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TRPM5 (NM_014555) Human Untagged Clone
CAT＃: SC304125
TRPM5 (untagged)-Human transient receptor potential cation channel, subfamily M, member 5 (TRPM5)
CNY 8,120.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | LTRPC5; MTR1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC304125 sequence for NM_014555 edited (data generated by NextGen Sequencing)
ATGCAGGATGTCCAAGGCCCCCGTCCCGGAAGCCCCGGGGATGCTGAAGACCGGCGGGAGCTGGGCTTGC ACAGGGGCGAGGTCAACTTTGGAGGGTCTGGGAAGAAGCGAGGCAAGTTTGTACGGGTGCCGAGCGGAGT GGCCCCGTCTGTGCTCTTTGACCTGCTGCTTGCTGAGTGGCACCTGCCAGCCCCCAACCTGGTGGTGTCC CTGGTGGGTGAGGAGCAGCCTTTCGCCATGAAGTCCTGGCTGCGGGATGTGCTGCGCAAGGGGCTGGTGA AGGCGGCTCAGAGCACAGGAGCCTGGATCCTGACCAGTGCCCTCCGCGTGGGCCTGGCCAGGCATGTCGG GCAGGCCGTGCGCGACCACTCGCTGGCCAGCACGTCCACCAAGGTCCGTGTGGTTGCTGTCGGCATGGCC TCGCTGGGCCGCGTCCTGCACCGCCGCATTCTGGAGGAGGCCCAGGAGGATTTTCCTGTCCACTACCCTG AGGATGACGGCGGCAGCCAGGGCCCCCTCTGTTCACTGGACAGCAACCTCTCCCACTTCATCCTGGTGGA GCCAGGCCCCCCGGGGAAGGGCGATGGGCTGACGGAGCTGCGGCTGAGGCTGGAGAAGCACATCTCGGAG CAGAGGGCGGGCTACGGGGGCACTGGCAGCATCGAGATCCCTGTCCTCTGCTTGCTGGTCAATGGTGATC CCAACACCTTGGAGAGGATCTCCAGGGCCGTGGAGCAGGCTGCCCCGTGGCTGATCCTGGCAGGCTCGGG GGGCATCGCCGATGTGCTTGCTGCCCTAGTGAACCAGCCCCACCTCCTGGTGCCCAAGGTGGCCGAGAAG CAGTTTAAGGAGAAGTTCCCCAGCAAGCATTTCTCTTGGGAGGACATCGTGCGCTGGACCAAGCTGCTGC AGAACATCACCTCACACCAGCACCTGCTCACCGTGTATGACTTCGAGCAGGAGGGCTCCGAGGAGCTGGA CACGGTCATCCTGAAGGCGCTGGTGAAAGCCTGCAAGAGCCACAGCCAGGAGCCTCAGGACTATCTGGAT GAGCTCAAGCTGGCCGTGGCCTGGGACCGCGTGGACATCGCCAAGAGTGAGATCTTCAATGGGGACGTGG AGTGGAAGTCCTGTGACCTGGAGGAGGTGATGGTGGACGCCCTGGTCAGCAACAAGCCCGAGTTTGTGCG CCTCTTTGTGGACAACGGCGCAGACGTGGCCGACTTCCTGACGTATGGGCGGCTGCAGGAGCTCTACCGC TCTGTGTCACGCAAGAGCCTGCTCTTCGACCTGCTGCAGCGGAAGCAGGAGGAGGCCCGGCTGACGCTGG CCGGCCTGGGCACCCAGCAGGCCCGGGAGCCACCCGCGGGGCCACCGGCCTTCTCCCTGCACGAGGTCTC CCGCGTACTCAAGGACTTCCTGCAGGACGCCTGCCGAGGCTTCTACCAGGACGGCCGGCCAGGGGACCGC AGGAGGGCGGAGAAGGGCCCGGCCAAGCGGCCCACGGGCCAGAAGTGGCTGCTGGACCTGAACCAGAAGA GCGAGAACCCCTGGCGGGACCTGTTCCTGTGGGCCGTGCTGCAGAACCGCCACGAGATGGCCACCTACTT CTGGGCCATGGGCCAGGAAGGTGTGGCAGCCGCACTGGCCGCCTGCAAAATCCTCAAAGAGATGTCGCAC CTGGAGACGGAGGCCGAGGCGGCCCGAGCCACGCGCGAGGCGAAATACGAGCGGCTGGCCCTTGACCTCT TCTCCGAGTGCTACAGCAACAGTGAGGCCCGCGCCTTCGCCCTGCTGGTGCGCCGGAACCGCTGCTGGAG CAAGACCACCTGCCTGCACCTGGCCACCGAGGCTGACGCCAAGGCCTTCTTTGCCCACGACGGCGTTCAG GCCTTCCTGACCAGGATCTGGTGGGGGGACATGGCCGCAGGCACGCCCATCCTGCGGCTGCTAGGAGCCT TCCTCTGCCCCGCCCTCGTCTATACCAACCTCATCACCTTCAGTGAGGAAGCTCCCCTGAGGACAGGCCT GGAGGACCTGCAGGACCTGGACAGCCTGGACACGGAGAAGAGCCCGCTGTATGGCCTGCAGAGCCGGGTG GAGGAGCTGGTGGAGGCGCCGAGGGCTCAGGGTGACCGAGGCCCACGTGCTGTCTTCCTGCTCACACGCT GGCGGAAATTCTGGGGCGCTCCCGTGACTGTGTTCCTGGGGAACGTGGTCATGTACTTCGCCTTCCTCTT CCTGTTCACCTACGTCCTGCTGGTGGACTTCAGGCCGCCCCCCCAGGGCCCCTCAGGGCCCGAGGTCACC CTCTACTTCTGGGTCTTTACGCTGGTGCTGGAGGAAATCCGGCAGGGCTTCTTCACAGACGAGGACACAC ACCTGGTGAAGAAGTTCACACTGTATGTGGGGGACAACTGGAACAAGTGTGACATGGTGGCCATCTTCCT GTTCATCGTGGGTGTCACCTGCAGGATGCTGCCGTCGGCGTTTGAGGCTGGCCGCACAGTCCTCGCCATG GACTTCATGGTGTTCACGCTGCGGCTGATCCATATCTTTGCCATACACAAGCAGCTGGGCCCCAAGATCA TCGTGGTAGAGCGCATGATGAAGGACGTCTTCTTCTTCCTCTTCTTTCTGAGCGTGTGGCTCGTGGCCTA CGGTGTCACCACCCAGGCGCTGCTGCACCCCCATGACGGCCGCCTGGAGTGGATCTTCCGCCGGGTGCTC TACCGGCCCTACCTGCAGATCTTCGGCCAGATCCCACTGGACGAGATTGATGAAGCCCGTGTGAACTGCT CCACCCACCCACTGCTGCTGGAGGACTCACCATCCTGCCCCAGCCTCTATGCCAACTGGCTGGTCATCCT CCTGCTGGTCACCTTCCTGTTGGTCACCAATGTGCTGCTCATGAACCTGCTCATCGCCATGTTCAGCTAC ACGTTCCAGGTGGTGCAGGGCAACGCAGACATGTTCTGGAAGTTCCAGCGCTACAACCTGATTGTGGAGT ACCACGAGCGCCCCGCCCTGGCCCCGCCCTTCATCCTGCTCAGCCACCTGAGCCTGACGCTCCGCCGGGT CTTCAAGAAGGAGGCTGAGCACAAGCGGGAGCACCTGGAGAGAGACCTGCCAGACCCCCTGGACCAGAAG GTCGTCACCTGGGAGACAGTCCAGAAGGAGAACTTCCTGAGCAAGATGGAGAAGCGGAGGAGGGACAGCG AGGGGGAGGTGCTGCGGAAAACCGCCCACAGAGTGGACTTCATTGCCAAGTACCTCGGGGGTCTGAGAGA GCAAGAAAAGCGCATCAAGTGTCTGGAGTCACAGATCAACTACTGCTCGGTGCTCGTGTCCTCCGTGGCT GACGTGCTGGCCCAGGGTGGCGGCCCCCGGAGCTCTCAGCACTGTGGCGAGGGAAGCCAGCTGGTGGCTG CTGACCACAGAGGTGGTTTAGATGGCTGGGAACAACCCGGGGCTGGCCAGCCTCCCTCGGACACCTGA Clone variation with respect to NM_014555.3 189:a=>g 761:c=>t 1263:t=>c |
| Restriction Sites | Please inquire |
| ACCN | NM_014555 |
| Insert Size | 3500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The ORF of this clone has been fully sequenced and found to be a good match to NM_014555.2 except one SNP. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014555.2, NP_055370.1 |
| RefSeq Size | 3946 bp |
| RefSeq ORF | 3498 bp |
| Locus ID | 29850 |
| UniProt ID | Q9NZQ8 |
| Protein Families | Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane |
| Protein Pathways | Taste transduction |
| Gene Summary | This gene encodes a member of the transient receptor potential (TRP) protein family, which is a diverse group of proteins with structural features typical of ion channels. This protein plays an important role in taste transduction, and has characteristics of a calcium-activated, non-selective cation channel that carries Na+, K+, and Cs+ ions equally well, but not Ca(2+) ions. It is activated by lower concentrations of intracellular Ca(2+), and inhibited by higher concentrations. It is also a highly temperature-sensitive, heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. This gene is located within the Beckwith-Wiedemann syndrome critical region-1 on chromosome 11p15.5, and has been shown to be imprinted, with exclusive expression from the paternal allele. [provided by RefSeq, Oct 2010] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Sensory Attributes of Complex Tasting Divalent Salts Are Mediated by TRPM5 and TRPV1 Channels
,Céline E. Riera, Horst Vogel, Sidney A. Simon, Sami Damak, and Johannes le Coutre,
J. Neurosci., Feb 2009; 29: 2654 - 2662.
[TRPM5]
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| RC210840L2 | Lenti-ORF clone of TRPM5 (mGFP-tagged)-Human transient receptor potential cation channel, subfamily M, member 5 (TRPM5) |
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| RC210840L3 | Lenti-ORF clone of TRPM5 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily M, member 5 (TRPM5) |
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| RC210840L4 | Lenti-ORF clone of TRPM5 (mGFP-tagged)-Human transient receptor potential cation channel, subfamily M, member 5 (TRPM5) |
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| RG210840 | TRPM5 (tGFP-tagged) - Human transient receptor potential cation channel, subfamily M, member 5 (TRPM5) |
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