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LCAT (NM_000229) Human Untagged Clone
CAT＃: SC120019
LCAT (untagged)-Human lecithin-cholesterol acyltransferase (LCAT)
CNY 2,950.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC120019 sequence for NM_000229 edited (data generated by NextGen Sequencing)
ATGGGGCCGCCCGGCTCCCCATGGCAGTGGGTGACGCTGCTGCTGGGGCTGCTGCTCCCT CCTGCCGCCCCCTTCTGGCTCCTCAATGTGCTCTTCCCCCCGCACACCACGCCCAAGGCT GAGCTCAGTAACCACACACGGCCCGTCATCCTCGTGCCCGGCTGCCTGGGGAATCAGCTA GAAGCCAAGCTGGACAAACCAGATGTGGTGAACTGGATGTGCTACCGCAAGACAGAGGAC TTCTTCACCATCTGGCTGGATCTCAACATGTTCCTACCCCTTGGGGTAGACTGCTGGATC GATAACACCAGGGTTGTCTACAACCGGAGCTCTGGGCTCGTGTCCAACGCCCCTGGTGTC CAGATCCGCGTCCCTGGCTTTGGCAAGACCTACTCTGTGGAGTACCTGGACAGCAGCAAG CTGGCAGGGTACCTGCACACACTGGTGCAGAACCTGGTCAACAATGGCTACGTGCGGGAC GAGACTGTGCGCGCCGCCCCCTATGACTGGCGGCTGGAGCCCGGCCAGCAGGAGGAGTAC TACCGCAAGCTCGCAGGGCTGGTGGAGGAGATGCACGCTGCCTATGGGAAGCCTGTCTTC CTCATTGGCCACAGCCTCGGCTGTCTACACTTGCTCTATTTCCTGCTGCGCCAGCCCCAG GCCTGGAAGGACCGCTTTATTGATGGCTTCATCTCTCTTGGGGCTCCCTGGGGTGGCTCC ATCAAGCCCATGCTGGTCTTGGCCTCAGGTGACAACCAGGGCATCCCCATCATGTCCAGC ATCAAGCTGAAAGAGGAGCAGCGCATAACCACCACCTCCCCCTGGATGTTTCCCTCTCGC ATGGCGTGGCCTGAGGACCACGTGTTCATTTCCACACCCAGCTTCAACTACACAGGCCGT GACTTCCAACGCTTCTTTGCAGACCTGCACTTTGAGGAAGGCTGGTACATGTGGCTGCAG TCACGTGACCTCCTGGCAGGACTCCCAGCACCTGGTGTGGAAGTATACTGTCTTTACGGC GTGGGCCTGCCCACGCCCCGCACCTACATCTACGACCACGGCTTCCCCTACACGGACCCT GTGGGTGTGCTCTATGAGGATGGTGATGACACGGTGGCGACCCGCAGCACCGAGCTCTGT GGCCTGTGGCAGGGCCGCCAGCCACAGCCTGTGCACCTGCTGCCCCTGCACGGGATACAG CATCTCAACATGGTCTTCAGCAACCTGACCCTGGAGCACATCAATGCCATCCTGCTGGGT GCCTACCGCCAGGGTCCCCCTGCATCCCCGACTGCCAGCCCAGAGCCCCCGCCTCCTGAA TAA Clone variation with respect to NM_000229.1 >OriGene 5' read for NM_000229 unedited
TATCCCCCGCCCCTTGCCGCATAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGC AGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGA ATTCGGCACCAGGGAATGGGGCCGCCCGGCTCCCCATGGCAGTGGGTGACGCTGCTGCTG GGGCTGCTGCTCCCTCCTGCCGCCCCCTTCTGGCTCCTCAATGTGCTCTTCCCCCCGCAC ACCACGCCCAAGGCTGAGCTCAGTAACCACACACGGCCCGTCATCCTCGTGCCCGGCTGC CTGGGGAATCAGCTAGAAGCCAAGCTGGACAAACCAGATGTGGTGAACTGGATGTGCTAC CGCAAGACAGAGGACTTCTTCACCATCTGGCTGGATCTCAACATGTTCCTACCCCTTGGG GTAGACTGCTGGATCGATAACACCAGGGTTGTCTACAACCGGAGCTCTGGGCTCGTGTCC AACGCCCCTGGTGTCCAGATCCGCGTCCCTGGCTTTGGCAAGACCTACTCTGTGGAGTAC CTGGACAGCAGCAAGCTGGCAGGGTACCTGCACACACTGGTGCAGAACCTGGTCAACAAT GGCTACGTGCGGGACGAGACTGTGCGCGCCGCCCCCTATGACTGGCGGCTGGAGCCCGGC CAGCAGGAGGAGTACTACCGCAAGCTCGCAGGGCTGGTGGAGGAGATGCACGCTGCCTAT GGGAAGCCTGTNCTCCTCATTGGCCACAGCCTCGGCTGTCTACACTTGCTCTATTTCCTG CTGCGCCAGCCCCAGGCCTGGAAGGACCGCTTTATTGATGGCTTCATCTCTCTTGGGGCT CCCTGGGGTGGCTNCATCAAGCCCATGCTGGTCTTGGCCTCAGTGACAACCAGGCATCCC ATCATGTCCAGCATCAAGCTGAG >OriGene 3' read for NM_000229 unedited
NNNAATTAGCTTGNNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTAC GGTAGCAAAGGAAGGTCTTTATTCAGGAGGCGGGGGCTCTGGGCTGGCAGTCGGGGATGC AGGGGGACCCTGGCGGTAGGCACCCAGCAGGATGGCATTGATGTGCTCCAGGGTCAGGTT GCTGAAGACCATGTTGAGATGCTGTATCCCGTGCAGGGGCAGCAGGTGCACAGGCTGTGG CTGGCGGCCCTGCCACAGGCCACAGAGCTCGGTGCTGCGGGTCGCCACCGTGTCATCACC ATCCTCATAGAGCACACCCACAGGGTCCGTGTAGGGGAAGCCGTGGTCGTAGATGTAGGT GCGGGGGCGTGGNCAGGCCCACGCCGTAAAGACAGTATACTTCCACACCAGGTGCTGGGA GTCCTGCCAGGAGGTCACGTGACTGCAGCCACATGTACCAGCCTTCCTCAAAGTGCAGGT CTGCAAAGAAGCGTTGGAAGTCACGGCCTGTGTAGTTGAAGCTGGGTGTGGAAATGAACA CGTGGTCCTCAAGCCACGCCATGCGAGAGGGAAACATCCAGGGGGAGGTGGTGGTTATGC GCTGCTCCTCTTTCAGCTTGATGCTGGACATGATGGGGATGCCCTGGTTGTCACCTGAAG CCAAGACCAGCATGGGCTTGATGGACCCACCCCAGGGAGCCCCAAGAGAGATGAAGCCAT CAATAAAGCGGTCCTTCAGGCCTGGGGCTGGCGCAACAGGAAATAGAGCAAGTGTAGACA GCCCGAGGCTGTGGCCCCATGAAGAAGAAAAGGCTTCCCATAGACAGCGTGCAATTTTCC TTCAACCACCCTGCGAGCTTTGGGGAAGTACTCCCTTCTGCTGGCCCGGGCTCAACCCGC CAGTCCTAAGGAGGCG |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_000229 |
| Insert Size | 1400 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000229.1, NP_000220.1 |
| RefSeq Size | 1354 bp |
| RefSeq ORF | 1323 bp |
| Locus ID | 3931 |
| UniProt ID | P04180 |
| Domains | LACT |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Glycerophospholipid metabolism |
| Gene Summary | This gene encodes the extracellular cholesterol esterifying enzyme, lecithin-cholesterol acyltransferase. The esterification of cholesterol is required for cholesterol transport. Mutations in this gene have been found to cause fish-eye disease as well as LCAT deficiency. [provided by RefSeq, Jul 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Identification and functional analysis of missense mutations in the lecithin cholesterol acyltransferase gene in a Chilean patient with hypoalphalipoproteinemia
,Tobar, HE;Cataldo, LR;González, T;Rodríguez, R;Serrano, V;Arteaga, A;Álvarez-Mercado, A;Lagos, CF;Vicuña, L;Miranda, JP;Pereira, A;Bravo, C;Aguilera, CM;Eyheramendy, S;Uauy, R;Martínez, Á;Gil, Á;Francone, O;Rigotti, A;Santos, JL;,
Lipids Health Dis
,PubMed ID 31164121
[LCAT]
|
|
Effect of Recombinant Human Lecithin Cholesterol Acyltransferase Infusion on Lipoprotein Metabolism in Mice
,Xavier Rousset, Boris Vaisman, Bruce Auerbach, Brian R. Krause, Reyn Homan, John Stonik, Gyorgy Csako, Robert Shamburek, and Alan T. Remaley,
J. Pharmacol. Exp. Ther., Oct 2010; 335: 140 - 148
[LCAT]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203763 | LCAT (Myc-DDK-tagged)-Human lecithin-cholesterol acyltransferase (LCAT) |
CNY 3,656.00 |
|
| RC203763L1 | Lenti ORF clone of Human lecithin-cholesterol acyltransferase (LCAT), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203763L2 | Lenti ORF clone of Human lecithin-cholesterol acyltransferase (LCAT), mGFP tagged |
CNY 5,890.00 |
|
| RC203763L3 | Lenti ORF clone of Human lecithin-cholesterol acyltransferase (LCAT), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203763L4 | Lenti ORF clone of Human lecithin-cholesterol acyltransferase (LCAT), mGFP tagged |
CNY 5,890.00 |
|
| RG203763 | LCAT (tGFP-tagged) - Human lecithin-cholesterol acyltransferase (LCAT) |
CNY 4,370.00 |
|
| SC323736 | LCAT (untagged)-Human lecithin-cholesterol acyltransferase (LCAT) |
CNY 2,950.00 |
