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PHGDH (NM_006623) Human Untagged Clone
CAT＃: SC127007
PHGDH (untagged)-Human phosphoglycerate dehydrogenase (PHGDH)
CNY 5,960.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | 3-PGDH; 3PGDH; HEL-S-113; NLS; NLS1; PDG; PGAD; PGD; PGDH; PHGDHD; SERA |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC127007 sequence for NM_006623 edited (data generated by NextGen Sequencing)
ATGGCTTTTGCAAATCTGCGGAAAGTGCTCATCAGTGACAGCCTGGACCCTTGCTGCCGG AAGATCTTGCAAGATGGAGGGCTGCAGGTGGTGGAAAAGCAGAACCTTAGCAAAGAGGAG CTGATAGCGGAGCTGCAGGACTGTGAAGGCCTTATTGTTCGCTCTGCCACCAAGGTGACC GCTGATGTCATCAACGCAGCTGAGAAACTCCAGGTGGTGGGCAGGGCTGGCACAGGTGTG GACAATGTGGATCTGGAGGCCGCAACAAGGAAGGGCATCTTGGTTATGAACACCCCCAAT GGGAACAGCCTCAGTGCCGCAGAACTCACTTGTGGAATGATCATGTGCCTGGCCAGGCAG ATTCCCCAGGCGACGGCTTCGATGAAGGACGGCAAATGGGAGCGGAAGAAGTTCATGGGA ACAGAGCTGAATGGAAAGACCCTGGGAATTCTTGGCCTGGGCAGGATTGGGAGAGAGGTA GCTACCCGGATGCAGTCCTTTGGGATGAAGACTATAGGGTATGACCCCATCATTTCCCCA GAGGTCTCGGCCTCCTTTGGTGTTCAGCAGCTGCCCCTGGAGGAGATCTGGCCTCTCTGT GATTTCATCACTGTGCACACTCCTCTCCTGCCCTCCACGACAGGCTTGCTGAATGACAAC ACCTTTGCCCAGTGCAAGAAGGGGGTGCGTGTGGTGAACTGTGCCCGTGGAGGGATCGTG GACGAAGGCGCCCTGCTCCGGGCCCTGCAGTCTGGCCAGTGTGCCGGGGCTGCACTGGAC GTGTTTACGGAAGAGCCGCCACGGGACCGGGCCTTGGTGGACCATGAGAATGTCATCAGC TGTCCCCACCTGGGTGCCAGCACCAAGGAGGCTCAGAGCCGCTGTGGGGAGGAAATTGCT GTTCAGTTCGTGGACATGGTGAAGGGGAAATCTCTCACGGGGGTTGTGAATGCCCAGGCC CTTACCAGTGCCTTCTCTCCACACACCAAGCCTTGGATTGGTCTGGCAGAAGCTCTGGGG ACACTGATGCGAGCCTGGGCTGGGTCCCCCAAAGGGACCATCCAGGTGATAACACAGGGA ACATCCCTGAAGAATGCTGGGAACTGCCTAAGCCCCGCAGTCATTGTCGGCCTCCTGAAA GAGGCTTCCAAGCAGGCGGATGTGAACTTGGTGAACGCTAAGCTGCTGGTGAAAGAGGCT GGCCTCAATGTCACCACCTCCCACAGCCCTGCTGCACCAGGGGAGCAAGGCTTCGGGGAA TGCCTCCTGGCCGTGGCCCTGGCAGGCGCCCCTTACCAGGCTGTGGGCTTGGTCCAAGGC ACTACACCTGTACTGCAGGGGCTCAATGGAGCTGTCTTCAGGCCAGAAGTGCCTCTCCGC AGGGACCTGCCCCTGCTCCTATTCCGGACTCAGACCTCTGACCCTGCAATGCTGCCTACC ATGATTGGCCTCCTGGCAGAGGCAGGCGTGCGGCTGCTGTCCTACCAGACTTCACTGGTG TCAGATGGGGAGACCTGGCACGTCATGGGCATCTCCTCCTTGCTGCCCAGCCTGGAAGCG TGGAAGCAGCATGTGACTGAAGCCTTCCAGTTCCACTTCTAA Clone variation with respect to NM_006623.3 1326 g=>a >OriGene 5' read for NM_006623 unedited
AGCCTACGATTCACTATAGGGACGGCCGCGGNGCCGAACCGCAGCTTCTTGGCTTAGGTA CTTCTACNACAGCGGCCGATTCCGAGGCCAACTCCAGCAATGGCTTTTGCAAATCTGCGG AAAGTGCTCATCAGTGACAGCCTGGACCCTTGCTGCCGGAAGATCTTGCAAGATGGAGGG CTGCAGGTGGTGGAAAAGCAGAACCTTATCAAAGAGGAGCTGATAGCGGAGCTGCAGGAC TGTGAAGGCCTTATTGTTCGCTCTGCCACCAAGGTGACCGCTGATGTCATCAACGCAGCT GAGAAACTCCAGGTGGTGGGCAGGGCTGGCACAGGTGTGGACAATGTGGATCTGGAGGCC GCAACAAGGAAGGGCATCTTGGTTATGAACACCCCCAATGGGAACAGCCTCAGTGCCGCA GAACTCACTTGTGGAATGATCATGTGCCTGGCCAGGCAGATTCCCCAGGCGACGGCTTCG ATGAAGGACGGCAAATGGGAGCGGAAGAAGTTCATGGGAACAGAGCTGAATGGAAAGACC CTGGGAATTCTTGGCCTGNGCAGGATTGGGAGAGAGGTAGCTACCCGGATGCAGTCCTTT GGGATGAAGACTATAGGGTATGACCCCATCATTTCCCCAGAGGTCTCGGCCTCCTTTGGT GTTCAGCAGCTGCCCCTGGAGGAGATCTGGCCTCTCTGTGATTTCATCACTGTGCACACT CCTCTCCTGCCCTCCACGACAGGCTTGCTGAATGACAACACCTTTGCCCAGTGCAAGATG GGGTGCGTGTGGTGAACTGTGCCCGTGGAGGGATCGTGGACGAAGCGCCCTGCTCCGGCC CTGCAGTCTGCCAGTGTGCCGNGCTGACTGGACGTGNTTACGAAGAGCCGCACGGACCGG CCNNTGTGACATGAGATGTATCACTGTCA >OriGene 3' read for NM_006623 unedited
ATCGCTGCCGCAATTTANAGTCTAGTTATTAATCTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTATTGTTGTTTTTTTTTTTTTTTGGGGGGAGGCTTTTTATTAAACGGG TATTGGTGTACTACAGGGTCAAAGTGCAATGTAAACAGTGTCAAAAGCCCCCGTTCAACC CAAAAATGGGGATTTTCTCTCCCTATTGATCACAGGGGGGGGGTTTTTTTAAAAAAACCC CAAAAGCAGGGACCAATGAGCTCCAAGGTTAAAAATGGAACTGGAAGGGTTTAATCACAT GCTGGTTTCACGCTTTCAAGCTGGGCAACAAGGAGGAAATGCCCATGACGTGCCAAGTTT TCCCATTTGACACCAGTGAAATCTGGTAGGACAAGAGCCGCACGCCTGGCTTTGGCAGGA GGCCAATTATGGGAAGCAACATTGCAGGGTGAAAAGTCTTAATCCCGAATAAGAACAAGG GCCAGTCCCTGCGGAAAGGGCCTTTTTGGCTGAAAACAGCTTCCTTGAACCCCTGTAGAA CAAGTGTAGGGGCTTGGACCAAACCCCCAACCTGGTAAAGGGGCGCCTGCCAAGGCCACC GCCAAGAGGGATTCTCCCAAAGCCTTTTTTCCCTGGGGCAAAAGGGTTTTGGGAAGTGGT GACAATTAAGGCCGCTTCTTTTAACACCAGCTTAACGTTTACAAGATTTACAATCCCCTT GGTTGAGAACCCTTTTTCAGGAGGGCCGACAAATACTTGTGGGGGGTTATAAAAGTTCCC AATATTTTTAAGGGGTGTCCCCGGGGTATAACCCGGGAGGGGCCCCTTTGGGGGAACCCA CCCCAACGCTCGCATAATTGTTCCCCAAGAATTTGTGGCCACAACCATCATAAGGCTTTG TGTTTTGGAGAAAAAGAAACTTTGATAGGCCTCTGGATATTACAACACCCCCCGAGAAGA ATTTCCCCTTCTCCAGTTCCCACACATAGACACAGAAATTTCTCCACCACAAGGTGCTTG AGCCN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006623 |
| Insert Size | 2000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006623.2, NP_006614.2 |
| RefSeq Size | 1968 bp |
| RefSeq ORF | 1602 bp |
| Locus ID | 26227 |
| UniProt ID | O43175 |
| Domains | 2-Hacid_DH, 2-Hacid_DH_C |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Protein Pathways | Glycine, serine and threonine metabolism, Metabolic pathways |
| Gene Summary | This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known. [provided by RefSeq, Aug 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203949 | PHGDH (Myc-DDK-tagged)-Human phosphoglycerate dehydrogenase (PHGDH) |
CNY 5,952.00 |
|
| RC203949L1 | Lenti ORF clone of Human phosphoglycerate dehydrogenase (PHGDH), Myc-DDK-tagged |
CNY 8,352.00 |
|
| RC203949L2 | Lenti ORF clone of Human phosphoglycerate dehydrogenase (PHGDH), mGFP tagged |
CNY 8,352.00 |
|
| RC203949L3 | Lenti ORF clone of Human phosphoglycerate dehydrogenase (PHGDH), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203949L4 | Lenti ORF clone of Human phosphoglycerate dehydrogenase (PHGDH), mGFP tagged |
CNY 8,352.00 |
|
| RG203949 | PHGDH (tGFP-tagged) - Human phosphoglycerate dehydrogenase (PHGDH) |
CNY 7,552.00 |
|
| SC322244 | PHGDH (untagged)-Human phosphoglycerate dehydrogenase (PHGDH) |
CNY 5,960.00 |
