Collagen IV alpha 6 (COL4A6) (NM_001847) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CXDELq22.3; DELXq22.3; DFNX6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC303081 representing NM_001847. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTTATAAACAAGTTGTGGCTGCTCCTGGTTACGTTGTGCCTGACCGAGGAACTGGCAGCAGCGGGA GAGAAGTCTTATGGAAAGCCATGTGGGGGCCAGGACTGCAGTGGGAGCTGTCAGTGTTTTCCTGAGAAA GGAGCGAGAGGACGACCTGGACCAATTGGAATTCAAGGCCCAACAGGTCCTCAAGGATTCACTGGCTCT ACTGGTTTATCGGGATTGAAAGGAGAAAGGGGTTTCCCAGGCCTTCTGGGACCTTATGGACCAAAAGGA GATAAGGGTCCCATGGGAGTTCCTGGCTTTCTTGGCATCAATGGGATTCCGGGCCACCCTGGACAACCA GGCCCCAGAGGCCCACCTGGTCTGGATGGCTGTAATGGAACTCAAGGAGCTGTTGGATTTCCAGGCCCT GATGGCTATCCTGGGCTTCTCGGACCACCCGGGCTTCCTGGTCAGAAAGGATCAAAAGGTGACCCTGTC CTTGCTCCAGGTAGTTTCAAAGGAATGAAGGGGGATCCTGGGCTGCCTGGACTGGATGGAATCACTGGC CCACAAGGAGCACCCGGATTTCCTGGAGCTGTAGGACCTGCAGGACCACCAGGATTACAAGGTCCTCCA GGGCCTCCTGGTCCTCTTGGTCCTGATGGGAATATGGGGCTAGGTTTTCAAGGAGAGAAAGGAGTCAAG GGGGATGTTGGCCTCCCTGGCCCAGCAGGACCTCCACCATCTACTGGAGAGCTGGAATTCATGGGATTC CCCAAAGGGAAGAAAGGATCCAAGGGTGAACCAGGGCCTAAGGGTTTTCCAGGCATAAGTGGCCCTCCA GGCTTCCCGGGCCTTGGAACTACTGGAGAAAAGGGAGAAAAGGGAGAAAAGGGAATCCCTGGTTTGCCA GGACCTAGGGGTCCCATGGGTTCAGAAGGAGTCCAAGGCCCTCCAGGGCAACAGGGCAAGAAAGGGACC CTGGGATTTCCTGGGCTTAATGGATTCCAAGGAATTGAGGGTCAAAAGGGTGACATTGGCCTGCCAGGC CCAGATGTTTTCATCGATATAGATGGTGCTGTGATCTCAGGTAATCCTGGAGATCCTGGTGTACCTGGC CTCCCAGGCCTTAAAGGAGATGAAGGCATCCAAGGCCTACGTGGCCCTTCTGGTGTCCCTGGATTGCCA GCATTATCAGGTGTCCCAGGAGCCCTAGGGCCTCAGGGATTTCCAGGGCTGAAGGGGGACCAAGGAAAC CCAGGCCGTACCACAATTGGAGCAGCTGGCCTCCCTGGCAGAGATGGTTTGCCAGGCCCACCAGGTCCA CCAGGCCCACCTAGTCCAGAATTTGAGACTGAAACTCTACACAACAAAGAGTCAGGGTTCCCTGGTCTC CGAGGAGAACAAGGTCCAAAAGGAAACCTAGGCCTCAAAGGAATAAAAGGAGACTCAGGTTTCTGTGCT TGTGACGGTGGTGTTCCCAACACTGGACCACCCGGGGAACCAGGCCCACCTGGTCCATGGGGTCTCATA GGCCTTCCAGGCCTTAAAGGAGCCAGAGGAGATCGAGGCTCTGGGGGTGCACAGGGCCCAGCAGGGGCT CCAGGCTTAGTTGGGCCTCTGGGTCCTTCAGGACCCAAAGGAAAGAAGGGGGAACCAATTCTCAGTACA ATCCAAGGAATGCCAGGAGATCGGGGTGATTCTGGCTCCCAGGGCTTCCGTGGTGTAATAGGAGAACCA GGCAAGGACGGAGTACCAGGTTTACCAGGTCTGCCAGGCCTTCCGGGTGATGGTGGACAGGGCTTCCCA GGTGAAAAGGGGTTACCTGGACTTCCTGGTGAAAAAGGCCATCCTGGTCCACCTGGCCTCCCAGGAAAT GGGTTACCAGGACTTCCTGGACCCCGTGGGCTTCCTGGAGATAAAGGCAAGGATGGATTACCGGGACAA CAAGGCCTTCCCGGATCTAAGGGAATCACCCTGCCCTGTATTATTCCTGGGTCATACGGTCCATCAGGA TTTCCAGGCACTCCCGGATTCCCAGGCCCTAAAGGGTCTCGAGGCCTCCCTGGGACCCCAGGCCAGCCT GGGTCAAGTGGAAGTAAAGGAGAGCCAGGGAGTCCAGGATTGGTTCATCTTCCTGAATTACCAGGATTT CCTGGACCTCGTGGGGAGAAGGGCTTGCCTGGGTTTCCTGGGCTCCCTGGAAAAGATGGCTTGCCTGGG ATGATTGGCAGTCCAGGCTTACCTGGTTCCAAGGGAGCCACTGGTGACATCTTTGGTGCTGAAAATGGT GCTCCGGGGGAACAAGGCCTACAAGGATTAACAGGGCACAAAGGATTTCTTGGAGACTCTGGCCTTCCA GGACTCAAGGGTGTGCACGGGAAGCCTGGCTTACTAGGCCCCAAAGGTGAGCGGGGCAGCCCTGGGACA CCAGGACAGGTGGGACAGCCAGGCACCCCAGGATCTAGTGGTCCATATGGCATCAAGGGCAAATCTGGG CTCCCAGGAGCACCAGGCTTCCCAGGCATCTCAGGACATCCTGGAAAGAAAGGAACAAGAGGCAAGAAA GGTCCTCCTGGATCAATTGTAAAGAAAGGGCTGCCAGGGCTAAAAGGCCTTCCTGGAAATCCAGGCCTA GTAGGACTGAAAGGAAGCCCAGGCTCTCCAGGGGTCGCTGGGTTGCCAGCCCTCTCTGGACCCAAGGGA GAGAAGGGGTCTGTTGGATTCGTAGGTTTTCCAGGAATACCAGGTCTGCCTGGTATTCCTGGAACAAGA GGATTAAAAGGAATTCCAGGATCAACTGGAAAAATGGGACCATCTGGACGTGCTGGTACTCCTGGTGAA AAGGGAGACAGAGGCAATCCGGGGCCAGTCGGAATACCTAGTCCAAGACGTCCAATGTCAAACCTTTGG CTCAAAGGAGACAAAGGCTCTCAAGGCTCAGCCGGATCCAATGGATTTCCTGGGCCAAGAGGTGACAAA GGAGAGGCTGGTCGACCTGGACCACCAGGCCTACCTGGAGCTCCTGGCCTCCCAGGCATTATCAAAGGA GTTAGTGGAAAGCCAGGGCCCCCTGGCTTCATGGGAATCCGGGGCTTACCTGGCCTGAAGGGGTCCTCT GGGATCACAGGTTTCCCAGGAATGCCAGGAGAAAGTGGTTCACAAGGTATCAGAGGGTCGCCTGGACTC CCAGGAGCATCTGGTCTCCCAGGCCTGAAAGGAGACAACGGCCAGACAGTTGAAATTTCCGGTAGCCCA GGACCCAAGGGACAGCCTGGCGAATCTGGTTTTAAAGGCACAAAAGGAAGAGATGGACTAATAGGCAAT ATAGGCTTCCCTGGAAACAAAGGTGAAGATGGAAAAGTTGGTGTTTCTGGAGATGTTGGCCTTCCTGGA GCTCCAGGATTTCCAGGAGTTGCCGGCATGAGAGGAGAACCAGGACTTCCAGGTTCTTCTGGTCACCAA GGGGCAATTGGGCCTCTAGGATCCCCCGGATTAATAGGACCCAAAGGCTTCCCTGGATTTCCTGGTTTA CATGGACTGAATGGGCTTCCGGGCACCAAGGGTACCCATGGCACTCCAGGACCTAGTATCACCGGTGTG CCTGGGCCTGCTGGTCTCCCTGGACCCAAAGGAGAAAAAGGATATCCAGGAATTGGCATCGGAGCTCCA GGGAAGCCGGGCCTGAGAGGGCAAAAAGGTGATCGAGGTTTCCCAGGTCTCCAGGGCCCTGCTGGTCTC CCCGGTGCCCCAGGCATCTCCTTGCCCTCACTCATAGCAGGACAGCCTGGTGACCCCGGGCGACCAGGC CTAGATGGAGAACGAGGCCGCCCAGGCCCCGCTGGACCCCCAGGTCCCCCTGGGCCATCCTCGAATCAA GGCGACACCGGAGACCCTGGCTTCCCTGGAATTCCTGGACCTAAAGGGCCTAAGGGAGACCAAGGAATT CCAGGTTTTTCTGGCCTCCCTGGAGAGCTAGGACTGAAAGGCATGAGAGGTGAGCCTGGCTTCATGGGG ACTCCAGGCAAGGTTGGGCCACCTGGAGACCCAGGATTTCCCGGAATGAAGGGGAAGGCAGGGCCAAGA GGCTCTTCTGGCCTCCAAGGTGATCCTGGACAAACACCAACTGCAGAAGCTGTCCAGGTTCCTCCTGGA CCCTTGGGTCTACCAGGGATCGATGGCATCCCTGGCCTCACTGGGGACCCTGGGGCTCAAGGCCCTGTA GGCCTACAAGGCTCCAAAGGTTTACCTGGCATCCCCGGTAAAGATGGCCCCAGTGGGCTCCCAGGCCCA CCTGGGGCTCTTGGTGATCCTGGTCTGCCTGGACTGCAAGGCCCTCCAGGATTTGAAGGAGCTCCAGGG CAGCAAGGCCCCTTCGGGATGCCTGGAATGCCTGGCCAGAGCATGAGAGTGGGCTACACGTTGGTAAAG CACAGCCAGTCGGAACAGGTGCCCCCGTGTCCCATCGGGATGAGCCAGCTGTGGGTGGGGTACAGCTTA CTGTTTGTGGAGGGGCAAGAGAAAGCCCACAACCAGGACCTGGGCTTTGCTGGCTCCTGTCTGCCCCGC TTCAGCACCATGCCCTTCATCTACTGCAACATCAACGAGGTGTGCCACTATGCCAGGCGCAATGATAAA TCTTACTGGCTCTCCACTACCGCCCCTATCCCCATGATGCCCGTCAGCCAGACCCAGATTCCCCAGTAC ATCAGCCGCTGCTCTGTGTGTGAGGCACCCTCGCAAGCCATTGCTGTGCACAGCCAGGACATCACCATC CCGCAGTGCCCCCTGGGCTGGCGCAGCCTCTGGATTGGGTACTCTTTCCTCATGCACACTGCCGCTGGT GCCGAGGGTGGAGGCCAGTCCCTGGTCTCACCTGGCTCCTGCCTAGAGGACTTTCGGGCCACTCCTTTC ATCGAATGCAGTGGTGCCCGAGGCACCTGCCACTACTTTGCAAACAAGTACAGTTTCTGGTTGACCACA GTGGAGGAGAGGCAGCAGTTTGGGGAGTTGCCTGTGTCTGAAACGCTGAAAGCTGGGCAGCTCCACACT CGAGTCAGTCGCTGCCAGGTGTGTATGAAAAGCCTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001847
Insert Size	5076 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001847.3
RefSeq Size	6618 bp
RefSeq ORF	5076 bp
Locus ID	1288
UniProt ID	Q14031
Protein Pathways	ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer
MW	163.8 kDa
Gene Summary	This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene, alpha 5 type IV collagen, so that the gene pair shares a common promoter. Deletions in the alpha 5 gene that extend into the alpha 6 gene result in diffuse leiomyomatosis accompanying the X-linked Alport syndrome caused by the deletion in the alpha 5 gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013] Transcript Variant: This variant (A) utilizes a different alternative exon in the 5' UTR and coding region, exon 1A, which results in use of an alternate upstream translation start, compared to variant 3. The resulting protein (isoform A) has a distinct and shorter N-terminus, compared to isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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