GALE (BC001273) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FLJ95174; FLJ97302; galactose-4-epimerase, UDP-; galactowaldenase; OTTHUMP00000002991; OTTHUMP00000044857; SDR1E1; short chain dehydrogenase/reductase family 1E, member 1; UDP-galactose-4-epimerase; UDP galactose-4'-epimerase
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC001273 edited GGGAACCCGAGCAGGACTCTCCAGTCCTCAGTCACCTTGGACAAAGAAGTGTGGATCCTC AGATTCCATCTTTTCCAACTCCAAGGTGCCATGGCAGAGAAGGTGCTGGTAACAGGTGGG GCTGGCTACATTGGCAGCCACACGGTGCTGGAGCTGCTGGAGGCTGGCTACTTGCCTGTG GTCATCGATAACTTCCATAATGCCTTCCGTGGAGGGGGCTCCCTGCCTGAGAGCCTGCGG CGGGTCCAGGAGCTGACAGGCCGCTCTGTGGAGTTTGAGGAGATGGACATTTTGGACCAG GGAGCCCTACAGCGTCTCTTCAAAAAGTACAGCTTTATGGCGGTCATCCACTTTGCGGGG CTCAAGGCCGTGGGCGAGTCGGTGCAGAAGCCTCTGGATTATTACAGAGTTAACCTGACC GGGACCATCCAGCTTCTGGAGATCATGAAGGCCCACGGGGTGAAGAACCTGGTGTTCAGC AGCTCAGCCACTGTGTACGGGAACCCCCAGTACCTGCCCCTTGATGAGGCCCACCCCACG GGTGGTTGTACCAACCCTTACGGCAAGTCCAAGTTCTTCATCGAGGAAATGATCCGGGAC CTGTGCCAGGCAGACAAGACTTGGAACGCAGTGCTGCTGCGCTATTTCAACCCCACAGGT GCCCATGCCTCTGGCTGCATTGGTGAGGATCCCCAGGGCATACCCAACAACCTCATGCCT TATGTCTCCCAGGTGGCGATCGGGCGACGGGAGGCCCTGAATGTCTTTGGCAATGACTAT GACACAGAGGATGGCACAGGTGTCCGGGATTACATCCATGTCGTGGATCTGGCCAAGGGC CACATTGCAGCCTTAAGGAAGCTGAAAGAACAGTGTGGCTGCCGGATCTACAACCTGGGC ACGGGCACAGGCTATTCAGTGCTGCAGATGGTCCAGGCTATGGAGAAGGCCTCTGGGAAG AAGATCCCGTACAAGGTGGTGGCACGGCGGGAAGGTGATGTGGCAGCCTGTTACGCCAAC CCCAGCCTGGCCCAAGAGGAGCTGGGGTGGACAGCAGCCTTAGGGCTGGACAGGATGTGT GAGGATCTCTGGCGCTGGCAGAAGCAGAATCCTTCAGGCTTTGGCACGCAAGCCTGAGGA CCCTCCCCTACCAAGGACCAGGAAAAGCAGCAGCTGCCTGCTCTCCAGCCTCTGGCAGGA ACTCAGGGCCCTGGAGCTGCTGGGGCCAAGCCAAGGCCTCCCCTACCTCAAACCCCAGCT GGGCCCGCTTAGCCCACCAGGCATGAGGCCAAGGGCTCCACTGACCAGGAGGCCGAGGTC TCTAACTCTTATCTTCCACAGGGTCCAAGAGTTCATCAGGACCCCCAAGAGTGAGTGAGG GGGCAAGGCTCTGGCACAAAACCTCCTCCTCCCAGGCACTCATTTATATTGCTCTGAAAG AGCTTTCCAAAGTATTTAAAAATAAAAACAAGTTTTCTTACACTGGGAAAAAAAAAAAAA AAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC001273 unedited AAACTGATTTGTAAACCATTATATAGGCGGCCGCGAATCCCGGGNAATCGTCGACCCACG CGTCCGGGGAACCCGAGCAGGACTCTCCAGTCCTCAGTCACCTTGGACAAAGAAGTGTGG ATCCTCAGATTCCATCTTTTCCAACTCCAAGGTGCCATGGCAGAGAAGGTGCTGGTAACA GGTGGGGCTGGCTACATTGGCAGCCACACGGTGCTGGAGCTGCTGGAGGCTGGCTACTTG CCTGTGGTCATCGATAACTTCCATAATGCCTTCCGTGGAGGGGGCTCCCTGCCTGAGAGC CTGCGGCGGGTCCAGGAGCTGACAGGCCGCTCTGTGGAGTTTGAGGAGATGGACATTTTG GACCAGGGAGCCCTACAGCGTCTCTTCAAAAAGTACAGCTTTATGGCGGTCATCCACTTT GCGGGGCTCAAGGCCGTGGGCGAGTCGGTGCAGAAGCCTCTGGATTATTACAGAGTTAAC CTGACCGGGACCATCCAGCTTCTGGAGATCATGAAGGCCCACGGGGTGAAGAACCTGGTG TTCAGCAGCTCAGCCACTGTGTACGGGAACCCCCAGTACCTGCCCCTTGATGAGGCCCAC CCCACGGGTGGTTGTACCAACCCTTACGGCAAGTCCAAGTTCTTCATCGAGGAAATGATC CGGGACCTGTGCCAGGCAGACAAGACTTGGAACGCAGTGCTGCTGCGCTATTTCAACCCC ACAGGTGCCCATGCCTCTGGCTGCATTGGTGAGGATCCCCAGGGCATACCCAACAACCTC ATGCCTTATGTCTCCCCAGTGGCGATCGGGCGACGGGAGGCCCTGAATGTCTTTTGCATG ACTATGACACAGAGGATGGCACAGGTGTCCGGGATTACATCCATGTCGTGGATCTTGCCC AGGGCACATTGCAGCCTTAG
Restriction Sites	Please inquire
ACCN	BC001273
Insert Size	1503 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC001273.1, AAH01273.1
RefSeq Size	1503 bp
Locus ID	2582
Protein Families	Druggable Genome
Protein Pathways	Amino sugar and nucleotide sugar metabolism, Galactose metabolism, Metabolic pathways
Gene Summary	This gene encodes UDP-galactose-4-epimerase which catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids. Mutations in this gene result in epimerase-deficiency galactosemia, also referred to as galactosemia type 3, a disease characterized by liver damage, early-onset cataracts, deafness and cognitive disability, with symptoms ranging from mild ('peripheral' form) to severe ('generalized' form). Multiple alternatively spliced transcripts encoding the same protein have been identified. [provided by RefSeq, Jul 2008]

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