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CMH1 (MYH7) (NM_000257) Human Untagged Clone
CAT＃: SC125249
MYH7 (untagged)-Human myosin, heavy chain 7, cardiac muscle, beta (MYH7)
CNY 19,520.00
货期*
现货
规格
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经常一起买 (3)
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CMD1S; CMH1; MPD1; MYHCB; SPMD; SPMM |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC125249 sequence for NM_000257 edited (data generated by NextGen Sequencing)
ATGGGAGATTCGGAGATGGCAGTCTTTGGGGCTGCCGCCCCCTACCTGCGCAAGTCAGAG AAGGAGCGGCTAGAAGCGCAGACCAGGCCTTTTGACCTCAAGAAGGATGTCTTCGTGCCT GATGACAAACAGGAGTTTGTCAAGGCCAAGATCGTGTCTCGAGAGGGTGGCAAAGTCACT GCCGAGACTGAGTATGGCAAGACAGTGACCGTGAAGGAGGACCAGGTGATGCAGCAGAAC CCACCCAAGTTCGACAAAATCGAGGACATGGCCATGCTGACCTTCCTGCATGAGCCCGCG GTGCTCTACAACCTCAAGGATCGCTACGGCTCCTGGATGATCTACACCTACTCGGGCCTC TTCTGTGTCACCGTCAACCCTTACAAGTGGCTGCCGGTGTACACTCCTGAGGTGGTGGCT GCCTACCGGGGCAAGAAGAGGAGCGAGGCCCCGCCCCACATCTTCTCCATCTCCGACAAC GCCTATCAGTACATGCTGACAGACAGAGAAAACCAGTCCATCCTGATCACCGGAGAATCC GGAGCAGGGAAGACAGTCAACACCAAGAGGGTCATCCAGTACTTTGCTGTTATTGCAGCC ATTGGGGACCGCAGCAAGAAGGACCAGAGCCCGGGCAAGGGCACCCTGGAGGACCAGATC ATCCAGGCCAACCCTGCTCTGGAGGCCTTTGGCAATGCCAAGACCGTCCGGAACGACAAC TCCTCCCGCTTTGGGAAATTCATTCGAATTCATTTTGGGGCAACAGGAAAGTTGGCATCT GCAGACATAGAGACCTATCTTCTGGAAAAATCCAGAGTTATTTTCCAGCTGAAAGCAGAG AGAGATTATCACATTTTCTACCAAATCCTGTCTAACAAAAAGCCTGAGCTGCTGGACATG CTGCTGATCACCAACAACCCCTACGATTATGCATTCATCTCCCAAGGAGAGACCACCGTG GCCTCCATTGATGACGCTGAGGAGCTCATGGCCACTGATAACGCTTTTGATGTGCTGGGC TTCACTTCAGAGGAGAAAAACTCCATGTATAAGCTGACAGGTGCCATCATGCACTTTGGA AACATGAAGTTCAAGCTGAAGCAGCGGGAGGAGCAGGCGGAGCCAGACGGCACTGAAGAG GCTGACAAGTCTGCCTACCTCATGGGGCTGAACTCAGCCGACCTGCTCAAGGGGCTGTGC CACCCTCGGGTGAAAGTGGGCAATGAGTACGTCACCAAGGGGCAGAATGTCCAGCAGGTG ATATATGCCACTGGGGCACTGGCCAAGGCAGTGTATGAGAGGATGTTCAACTGGATGGTG ACGCGCATCAATGCCACCCTGGAGACCAAGCAGCCACGCCAGTACTTCATAGGAGTCCTG GACATCGCTGGCTTCGAGATCTTCGATTTCAACAGCTTTGAGCAGCTCTGCATCAACTTC ACCAACGAGAAGCTGCAGCAGTTCTTCAACCACCACATGTTTGTGCTGGAGCAGGAGGAG TACAAGAAGGAGGGCATCGAGTGGACATTCATTGACTTTGGCATGGACCTGCAGGCCTGC ATTGACCTCATCGAGAAGCCCATGGGCATCATGTCCATCCTGGAAGAGGAGTGCATGTTC CCCAAGGCCACCGACATGACCTTCAAGGCCAAGCTGTTTGACAACCACCTGGGCAAATCC GCCAACTTCCAGAAGCCACGCAATATCAAGGGGAAGCCTGAAGCCCACTTCTCCCTGATC CACTATGCCGGCATCGTGGACTACAACATCATTGGCTGGCTGCAGAAGAACAAGGATCCT CTCAATGAGACTGTCGTGGGCTTGTATCAGAAGTCTTCCCTCAAGCTGCTCAGCACCCTG TTTGCCAACTATGCTGGGGCTGATGCGCCTATTGAGAAGGGCAAAGGCAAGGCCAAGAAA GGCTCGTCCTTTCAGACTGTGTCAGCTCTGCACAGGGAAAATCTGAACAAGCTGATGACC AACTTGCGCTCCACCCATCCCCACTTTGTACGTTGTATCATCCCTAATGAGACAAAGTCT CCAGGGGTGATGGACAACCCCCTGGTCATGCACCAGCTGCGCTGCAATGGTGTGCTGGAG GGCATCCGCATCTGCAGGAAAGGCTTCCCCAACCGCATCCTCTACGGGGACTTCCGGCAG AGGTATCGCATCCTGAACCCAGCGGCCATCCCTGAGGGACAGTTCATTGATAGCAGGAAG GGGGCAGAGAAGCTGCTCAGCTCCCTGGACATTGATCACAACCAGTACAAGTTTGGCCAC ACCAAGGTGTTCTTCAAGGCCGGGCTGCTGGGGCTGCTGGAGGAAATGAGGGACGAGAGG CTGAGCCGCATCATCACGCGTATCCAGGCCCAGTCCCGAGGTGTGCTCGCCAGAATGGAG TACAAAAAGCTGCTGGAACGTAGAGACTCCCTGCTGGTAATCCAGTGGAACATTCGGGCC TTCATGGGGGTCAAGAATTGGCCCTGGATGAAGCTCTACTTCAAGATCAAGCCGCTGCTG AAGAGTGCAGAAAGAGAGAAGGAGATGGCCTCCATGAAGGAGGAGTTCACACGCCTCAAA GAGGCGCTAGAGAAGTCCGAGGCTCGCCGCAAGGAGCTGGAGGAGAAGATGGTGTCCCTG CTGCAGGAGAAGAATGACCTGCAGCTCCAAGTGCAGGCGGAACAAGACAACCTGGCAGAT GCTGAGGAGCGCTGTGATCAGCTGATCAAAAACAAGATTCAGCTGGAGGCCAAGGTGAAG GAGATGAACGAGAGGCTGGAGGATGAGGAGGAGATGAATGCTGAGCTCACTGCCAAGAAG CGCAAGCTGGAAGATGAGTGCTCAGAGCTCAAAAGGGACATCGATGATCTGGAGCTGACA CTGGCCAAAGTGGAGAAGGAGAAACACGCAACAGAGAACAAGGTGAAAAACCTGACAGAG GAGATGGCTGGGCTGGATGAGATCATCGCCAAGCTGACCAAGGAGAAGAAAGCTCTGCAA GAGGCCCACCAACAGGCTCTGGATGACCTTCAGGCCGAGGAGGACAAGGTCAACACCCTG ACTAAGGCCAAAGTCAAGCTGGAGCAGCAAGTGGATGATCTGGAAGGATCCCTGGAGCAA GAGAAGAAGGTGCGCATGGACCTGGAGCGAGCGAAGCGGAAGCTGGAGGGCGACCTGAAG CTGACCCAGGAGAGCATCATGGACCTGGAGAATGACAAGCAGCAGCTGGATGAGCGGCTG AAAAAAAAAGACTTTGAGCTGAATGCTCTCAACGCAAGGATTGAGGATGAACAGGCCCTC GGCAGCCAGCTGCAGAAGAAGCTCAAGGAGCTTCAGGCACGCATCGAGGAGCTGGAGGAG GAGCTGGAGGCCGAGCGCACCGCCAGGGCTAAGGTGGAGAAGCTGCGCTCAGACCTGTCT CGGGAGCTGGAGGAGATCAGCGAGCGGCTGGAAGAGGCCGGCGGGGCCACGTCCGTGCAG ATCGAGATGAACAAGAAGCGCGAGGCCGAGTTCCAGAAGATGCGGCGGGACCTGGAGGAG GCCACGCTGCAGCACGAGGCCACTGCCGCGGCCCTGCGCAAGAAGCACGCCGACAGCGTG GCCGAGCTGGGCGAGCAGATCGACAACCTGCAGCGGGTGAAGCAGAAGCTGGAGAAGGAG AAGAGCGAGTTCAAGCTGGAGCTGGATGACGTCACCTCCAACATGGAGCAGATCATCAAG GCCAAGGCTAACCTGGAGAAGATGTGCCGGACCTTGGAAGACCAGATGAATGAGCACCGG AGCAAGGCGGAGGAGACCCAGCGTTCTGTCAACGACCTCACCAGCCAGCGGGCCAAGTTG CAAACCGAGAATGGTGAGCTGTCCCGGCAGCTGGATGAGAAGGAGGCACTGATCTCCCAG CTGACCCGAGGCAAGCTCACCTACACCCAGCAGCTGGAGGACCTCAAGAGGCAGCTGGAG GAGGAGGTTAAGGCGAAGAACGCCCTGGCCCACGCACTGCAGTCGGCCCGGCATGACTGC GACCTGCTGCGGGAGCAGTACGAGGAGGAGACGGAGGCCAAGGCCGAGCTGCAGCGCGTC CTTTCCAAGGCCAACTCGGAGGTGGCCCAGTGGAGGACCAAGTATGAGACGGACGCCATT CAGCGGACTGAGGAGCTCGAGGAGGCCAAGAAGAAGCTGGCCCAGCGGCTGCAGGAAGCT GAGGAGGCCGTGGAGGCTGTTAATGCCAAGTGCTCCTCGCTGGAGAAGACCAAGCACCGG CTACAGAATGAGATCGAGGACTTGATGGTGGACGTAGAGCGCTCCAATGCTGCTGCTGCA GCCCTGGACAAGAAGCAGAGGAACTTCGACAAGATCCTGGCCGAGTGGAAGCAGAAGTAT GAGGAGTCGCAGTCGGAGCTGGAGTCCTCGCAGAAGGAGGCTCGCTCCCTCAGCACAGAG CTCTTCAAACTCAAGAACGCCTATGAGGAGTCCCTGGAACATCTGGAGACCTTCAAGCGG GAGAACAAAAACCTGCAGGAGGAGATCTCCGACTTGACTGAGCAGTTGGGTTCCAGCGGA AAGACTATCCATGAGCTGGAGAAGGTCCGAAAGCAGCTGGAGGCCGAGAAGATGGAGCTG CAGTCAGCCCTGGAGGAGGCCGAGGCCTCCCTGGAGCACGAGGAGGGCAAGATCCTCCGG GCCCAGCTGGAGTTCAACCAGATCAAGGCAGAGATCGAGCGGAAGCTGGCAGAGAAGGAC GAGGAGATGGAACAGGCCAAGCGCAACCACCTGCGGGTGGTGGACTCGCTGCAGACCTCC CTGGACGCAGAGACACGCAGCCGCAACGAGGCCCTGAGGGTGAAGAAGAAGATGGAAGGA GACCTCAATGAGATGGAGATCCAGCTCAGCCACGCCAACCGCATGGCCGCCGAGGCCCAG AAGCAAGTCAAGAGCCTCCAGAGCTTGTTGAAGGACACCCAGATTCAGCTGGACGATGCA GTCCGTGCCAACGACGACCTGAAGGAGAACATCGCCATCGTGGAGCGGCGCAACAACCTG CTGCAGGCTGAGCTGGAGGAGTTGCGTGCCGTGGTGGAGCAGACAGAGCGGTCCCGGAAG CTGGCGGAGCAGGAGCTGATTGAGACTAGTGAGCGGGTGCAGCTGCTGCATTCCCAGAAC ACCAGCCTCATCAACCAGAAGAAGAAGATGGATGCTGACCTGTCCCAGCTCCAGACTGAA GTGGAGGAGGCAGTGCAGGAGTGCAGGAATGCTGAGGAGAAGGCCAAGAAGGCCATCACG GATGCCGCCATGATGGCAGAGGAGCTGAAGAAGGAGCAGGACACCAGCGCCCACCTGGAG CGCATGAAGAAGAACATGGAACAGACCATTAAGGACCTGCAGCACCGGCTGGACGAAGCC GAGCAGATCGCCCTCAAGGGCGGCAAGAAGCAGCTGCAGAAGCTGGAAGCGCGGGTGCGG GAGCTGGAGAATGAGCTGGAGGCCGAGCAGAAGCGCAACGCAGAGTCGGTGAAGGGCATG AGGAAGAGCGAGCGGCGCATCAAGGAGCTCACCTACCAGACGGAGGAGGACAGGAAAAAC CTGCTGCGGCTGCAGGACCTGGTAGACAAGCTGCAGCTAAAGGTCAAGGCCTACAAGCGC CAGGCCGAGGAGGCGGAGGAGCAAGCCAACACCAACCTGTCCAAGTTCCGCAAGGTGCAG CACGAGCTGGATGAGGCAGAGGAGCGGGCGGACATCGCCGAGTCCCAGGTCAACAAGCTG CGGGCCAAGAGCCGTGACATTGGCACGAAGGGCTTGAATGAGGAGTAG Clone variation with respect to NM_000257.2 189 c=>t;732 c=>t;1062 c=>t;2967 t=>c >OriGene 5' read for NM_000257 unedited
ACTATAGGGCGGCCGCGAATTCGGCACGAGGTCCCCTGCAGGCCTTGGCCCCTTTCCTCA TCTGTAGACACACTTGAGTAGCCCAGGCACAGCCATGGGAGATTCGGAGATGGCAGTCTT TGGGGCTGCCGCCCCCTACCTGCGCAAGTCAGAGAAGGAGCGGCTAGAAGCGCAGACCAG GCCTTTTGACCTCAAGAAGGATGTCTTCGTGCCTGATGACAAACAGGAGTTTGTCAAGGC CAAGATCGTGTCTCGAGAGGGTGGCAAAGTCACTGCCGAGACTGAGTATGGCAAGACAGT GACCGTGAAGGAGGACCAGGTGATGCAGCAGAACCCACCCAAGTTCGACAAAATCGAGGA CATGGCCATGCTGACCTTCCTGCATGAGCCCGCGGTGCTCTACAACCTCAAGGATCGCTA CGGCTCCTGGATGATCTACACCTACTCGGGCCTCTTCTGTGTCACCGTCAACCCTTACAA GTGGCTGCCGGTGTACACTCCTGAGGTGGTGGCTGCCTACCGGGGCAAGAAGAGGAGCGA GGCCCCGCCCCACATCTTCTCCATCTCCGACAACGCCTATCAGTACATGCTGACAGACAG AGAANACCAGTCCATCCTGATCACCGGAGAATCCGGAGCAGGGAAGACAGTCAACACCAA GAGGGTCATCCAGTACTTTGCTGTTATTGCAGCCATTGGGGACCGCAGCAAGAAGGACCA GAGCCCGGGCAAGGGCAC >OriGene 3' read for NM_000257 unedited
CAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCACGAAACAAC TATGACCGCGGCCGCAATCTAAAGTCCAGTTTTTTTTTTTTTTTTTTTTTTCGGCTTCAA GGAAAAATGCTTTATTCTGCTTCCTCCCAAGGAACTGTTACACAAGCTCCAACATGGGGC TTTGCTGGCACCTCCAGGGCTGAACAAATCAAAAAGTGGCAAAACTACTCCTCATTCAAA CCCTTCGTGCCAATGTCACGGCTCTTGGCCCGCAACTTGGTGACCTGGGACTCGGCGATG TTCGCCCGCTTCTTTGGCTTAACCAACTTGTGCTGGACCTTGCGGAACTTGGACAAGTTG GTGTTGGCTTGCTCCTCCGCCTCCTTGGCCTGGCGCTTGTAAGCCTTGACCTTTAACTGC AGCTTGTCTACCAAGTCCTGCAACCGCAACAAGTTTTTCCTGTCCTCCTCCGTCTGGTAA GTGAGCTCCTTGATGCGCCGCTCGCTCTTCCTCATGCCCTTCACCGACTCTGCGTTGCGC TTCTGCTCGGCCTCCAGCTCATTCTCCAGCTCCCGCACCCGGGCTTCCAGCTTCTGCAGC TGCTTTCTTGCCGCCCTTTGAGGGCGAATCTTGCTCGGGCTTTCGTCCCAAGCCCGGTGG CTTGCAGGGTCCCTTAAATGGGGTCTGTTTCCAATGTTTCTTTCTTTCATGGCGCCTCCC AAGTTGGGGCCGCCTGGGTTGTTCCCTGGCTCCCTTTCTTTTAAACTTTCCTTCTG |
| Restriction Sites | Please inquire |
| ACCN | NM_000257 |
| Insert Size | 6000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000257.1, NP_000248.1 |
| RefSeq Size | 6008 bp |
| RefSeq ORF | 5808 bp |
| Locus ID | 4625 |
| UniProt ID | P12883 |
| Domains | IQ, myosin_head, Myosin_tail, M, Myosin_N |
| Protein Pathways | Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), Tight junction, Viral myocarditis |
| Gene Summary | Muscle myosin is a hexameric protein containing 2 heavy chain subunits, 2 alkali light chain subunits, and 2 regulatory light chain subunits. This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading. Mutations in this gene are associated with familial hypertrophic cardiomyopathy, myosin storage myopathy, dilated cardiomyopathy, and Laing early-onset distal myopathy. [provided by RefSeq, Jul 2008] |
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