FECH (NM_000140) Human Untagged Clone

CAT#: SC125310

FECH (untagged)-Human ferrochelatase (FECH), nuclear gene encoding mitochondrial protein, transcript variant 2



  "NM_000140" in other vectors (6)

CNY 2,950.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
FECH Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms EPP; EPP1; FCE
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC125310 sequence for NM_000140 edited (data generated by NextGen Sequencing)
ATGCGTTCACTCGGCGCAAACATGGCTGCGGCCCTGCGCGCCGCGGGCGTCCTGCTCCGC
GATCCGCTGGCATCCAGCAGCTGGAGGGTCTGTCAGCCATGGAGGTGGAAGTCAGGTGCA
GCTGCAGCGGCCGTCACCACAGAAACAGCCCAGCATGCCCAGGGTGCAAAACCTCAAGTT
CAACCGCAGAAGAGGAAGCCGAAAACTGGAATATTAATGCTAAACATGGGAGGCCCTGAA
ACTCTTGGAGATGTTCACGACTTCCTTCTGAGACTCTTCTTGGACCGAGACCTCATGACA
CTTCCTATTCAGAATAAGCTGGCACCATTCATCGCCAAACGCCGAACCCCCAAGATTCAA
GAGCAGTACCGCAGGATTGGAGGCGGATCCCCCATCAAGATATGGACTTCCAAGCAGGGA
GAGGGCATGGTGAAGCTGCTGGATGAATTGTCCCCCAACACAGCCCCTCACAAATACTAT
ATTGGATTTCGGTACGTCCATCCTTTAACAGAAGAAGCAATTGAAGAGATGGAGAGAGAT
GGCCTAGAAAGGGCTATTGCTTTCACACAGTATCCACAGTACAGCTGCTCCACCACAGGC
AGCAGCTTAAATGCCATTTACAGATACTATAATCAAGTGGGACGGAAGCCCACGATGAAG
TGGAGCACTATTGACAGGTGGCCCACACATCACCTCCTCATCCAGTGCTTTGCAGATCAT
ATTCTAAAGGAACTGGACCATTTTCCACTTGAGAAGAGAAGCGAGGTGGTCATTCTGTTT
TCTGCTCACTCACTGCCCATGTCTGTGGTCAACAGAGGCGACCCATATCCTCAGGAGGTA
AGCGCCACTGTCCAAAAAGTCATGGAAAGGCTGGAGTACTGCAACCCCTACCGACTGGTG
TGGCAATCCAAGGTTGGTCCAATGCCCTGGTTGGGTCCTCAAACAGACGAATCTATCAAA
GGGCTTTGTGAGAGGGGGAGGAAGAATATCCTCTTGGTTCCGATAGCATTTACCAGTGAC
CATATTGAAACGCTGTATGAGCTGGACATCGAGTACTCTCAAGTTTTAGCCAAGGAGTGT
GGAGTTGAAAACATCAGAAGAGCTGAGTCTCTTAATGGAAATCCATTGTTCTCTAAGGCC
CTGGCCGACTTGGTGCATTCACACATCCAGTCAAACGAGCTGTGTTCCAAGCAGCTGACC
CTGAGCTGTCCGCTCTGTGTCAATCCTGTCTGCAGGGAGACTAAATCCTTCTTCACCAGC
CAGCAGCTGTGA

Clone variation with respect to NM_000140.3
>OriGene 5' read for NM_000140 unedited
TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAAT
TCGGCACGAGGGGAGCCCGGACTCGGGCCGAGGCTGCCCAGGCAATGCGTTCACTCGGCG
CAAACATGGCTGCGGCCCTGCGCGCCGCGGGCGTCCTGCTCCGCGATCCGCTGGCATCCA
GCAGCTGGAGGGTCTGTCAGCCATGGAGGTGGAAGTCAGGTGCAGCTGCAGCGGCCGTCA
CCACAGAAACAGCCCAGCATGCCCAGGGTGCAAAACCTCAAGTTCAACCGCAGAAGAGGA
AGCCGAAAACTGGAATATTAATGCTAAACATGGGAGGCCCTGAAACTCTTGGAGATGTTC
ACGACTTCCTTCTGAGACTCTTCTTGGACCGAGACCTCATGACACTTCCTATTCAGAATA
AGCTGGCACCATTCATCGCCAAACGCCGAACCCCCAAGATTCAAGAGCAGTACCGCAGGA
TTGGAGGCGGATCCCCCATCAAGATATGGACTTCCAAGCAGGGAGAGGGCATGGTGAAGC
TGCTGGATGAATTGTCCCCCAACACAGCCCCTCACAAATACTATATTGGATTTCGGTACG
TCCATCCTTTAACAGAAGAAGCAATTGAAGAGATGGAGAGAGATGGCCTAGAAAGGGCTA
TTGCTNTCACACAGTATCCACAGTACAGCTGCTCCACCACAGGCAGCAGCTTAAATGCCA
TTTACAGATACTATAATCAAGTGGGACGGAAGCCCACGATGAAGTGGAGCACTATTGACA
GGTGGGCCACACATNNACTNCTCATNCAGTGCTTTGCAGATCATATTCTAAAGAACTGGA
CATTTTNCACTTGAGAGAGAAGCGAGGTGTCATTCTGTTTT
>OriGene 3' read for NM_000140 unedited
CTATGGCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGACATTATTTCT
ATATTTTTAGAAACACTTTTCACTCATAAATCCAAGGTGACAATGGCATTAAATGGCATT
AAAAATATCTACAGGAAAGGACTACCACGAAATACAATATTATGATAACAGTTATTGCAT
TAAAGTGGTTACTTTTCTGTATTCTTCACATGTCCCATTGTATCACTATACATTTTTATA
ATAAAAAATCGTAAATTAAGAAAGAATTCAAGCAAAGTATTGTTGACTTTATAAAATAAT
TCTTAAAACAAATGTTCAGAAAGAAGGCATTCAACTAAGATAGAGAAGATGCTGGATGGA
GAGGCTTGCAAGGTCCCGGGAGGAGCTCCAGGGTGAAGCTCATCACTAGCCTTTCCAGCA
GCACTGTCCCTGGAGACCAGAAGCAGTGGGAGCCCCTTCCCTGTGGCACCACGGACTCCC
ACCAAGGCACGTATCATGAACACTGTACTTGTACACGTTTATTACCCTTGGGTGTATGGG
GAATCTAAGAGCACCTCTGAAATCCATACTTGATTCCTTTTTACATTGAGGAAAGTGAGA
TTCACACACAAATACCAAGATGACCAATGAATGGTGGCCTCGCTGGCAAAAGCAGCCATT
GCAGGTAACCAAATTGCCTCCCTAGCATTCTGTAACCCATGGGCTTAAGGAAAAATTAAG
TAACATTTCTTTTTCAAATTCATTTCAAAAACTCAACTGTGTTCATTTTAAAAACAGCAA
CTATATTGCCCGAAACTAAACAGATTTCATTCACACTGCCAGCCCACAGAGGGGACTCTC
CCTACCCTTTAAGAAGGGTTTTGGCCAATAAACCCAAAAT
Restriction Sites NotI-NotI     
ACCN NM_000140
Insert Size 2500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000140.2, NP_000131.2
RefSeq Size 3817 bp
RefSeq ORF 1272 bp
Locus ID 2235
UniProt ID P22830
Domains Ferrochelatase
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Porphyrin and chlorophyll metabolism
Gene Summary The protein encoded by this gene is localized to the mitochondrion, where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Mutations in this gene are associated with erythropoietic protoporphyria. Two transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene is found on chromosome 3.[provided by RefSeq, May 2010]
Transcript Variant: This variant (2) uses an alternate in-frame donor splice site at one of the internal coding exons, compared to transcript variant 1. The encoded isoform (b) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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