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GCLC (NM_001498) Human Untagged Clone
CAT＃: SC119206
GCLC (untagged)-Human glutamate-cysteine ligase, catalytic subunit (GCLC), transcript variant 1
CNY 4,752.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GCL; GCS; GLCL; GLCLC |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119206 sequence for NM_001498 edited (data generated by NextGen Sequencing)
ATGGGGCTGCTGTCCCAGGGCTCGCCGCTGAGCTGGGAGGAAACCAAGCGCCATGCCGAC CACGTGCGGCGGCACGGGATCCTCCAGTTCCTGCACATCTACCACGCCGTCAAGGACCGG CACAAGGACGTTCTCAAGTGGGGCGATGAGGTGGAATACATGTTGGTATCTTTTGATCAT GAAAATAAAAAAGTCCGGTTGGTCCTGTCTGGGGAGAAAGTTCTTGAAACTCTGCAAGAG AAGGGGGAAAGGACAAACCCAAACCATCCTACCCTTTGGAGACCAGAGTATGGGAGTTAC ATGATTGAAGGGACACCAGGACAGCCCTACGGAGGAACAATGTCCGAGTTCAATACAGTT GAGGCCAACATGCGAAAACGCCGGAAGGAGGCTACTTCTATATTAGAAGAAAATCAGGCT CTTTGCACAATAACTTCATTTCCCAGATTAGGCTGTCCTGGGTTCACACTGCCCGAGGTC AAACCCAACCCAGTGGAAGGAGGAGCTTCCAAGTCCCTCTTCTTTCCAGATGAAGCAATA AACAAGCACCCTCGCTTCAGTACCTTAACAAGAAATATCCGACATAGGAGAGGAGAAAAG GTTGTCATCAATGTACCAATATTTAAGGACAAGAATACACCATCTCCATTTATAGAAACA TTTACTGAGGATGATGAAGCTTCAAGGGCTTCTAAGCCGGATCATATTTACATGGATGCC ATGGGATTTGGAATGGGCAATTGCTGTCTCCAGGTGACATTCCAAGCCTGCAGTATATCT GAGGCCAGATACCTTTATGATCAGTTGGCTACTATCTGTCCAATTGTTATGGCTTTGAGT GCTGCATCTCCCTTTTACCGAGGCTATGTGTCAGACATTGATTGTCGCTGGGGAGTGATT TCTGCATCTGTAGATGATAGAACTCGGGAGGAGCGAGGACTGGAGCCATTGAAGAACAAT AACTATAGGATCAGTAAATCCCGATATGACTCAATAGACAGCTATTTATCTAAGTGTGGT GAGAAATATAATGACATCGACTTGACGATAGATAAAGAGATCTACGAACAGCTGTTGCAG GAAGGCATTGATCATCTCCTGGCCCAGCATGTTGCTCATCTCTTTATTAGAGACCCACTG ACACTGTTTGAAGAGAAAATACACCTGGATGATGCTAATGAGTCTGACCATTTTGAGAAT ATTCAGTCCACAAATTGGCAGACAATGAGATTTAAGCCCCCTCCTCCAAACTCAGACATT GGATGGAGAGTAGAATTTCGACCCATGGAGGTGCAATTAACAGACTTTGAGAACTCTGCC TATGTGGTGTTTGTGGTACTGCTCACCAGAGTGATCCTTTCCTACAAATTGGATTTTCTC ATTCCACTGTCAAAGGTTGATGAGAACATGAAGGTAGCACAGAAAAGAGATGCTGTCTTG CAGGGAATGTTTTATTTCAGGAAAGATATTTGCAAAGGTGGCAATGCAGTGGTGGATGGT TGTGGCAAGGCCCAGAACAGCACGGAGCTCGCTGCAGAGGAGTACACCCTCATGAGCATA GACACCATCATCAATGGGAAGGAAGGTGTGTTTCCTGGACTGATCCCAATTCTGAACTCT TACCTTGAAAACATGGAAGTGGATGTGGACACCAGATGTAGTATTCTGAACTACCTAAAG CTAATTAAGAAGAGAGCATCTGGAGAACTAATGACAGTTGCCAGATGGATGAGGGAGTTT ATCGCAAACCATCCTGACTACAAGCAAGACAGTGTCATAACTGATGAAATGAATTATAGC CTTATTTTGAAGTGTAACCAAATTGCAAATGAATTATGTGAATGCCCAGAGTTACTTGGA TCAGCATTTAGGAAAGTAAAATATAGTGGAAGTAAAACTGACTCATCCAACTAG Clone variation with respect to NM_001498.3 >OriGene 5' read for NM_001498 unedited
AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGCAGCCGCCTTCTGCGGGCGGCTG AGTGTCCGTCTCGCGCCCGGAGCGGGCGACCGCCGTCAGCCCGGAGGAGGAGGAGGAGGA GGAGGGGGCGGCCATGGGGCTGCTGTCCCAGGGCTCGCCGCTGAGCTGGGAGGAAACCAA GCGCCATGCCGACCACGTGCGGCGGCACGGGATCCTCCAGTTCCTGCACATCTACCACGC CGTCAAGGACCGGCACAAGGACGTTCTCAAGTGGGGCGATGAGGTGGAATACATGTTGGT ATCTTTTGATCATGAAAATAAAAAAGTCCGGTTGGTCCTGTCTGGGGAGAAAGTTCTTGA AACTCTGCAAGAGAAGGGGGAAAGGACAAACCCAAACCATCCTACCCTTTGGAGACCAGA GTATGGGAGTTACATGATTGAAGGGACACCAGGACAGCCCTACGGAGGAACAATGTCCGA GTTCAATACAGTTGAGGCCAACATGCGAAAACGCCGGAAGGAGGCTACTTCTATATTAGA AGAAAATCAGGCTCTTTGCACAATAACTTCATTTCCCAGATTAGGCTGTCCTGGGTTCAC ACTGCCCGAGGTCAAACCCAACCCAGTGGAAGGAGGAGCTTCCAAGTCCCTCTTCTTTCC AGATGAAGCAATAAACAAGCACCCTCGCTTCAGTACCTTAACAAGAATATCCGACATANG AGAGGAGAAAAGGGTGTCATCAATGTACCAATATTTAAGGACCAGAATACACCATCTNCA TTTATAGAAACATTTACTGAGGATGATGAAGCTTCAAGGGNCTCTAGCCGNATCATATNT ACATGGATGCCATGGGATNNTGAATGGGCAATNGCTGTCTNNCAGTGACATTCCAGCCTG CAGTATATCTGAGGCCGATACCTTTATGATCAGTGCTACATCTGTCCAATGTATGGNCTT GATGCTGCATCTCCTTTACGAGGCTTGTGTCAAACTGATGTCN >OriGene 3' read for NM_001498 unedited
NNGGTTTAGACTTGNACCGCGCCGCAAACTANGATCGAGTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTCAAATTCAAGGGGTATTTATTCAAAGGGGAAAGCTTGGGGCA AAGGTGACCAAGTGATTATTCCAATTGCAAAAGAAACTCATAGATAAGGGGCTAGAAACC CAAAATTACCCTTGAAGCACTTTCAATGGATTCAAGTGATTTAATGTAGACAACAAAGGA AATGACCAAGGAAAATTAACGAGAGAAAATGTTTTTAAAGAAAAAAATTTCAATAAATCA GGTCCCAGGTAGTCTTTAAAAGAACAAAAATTTACAGTAAACATTTTAACTCTGGAATGC AAGTATTGTACAATTACCAGTACATTTACAAAACTGCTTAAACAGTAGGTTGCTCCAAAG TAAGAATTTAAAAATGTACAAGCCAGTTCATGATGACTTTAGATATGTTATTAAAATACA TTGTTAATCAAAAATACTTTACTATGTACATGTACACTGTATAAACTCTAGATTTACCTA CCAAAGAAAGCCTTAATCAATTTCTGGCTCACTGGCCCAAAAAACAGGACAGTTCTATCA TTTGGGCTTCATATTATACACACGGGCTGAGAGGCATGGTACTGTACCCAGTTCGTCAAT AATGCATTTTTCTTTCTGTAAAATGTCTAGTTGGATGAGTCAGTTTTACTTCCACTATAT TTTACTTTCCTAAATGCTGATCCAAGTAACTCTGGGCATTCACATAATTCATTTGCGATT TGGTTACACTTCAAAAAAGGCTATAATTCATTTCATCAGTTATGACACTGTCTTGCTTGT AATCAGAATGGTTTGCGATAAACTCCCTCAAACATCTGGCAACTGTCATTAGTTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001498 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001498.2, NP_001489.1 |
| RefSeq Size | 3717 bp |
| RefSeq ORF | 1914 bp |
| Locus ID | 2729 |
| UniProt ID | P48506 |
| Domains | GCS |
| Protein Families | Druggable Genome |
| Protein Pathways | Glutathione metabolism, Metabolic pathways |
| Gene Summary | Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207915 | GCLC (Myc-DDK-tagged)-Human glutamate-cysteine ligase, catalytic subunit (GCLC), transcript variant 1 |
CNY 4,744.00 |
|
| RC207915L1 | Lenti ORF clone of Human glutamate-cysteine ligase, catalytic subunit (GCLC), transcript variant 1, Myc-DDK-tagged |
CNY 7,144.00 |
|
| RC207915L2 | Lenti ORF clone of Human glutamate-cysteine ligase, catalytic subunit (GCLC), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC207915L3 | Lenti ORF clone of Human glutamate-cysteine ligase, catalytic subunit (GCLC), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207915L4 | Lenti ORF clone of Human glutamate-cysteine ligase, catalytic subunit (GCLC), transcript variant 1, mGFP tagged |
CNY 7,144.00 |
|
| RG207915 | GCLC (tGFP-tagged) - Human glutamate-cysteine ligase, catalytic subunit (GCLC), transcript variant 1 |
CNY 6,344.00 |
