ALG11 (BC010857) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	asparagine-linked glycosylation 11 homolog (S. cerevisiae, alpha-1,2-mannosyltransferase); asparagine-linked glycosylation 11 homolog (yeast, alpha-1,2-mann; GT8; KIAA0266; OTTHUMP00000018446; UTP14, U3 small nucleolar ribonucleoprotein, homolog C; UTP14C
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC010857 edited CCCACGCGTCCGGTTTGTTTTTTTAAGGAAACGCTATCTTGTGGAAGATTCACTGTATCC TCACTTCACACTGCTGGGCCAAAGTCTAGGATCCATTTTTCTTGGCTGGGAAGCTCTAAT GCAGTGTGTTCCTGATGTTTACATTGATTCAATGGGATACGCTTTTACGCTTCCTCTGTT TAAGTATATAGGGGGTTGCCAAGTTGGAAGCTATGTTCATTATCCTACTATCAGCACCGA CATGCTCTCTGTAGTGAAGAATCAAAATATTGGATTTAATAATGCAGCCTTCATTACCAG GAATCCTTTTCTCAGCAAAGTAAAGCTCATCTACTACTATTTATTTGCTTTTATTTATGG ACTTGTTGGTTCTTGCAGTGATGTAGTCATGGTCAATTCTTCTTGGACACTAAACCATAT TCTCTCACTATGGAAAGTTGGGAATTGCACTAACATTGTTTATCCACCTTGTGATGTGCA GACATTTCTGGACATTCCCTTACATGAGAAAAAGATGACCCCAGGACATTTGCTGGTTTC TGTTGGCCAGTTTAGGCCGGAAAAGAATCATCCATTGCAGATCAGAGCCTTTGCTAAATT GCTGAATAAGAAGATGGTTGAGTCACCTCCTTCGCTTAAACTTGTCCTCATTGGAGGTTG TCGTAACAAAGATGATGAACTTAGGGTAAACCAACTGAGAAGGCTGTCTGAGGATTTAGG AGTTCAAGAATATGTGGAATTTAAAATAAACATTCCATTTGATGAATTAAAGAATTATTT GTCTGAAGCAACAATTGGTCTGCATACCATGTGGAACGAGCATTTTGGGATTGGAGTTGT GGAGTGTATGGCAGCTGGCACAATTATCCTTGCACACAATTCGGGGGGCCCAAAGCTTGA CATTGTGGTTCCTCACGAAGGAGATATAACTGGCTTTCTGGCTGAGAGTGAAGAAGACTA TGCTGAAACTATCGCTCACATTCTTTCCATGTCTGCAGAAAAGAGACTCCAAATCAGAAA AAGTGCTCGTGCATCTGTAAGCAGATTCTCTGATCAGGAATTTGAAGTGACATTCCTATC ATCTGTGGAAAAGTTATTTAAGTAATGCCATATCTGTAAAATTAAAGATATTTTATATAA ACTGGTTAAACACCTTCATATGTAAATATTTTTCTAAATTCAATCTCATTTGTCAAATCA TTTTACTTTAGAAAACAGACAAAATTTCCTTTTAGAATAAAAGGAAGTGTTGAAAAGAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC010857 unedited NNNGGTTGGGAGNNGNAAACGACTTATATAGGCGGGCCGCGATTCCCGGGATATCGTCGA CCCACGCGTCCGCCCACGCGTCCGGTTTGTTTTTTTAAGGAAACGCTATCTTGTGGAAGA TTCACTGTATCCTCACTTCACACTGCTGGGCCAAAGTCTAGGATCCATTTTTCTTGGCTG GGAAGCTCTAATGCAGTGTGTTCCTGATGTTTACATTGATTCAATGGGATACGCTTTTAC GCTTCCTCTGTTTAAGTATATAGGGGGTTGCCAAGTTGGAAGCTATGTTCATTATCCTAC TATCAGCACCGACATGCTCTCTGTAGTGAAGAATCAAAATATTGGATTTAATAATGCAGC CTTCATTACCAGGAATCCTTTTCTCAGCAAAGTAAAGCTCATCTACTACTATTTATTTGC TTTTATTTATGGACTTGTTGGTTCTTGCAGTGATGTAGTCATGGTCAATTCTTCTTGGAC ACTAAACCATATTCTCTCACTATGGAAAGTTGGGAATTGCACTAACATTGTTTATCCACC TTGTGATGTGCAGACATTTCTGGACATTCCCTTACATGAGAAAAAGATGACCCCAGGACA TTTGCTGGTTTCTGTTGGCCAGTTTAGGCCGGANAAGAATCATCCATTGCAGATCAGAGC CTTTGCTAAATTGCTGAATAAGAAGATGGTTGAGTCACCTCCTTCGCTTAAACTTGTCCT CATTGGAGGTTGTCGTACCAAGATGATGAACTTANGGTAAACCAACTGAGAAGGCTGTCT GANGATTTANGAGTTCAAGATATGTGGAATTTAAATAACATTCCATTTGATGATTAAAGA ATATNTGTCTGAAGCAACATTGGTCTGCATACCATGTGGAACGAGCATTTTGGATT
Restriction Sites	Please inquire
ACCN	BC010857
Insert Size	1316 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC010857.1, AAH10857.1
RefSeq Size	1316 bp
Locus ID	440138
Protein Families	Transmembrane
Protein Pathways	Metabolic pathways, N-Glycan biosynthesis
Gene Summary	This gene encodes a GDP-Man:Man3GlcNAc2-PP-dolichol-alpha1,2-mannosyltransferase which is localized to the cytosolic side of the endoplasmic reticulum (ER) and catalyzes the transfer of the fourth and fifth mannose residue from GDP-mannose (GDP-Man) to Man3GlcNAc2-PP-dolichol and Man4GlcNAc2-PP-dolichol resulting in the production of Man5GlcNAc2-PP-dolichol. Mutations in this gene are associated with congenital disorder of glycosylation type Ip (CDGIP). This gene overlaps but is distinct from the UTP14, U3 small nucleolar ribonucleoprotein, homolog C (yeast) gene. A pseudogene of the GDP-Man:Man3GlcNAc2-PP-dolichol-alpha1,2-mannosyltransferase has been identified on chromosome 19. [provided by RefSeq, Aug 2010]

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