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PFKL (BC008964) Human Untagged Clone
CAT＃: SC126025
PFKL (untagged)-Human phosphofructokinase, liver, mRNA (cDNA clone MGC:4256 IMAGE:3029577), complete cds
CNY 4,928.00
货期*
现货
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | 6-phosphofructokinase, liver type; DKFZp686G1648; DKFZp686L2097; FLJ30173; FLJ40909; liver-type 1-phosphofructokinase; liver phosphofructokinase; PFK-B; phosphofructo-1-kinase isozyme B; phosphofructokinase, liver; phosphofructokinase 1; phosphohexokinase |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for BC008964 edited
CCCGTGTTTCGGCCGCCGCCATGGCCGCGGTGGACCTGGAGAAGCTGCGGGCGTCGGGCG CGGGCAAGGCCATCGGCGTCCTGACCAGCGGCGGCGACGCGCAAGGCATGAACGCTGCTG TCCGGGCTGTGACGCGCATGGGCATTTATGTGGGTGCCAAAGTCTTCCTCATCTACGAGG GCTATGAGGGCCTCGTGGAGGGAGGTGAGAACATCAAGCAGGCCAACTGGCTGAGCGTCT CCAACATCATCCAGCTGGGCGGCACTATCATTGGCAGCGCTCGCTGCAAGGCCTTTACCA CCAGGGAGGGGCGCCGGGCAGCGGCCTACAACCTGGTCCAGCACGGCATCACCAACCTGT GCGTCATCGGCGGGGATGGCAGCCTTACAGGTGCCAACATCTTCCGCAGCGAGTGGGGCA GCCTGCTGGAGGAGCTGGTGGCGGAAGGTAAGATCTCAGAGACTACAGCCCGGACCTACT CGCACCTGAACATCGCGGGCCTAGTGGGCTCCATCGATAACGACTTCTGCGGCACCGACA TGACCATCGGCACGGACTCGGCCCTCCACCGCATCATGGAGGTCATCGATGCCATCACCA CCACTGCCCAGAGCCACCAGAGGACCTTCGTGCTGGAAGTGATGGGCCGGCACTGCGGGT ACCTGGCGCTGGTATCTGCACTGGCCTCAGGGGCCGACTGGCTGTTCATCCCCGAGGCTC CACCCGAGGACGGCTGGGAGAACTTCATGTGTGAGAGGCTGGGTGAGACTCGGAGCCGTG GGTCCCGACTGAACATCATCATCATCGCTGAGGGTGCCATTGACCGCAACGGGAAGCCCA TCTCGTCCAGCTACGTGAAGGACCTGGTGGTTCAGAGGCTGGGCTTCGACACCCGTGTAA CTGTGCTGGGCCACGTGCAGCGGGGAGGGACGCCCTCTGCCTTCGACCGGATCCTGAGCA GCAAGATGGGCATGGAGGCGGTGATGGCGCTGCTGGAAGCCACGCCTGACACGCCGGCCT GCGTGGTCACCCTCTCGGGGAACCAGTCAGTGCGGCTGCCCCTCATGGAGTGCGTGCAGA TGACCAAGGAAGTGCAGAAAGCCATGGATGACAAGAGGTTTGACGAGGCCACCCAGCTCC GTGGTGGGAGCTTCGAGAACAACTGGAACATTTACAAGCTCCTCGCCCACCAGAAGCCCC CCAAGGAGAAGTCTAACTTCTCCCTGGCCATCCTGAATGTGGGGGCCCCGGCGGCTGGCA TGAATGCGGCCGTGCGCTCGGCGGTGCGGACCGGCATCTCCCATGGACACACAGTATACG TGGTGCACGATGGCTTCGAAGGCCTAGCCAAGGGTCAGGTGCAAGAAGTAGGCTGGCACG ACGTGGCCGGCTGGTTGGGGCGTGGTGGCTCCATGCTGGGGACCAAGAGGACCCTGCCCA AGGGCCAGCTGGAGTCCATTGTGGAGAACATCCGCATCTATGGTATTCACGCCCTGCTGG TGGTCGGTGGGTTTGAGGCCTATGAAGGGGTGCTGCAGCTGGTGGAGGCTCGCGGGCGCT ACGAGGAGCTCTGCATCGTCATGTGTGTCATCCCAGCCACCATCAGCAACAACGTCCCTG GCACCGACTTCAGCCTGGGCTCCGACACTGCTGTAAATGCCGCCATGGAGAGCTGTGACC GCATCAAACAGTCTGCCTCGGGGACCAAGCGCCGTGTGTTCATCGTGGAGACCATGGGGG GTTACTGTGGCTACCTGGCCACCGTGACTGGCATTGCTGTGGGGGCCGACGCCGCCTACG TCTTCGAGGACCCTTTCAACATCCACGACTTAAAGGTCAACGTGGAGCACATGACGGAGA AGATGAAGACAGACATTCAGAGGGGCCTGGTGCTGCGGAACGAGAAGTGCCATGACTACT ACACCACGGAGTTCCTGTACAACCTGTACTCATCAGAGGGCAACGGCGTCTTCGACTGCA GGACCAATGTCCTGGGCCACCTGCAGCAGGGTGGCGCTCCAACCCCCTTTGACCGGAACT ATGGGACCAAGCTGGGGGTGAAGGCCATGCTGTGGTTGTCGGAGAAGCTGCGCGAGGTTT ACCGCAAGGGACGGGTGTTCGCCAATGCCCCAGACTCGGCCTGCGTGATCGGCCTGAAGA AGAAGGCGGCGGCCTTCAGCCCCGTCACTGAGCTCAAGAAAGACACTGATTTCGAGCACC GCATGCCACGGGAGCAGTGGTGGCTGAGCCTGCGGCTCATGCTGAAGATGCTGGCACAAT ACCGCATCAGTATGGCCGCCTACGTGTCAGGGGAGCTGGAGCACGTGACCCGCCGCACCC TGAGCATGGACAAGGGCTTCTGAGGCCAGCCATGCCCACGCCCCTCCCCAGCCCCCACCC ATGCCAGCGCAGCGCCAGGGCTCAGATGGGGCCTGGGCTGTTGTGTCTGGAGCCTGCAGG CAGGTGGGGGCTGCGTCCCTGCTCAGCCCATCCCCTGCCTCTATCCCTGGCCACCTGCCA GGCCTCCCTCCGGCTGGTGTCTTGAGACCAGCCTGCCAGGCCCTCCAGCAGGAGGACAGA GTGCCCTGGGGCATCCACCTTCCTGCCCAGGGGACGTGGCGCTGTCGGTGTTTGGAGGCT GCTGCCCCCTGGCTTTGGCGCCCCATGGGCCCTCAGCGTCTCCCCATGCTGGGCTCACTA CATGGGCCAGCCCTTGCTCTACCTGGCCGGTAGGCTGCTGGCGCCTAGGTTGTGTTGAGA GGGGGATGCCCCTGGCCCTGCCTCACTGTGACCTGCTCCTGCCCACGTGCAGCACCTGTC ACCTTTTCTAGAAATAAAATCACCCTGACTGTGGGGTGCATCGGTCTCCGGAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA >OriGene 5' read for BC008964 unedited
GTACAGAATTGTAAACGACTCACTATAGGCGGCCGCGTAATTCGCACGAGGCCCGTGTTT CGGCCGCCGCCATGGCCGCGGTGGACCTGGAGAAGCTGCGGGCGTCGGGCGCGGGCAAGG CCATCGGCGTCCTGACCAGCGGCGGCGACGCGCAAGGCATGAACGCTGCTGTCCGGGCTG TGACGCGCATGGGCATTTATGTGGGTGCCAAAGTCTTCCTCATCTACGAGGGCTATGAGG GCCTCGTGGAGGGAGGTGAGAACATCAAGCAGGCCAACTGGCTGAGCGTCTCCAACATCA TCCAGCTGGGCGGCACTATCATTGGCAGCGCTCGCTGCAAGGCCTTTACCACCAGGGAGG GGCGCCGGGCAGCGGCCTACAACCTGGTCCAGCACGGCATCACCAACCTGTGCGTCATCG GCGGGGATGGCAGCCTTACAGGTGCCAACATCTTCCGCAGCGAGTGGGGCANCCTGCTGG AGGAGCTGGTGGCGGAAGGTAAGATCTCAGAGACTACAGCCCGGACCTACTCGCACCTGA ACATCGCGGGCCTAGTGGGCTCCATCGATAACGACTTCTGCGGCACCGACATGACCATCG GCACGGACTCGGCCCTCCACCGCATCATGGAGGTCATCGATGCCATCACCACCACTGCCC AGAGCCACCAGAGGACCTTCGTGCTGGAAGTGATGGGCCGGCACTGCGGGTACCTGGCGC TGGTATCTGCACTGGCCTCAGGGGCCGACTGGCTGTTCATCCCCGAGGCTCCACCCGAGA CGGCTGGNAGAACTCATGTGTGAGAGGCTGGGTGAGACTCGGAGCCGTGGGTCCCGACTG AACATCATCATCATCGCTGAGGGTGCCATTGACCGCAACGGAAGCCCATCTCGTCCAGCT ACTGAAAGACCTGTGGGTTCAGAGGCTGGGCTCCACACCGTGTAT |
| Restriction Sites | NotI-NotI |
| ACCN | BC008964 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC008964.2, AAH08964.1 |
| RefSeq Size | 2910 bp |
| Locus ID | 5211 |
| Protein Families | Druggable Genome |
| Protein Pathways | Fructose and mannose metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway |
| Gene Summary | This gene encodes the liver (L) subunit of an enzyme that catalyzes the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate, which is a key step in glucose metabolism (glycolysis). This enzyme is a tetramer that may be composed of different subunits encoded by distinct genes in different tissues. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014] |
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