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MEK3 (MAP2K3) (NM_002756) Human Untagged Clone
CAT＃: SC118431
MAP2K3 (untagged)-Human mitogen-activated protein kinase kinase 3 (MAP2K3), transcript variant A
CNY 2,400.00
CNY 6,270.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MAPKK3; MEK3; MKK3; PRKMK3; SAPKK-2; SAPKK2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_002756 edited
GAATTCGGCACGAGGCCTCGTGCCGAATTCGGCACGAGGGGGCCGTCTGCCCGCAGCCAT GAGCGTGCTCGGCCCCGGTGGAGCCCGCAGTCCTCTAGATTAGTCTCCACCGCCGTCCAG GACCCACTTGCAGCATGGAGTCGCCCGCCTCGAGCCAGCCCGCCAGCATGCCCCAGTCCA AAGGAAAATCCAAGAGGAAGAAGGATCTACGGATATCCTGCATGTCCAAGCCACCCGCAC CCAACCCCACACCCCCCCGGAACCTGGACTCCCGGACCTTCATCACCATTGGAGACAGAA ACTTTGAGGTGGAGGCTGATGACTTGGTGACCATCTCAGAACTGGGCCGTGGAGCCTATG GGGTGGTAGAGAAGGTGCGGCACGCCCAGAGCGGCACCATCATGGCCGTGAAGCGGATCC GGGCCACCGTGAACTCACAGGAGCAGAAGCGGCTGCTCATGGACCTGGACATCAACATGC GCACGGTCGACTGTTTCTACACTGTCACCTTCTACGGGGCACTATTCAGAGAGGGAGACG TGTGGATCTGCATGGAGCTCATGGACACATCCTTGGACAAGTTCTACCGGAAGGTGCTGG ATAAAAACATGACAATTCCAGAGGACATCCTTGGGGAGATTGCTGTGTCTATCGTGCGGG CCCTGGAGCATCTGCACAGCAAGCTGTCGGTGATCCACAGAGATGTGAAGCCCTCCAATG TCCTTATCAACAAGGAGGGCCATGTGAAGATGTGTGACTTTGGCATCAGTGGCTACTTGG TGGACTCTGTGGCCAAGACGATGGATGCCGGCTGCAAGCCCTACATGGCCCCTGAGAGGA TCAACCCAGAGCTGAACCAGAAGGGCTACAATGTCAAGTCCGACGTCTGGAGCCTGGGCA TCACCATGATTGAGATGGCCATCCTGCGGTTCCCTTACGAGTCCTGGGGGACCCCGTTCC AGCAGCTGAAGCAGGTGGTGGAGGAGCCGTCCCCCCAGCTCCCAGCCGACCGTTTCTCCC CCGAGTTTGTGGACTTCACTGCTCAGTGCCTGAGGAAGAACCCCGCAGAGCGTATGAGCT ACCTGGAGCTGATGGAGCACCCCTTCTTCACCTTGCACAAAACCAAGAAGACGGACATTG CTGCCTTCGTGAAGGAGATCCTGGGAGAAGACTCATAGGGGCTGGGCCTCGGACCCCACT CCGGCCCTCCAGAGCCCCACAGCCCCATCTGCGGGGGCAGTGCTCACCCACACCATAAGC TACTGCCATCCTGGCCCAGGGCATCTGGGAGGAACCGAGGGGGCTGCTCCCACCTGGCTC TGTGGCGAGCCATTTGTCCCAAGTGCCAAAGAAGCAGACCATTGGGGCTCCCAGCCAGGC CCTTGTCGGCCCCACCAGTGCCTCTCCCTGCTGCTCCTAGGACCCGTCTCCAGCTGCTGA GATCCTGGACTGAGGGGGCCTGGATGCCCCCTGTGGATGCTGCTGCCCCTGCACAGCAGG CTGCCAGTGCCTGGGTGGATGGGCCACCGCCTTGCCCAGCCTGGATGCCATCCAAGTTGT ATATTTTTTTAATCTCTCGACTGAATGGACTTTGCACACTTTGGCCCAGGGTGGCCACAC CTCTATCCCGGCTTTGGTGCGGGGTACACAAGAGGGGATGAGTTGTGTGAATACCCCAAG ACTCCCATGAGGGAGATGCCATGAGCCGCCCAAGGCCTTCCCCTGGCACTGGCAAACAGG GCCTCTGCGGAGCACACTGGCTCACCCAGTCCTGCCCGCCACCGTTATCGGTGTCATTCA CCTTTCGTGTTTTTTTTAATTTATCCTCTGTTGATTTTTTCTTTTGCTTTATGGGTTTGG CTTGTTTTTCTTGCATGGTTTGGAGCTGATCGCTTCTCCCCCACCCCCTAGGGTACCAGC AGGCAGAGCCTTGCCCTCTGCTCAGGCTGGGGTCCAGTGGGAGGGGCCCAAGATCTCTGC TCAGAGAAGTGCAGGGGGAGCCTTCCAGCTCACTCTCCCTGAGGACTGGCTTGACAGGGG CTATGGGTTTGCTTTGGTGTTGTTTTTAAAAAAAGAAAATATATTTTTTTGAAAAAACGA CTGCCCATCCCGGGTCCTTTCCCTGATGGGTTGGGGCAGTTACCTGGTTGCTGTTTTAAT TAAAAAAAAAAAAAAAAAACTCGAC >OriGene 5' read for NM_002756 unedited
NGGGTTGGTAGTTGTATACGATCACTATAGGCGGCCGCGAATTCGCACGAGCCTCGTGCC GAATTCGGCACGAGGGGGCCGTCTGCCCGCAGCCATGAGCGTGCTCGGCCCCGGTGGAGC CCGCAGTCCTCTAGATTAGTCTCCACCGCCGTCCAGGACCCACTTGCAGCATGGAGTCGC CCGCCTCGAGCCAGCCCGCCAGCATGCCCCAGTCCAAAGGAAAATCCAAGAGGAAGAAGG ATCTACGGATATCCTGCATGTCCAAGCCACCCGCACCCAACCCCACACCCCCCCGGAACC TGGACTCCCGGACCTTCATCACCATTGGAGACAGAAACTTTGAGGTGGAGGCTGATGACT TGGTGACCATCTCAGAACTGGGCCGTGGAGCCTATGGGGTGGTAGAGAAGGTGCGGCACG CCCAGAGCGGCACCATCATGGCCGTGAAGCGGATCCGGGCCACCGTGAACTCACAGGAGC AGAAGCGGCTGCTCATGGACCTGGACATCAACATGCGCACGGTCGACTGTTTCTACACTG TCACCTTCTACGGGGCACTATTCANAGAGGGAGACGTGTGGATCTGCATGGAGCTCATGG ACACATCCTTGGACAAGTTCTACCGGAAGGTGCTGGATAAAAACATGACAATTCCAGAGG ACATCCTTGGGGAGATTGCTGCGTCTATCGTGCGGGCCCTGGAGCATCTGCACAGCGAGC TGTCGGTGATCCACAGAGATGTGAAGCCCTCCAATGTCCTTATCAACAAGGAGGGCCATG TGAAGAAGTGTGACTTTGGCATCAGTGGCTACCTGGTGGACTTTGTGGCCCAGACAAGGG TGCCCGCTGGCAACCCTAACTGGGCCCTGAGAGGTACAACCCAGAGCTGACCCGCAGGGC TTCCATGTN >OriGene 3' read for NM_002756 unedited
ATTTGGCCGCGGCCGCATTTTANATCGAGTTTTTTTTTTTTTTTTTTAATTAAAACAGCA ACCAGGTAACTGCCCCAACCCATCAGGGAAAGGACCCGGGATGGGCAGTCGTTTTTTCAA AAAAATATATTTTCTTTTTTTAAAAACAACACCAAAGCAAACCCATAGCCCCTGTCAAGC CAGTCCTCAGGGAGAGTGAGCTGGAAGGCTCCCCCTGCACTTCTCTGAGCAGAGATCTTG GGCCCCTCCCACTGGACCCCAGCCTGAGCAGAGGGCAAGGCTCTGCCTGCTGGTACCCTA GGGGGTGGGGGAGAAGCGATCAGCTCCAAACCATGCAAGAAAAACAAGCCAAACCCATAA AGCAAAAGAAAAAATCAACAGAGGATAAATTAAAAAAAACACGAAAGGTGAATGACACCG ATAACGGTGGCGGGCAGGACTGGGTGAGCCAGTGTGCTCCGCAGAGGCCCTGTTTGCCAG TGCCAGGGGAAGGCCTTGGGCGGCTCATGGCATCTCCCTCATGGGAGTCTTGGGGTATTC ACACAACTCATCCCCTCTTGTGTACCCCGCACCAAAGCCGGGATAGAGGTGTGGCCACCC TGNGCCAAGTGTGCANAGTCCATTCAGTCGAGAGATAAAAAAAATATACACTTGGATGGG CATCCAGCTGGGCAGGGCGGTGGCCCATCCACCCAGCACTGGCAGCCTGCTGTGCANGGG CAGCAGCATCCACAGGGGGCATNNCAGCCCNCTCAGTCCAGATCTCAGCAGCTGGAGACG GGTTCCTAGAGCANCAGGGAGAAGCACTGGTGGGGCCGACAGAGGCCTGGCTGGGAGCCC ACATGGTCTGCTTCTTTGGCACTTGGNACAAATGCCTCGCCACAGACCAGGTGGGAGCAG CCCCTCTGTTCCTCCAAATGCCCTGGCCANGATGGCAGTANCTAATGTGTGGGTGAACAC CTGCCCGCAGATGGGCTGC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002756 |
| Insert Size | 2210 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002756.2, NP_002747.2 |
| RefSeq Size | 2029 bp |
| RefSeq ORF | 957 bp |
| Locus ID | 5606 |
| UniProt ID | P46734 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase, Transcription Factors |
| Protein Pathways | Amyotrophic lateral sclerosis (ALS), Fc epsilon RI signaling pathway, GnRH signaling pathway, MAPK signaling pathway, Toll-like receptor signaling pathway |
| Gene Summary | The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (A) represents use of an alternate promoter and 5' UTR and uses a downstream start codon compared to variant B. The resulting isoform (A) is shorter at the N-terminus compared to isoform B. Variants A and D both encode the same isoform (A). |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218101 | MAP2K3 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase 3 (MAP2K3), transcript variant A |
CNY 2,400.00 |
|
| RC218101L3 | Lenti-ORF clone of MAP2K3 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase 3 (MAP2K3), transcript variant A |
CNY 5,890.00 |
|
| RC218101L4 | Lenti-ORF clone of MAP2K3 (mGFP-tagged)-Human mitogen-activated protein kinase kinase 3 (MAP2K3), transcript variant A |
CNY 5,890.00 |
|
| RG218101 | MAP2K3 (tGFP-tagged) - Human mitogen-activated protein kinase kinase 3 (MAP2K3), transcript variant A |
CNY 4,370.00 |
