressed from this locus that include exons from the downstream CPT1B locus. [provided by RefSeq, Jun 2009] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210253 | CHKB (Myc-DDK-tagged)-Human choline kinase beta (CHKB) |
CNY 3,656.00 |
|
| RC210253L1 | Lenti ORF clone of Human choline kinase beta (CHKB), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210253L2 | Lenti ORF clone of Human choline kinase beta (CHKB), mGFP tagged |
CNY 5,890.00 |
|
| RC210253L3 | Lenti ORF clone of Human choline kinase beta (CHKB), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210253L4 | Lenti ORF clone of Human choline kinase beta (CHKB), mGFP tagged |
CNY 5,890.00 |
|
| RG210253 | CHKB (tGFP-tagged) - Human choline kinase beta (CHKB) |
CNY 4,370.00 |
