United States
Germany
Japan
United Kingdom
China
GPBB (PYGB) (NM_002862) Human Untagged Clone
CAT＃: SC118380
PYGB (untagged)-Human phosphorylase, glycogen, brain (PYGB)
CNY 6,184.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GPBB |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118380 sequence for NM_002862 edited (data generated by NextGen Sequencing)
ATGGCGAAGCCGCTGACGGACAGCGAGAAGCGGAAGCAGATCAGCGTGCGCGGCCTGGCG GGGCTAGGCGACGTGGCCGAGGTGCGGAAGAGCTTCAACCGGCACTTGCACTTCACGCTG GTCAAGGACCGCAATGTGGCCACGCCCCGCGACTACTTCTTCGCGCTGGCGCACACGGTG CGCGACCACCTCGTGGGCCGCTGGATCCGCACGCAGCAGCACTACTACGAGCGCGACCCC AAGCGCATTTATTATCTTTCCCTGGAATTCTACATGGGTCGCACGCTGCAGAACACGATG GTGAACCTGGGCCTTCAGAATGCCTGCGATGAAGCCATCTATCAGTTGGGGTTAGACTTG GAGGAACTCGAGGAGATAGAAGAAGATGCTGGCCTTGGGAATGGAGGCCTGGGGAGGCTG GCAGCGTGTTTCCTTGACTCAATGGCTACCTTGGGCCTGGCAGCATACGGCTATGGAATC CGCTATGAATTTGGGATTTTTAACCAGAAGATTGTCAATGGCTGGCAGGTAGAGGAGGCC GATGACTGGCTGCGCTACGGCAACCCCTGGGAGAAAGCGCGGCCTGAGTATATGCTTCCC GTGCACTTCTACGGACGCGTGGAGCACACCCCCGACGGCGTGAAGTGGCTGGACACACAG GTGGTGCTGGCCATGCCCTACGACACCCCAGTGCCCGGCTACAAGAACAACACCGTCAAC ACCATGCGGCTGTGGTCCGCCAAGGCTCCCAACGACTTCAAGCTGCAGGACTTCAACGTG GGAGACTACATCGAGGCGGTCCTGGACCGGAACTTGGCTGAGAACATCTCCAGGGTCCTG TATCCAAATGATAACTTCTTTGAGGGGAAGGAGCTGCGGCTGAAGCAGGAGTACTTCGTG GTGGCCTCCACGCTCCAGGACATCATCCGCCGCTTCAAGTCGTCCAAGTTCGGCTGCCGG GACCCTGTGAGAACCTGTTTCGAGACGTTCCCAGACAAGGTGGCCATCCAGCTGAACGAC ACCCACCCCGCCCTCTCCATCCCTGAGCTCATGCGGATCCTGGTGGACGTGGAGAAGGTG GACTGGGACAAGGCCTGGGAAATCACGAAGAAGACCTGTGCGTACACCAACCACACTGTG CTGCCTGAGGCCTTGGAGCGCTGGCCCGTGTCCATGTTTGAGAAGCTGCTGCCGCGGCAC CTGGAGATAATCTATGCCATCAACCAGCGGCACCTGGACCACGTGGCCGCGCTGTTTCCC GGCGATGTGGACCGCCTGCGCAGGATGTCTGTGATCGAGGAGGGGGACTGCAAGCGGATC AACATGGCCCACCTGTGTGTGATTGGGTCCCATGCTGTCAATGGTGTGGCGAGGATCCAC TCGGAGATCGTGAAACAGTCGGTCTTTAAGGATTTTTATGAACTGGAGCCAGAGAAGTTC CAGAATAAGACCAATGGCATCACCCCCCGCCGGTGGCTGCTGCTGTGCAACCCGGGGCTG GCCAATACCATCGTGGAGAAAATTGGGGAGGAGTTCCTGACTGACCTGAGCCAGCTGAAG AAGCTGCTGCCGCTGGTCAGTGACGAGGTGTTCATCAGGGACGTGGCCAAGGTCAAACAG GAGAACAAGCTCAAGTTCTCGGCCTTCCTGGAGAAGGAGTACAAGGTGAAGATCAACCCC TCCTCCATGTTCGACGTGCATGTGAAGAGGATCCACGAGTACAAGCGGCAGCTGCTCAAC TGCCTGCACGTCGTCACCCTGTACAATCGAATCAAGAGAGACCCGGCCAAGGCTTTTGTG CCCAGGACTGTTATGATTGGGGGCAAGGCAGCGCCCGGTTACCACATGGCCAAGCTGATC ATCAAGTTGGTCACCTCCATCGGCGACGTCGTCAATCATGACCCAGTTGTGGGTGACAGG TTGAAAGTGATCTTCCTGGAGAACTACCGTGTGTCCTTGGCTGAGAAAGTGATCCCGGCC GCTGATCTGTCGCAGCAGATCTCCACTGCAGGCACCGAGGCCTCAGGCACAGGCAACATG AAGTTCATGCTCAACGGGGCCCTCACCATCGGCACCATGGACGGCGCCAACGTGGAGATG GCCGAGGAGGCCGGGGCCGAGAACCTCTTCATCTTCGGCCTGCGGGTGGAGGATGTCGAG GCCTTGGACCGGAAAGGGTACAATGCCAGGGAGTACTACGACCACCTGCCCGAGCTGAAG CAGGCCGTGGACCAGATCAGCAGTGGCTTTTTTTCTCCCAAGGAGCCAGACTGCTTCAAG GACATCGTGAACATGCTGATGCACCATGACAGGTTCAAGGTGTTTGCAGACTATGAAGCC TACATGCAGTGCCAGGCACAGGTGGACCAACTGTACCGGAACCCCAAGGAGTGGACCAAG AAGGTCATCAGGAACATCGCCTGCTCGGGCAAGTTCTCCAGTGACCGGACCATCACGGAG TATGCACGGGAGATCTGGGGTGTGGAGCCCTCCGACCTGCAGATCCCGCCCCCCAACATC CCCCGGGACTAG Clone variation with respect to NM_002862.3 907 g=>t;1122 a=>g;1504 g=>a;1695 t=>c;2370 g=>a >OriGene 5' read for NM_002862 unedited
AGATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGCGCCAGAGCAGCTG CACCATCCCGGCGTTCGCGTGTGCCGCCGCTTTCCTCCTCCATCTCTTTTCCTCCGCCTC CGCCGGCGCGATGGCGAAGCCGCTGACGGACAGCGAGAAGCGGAAGCAGATCAGCGTGCG CGGCCTGGCGGGGCTAGGCGACGTGGCCGAGGTGCGGAAGAGCTTCAACCGGCACTTGCA CTTCACGCTGGTCAAGGACCGCAATGTGGCCACGCCCCGCGACTACTTCTTCGCGCTGGC GCACACGGTGCGCGACCACCTCGTGGGCCGCTGGATCCGCACGCAGCAGCACTACTACGA GCGCGACCCCAAGCGCATTTATTATCTTTCCCTGGAATTCTACATGGGTCGCACGCTGCA GAACACGATGGTGAACCTGGGCCTTCAGAATGCCTGCGATGAAGCCATCTATCAGTTGGG GTTAGACTTGGAGGAACTCGAGGAGATAGAAGAAGATGCTGGCCTTGGGAATGGAGGCCT GGGGAGGCTGGCAGCGTGTTTCCTTGACTCAATGGCTACCTTGGGCCTGGCAGCATACGG CTATGGAATCCGCTATGAATTTGGGATTTTTAACCAGAAGATTGTCAATGGCTGGCAGGT AGAGGAGGCCGATGACTGGCTGCGCTACGGCAACCCCTGGGAGAAAGCGCGGCCTGAGTA TATGCTTNCCGTGCACTTCTACGGACGCGTGGAGCACACCCNCGACGGCGTGAAGTGGCT GGACACACANGTGGTGCTGGCCATGCCCTACGACACCCCAGTGCCCGGGCTACAGAACAC ACCGTCAACACATGCGGNTGTGGTCCGNNCAGGCTCCNNACGACTCAGCTGCAGACTTCA CGTGGGAACTAATCGAGGCGGTCTGGACGNNACTGCTGANACACTC >OriGene 3' read for NM_002862 unedited
NTTTTTACTCTGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTCAACACCCACAAAGGTTAAATAATTTTTTATT TAACAAGGTAAAAACAATGAGGGCCACAGGGGGGGTAAGGGGGGGGCCCCCAGGGCCCAA TTAGGGGAGTTTGGCCTCTTCCAAAAACCCGTTGCTTGGGAAAAATGTTGTAAGGGCACA ACCACAGCCAAAAATTTGCCCCACCCCATTAAAAAAAAGGTTGAAGGGTTTTGGGGGGTT GGCCACCGTGCCCAAATCCTCTTGGGGGGCATTGGAGGCCAGGAAAAAAAAAACCCTCCC CGAACCCAAAATCCAGATTGCAGGGGGTTAAATTTTTCGGAATTTCCACAGGAAAGGACA GAACCCTGTTCCTTGCCTTTTTTTTTGGCCAAGGAAAGGCAGTTTCCCACCCTTGGGAAG GGGCAAGGTTGTAAGGGGTTAACCCCCACTTCCAAGTGCCAGGCCTGTTGTCAAAAAAAA GAAAAAATGGGTTCCTCCGGAACCCCTGGTCTTTTTTCAAAACAAGGTTGTGGCATTGGT GAAAAGGGAGGAAAAATGGACCCTGCCCTTTGTGTGAACAACAGGAAATGCACCTAAGAA CACAGCAGGAGTCAGGGGGAGGGCACAAACCTGCCCCCTGCCAGGAAAAAAATGGCCCTC CTAAACCACAAAAGTGACCAAGTCCATTTTTCAGTTGTTAAAACAAGAAAAGGCTTCAAC TAATTCAATTCCCATGGGNAGTTATTTTTCTCTTTGAAAAAGCAAAACCAAGCTAAGCTG NGTTAACCAATTTAATCCTGAACAAGGCCTTTGAAAAAAAAAACCAGCCCCCACTCCAGG TCCCCCCTAATCTTCCGGGTTTCCAACA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002862 |
| Insert Size | 4370 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002862.3, NP_002853.2 |
| RefSeq Size | 4131 bp |
| RefSeq ORF | 2532 bp |
| Locus ID | 5834 |
| UniProt ID | P11216 |
| Domains | phosphorylase |
| Protein Families | Druggable Genome |
| Protein Pathways | Insulin signaling pathway, Starch and sucrose metabolism |
| Gene Summary | The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202077 | PYGB (Myc-DDK-tagged)-Human phosphorylase, glycogen, brain (PYGB) |
CNY 6,176.00 |
|
| RC202077L3 | Lenti ORF clone of Human phosphorylase, glycogen; brain (PYGB), Myc-DDK-tagged |
CNY 8,576.00 |
|
| RC202077L4 | Lenti ORF clone of Human phosphorylase, glycogen; brain (PYGB), mGFP tagged |
CNY 5,890.00 |
|
| RG202077 | PYGB (tGFP-tagged) - Human phosphorylase, glycogen; brain (PYGB) |
CNY 7,776.00 |
