ULK3 (NM_015518) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FLJ90566, DKFZP434C131
Vector	pCMV6-XL6
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_015518, the custom clone sequence may differ by one or more nucleotides ATGGCGGGGCCCGGCTGGGGTCCCCCGCGCCTGGACGGCTTCATCCTCACCGAGCGCCTGGGCAGCGGCA CGTACGCCACGGTGTACAAGGCCTACGCCAAGAAGGACACTCGTGAAGTGGTAGCCATAAAGTGTGTAGC CAAGAAAAGTCTGAACAAGGCATCGGTGGAGAACCTCCTCACGGAGATTGAGATCCTCAAGGGCATTCGA CATCCCCACATTGTGCAGCTGAAAGACTTTCAGTGGGACAGTGACAATATCTACCTCATCATGGAGTTTT GCGCAGGGGGCGACCTGTCTCGCTTCATCCATACCCGCAGGATTCTGCCTGAGAAGGTGGCGCGTGTCTT CATGCAGCAATTAGCTAGCGCCCTGCAATTCCTGCATGAACGGAATATCTCTCACCTGGATCTGAAGCCA CAGAACATTCTACTGAGCTCCTTGGAGAAGCCCCACCTAAAACTGGCAGACTTTGGTTTCGCACAACACA TGTCCCCGTGGGATGAGAAGCACGTGCTCCGTGGCTCCCCCCTCTACATGGCCCCCGAGATGGTGTGCCA GCGGCAGTATGACGCCCGCGTGGACCTCTGGTCCATGGGGGTCATCCTGTATGGTGAGACCTCTTTTCCC TGCTTCTCACCCTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_015518 unedited GGGGGNNTGGGGTNNATNATATATATTTTTATNNNGGTTTTTACACCCGCCCGTTGNCGC AAAGGGCGGTAGGCGTGTACGGTGGNGAGTCTATATAAGCAGAGCTCATTTAGGTGACAC TATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGGCCGCC CGCAGCGAGGAAGCGCCCGCGCGGGCGCAGGCGGCCGGAATGGCGGGGCCCGGCTGGGGT CCCCCGCGCCTGGACGGCTTCATCCTCACCGAGCGCCTGGGCAGCGGCACGTACGCCACG GTGTACAAGGCCTACGCCAAGAAGGACACTCGTGAAGTGGTAGCCATAAAGTGTGTAGCC AAGAAAAGTCTGAACAAGGCATCGGTGGAGAACCTCCTCACGGAGATTGAGATCCTCAAG GGCATTCGACATCCCCACATTGTGCAGCTGAAAGACTTTCAGGTGTGAGCCTGGGGCGGG GCCGCTGCCAAAGGGAGCTGGAGAAGGGACACTCTCTTCAGGCCGCCTCTCTGCCTCTAA ACACAGTGGGACAGTGACAATATCTACCTCATCATGGAGTTTTGCGCAGGGGGCGACCTG TCTCGCTTCATCCATACCCGCAGGATTCTGCCTGAGAAGGTGGCGCGTGTCTTCATGCAG CAATTAGCTAGCGCCCTGCAATTCCTGCATGAACGGAATATCTCTCACCTGGATCTGAAG CCACAGAACATTCTACTGAGCTCCTTGGAGAAGCCCCACCTAAAACTGGCAGACTNTGGT TTCGCACAACACATGTCCCCGTGGGATGAGAAGCACGTGCTCCGTGGCTCCCCCCTCTAC ATGGNCCCCCGAGAGGTGTGCCAGCGGCAGTATGACGCCCGCGTGGACCTCTGGTCCATG GGGGTCATCCTGTATGAAGCCCTCTTCGGCAGCCCCCCTAG
Restriction Sites	NotI-NotI
ACCN	NM_015518
Insert Size	5000 bp
OTI Disclaimer	The sequence of an 'OriGene Unique Variant' differs significantly from the associated reference. It represents a novel splice variant from the same gene locus of the reference. Although such variants are true transcripts and present opportunity for discoveries, they are not yet curated by NCBI and should not be used if the exact reference accession sequence is required.
OTI Annotation	This TrueClone was found to represent an alternative form of the specific reference to which it is associated. Its Open Reading Frame (ORF) may represent a novel form or alternative splice variant. By virtue of it being a true transcript (cDNA clone not PCR product), it provides a biologically relevant copy of its mRNA template. For more details, please evaluate the sequence information provided on this website or contact our customer care specialists.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_015518.1, NP_056333.1
RefSeq Size	2941 bp
RefSeq ORF	645 bp
Locus ID	25989
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	mTOR signaling pathway, Regulation of autophagy
Gene Summary	Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.