ATG4A (NM_178271) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	APG4A; AUTL2
Vector	pCMV6-XL6
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC124770 sequence for NM_178271 edited (data generated by NextGen Sequencing) ATGCTACGCTGTGGACAGATGATGCTGGCTCAAGCCCTTATCTGTAGACACTTGGGAAGG GACTGGAGCTGGGAGAAACAAAAAGAACAACCCAAAGAATACCAACGCATCCTACAGTGC TTCTTAGATAGAAAAGATTGTTGCTACTCTATCCATCAAATGGCACAAATGGGTGTAGGA GAAGGGAAATCAATTGGAGAATGGTTTGGACCAAATACAGTTGCACAGGTGTTAAAAAAA CTTGCTTTATTTGACGAATGGAATTCCTTGGCTGTTTATGTTTCAATGGATAACACAGTG GTCATTGAAGATATCAAAAAAATGTGCCGTGTCCTTCCCTTGAGTGCTGACACAGCTGGT GACAGGCCTCCCGATTCTTTAACTGCTTCAAACCAGAGTAAGGGCACCTCTGCCTACTGC TCAGCCTGGAAACCCCTGCTGCTCATTGTGCCCCTTCGCCTGGGCATAAACCAAATCAAT CCTGTCTATGTTGATGCATTCAAAGAGTGTTTTAAGATGCCACAGTCTTTAGGGGCATTA GGAGGAAAACCAAATAACGCGTATTATTTCATAGGATTCTTAGGTGACGAGCTCATCTTC TTGGACCCTCATACAACCCAGACCTTTGTTGACACTGAAGAGAATGGAACGGTTAATGAC CAGACTTTCCATTGCCTGCAGTCCCCACAGCGAATGAACATCCTAAACCTGGATCCTTCA GTTGCATTGGGATTTTTCTGCAAAGAAGAAAAAGACTTTGATAACTGGTGTAGCCTTGTT CAGAAGGAAATTCTAAAGGAGAATTTAAGGATGTTTGAATTAGTTCAGAAACATCCATCA CACTGGCCTCCCTTTGTACCTCCAGCCAAGCCAGAAGTGACAACCACTGGGGCAGAATTC ATTGACTCTACTGAGCAACTGGAGGAGTTTGATCTGGAGGAAGATTTTGAGATTCTGAGT GTGTAG Clone variation with respect to NM_178271.1 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_178271 unedited ATATCCCCCGCCCGTTGCAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAG CAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGC GAATTCGGCACGAGGGTGGAATTGGCCCAGGATGACAGCTGGAGAATGGAGTCAGTTTTA TCCAAGTATGAAGATCAGATTACTATTTTCACTGACTACCTAGAAGAATATCCAGATACA GATGAGCTGGTATGGATCTTAGGGAAGCAGCATCTCCTTAAAACAGAAAAATCTAAGCTG TTGTCTGATATAAGTGCTCGTCTATGGTTTACATACAGAAGGAAATTTTCACCAATTGGT AGGTGGAACGGGCCCTTCATCAGATGCTGGTTGGGGATGTATGCTACGCTGTGGACAGAT GATGCTGGCTCAAGCCCTTATCTGTAGACACTTGGGAAGGGACTGGAGCTGGGAGAAACA AAAAGAACAACCCAAAGAATACCAACGCATCCTACAGTGCTTCTTAGATAGAAAAGAGTG TTGCTACTCTATCCATCAAATGGCACAAATGGGTGTAGGAGAAGGGAAATCAATTGGAGA ATGGTTTGGACCAAATACAGTTGCACAGGTGTTAAAAAAACTTGCTTTATTTGACGAATG GAATTCCTTGGCTGTTTATGTTTCAATGGATAACACAGTGGTCATTGAAGATATCAAAAA AATGTGGCTGTGTCCTTCCCATTGAGTGCTGACACAGGCTGGTGACAGGCCTCCCGATTC TTTCACTGCTTNCAACCAGAGTAAGGGCACCTCTGCCTACTGCTCAGNCTGGCAACCCCT GCTGCTCATTGTGCTCCTTCGCCTGGGCATACACCAAATCAATCTGTCTATGGTGATGCA TTCAAAGAGTGTTTTACGATGCCCAGTCTTTAGGGGCATTGGAGCACACCAATAC
Restriction Sites	NotI-NotI
ACCN	NM_178271
Insert Size	2500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_178271.1, NP_840055.1
RefSeq Size	2605 bp
RefSeq ORF	966 bp
Locus ID	115201
UniProt ID	Q8WYN0
Protein Families	Protease
Protein Pathways	Regulation of autophagy
Gene Summary	Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. [provided by RefSeq, Mar 2016]

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