EAAT2 (SLC1A2) (AK090810) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EAAT2; GLT-1; HBGT
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK090810, the custom clone sequence may differ by one or more nucleotides AAAGTGCTGGGATTACAGGCGTGAGCCACCGTGCCCGGCCAGAAAATACATTCTTTACAGCAAATGTTAT AGGTTAAGGAGACAGTTCCTGGGTTTAGCTGTGAAACTGTTTAGGATTTTCTTTAAGTGGTGTTCCCAAG AGCTTTTTTCTGGTAAGTTGGAGGCTATAGGCAAAGCGTCTAGGAGCTTGTTGTTTTTTGCTATGGAAGG CCAGCTTTGGGATCTATATACATGGGCATGTTTCTCAATGGAGTGGTCACAGGATTCTCTTCTCAGAACT CTGCCTGAAACCTTTCCGGTGTGTTTTGTCTTCAACTTTTTCCTAGCTATCCTATTAGCCTGTAAATATG TCAGCAGACAATGTGTTTGGAAGAAACAAGGTGTACAACTTCACAAAGCAAGTTTTTTCTGGTCTGAGAC TTACTGTGTGCAGGAAGTCTCGTGAGAATATACTTAGAAAATCACACTTATCTGGAAATTCAATAAGTCA TATTAAAAATATACAATTTCTTGAACTCAGGACTAGCCTGAAAATCCAAAATGCATGTAGAGTTGACATG TTGACATATGTCTATGTCCTCTGTTTTTATGTAAGCCTCATTTATTTCTCCCTTGCCATGCCCATTTGGG ATTTTCTTTGCCCAGCATATGTGCCATAAACAGAACAAATGGATCCAGGGTATACCTCTAATAACCTTTA TCTAAATAGAATACATTTAACCTGTCAATTATATGTATAGATTTATCCTAATTGGTTCAACATATATATG AATGCTTGTAATAGTCTGTTACTATTTGATAATGCATAAATGTTATATTTATATACAGTTACAGCAGGCC AGAAACATACATACACTGCATCCTGTAAGGTCTGTAAATCAGGGCTTCGGCAATGCCATTTTGCTATAGA CTCATTCATGTTTTTTAGGAATCAGAATCATTTCAGGAGAAAACCAACTAAGTAGACTTTAATTCTTCTC TTTCTCATTCTTGCCCTTTGAGAAACCCAAGTGGAATCATCACTTAATTACTTACTCAAATTCAATGAAT CAAATCCAATCCAATCAGTGAGAAGAATAGCTTATCTTTCAAGGTAATACTTTACCTTAGATCTTTGAGG GCATGCTTCCCATACAAAGAGCATCAGAAAATCAGGGCAATCATCTAGATCTCACAGATCTTCAACATAG GACTCTAGGTTATGAGAGCTGAACCACATAGCTATCAGTTTCAGTTTTTACTTCCTATTGAGTGTGTAGA AACACAGCCTATGGTTTTGCTGTCTGCAGAAGTTACCATACCCTGTAAAAGACAAATATTTCTTTCTCCT CTCGAACCACTAGTCAGCCTGGATATTCATGAAAGCTGAAAGAAGGGGTGCCAAAATGCCTAGCTCCTGG ATTTAGACTCATGGAATATGAGAAAGGCTTAGGGAAAATTCAAGTCATGGGGAGATTCCTGCTAGGTGAT GCCAGGAATCTCAATGACCTGTTGATTAGAGGGTCTCTGGTCATTTCCACTTGTGTGTGGAGAAATACAT CATTTGCATATGTATCTAGTCAAGAAGCCCTTTTGGAGCAAAGTGGTTGGGAAACTCATTTACTGGGTCC ACCAAAGGAAACCCAATTCTGCCATGTTTTTGCAAATACTACACGTCCCTTTGCTCTCAATAGCAAAAGT GATGTTATAGTTTTTTTTACTTTTGTCAGAATCAAGGCTTCGGCCACCTGTGCAATATTTTCCCATGGAA TGCAGCAGTAAATCCAAGTAGGTATATCCGTGCTCAGAGAGAATTCTCCATAGTTCTGACTCCTGAAAAA CAAGTCTCATTAGCTCTCATCTAGTTTGATGACTAAAAGTGCACAGCAGTTATACCCCAGTGGAAGTGAT TTTTCTCACCTAGCTGCTCTCTATCTCTTACGGCTATCTAGTTGTCTGAGCTGCTGGTTTGTTTTACTTA TTCTCCAACTGAACTCATTCCAAACCAAGAAAAAGGACCAAATGAAATTTTCAGTTATTATCATACTTGC TCCTTGAATAAGAATGCTAGAGTCTGGAGGCAGAAGAAGACTAAGTT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK090810 unedited TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGAAAAGCCTAGCTCCTG GATTTTGGCACCCCTTCTTTCAGCTTTCATGAATATCCAGGCTGACTAGTGGTTCGAGAG GAGAAAGAAAGGGTGCCAAAATGCCTAGCTCCTGGATTTAGACTCATGGAATATGAGAAA GGCTTAGGGAAAATTCAAGTCATGGGGAGATTCCTGCTAGGTGATGCCAGGAATCTCAAT GACCTGTTGATTAGAGGGTCTCTGGTCATTTCCACTTGTGTGTGGAGAAATACATCATTT GCATATGTATCTAGTCAAGAAGCCCTTTTGGAGCAAAGTGGTTGGGAAACTCATTTACTG GGTCCACCAAAGGAAACCCAATTCTGCCATGTTTTTGCAAATACTACACGTCCCTTTGCT CTCAATAGCAAAAGTGATGTTATAGTTTTTTTTACTTTTGTCAGAATCAAGGCTTCGGCC ACCTGTGCAATATTTTCCCATGGAATGCAGCAGTAAATCCAAGTAGGTATATCCCGTGCT CAGAGAGAATTCTCCATAGTTCTGACTCCTGAAAAACAAGTCTCATTAGCTCTCATCTAG TTTGATGACTAAAAGTGCACAGCAGTTATACCCCAGTGGAAGTGATTTTTCTCACCTAGC TGCTCTCTAACTCTTACGGCTATCTAGTTGTCTGAGCTGCTGGTTTGTTTTACTTATTCT NCAAACTGACTCATTCCANACCAAGAAAAAAGACCAATGAAATNNTCAGTTTTATCATAC TTGCTCCTTNNGATAAATGCTAGAGTCTGGAGCAGAAAAGACTAGTTAANAAAAACACCT GAAGATTCTGNAGTGAGTTTTGTAGTCTAATGCAAGAGAAAATC
Restriction Sites	NotI-NotI
ACCN	AK090810
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK090810.1
RefSeq Size	2077 bp
RefSeq ORF	2077 bp
Locus ID	6506
Protein Families	Transmembrane
Protein Pathways	Amyotrophic lateral sclerosis (ALS)
Gene Summary	This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Improper regulation of this gene is thought to be associated with several neurological disorders. Alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jun 2017]

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