USP33 (AF383172) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	VDU1
Vector	pCMV6-XL6
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AF383172, the custom clone sequence may differ by one or more nucleotides ATGACAGGATCAAATTCACACATAACGATATTAACCTTAAAGGTGTTACCTCATTTTGAAAGTCTTGGGA AACAGGAAAAAATTCCTAACAAAATGTCAGCTTTTCGAAATCATTGTCCACATTTGGATTCAGTTGGTGA AATAACAAAAGAAGATTTGATACAAAAATCCCTTGGTACTTGTCAGGATTGTAAAGTCCAAGGACCAAAT CTTTGGGCATGTCTGGAGAATAGATGTTCATATGTTGGCTGTGGTGAATCACAAGTAGATCACAGCACCA TACATTCTCAGGAGACAAAGCATTATCTAACTGTGAACCTTACCACTCTTCGAGTATGGTGTTATGCTTG CAGCAAAGAAGTATTTTTGGATAGGAAATTAGGAACTCAGCCTTCATTGCCTCATGTAAGACAACCTCAC CAAATACAAGAAAACAGTGTCCAGGATTTTAAAATACCCAGTAATACAACATTAAAAACTCCTCTGGTTG CCGTATTTGATGATCTGGATATAGAAGCGGATGAAGAAGATGAACTTAGGGCCAGAGGTCTTACAGGTTT GAAAAATATTGGAAATACTTGTTACATGAATGCAGCTTTGCAGGCTCTTTCTAATTGCCCACCTTTGACA CAGTTTTTTCTTGATTGTGGAGGACTAGCTCGAACAGATAAGAAACCTGCCATTTGTAAAAGTTATCTCA AACTAATGACAGAGCTGTGGTATAAAAGCAGGCCAGGATCTGTTGTGCCTACTACTCTGTTTCAAGGAAT TAAAACTGTAAATCCAACATTTCGGGGGTATTCTCAGCAGGATGCTCAAGAATTCCTTCGATGTTTAATG GATTTGCTTCATGAAGAATTGAAAGAGCAAGTCATGGAAGTAGAAGAAGATCCGCAAACCATAACCACTG AGGAGACAATGGAAGAAGACAAGAGCCAGTCGGATGTAGATTTTCAGTCTTGTGAATCTTGTAGCAACAG TGATAGAGCAGAAAATGAAAATGGCTCTAGATGCTTTTCTGAAGATAATAATGAAACAACAATGTTAATT CAGGATGATGAAAACAATTCAGAAATGTCAAAGGATTGGCAAAAAGAGAAGATGTGCAATAAGATTAATA AAGTAAATTCTGAAGGCGAATTTGATAAAGATAGAGACTCTATATCTGAAACAGTCGACTTAAACAACCA GGAAACTGTCAAAGTGCAAATACACAGCAGAGCTTCAGAATATATCACTGATGTCCATTCGAATGACCTG TCTACACCACAGATCCTTCCATCAAATGAAGGTGTTAATCCACGTTTATCGGCAAGCCCTCCTAAATCAG GCAATTTGTGGCCAGGATTGGCACCACCACACAAAAAAGCTCAGTCTGCATCTCCAAAGAGAAAAAAACA GCACAAGAAATACAGAAGTGTTATTTCAGACATATTTGATGGAACAATCATTAGTTCAGTGCAGTGTCTG ACTTGTGACAGGGTGTCTGTAACCCTCGAGACCTTTCAAGATCTGTCCTTGCCAATTCCTGGCAAGGAAG ACCTTGCTAAGCTGCATTCATCAAGTCATCCAACTTCTATAGTCAAAGCAGGATCATGTGGCGAAGCATA TGCTCCACAAGGGTGGATAGCTTTTTTCATGGAATATGTGAAGAGGTTTGTTGTCTCATGTGTCCCTAGC TGGTTTTGGGGTCCAGTAGTAACCTTGCAAGATTGTCTTGCTGCCTTCTTTGCCAGAGATGAACTAAAAG GTGACAATATGTACAGTTGTGAAAAATGCAAAAAGTTGAGAAATGGAGTGAAGTTTTGTAAAGTACAAAA CTTTCCTGAGATTTTGTGCATCCACCTTAAAAGATTCAGACATGAACTAATGTTTTCCACCAAAATCAGT ACCCATGTTTCATTTCCGCTAGAAGGCTTGGATCTTCAGCCATTTCTTGCTAAGGATAGTCCAGCTCAAA TTGTGACATATGATCTTCTGTCAGTCATTTGCCATCATGGAACTGCAAGTAGTGGACACTATATAGCCTA CTGCCGAAACAATCTAAATAATCTCTGGTATGAATTTGATGATCAGAGTGTCACTGAAGTTTCAGAATCT ACTGTACAAAATGCAGAAGCTTACGTTCTTTTCTATAGGAAGAGCAGCGAAGAGGCACAAAAAGAGAGGA GAAGGATATCAAATTTATTGAACATAATGGAACCAAGCCTCCTTCAGTTTTATATTTCTCGACAGTGGCT TAATAAATTTAAGACCTTTGCCGAACCTGGCCCTATTTCAAATAATGACTTTCTTTGTATTCATGGAGGT GTTCCTCCAAGAAAAGCTGGTTATATTGAAGACCTGGTTTTGATGCTGCCTCAGAACATTTGGGATAACC TATATAGCAGGTATGGTGGAGGACCAGCTGTCAACCATCTGTACATTTGTCATACTTGCCAAATTGAGGC GGAGAAAATTGAAAAAAGAAGAAAAACTGAATTGGAAATTTTTATTCGGCTTAACAGAGCGTTCCAAAAA GAGGACTCTCCAGCTACTTTTTATTGCATCAGTATGCAGTGGTTTAGAGAATGGGAAAGTTTTGTGAAGG GTAAAGATGGAGATCCTCCAGGTCCTATTGACAATACTAAGATTGCAGTCACTAAATGTGGTAATGTGAT GCTTAGGCAAGGAGCAGATTCTGGCCAGATTTCTGAAGAAACATGGAATTTTCTGCAGTCTATTTATGGT GGAGGGCCTGAAGTTATCCTGCGACCTCCGGTTGTTCATGTTGATCCAGATATACTTCAAGCAGAAGAAA AAATTGAAGTAGAAACTCGGTCTTTGTAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF383172 unedited CTCATTACACCCGCCCGTTGNCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATAT AAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGC CGCGAATTCGGCACGAGGGGAAAAGAGGAGCTTGCGGAGGTGCGGCTGCAGGCCGTTGTT GGTCGAGCTGGCGGGTCCCGCGGGCCAGGCCGTGGAGAGCTCCTGAAGGAAGCACTAAAC ATGGAAAGGAACAACCGGTACCAGCCACTGCAAAAACATGCCAAATTGTAAAGACCATCG ATGTTAGGAAGAAACTGCCTCAACTAATGAGCAAAATAACCAGCTAACATCGTAATGACA GGATCAAATTCACACATAACGATATTAACCTTAAAGGTGTTACCTCATTTTGAAAGTCTT GGGAAACAGGAAAAAATTCCTAACAAAATGTCAGCTTTTCGAAATCATTGTCCACATTTG GATTCAGTTGGTGAAATAACAAAAGAAGATTTGATACAAAAATCCCTTGGTACTTGTCAG GATTGTAAAGTCCAAGGACCAAATCTTTGGGCATGTCTGGAGAATAGATGTTCATATGTT GGCTGTGGTGAATCACAAGTAGATCACAGCACCATACATTCTCAGGAGACANAGCATTAT CTAACTGTGAACCTTACCACTCTTCGAGTATGGTGTTATGCTTGCAGCANAGAAGTATTT TTGGATAGGAAATTAGGAACTCAGCCTTCATTGCCTCATGNTAGACAACCTCACCANNAT ACAGAAAACAGTGTCCACGGATTTTAANATACCCCAGTATACCACATTTAAAACTCCTCT GGTTGCCGTATTTGATGATCTGGATATAGAAGCGCATGAAGAAGATGAACTTAGGGCCAN AGTCTTACAGTTTTGAAAATATTGGAATACTTGTACATGAATGCAGCTTTG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AF383172 unedited ATTGATCATTGGGNGATGGTCAACTTNCCAGGNCCAGNAAAGCACTGGGGNAGGGAGTCA CAGGGCATGCCACCCGGGTTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAG TCGAGTTTTTTTTTTTTTTTTTTACATTTTAAAGACATTTTTATTGAGCTAATTTTAACA ACATTGCTTTAGCTGGTGACAGCTGCCCCAAACCAAAACAAAGCCATCATGAATGCTATT CAACATCCTCAATGTAATCCAGTATGTTTTTGTACTTGGAATATAGTTAAACTTTTGACA TTACATAATCAAGCAAATAGCAGTGCATACTATATTATTCAAAAAGACTTTATCTATTTC ATTTAAAAAATCAAGTTGCAAGTGGCCTCAGCTTTATCAACAATCGTAGTGACACATTCC ACACTTCATGCTCTCAAAATAAAAAGTGCCCTAAAACTAACTCTAAGTTTTTTAGTCACT GACATTAATACTAACCAGGTTACAGGAATTGAAGTTTAACATTGTACAATATAAGCGGCA ATAAGTTACTGATATCTGCTGACAAATTCCACTCAAACTAAATATATCCTTGATACATTC AAAAAGACATTTTGTAAATTTTAATAAGCTAGTTAGTGTATATGCTGCAGTGCAAAAAAA GTGACACCCTAGTTTCCTGAGCTTTAGGAAATTAACAATTTTCTGACTACTACTTTCAAC TTAAAATAGGCTCTTTATTATATGAAGCCATGACAAAACTATACAATTAAATTGGGCTTT TGGAGAACTGANAGACTGGTCAGAGTCAGGGTGATTTAGGACAATGAAAAAGTTTTCAAA GCAAAGTATTTACACACTNACAAGATACTATGGATCTGCATGTAGTGTAAAGATTAAAGA TACCTACTN
Restriction Sites	NotI-NotI
ACCN	AF383172
Insert Size	4700 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AF383172.1, AAL78314.1
RefSeq Size	4502 bp
RefSeq ORF	2829 bp
Locus ID	23032
Domains	UCH, zf-UBP, DUSP
Protein Families	Druggable Genome, Protease
Gene Summary	This gene encodes a deubiquinating enzyme important in a variety of processes, including Slit-dependent cell migration and beta-2 adrenergic receptor signaling. The protein is negatively regulated through ubiquitination by von Hippel-Lindau tumor protein (VHL). Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Jun 2012]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.