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BCL2 (NM_000633) Human Untagged Clone
CAT＃: SC125546
BCL2 (untagged)-Human B-cell CLL/lymphoma 2 (BCL2), nuclear gene encoding mitochondrial protein, transcript variant alpha
CNY 3,600.00
| Cited in 10 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | Bcl-2; PPP1R50 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_000633, the custom clone sequence may differ by one or more nucleotides
GAATTCCCGGGATATCGTCGACCCACGCGTCCGCTGGAGAGTGCTGAAGATTGATGGGATCGTTGCCTTA TGCATTTGTTTTGGTTTTACAAAAAGGAAACTTGACAGAGGATCATGCTGTACTTAAAAAATACAACATC ACAGAGGAAGTAGACTGATATTAACAATACTTACTAATAATAACGTGCCTCATGAAATAAAGATCCGAAA GGAATTGGAATAAAAATTTCCTGCATCTCATGCCAAGGGGGAAACACCAGAATCAAGTGTTCCGCGTGAT TGAAGACACCCCCTCGTCCAAGAATGCAAAGCACATCCAATAAAATAGCTGGATTATAACTCCTCTTCTT TCTCTGGGGGCCGTGGGGTGGGAGCTGGGGCGAGAGGTGCCGTTGGCCCCCGTTGCTTTTCCTCTGGGAA GGATGGCGCACGCTGGGAGAACGGGGTACGATAACCGGGAGATAGTGATGAAGTACATCCATTATAAGCT GTCGCAGAGGGGCTACGAGTGGGATGCGGGAGATGTGGGCGCCGCGCCCCCGGGGGCCGCCCCCGCACCG GGCATCTTCTCCTCCCAGCCCGGGCACACGCCCCATCCAGCCGCATCCCGGGACCCGGTCGCCAGGACCT CGCCGCTGCAGACCCCGGCTGCCCCCGGCGCCGCCGCGGGGCCTGCGCTCAGCCCGGTGCCACCTGTGGT CCACCTGACCCTCCGCCAGGCCGGCGACGACTTCTCCCGCCGCTACCGCCGCGACTTCGCCGAGATGTCC AGCCAGCTGCACCTGACGCCCTTCACCGCGCGGGGACGCTTTGCCACGGTGGTGGAGGAGCTCTTCAGGG ACGGGGTGAACTGGGGGAGGATTGTGGCCTTCTTTGAGTTCGGTGGGGTCATGTGTGTGGAGAGCGTCAA CCGGGAGATGTCGCCCCTGGTGGACAACATCGCCCTGTGGATGACTGAGTACCTGAACCGGCACCTGCAC ACCTGGATCCAGGATAACGGAGGCTGGGATGCCTTTGTGGAACTGTACGGCCCCAGCATGCGGCCTCTGT TTGATTTCTCCTGGCTGTCTCTGAAGACTCTGCTCAGTTTGGCCCTGGTGGGAGCTTGCATCACCCTGGG TGCCTATCTGGGCCACAAGTGAAGTCAACATGCCTGCCCCAAACAAATATGCAAAAGGTTCACTAAAGCA GTAGAAATAATATGCATTGTCAGTGATGTACCATGAAACAAAGCTGCAGGCTGTTTAAGAAAAAATAACA CACATATAAACATCACACACACAGACAGACACACACACACACAACAATTAACAGTCTTCAGGCAAAACGT CGAATCAGCTATTTACTGCCAAAGGGAAA >OriGene 5' read for NM_000633 unedited
NGTTCACGTTAAAATTTGTAATAGACTCACTATAGGCGGCCGCGNAATCAGATCTGGTAC CGGTCCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGCTGGAGAGTGCTGAAGATTG ATGGGATCGTTGCCTTATGCATTTGTTTTGGTTTTACAAAAAGGAAACTTGACAGAGGAT TCATGCTGTACTTAAAAAATACAACATCACAGAGGAAGTAGACTGATATTAACAATACTT ACTAATAATAACGTGCCTCATGAAATAAAGATCCGAAAGGAATTGGAATAAAAATTTCCT GCATCTCATGCCAAGGGGGAAACACCAGAATCAAGTGTTCCGCGTGATTGAAGACACCCC CTCGTCCAAGAATGCAAAGCACATCCAATAAAATAGCTGGATTATAACTCCTCTTCTTTC TCTGGGGGCCGTGGGGTGGGAGCTGGGGCGAGAGGTGCCGTTGGCCCCCGTTGCTTTTCC TCTGGGAAGGATGGCGCACGCTGGGAGAACGGGGTACGATAACCGGGAGATAGTGATGAA GTACATCCATTATAAGCTGTCGCAGAGGGGCTACGAGTGGGATGCCGGAGAATGTTGGCG CCGCGCCCCCGGGGGCCGCCCCCGCACCGGGCATCTTCTCCTCCCAGCCCGGGCACACGC CCCATCCAGCCGCATCCCGGGACCCGGTCGCCAGGACCTCGCCGCTGCAGACCCCCGCTG CCCNCGGCGCGGCCGCGGGGGCCTGCGCTCAGCCCGGTGCCACCCTGTGTCCACCTGACC CTCGCCAGCCNGCGACGACTTCTCCGCCGCTACCGCCGCGACTTCGCCGAGATGTTCAGC CAGCTGCACCTGACGCCTTTACCGCGCCGGGGACGCTTTGCACCGTGGGGTGGAAGAGCT CTTCAGGNACGGGGTG >OriGene 3' read for NM_000633 unedited
CTGGGCCATTGGNGATGGCAACTTCCAGGNCCAGNAAAGCACTGGGGNAGGGTCACAGGN ATGCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCCCTTTTTTTTTTTTT TTAATGCCCCAGGATGTACAGATAACCCCCATATTCCACACCTGGAACTTTTTTTTTGTC AGGTTTTCAAATAAAACCAAACTACAGTGACAAGATAATGTTTTACATGTAATTCCATAG ACAGGGGTCAATTAATCCATGACACCTCACTTCAATGCTTCTTTTAGGATTTGTGACCCT CTCCAAAGTCATTTAAAGCCTTGCTTTAAACTCACAGGTGGGCCAAGGCCACACAGCCAA CGTGCCATGTGCTACAGCCAAAATGGGCCGTGGCCATTGCCTCTCCTCACGTTCCCAGCC TTCACCATGTCCTTCTGATAGGAAGGGCCCAGGGCCTCTGTTCCTTCCCTCTACAGTGAT ACATGTCTTAAGAAGGGTCGTGGCTCCCATGCTCCACGTGAAAACGGGCCTACCTGGAGG GCCCCAGGGTGACAGGCCCAGCCACACCCCTCTACTGCTCTTTCTTGACAGTGGAATTCT GAGCTCCATCAGCTTCCAGACATTCGGAGACCACACTGCCCTGTTGATCATCCCTGGAGG AGGCCAGTGAGGGCCCCGGCTCAGTTCCAGGACCAGGCCTCCAAGCTGGGACACAGGCAG GTTCTGCGGACTTCGGTCTCCTAAAACAGGCACTTGTGGCGGCCTGATGCTCTGGGTAAC TCTAGCCTTCCTGATGCGGAAGTCACCGAAATGTTCACTTCCTCAAGTTCCAGAGGATTC TGTTTCTTACTCAGACAGAGCCAGTATTGGGAGTTGGGGGGGGTGCGTATCCAAAATATA TGAATATACACAATCG |
| Restriction Sites | Please inquire |
| ACCN | NM_000633 |
| Insert Size | 3050 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000633.2, NP_000624.2 |
| RefSeq Size | 6492 bp |
| RefSeq ORF | 720 bp |
| Locus ID | 596 |
| UniProt ID | P10415 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transmembrane |
| Protein Pathways | Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Focal adhesion, Neurotrophin signaling pathway, Pathways in cancer, Prostate cancer, Small cell lung cancer |
| Gene Summary | This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016] Transcript Variant: This variant (alpha) represents the longer transcript and encodes the longer isoform (alpha). |
Citations (10)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Bcl-2 inhibitors enhance FGFR inhibitor-induced mitochondrial-dependent cell death in FGFR2 mutant endometrial cancer
,Packer, LM;Stehbens, SJ;Bonazzi, VF;Gunter, J;Ju, RJ;Ward, M;Gartside, M;Byron, S;Pollock, PM;,
Mol Oncol
,PubMed ID 30537101
[BCL2]
|
|
Prolonged engraftment of transplanted hepatocytes in the liver by transient pro-survival factor supplementation using ex vivo mRNA transfection
,Matsui, A;Uchida, S;Hayashi, A;Kataoka, K;Itaka, K;,
J Control Release
,PubMed ID 29966689
[BCL2]
|
|
Inhibition of Bcl-2 potentiates AZD-2014-induced anti-head and neck squamous cell carcinoma cell activity
,Li, Y;Cui, JT;,
Biochem. Biophys. Res. Commun.
,PubMed ID 27343560
[BCL2]
|
|
Apoptosis as the focus of an authentic research experience in a cell physiology laboratory
,Byrd, SK;,
Adv Physiol Educ
,PubMed ID 27231261
[BCL2]
|
|
Messenger RNA-based therapeutics for the treatment of apoptosis-associated diseases
,Matsui, A;Uchida, S;Ishii, T;Itaka, K;Kataoka, K;,
Sci Rep
,PubMed ID 26507781
[BCL2]
|
|
Sorafenib Sensitizes (–)-Gossypol-Induced Growth Suppression in Androgen-Independent Prostate Cancer Cells via Mcl-1 Inhibition and Bak Activation
,Jiqin Lian, Zhenhong Ni, Xufang Dai, Chang Su, Amber Rae Smith, Liang Xu, and Fengtian He,
Mol. Cancer Ther., Feb 2012; 11: 416 - 426.
[BCL2]
|
|
uPAR and Cathepsin B Downregulation Induces Apoptosis by Targeting Calcineurin A to BAD via Bcl-2 in Glioma
,null,
Journal of Neuro-Oncology
,PubMed ID 21964739
[BCL2]
|
|
A natural BH3 mimetic induces autophagy in apoptosis-resistant prostate cancer via modulating Bcl-2–Beclin1 interaction at endoplasmic reticulum
,J Lian, X Wu, F He, D Karnak, W Tang, Y Meng, D Xiang, M Ji, T S Lawrence, L Xu,
Cell Death and Differentiation (25 June 2010) doi:10.1038/cdd.2010.74 Original Paper
[BCL2]
|
|
BCL2 inhibits cell adhesion, spreading, and motility by enhancing actin polymerization
,Hengning Ke, Vandy I Parron, Jeff Reece, Jennifer Y Zhang, Steven K Akiyama, John E French,
Cell Research (9 February 2010) doi:10.1038/cr.2010.21 Original Article
[BCL2]
|
|
Mitogen-Activated Protein Kinase Inhibition Induces Translocation of Bmf to Promote Apoptosis in Melanoma
,Matthew W. VanBrocklin, Monique Verhaegen, Maria S. Soengas, and Sheri L. Holmen,
Cancer Res., Mar 2009; 69: 1985 - 1994.
[BCL2]
|
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