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PLOD1 (NM_000302) Human Untagged Clone
CAT＃: SC119956
PLOD1 (untagged)-Human procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1 (PLOD1)
CNY 5,336.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | EDS6; EDSKCL1; LH; LH1; LLH; PLOD |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_000302 edited
ATGCGGCCCCTGCTGCTACTGGCCCTGCTGGGCTGGCTGCTGCTGGCCGAAGCGAAGGGC GACGCCAAGCCGGAGGACAACCTTTTAGTCCTCACGGTGGCCACTAAGGAGACCGAGGGA TTCCGTCGCTTCAAGCGCTCAGCTCAGTTCTTCAACTACAAGATCCAGGCGCTTGGCCTA GGGGAGGACTGGAATGTGGAGAAGGGGACGTCGGCAGGTGGAGGGCAGAAGGTCCGGCTG CTGAAGAAAGCTCTGGAGAAGCACGCAGACAAGGAGGATCTGGTCATTCTCTTCGCAGAC AGCTATGACGTGCTGTTTGCATCGGGGCCCCGGGAGCTCCTGAAGAAGTTCCGGCAGGCC AGGAGCCAGGTGGTCTTCTCTGCTGAGGAGCTCATCTACCCAGACCGCAGGCTGGAGACC AAGTATCCGGTGGTGTCCGATGGCAAGAGGTTCCTGGGCTCTGGAGGCTTCATCGGTTAT GCCCCCAACCTCAGCAAACTGGTGGCCGAGTGGGAGGGCCAGGACAGCGACAGCGATCAG CTGTTTTACACCAAGATCTTCTTGGACCCGGAGAAGAGGGAGCAGATCAATATCACCCTG GACCACCGCTGCCGTATCTTCCAGAACCTGGATGGAGCCTTGGATGAGGTCGTGCTCAAG TTTGAAATGGGCCATGTGAGAGCGAGGAACCTGGCCTATGACACCCTCCCGGTCCTGATC CATGGCAACGGGCCAACCAAGCTGCAGTTGAACTACCTGGGCAACTACATCCCGCGCTTC TGGACCTTCGAAACAGGCTGCACCGTGTGTGACGAAGGCTTGCGCAGCCTCAAGGGCATT GGGGATGAAGCTCTGCCCACGGTCCTGGTCGGCGTGTTCATCGAACAGCCCACGCCGTTT GTGTCCCTGTTCTTCCAGCGGCTCCTGCGGCTCCACTACCCCCAGAAACACATGCGACTT TTCATCCACAACCACGAGCAGCACCACAAGGCTCAGGTGGAAGAGTTCCTGGCACAGCAT GGCAGCGAGTACCAGTCTGTGAAGCTGGTGGGCCCTGAGGTGCGGATGGCGAATGCAGAT GCCAGGAACATGGGCGCAGACCTGTGCCGGCAGGACCGCAGCTGCACCTACTACTTCAGC GTGGATGCTGACGTGGCCCTGACCGAGCCCAACAGCCTGCGGCTGCTGATCCAACAGAAC AAGAACGTCATTGCCCCGCTGATGACCCGGCATGGGAGGCTGTGGTCGAACTTCTGGGGG GCTCTCAGTGCAGATGGCTACTATGCCCGTTCCGAGGACTACGTGGACATTGTGCAGGGG CGGCGTGTTGGTGTCTGGAATGTGCCCTATATTTCAAACATCTACTTGATCAAGGGCAGT GCCCTGCGGGGTGAGCTGCAGTCCTCAGATCTCTTCCACCACAGCAAGCTGGACCCCGAC ATGGCCTTCTGTGCCAACATCCGGCAGCAGGATGTGTTCATGTTCCTGACCAACCGGCAC ACCCTTGGCCATCTGCTCTCCCTAGACAGCTACCGCACCACCCACCTGCACAACGACCTC TGGGAGGTGTTCAGCAACCCCGAGGACTGGAAGGAGAAGTACATCCACCAGAACTACACC AAAGCCCTGGCAGGGAAGCTGGTGGAGACGCCCTGCCCGGATGTCTATTGGTTCCCCATC TTCACGGAGGTGGCCTGTGATGAGCTGGTGGAGGAGATGGAGCACTTTGGCCAGTGGTCT CTGGGCAACAACAAGGACAACCGCATCCAGGGTGGCTACGAGAACGTGCCGACTATTGAC ATCCACATGAACCAGATCGGCTTTGAGCGGGAGTGGCACAAATTCCTGCTGGAGTACATT GCGCCCATGACGGAGAAGCTCTACCCCGGCTACTACACCAGGGCCCAGTTTGACCTGGCC TTTGTCGTCCGCTACAAGCCTGATGAGCAGCCCTCACTGATGCCACACCATGATGCCTCC ACCTTCACCATCAACATCGCCCTGAACCGAGTCGGGGTGGATTACGAGGGCGGGGGCTGT CGGTTCCTGCGCTACAACTGTTCCATCCGAGCCCCAAGGAAGGGCTGGACCCTCATGCAC CCTGGACGACTCACGCATTACCATGAGGGGCTCCCCACCACCAGGGGCACCCGCTACATC GCAGTCTCCTTCGTCGATCCCTAA >OriGene 5' read for NM_000302 unedited
NGGTGTTCAAAATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCGGGAC CTGCGGCCCCGNTCGCGAGTTTCCAGCCCTGCGAGCGCCGCCGGGTCGGCCGATCGTCCC CCATACCTCGGCCATGCGGCCCCTGCTGCTACTGGCCCTGCTGGGCTGGCTGCTGCTGGC CGAAGCGAAGGGCGACGCCAAGCCGGAGGACAACCTTTTAGTCCTCACGGTGGCCACTAA GGAGACCGAGGGATTCCGTCGCTTCAAGCGCTCAGCTCAGTTCTTCAACTACAAGATCCA GGCGCTTGGCCTAGGGGAGGACTGGAATGTGGAGAAGGGGACGTCGGCAGGTGGAGGGCA GAAGGTCCGGCTGCTGAAGAAAGCTCTGGAGAAGCACGCAGACAAGGAGGATCTGGTCAT TCTCTTCGCAGACAGCTATGACGTGCTGTTTGCATCGGGGCCCCGGGAGCTCCTGAAGAA GTTCCGGCAGGCCAGGAGCCAGGTGGTCTTCTCTGCTGAGGAGCTCATCTACCCAGACCG CAGGCTGGAGACCAAGTATCCGGTGGTGTCCGATGGCAAGAGGTTCCTGGGCTCTGGAGG CTTCATCGGTTATGCCCCCAACCTCAGCAAACTGGTGGCCGAGTGGGAGGGCCAGGACAG CGACAGCGATCAGCTGTTTTACACCAAGATCTTCTTGGACCCGGAGAAGAAGGAGCAGAT CAATATCACCCTGGACCACCGCTGCCGTATCTTCCAGACCTGGATGGAGCCTTGGATGAA GTCGTGCTCAAGTTTGAAATGGCCATGTGAGAGCGAAGAACCTGGCCTATGACACCCTCC CCGTCCTGATCCATGGCAAACGGGCCACCAAGCTGCAGTTGAACTACCTGGNGCACTACA TNCCG >OriGene 3' read for NM_000302 unedited
CTTTATAGAACTCTCCGTTTCTGACGGTCTGTCCCTCTCTTCANAGCATCCAGGACAGGG TGACCCAGTGTGGTGTGTGGACCTGTCACTCCCCTCCCCACAGGCAAGTAGAAGATCGAG CTGTGCACAGATGCCTCTTCTGTCTCCCAAGAGGTTCTCTGGAGTCGCTGTCTCCAGGGG ACTACGGTTTGCTCTCATGACCAGCCATGGAGGCAGAAGTCCCAGACTCAGGGCAGCCCT TGGAGTCTGATCCAGGTGTCTTTACCCCAAAACTTGAAGCAGAAGTCCCTGGTGCAACAC CTGTGCCTGGGAGAAGCTCTGGCCATGGCTTCTCACCCAGACAGTCTTTCTGGGCAACTT GGGGAAGCCCCTGTTCTGCTCAAGTCTCACCCCATGGAAGAGGTGGGGGAAGGGGGCCTT GGTTTTTCAGGAAGACAGGTTGGAGAGCACGAGTCACTACAAAGCAGTAAAAGTGAATGG TGTCTCCAGGGGCTGGGTCCAGAACACCACGGAGAGCCCCAGCCATAAAGGTGTGTTCCG CCTCTGGCCTGCAGGAATCTCTTTGAATCTCTNTGATTGGTGGCTCCAAGAGCAATGGGA AGTCAACAGCCCAGAGGCTGGACTGNGTTCCCTGGGGACCCCGAGTCCCANNAGCTGCTG GGCAGTGGTTGTCGGCAAAGAAAGAAGGTCCCAGAGGGTCANGCCTGCCCAATANGGATC GACGAAGGAGACTGCGATGTACCGGGTGCCCCTGTTGGTGGGGAGCCCCTCATGGTATGC GTGAGTCGTCCAGGGTGCATGAGGGTTCACCCTTNCCTTGGGGCTCGAATGGACCAGTTG TACGCANGACCCGACAGNCCCCGCCTCGTNATCCACCCGACTGCGTCAGGGCCATTGTGA TGGTGAAGGCTGAGGCCTCATGGTGTGGCACTCATGAAGCCTGCTATCAAGTCTGATACG AACACAAGGCAGGTCAACTGGGCCCTGTGTATTACCGGGTAAAGCTTCCCTCATGGCCAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000302 |
| Insert Size | 3180 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000302.2, NP_000293.2 |
| RefSeq Size | 3004 bp |
| RefSeq ORF | 2184 bp |
| Locus ID | 5351 |
| UniProt ID | Q02809 |
| Domains | 2OG-FeII_Oxy, P4Hc |
| Protein Families | Druggable Genome |
| Protein Pathways | Lysine degradation |
| Gene Summary | Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Aberrantly expressed PLOD1 promotes cancer aggressiveness in bladder cancer: a potential prognostic marker and therapeutic target
,Yamada, Y;Kato, M;Arai, T;Sanada, H;Uchida, A;Misono, S;Sakamoto, S;Komiya, A;Ichikawa, T;Seki, N;,
Mol Oncol
,PubMed ID 31199049
[PLOD1]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201969 | PLOD1 (Myc-DDK-tagged)-Human procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1 (PLOD1) |
CNY 5,320.00 |
|
| RC201969L1 | Lenti ORF clone of Human procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1 (PLOD1), Myc-DDK-tagged |
CNY 7,728.00 |
|
| RC201969L2 | Lenti ORF clone of Human procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1 (PLOD1), mGFP tagged |
CNY 7,700.00 |
|
| RC201969L3 | Lenti ORF clone of Human procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1 (PLOD1), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC201969L4 | Lenti ORF clone of Human procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1 (PLOD1), mGFP tagged |
CNY 7,700.00 |
|
| RG201969 | PLOD1 (tGFP-tagged) - Human procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1 (PLOD1) |
CNY 6,928.00 |
