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TPMT (NM_000367) Human Untagged Clone
CAT＃: SC119934
TPMT (untagged)-Human thiopurine S-methyltransferase (TPMT)
CNY 2,400.00
CNY 2,950.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | TPMTD |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119934 sequence for NM_000367 edited (data generated by NextGen Sequencing)
ATGGATGGTACAAGAACTTCACTTGACATTGAAGAGTACTCGGATACTGAGGTACAGAAA AACCAAGTACTAACTCTGGAAGAATGGCAAGACAAGTGGGTGAACGGCAAGACTGCTTTT CATCAGGAACAAGGACATCAGCTATTAAAGAAGCATTTAGATACTTTCCTTAAAGGCAAG AGTGGACTGAGGGTATTTTTTCCTCTTTGCGGAAAAGCGGTTGAGATGAAATGGTTTGCA GACCGGGGACACAGTGTAGTTGGTGTGGAAATCAGTGAACTTGGGATACAAGAATTTTTT ACAGAGCAGAATCTTTCTTACTCAGAAGAACCAATCACCGAAATTCCTGGAACCAAAGTA TTTAAGAGTTCTTCGGGGAACATTTCATTGTACTGTTGCAGTATTTTTGATCTTCCCAGG ACAAATATTGGCAAATTTGACATGATTTGGGATAGAGGAGCATTAGTTGCCATTAATCCA GGTGATCGCAAATGCTATGCAGATACAATGTTTTCCCTCCTGGGAAAGAAGTTTCAGTAT CTCCTGTGTGTTCTTTCTTATGATCCAACTAAACATCCAGGTCCACCATTTTATGTTCCA CATGCTGAAATTGAAAGGTTGTTTGGTAAAATATGCAATATACGTTGTCTTGAGAAGGTT GATGCTTTTGAAGAACGACATAAAAGTTGGGGAATTGACTGTCTTTTTGAAAAGTTATAT CTACTTACAGAAAAGTAA Clone variation with respect to NM_000367.2 474 c=>t >OriGene 5' read for NM_000367 unedited
GTTCGGGATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGGTGGAAGCG GAGGCGTACCCGCCCCTGGGGACGTCATTGGTGGCGGAGGCAATGGCCGGCAACCAGCTG TAAGCGAGGCACGGAAGACATATGCTTGTGAGACAAAGGTGTCTCTGAAACTATGGATGG TACAAGAACTTCACTTGACATTGAAGAGTACTCGGATACTGAGGTACAGAAAAACCAAGT ACTAACTCTGGAAGAATGGCAAGACAAGTGGGTGAACGGCAAGACTGCTTTTCATCAGGA ACAAGGACATCAGCTATTAAAGAAGCATTTAGATACTTTCCTTAAAGGCAAGAGTGGACT GAGGGTATTTTTTCCTCTTTGCGGAAAAGCGGTTGAGATGAAATGGTTTGCAGACCGGGG ACACAGTGTAGTTGGTGTGGAAATCAGTGAACTTGGGATACAAGAATTTTTTACAGAGCA GAATCTTTCTTACTCAGAAGAACCAATCACCGAAATTCCTGGAACCAAAGTATTTAAGAG TTCTTCGGGGAACATTTCATTGTACTGTTGCAGTATTTTTGATCTTCCCAGGACAAATAT TGGCAAATTTGACATGATTTGNGATAGAGGAGCATTAGTTGCCATTAATCCAGGTGATCG CAAATGCTATGCAGATACAATGTTTTCCCTCCTGGGAAAGAAGTTTCAGTATCTCCTGTG TGTTCTTTCTTATGATCCAACTAAACATCCACGTCCACCATTTTATGTTCCACATGCTGA AATTGAAAGGTTGGTTGGTAAAATATGCAATATACGTTGTCCTTGAGAAAGTTGATGCCT CTTGAAGACGACATAAAAGCTGGGGAATTGACTGTCTTTTTTGAAAGTATATCTACTCCA GCAAAGTAAATGAGCN >OriGene 3' read for NM_000367 unedited
GCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTCTTTCTTTTGAGACAGAGTCTTGCTC TGTTGCCCAGGCTGGCGTGCAGTGGCATGATCTCAGCTCACTGCAACCTCCGCCTCCTAG GTTCAAGTGATTCTCCTGCCTCAGCCTCCCAAGCAGCTGGGATTACAGGCACGTGCCACC ACGCCTGGCTAATTTTTTTGTATTTTTAGTAGAGACGGGGTTGCTTTTCTATTCCCAAGT CCAAAAGCCTATGAAGAGGGCCAGCGCAATGGCTCATACCTGTAATCCTAGCACTTTGGG TGGCCAAGACAGGAGGACTGCTTGAGGCCTCACGTCAAGTTCAACATCAGCGTGGGCAAC AAAGTGAGACCCTGTCTCTACATAAAAGTAAAATCAAAAGTCTCTGGAGAGAATTGTGTT GGTCCAGCTCGGGTTAGTTGTCCATTCAACCGGTGATACAGGCAGAGTTGGGGAGTCATG GCTTCTGAAGCCCACAGTATCGACTGGGAGCAGGTCTCAGAGAAGCAAGAATCACTGTGA GCAAGGAGTTGCTTCAAATTAATAGCCTTACAAATCTATTAGAATACATCGATGGCAGAG AGCTTCTAGGGTAGAAGTTTGTACATGCAGGCCAAGGGGAACTAGAGAGGCTGANCAGAC ATATCTCTGGNGAGAGTACCGTAGCTTTCACCAGTGTTAAGGGGGACCCCAAACCTTGCA TTNAACACCTTNTAGCCAGCACATGTGCGTGCATGCGTGCACACCACACGTAACTCGTTT TTTATAATACACACCCATCCTTATAACATGAGCTAAANCATCTAAACCATTTTAGTTGTG GGTAGTACAGAGTTATAGAGGACTGTCTGATATACAAATGTTGGTAAAGAAGACTGANAA ATCTACCTTTTCATAATAACAGCCTGAATCCAGCTCTAGTACATTATATTTTATAGACCG GTGATTTAAAACGATTAATTTNANAAACAACANTNGCCNACTCAAAGAAACTTAAN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000367 |
| Insert Size | 2970 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000367.2, NP_000358.1 |
| RefSeq Size | 3258 bp |
| RefSeq ORF | 738 bp |
| Locus ID | 7172 |
| UniProt ID | P51580 |
| Protein Families | Druggable Genome |
| Protein Pathways | Drug metabolism - other enzymes |
| Gene Summary | This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals, causing thiopurine S-methyltransferase deficiency. Related pseudogenes have been identified on chromosomes 3, 18 and X. [provided by RefSeq, Aug 2014] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Pharmacogenetic variants in TPMT alter cellular responses to cisplatin in inner ear cell lines
,Bhavsar, AP;Gunaretnam, EP;Li, Y;Hasbullah, JS;Carleton, BC;Ross, CJ;,
PLoS ONE
,PubMed ID 28406961
[TPMT]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203309 | TPMT (Myc-DDK-tagged)-Human thiopurine S-methyltransferase (TPMT) |
CNY 2,400.00 |
|
| RC203309L1 | Lenti ORF clone of Human thiopurine S-methyltransferase (TPMT), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC203309L2 | Lenti ORF clone of Human thiopurine S-methyltransferase (TPMT), mGFP tagged |
CNY 5,890.00 |
|
| RC203309L3 | Lenti ORF clone of Human thiopurine S-methyltransferase (TPMT), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203309L4 | Lenti ORF clone of Human thiopurine S-methyltransferase (TPMT), mGFP tagged |
CNY 5,890.00 |
|
| RG203309 | TPMT (tGFP-tagged) - Human thiopurine S-methyltransferase (TPMT) |
CNY 4,000.00 |
