Myosin 8 (MYH8) (NM_002472) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DA7; gtMHC-F; MyHC-peri; MyHC-pn
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC303205 representing NM_002472. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGTGCGAGCTCAGACGCTGAGATGGCTGTTTTTGGCGAAGCTGCTCCCTACCTTCGAAAATCAGAA AAGGAGCGGATTGAGGCCCAAAACAAGCCGTTTGATGCTAAAACATCTGTCTTTGTGGCGGAGCCCAAG GAATCCTATGTGAAGAGCACTATACAAAGCAAAGAAGGAGGGAAAGTAACCGTAAAGACTGAAGGTGGA GCAACTCTAACTGTCAGGGAAGACCAAGTCTTCCCTATGAACCCTCCGAAATATGACAAAATTGAGGAC ATGGCCATGATGACTCATCTACACGAGCCTGGAGTGCTGTACAACCTCAAAGAGCGCTATGCAGCCTGG ATGATCTACACCTACTCAGGCCTCTTCTGTGTCACCGTCAACCCCTACAAGTGGCTGCCGGTGTACAAG CCCGAGGTGGTGGCTGCCTACAGAGGCAAAAAGCGCCAGGAGGCCCCGCCCCACATCTTCTCCATCTCT GACAATGCCTATCAGTTCATGTTGACTGATCGAGAGAATCAGTCCATCCTGATCACCGGAGAATCTGGT GCCGGAAAGACTGTGAACACCAAGCGTGTCATCCAATACTTTGCAACAATTGCAGTTACTGGAGAGAAG AAGAAGGATGAATCTGGCAAAATGCAGGGGACTCTGGAAGATCAAATCATCAGCGCCAATCCCCTACTG GAGGCCTTTGGCAATGCCAAAACTGTGAGGAATGACAACTCCTCTCGCTTTGGTAAATTCATTAGAATC CACTTTGGTACTACAGGGAAGCTGGCATCTGCTGATATAGAAACATATCTTTTAGAAAAGTCCAGAGTT ACTTTCCAGCTAAAGGCGGAAAGAAGCTACCATATTTTTTATCAGATCACTTCCAATAAGAAGCCAGAT CTAATTGAAATGCTCCTGATCACCACCAACCCATATGACTATGCCTTCGTCAGTCAGGGGGAGATCACA GTTCCCAGTATTGATGACCAAGAAGAGTTGATGGCCACTGATAGTGCCATTGACATCCTGGGCTTCACT CCTGAAGAGAAAGTGTCCATCTATAAACTCACAGGGGCTGTGATGCATTATGGGAACATGAAATTCAAG CAAAAGCAGCGTGAGGAGCAAGCTGAGCCAGATGGCACAGAAGTCGCTGACAAGGCAGCCTATCTCCAG AGTCTGAACTCTGCAGACCTACTCAAAGCCCTCTGCTACCCTAGGGTCAAGGTTGGCAATGAGTATGTC ACCAAAGGCCAGACTGTGCAGCAGGTGTACAATGCGGTGGGTGCTCTGGCCAAAGCCGTCTACGAGAAG ATGTTCCTGTGGATGGTCACCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCAGGCAGTACTTCATC GGGGTCTTGGACATTGCTGGCTTTGAAATCTTTGATTTTAACAGCCTGGAGCAGCTGTGCATCAACTTC ACCAACGAGAAACTGCAACAGTTTTTCAACCACCACATGTTTGTGCTAGAGCAGGAGGAGTACAAGAAG GAAGGCATCGAGTGGACGTTCATTGACTTTGGGATGGACCTGGCTGCCTGCATTGAGCTCATTGAGAAG CCACTGGGCATCTTCTCCATCCTGGAAGAGGAGTGCATGTTCCCTAAGGCAACGGACACCTCCTTCAAG AACAAGCTGTATGACCAGCACCTGGGCAAGTCTGCCAACTTCCAGAAGCCCAAGGTGGTCAAAGGCAAG GCTGAGGCCCACTTCTCTCTGATTCACTATGCTGGCACTGTGGACTACAACATTACTGGCTGGCTGGAC AAAAATAAGGACCCCCTGAATGATACTGTGGTTGGGCTGTACCAGAAGTCTGCAATGAAGACTCTAGCC AGTCTCTTTTCCACGTATGCTAGTGCTGAAGCAGATAGCAGCGCGAAGAAAGGTGCTAAGAAAAAGGGC TCTTCTTTCCAGACTGTGTCTGCCCTTTTCAGGGAAAATTTAAATAAATTGATGACGAATCTGAGGAGC ACACACCCTCACTTCGTACGGTGTATCATTCCCAATGAAACCAAAACTCCTGGGGCAATGGAACATGAA CTTGTGTTGCACCAGCTGAGGTGTAATGGTGTGCTGGAAGGCATCCGCATCTGTAGGAAAGGATTCCCA AGCAGAATCTTATATGGTGATTTCAAACAAAGATACAAGGTTTTAAATGCAAGTGCTATTCCAGAGGGA CAGTTCATTGACAGCAAGAAGGCTTCTGAGAAACTTCTTGCATCTATTGATATTGATCATACTCAATAT AAATTTGGACATACCAAGGTTTTCTTCAAAGCTGGACTTCTGGGTCTTCTGGAAGAAATGAGAGATGAA AAATTAGCCCAAATTATAACAAGAACACAAGCTGTCTGTAGGGGATTCCTAATGAGGGTAGAATATCAG AAGATGTTGCAAAGGAGAGAAGCACTTTTCTGCATCCAGTATAATGTCCGTGCCTTCATGAACGTCAAG CACTGGCCCTGGATGAAACTCTTTTTCAAGATTAAGCCCCTCCTCAAGAGTGCAGAGACCGAGAAAGAG ATGGCCACCATGAAGGAAGAATTCCAGAAAACCAAAGATGAACTCGCCAAGTCAGAGGCAAAACGGAAG GAGCTAGAGGAAAAAATGGTCACTCTCTTAAAAGAGAAAAATGACCTGCAACTCCAGGTTCAATCTGAA GCAGATAGCTTGGCTGATGCAGAGGAAAGGTGTGAGCAACTGATTAAAAACAAAATCCAACTTGAGGCC AAAATCAAAGAGGTGACTGAAAGAGCTGAGGAGGAGGAAGAGATCAATGCTGAGCTGACAGCCAAGAAG AGAAAACTGGAGGATGAATGTTCAGAACTCAAGAAAGACATTGATGACCTTGAGCTGACACTGGCCAAG GTTGAGAAGGAGAAACATGCCACGGAGAACAAGGTGAAAAATCTTACAGAAGAGATGGCAGGCCTGGAT GAAACCATTGCAAAACTGTCCAAGGAGAAGAAGGCTCTCCAAGAGACCCACCAGCAGACCCTGGATGAC CTGCAGGCAGAGGAGGACAAAGTCAACATCCTGACCAAAGCTAAAACCAAGCTAGAACAGCAAGTGGAT GATCTTGAAGGGTCTCTGGAACAAGAAAAGAAGCTTCGAATGGATCTAGAAAGAGCAAAGCGGAAACTG GAGGGTGACCTCAAATTGGCCCAAGAATCCACAATGGATATGGAAAATGACAAACAGCAACTTGATGAA AAGCTTGAAAAGAAAGAATTTGAAATCAGCAATTTGATAAGCAAAATTGAAGATGAGCAAGCTGTAGAA ATTCAACTACAGAAGAAGATCAAAGAGTTGCAGGCCCGCATTGAGGAGCTGGGGGAAGAAATCGAGGCA GAGAGGGCGTCCCGAGCCAAAGCGGAGAAGCAGCGCTCTGACCTCTCCCGGGAACTGGAGGAGATCAGC GAGAGGCTGGAAGAAGCCGGTGGGGCAACTTCTGCTCAGGTGGAATTGAACAAGAAGCGGGAGGCTGAG TTTCAGAAACTGCGCAGGGACCTGGAGGAGGCCACCCTGCAGCATGAAGCTATGGTGGCTGCTCTTCGG AAGAAGCACGCAGACAGTATGGCTGAGCTTGGGGAGCAGATTGACAACTTGCAGCGGGTCAAACAGAAG CTGGAGAAGGAGAAGAGTGAGCTGAAGATGGAGACTGATGACCTCAGCAGTAACGCAGAGGCCATTTCC AAAGCCAAGGGAAACCTTGAAAAGATGTGCCGCTCTCTAGAAGATCAAGTGAGTGAGCTTAAGACCAAG GAAGAGGAGCAGCAGCGGCTGATCAATGACCTCACAGCACAGAGAGCGCGCCTGCAGACAGAAGCGGGT GAATATTCTCGACAATTAGATGAGAAAGATGCTTTAGTCTCTCAGCTTTCAAGGAGCAAGCAAGCATCT ACTCAGCAGATTGAAGAGCTGAAACATCAACTAGAGGAAGAAACTAAAGCCAAGAACGCCCTGGCACAC GCCCTGCAGTCCTCCCGCCATGACTGCGACCTGCTGCGGGAACAGTATGAGGAAGAGCAGGAAGGCAAA GCTGAGCTGCAGAGGGCGCTGTCCAAGGCCAACAGTGAGGTTGCCCAGTGGAGAACCAAATACGAGACG GATGCCATCCAGCGCACAGAGGAGCTGGAGGAGGCCAAGAAAAAGTTGGCCCAGCGCCTGCAAGAAGCT GAGGAACATGTAGAAGCTGTGAACGCCAAATGTGCTTCCCTTGAGAAGACGAAGCAGCGGCTCCAGAAT GAAGTTGAAGACCTCATGCTTGATGTGGAAAGGTCTAATGCAGCCTGTGCAGCCCTTGATAAGAAGCAA AGGAACTTTGACAAGGTCCTATCAGAATGGAAGCAGAAGTATGAGGAAACTCAGGCTGAACTTGAGGCC TCCCAGAAGGAGTCACGTTCTCTTAGCACTGAGCTGTTCAAGGTGAAGAATGTCTATGAGGAATCCCTG GATCAACTCGAAACGCTAAGAAGAGAAAATAAGAACTTGCAACAGGAGATTTCTGACCTCACTGAGCAG ATTGCAGAGGGAGGAAAGCAAATTCATGAATTGGAGAAAATAAAGAAGCAAGTAGAACAAGAGAAATGT GAAATTCAGGCTGCTTTAGAGGAAGCAGAGGCATCTCTTGAACATGAAGAAGGAAAGATTCTGCGTATC CAGCTTGAGTTAAACCAAGTCAAGTCTGAAGTTGATAGAAAAATCGCAGAAAAGGATGAGGAAATTGAC CAGCTGAAGAGAAACCACACTAGAGTCGTGGAGACAATGCAGAGCACGCTGGATGCAGAGATTAGAAGC AGAAATGATGCTCTGAGAGTCAAGAAGAAAATGGAAGGAGATCTGAATGAAATGGAAATCCAGCTGAAC CATGCCAATCGCTTAGCTGCAGAGAGTTTAAGGAACTACAGGAACACCCAAGGAATCCTGAAGGAAACC CAGCTCCACCTGGATGATGCTCTCCGGGGCCAGGAGGACCTCAAGGAACAGCTGGCAATTGTGGAGCGC AGAGCCAACCTGCTGCAGGCTGAGATCGAGGAGCTGTGGGCCACTCTGGAACAGACAGAGAGAAGCAGG AAAATCGCCGAACAGGAGCTCCTGGATGCCAGTGAGCGTGTCCAGCTCCTCCACACCCAGAATACCAGT CTCATTAACACCAAGAAGAAATTAGAAAATGACGTTTCCCAACTCCAAAGTGAAGTGGAAGAAGTAATC CAAGAATCACGCAATGCAGAAGAGAAAGCCAAGAAGGCCATCACTGATGCTGCCATGATGGCTGAGGAG CTGAAGAAGGAACAGGACACCAGCGCCCACCTGGAGCGGATGAAGAAGAACCTGGAGCAGACGGTGAAG GACCTGCAGCATCGTCTAGATGAGGCCGAGCAGCTGGCGCTGAAGGGTGGGAAGAAGCAGATCCAGAAA CTGGAGGCCAGGGTACGTGAGCTTGAAGGAGAGGTTGAAAATGAACAGAAACGTAATGCAGAGGCTGTT AAAGGTTTACGGAAACATGAGCGACGAGTAAAAGAACTCACCTACCAGACTGAAGAAGATCGCAAGAAT GTTCTCAGGCTGCAGGACTTGGTAGATAAATTACAGGCGAAGGTGAAATCATACAAGAGACAAGCTGAG GAGGCTGAGGAACAATCCAATGCTAATCTATCTAAATTCCGCAAACTCCAGCATGAGCTGGAGGAGGCC GAGGAACGGGCTGACATTGCTGAGTCCCAGGTCAACAAATTGCGAGTGAAGAGCCGAGAGGTTCACACA AAAATCAGTGCAGAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_002472
Insert Size	5814 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_002472.2
RefSeq Size	6038 bp
RefSeq ORF	5814 bp
Locus ID	4626
UniProt ID	P13535
Protein Pathways	Tight junction, Viral myocarditis
MW	222.8 kDa
Gene Summary	Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells. Muscle myosins are heterohexamers composed of 2 myosin heavy chains and 2 pairs of nonidentical myosin light chains. This gene encodes a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction. This gene is predominantly expressed in fetal skeletal muscle. This gene is found in a cluster of myosin heavy chain genes on chromosome 17. A mutation in this gene results in trismus-pseudocamptodactyly syndrome. [provided by RefSeq, Sep 2009]

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