CD48 (BC030224) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BCM1; BLAST; BLAST1; CD48 antigen (B-cell membrane protein); CD48 molecule; hCD48; mCD48; MEM-102; OTTHUMP00000025680; OTTHUMP00000060268; SLAMF2
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC126734 sequence for BC030224 edited (data generated by NextGen Sequencing) ATGTGCTCCAGAGGTTGGGATTCGTGTCTGGCTCTGGAATTGCTACTGCTGCCTCTGTCA CTCCTGGTGACCAGCATTCAAGGTCACTTGGTACATATGACCGTGGTCTCCGGCAGCAAC GTGACTCTGAACATCTCTGAGAGCCTGCCTGAGAACTACAAACAACTAACCTGGTTTTAT ACTTTCGACCAGAAGATTGTAGAATGGGATTCCAGAAAATCTAAGTACTTTGAATCCAAA TTTAAAGGCAGGGTCAGACTTGATCCTCAGAGTGGCGCACTGTACATCTCTAAGGTCCAG AAAGAGGACAACAGCACCTACATCATGAGGGTGTTGAAAAAGACTGGGAATGAGCAAGAA TGGAAGATCAAGCTGCAAGTGCTTGGTGAGTCAGGGGAGCCAAAGAGCAAATCCCCACTC CAGTGGCCCCAGATGGACCACTGTAGGGCTTCCTGGGAGGCATGGGGCACTCTCGGGGAG GAGGAGAGAAAAACCTCAGGACAGGTTTGA Clone variation with respect to BC030224.1 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC030224 unedited GTATTTTGTAATACGACTCACTATAGGGCGGCCCGCGAATCGGCACGAGGCCTCGTGCCG AATTCGGCACGAGGACTGTCTCCTGCGTTGCTGGGAAGTTCTGGAAGGAAGCATGTGCTC CAGAGGTTGGGATTCGTGTCTGGCTCTGGAATTGCTACTGCTGCCTCTGTCACTCCTGGT GACCAGCATTCAAGGTCACTTGGTACATATGACCGTGGTCTCCGGCAGCAACGTGACTCT GAACATCTCTGAGAGCCTGCCTGAGAACTACAAACAACTAACCTGGTTTTATACTTTCGA CCAGAAGATTGTAGAATGGGATTCCAGAAAATCTAAGTACTTTGAATCCAAATTTAAAGG CAGGGTCAGACTTGATCCTCAGAGTGGCGCACTGTACATCTCTAAGGTCCAGAAAGAGGA CAACAGCACCTACATCATGAGGGTGTTGAAAAAGACTGGGAATGAGCAAGAATGGAAGAT CAAGCTGCAAGTGCTTGGTGAGTCAGGGGAGCCAAAGAGCAAATCCCCACTCCAGTGGCC CCAGATGGACCACTGTAGGGCTTCCTGGGAGGCATGGGGCACTCTCGGGGAGGAGGAGAG AAAAACCTCAGGACAGGTTTGATTCATTTTTCCTTGATCCAATTCTATTTGGGAGTGAGC CTAGGTCAACAGATATCCAAATTAACAATCTTAGGACATTTAACACCTCTTCTGAAGTTA GTGAGTCTAAGCTTTGAAGAGAGGAAAACTAGAGATTTTCCTATTTCCTTCATTNTGNCC TGTGATCAACCTAAATCAGCTCAGCTCATCTTGATTATATCTCCGAAATTATGCTTCATT GGACTTAAGAAAAAAGANATGTTTT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC030224 unedited TGGCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAATCTTGTAGAGCT TGTTAACCAGAACACCACTGTAAGAGACCTTGAATCTTTTTGTTCCCAGGACTTTCTGAA TTTTCAGCCCATATGACATGGAAGAAGAGTTAGAAGGGGAATCTGACTGCTTGGATCAGG GACTGTCTGGCTGGTGGAACTTGAATGTATACAACACTCTTCATTTGCATGTTTTGACAT TTTAAAAAATGTTTTCACTTTCCTTATCTACTTTGACTCTAATAACATCCTGAAAGGTAA GTAGAATTATTCTCATAATATGATGAAGAACCCAAGGCTCTCAAGGGAACACCACTCTTG GATTATTTAGCCCCGCTCTTCTACAAGAATTGCATACCCCAACCCCTGGAGCCCCTCCCC ATATCCATGTTTCTGCCCTATAATCTGATCCTTTCACCAAAAATGTTTAGATCAGGTACA CACACCTGAACTAAATTGGGACTATCAGTTTCTCTCTCTTTAAATTGCCCTGAGAATTGA AACTCAGAGGAGCAAAAGTCTAAGTTTTGAGAGATAACATGTGTAAAATGGCAGGAATCC AAATCCATAGGCACAGTCCACAGATTCCTGAAGCTAGGGGTTCTGGCCCGAACCTGGTTT TCAATCTTCTCCCCTTAAGCCCTTCCTAGCTCTTTTAAACTCTNTGAATTGCTTTANATA GCCCTAGTTTCTTAAACTAAATTCTCCTTTTTTGGGTAAGGTACATTGAATTAGTTTCTG TTATTTGCTATCAAAAGAATCTTACCTAATATGGGCACAGAGATGTGAAGATCTTTNTTC AGGCTGCCAGCTAGTGAGCACTGACCAGNTNTGACCCCAGTCTGTCTGGCTCCCAGTATA TGCCTGAGTCCTATTCCTATACCCTGGTAGACTGGAGCTTGANTGGAAGTTTTTTGTCCT GCCATNATGAAAGTGAAAAATCTCTGATACTCTGCC
Restriction Sites	NotI-NotI
ACCN	BC030224
Insert Size	1750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC030224.1, AAH30224.1
RefSeq Size	1806 bp
RefSeq ORF	1806 bp
Locus ID	962
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Natural killer cell mediated cytotoxicity
Gene Summary	This gene encodes a member of the CD2 subfamily of immunoglobulin-like receptors which includes SLAM (signaling lymphocyte activation molecules) proteins. The encoded protein is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

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