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RPS27A (NM_002954) Human Untagged Clone
CAT＃: SC118296
RPS27A (untagged)-Human ribosomal protein S27a (RPS27A), transcript variant 1
CNY 1,200.00
CNY 2,950.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CEP80; HEL112; S27A; UBA80; UBC; UBCEP1; UBCEP80 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_002954 edited
GAATTCGGCACGAGGGCGGTGGAGCCGCCACCAAAATGCAGATTTTCGTGAAAACCCTTA CGGGGAAGACCATCACCCTCGAGGTTGAACCCTCGGATACGATAGAAAATGTAAAGGCCA AGATCCAGGATAAGGAAGGAATTCCTCCTGATCAGCAGAGACTGATCTTTGCTGGCAAGC AGCTGGAAGATGGACGTACTTTGTCTGACTACAATATTCAAAAGGAGTCTACTCTTCATC TTGTGTTGAGACTTCGTGGTGGTGCTAAGAAAAGGAAGAAGAAGTCTTACACCACTCCCA AGAAGAATAAGCACAAGAGAAAGAAGGTTAAGCTGGCTGTCCTGAAATATTATAAGGTGG ATGAGAATGGCAAAATTAGTCGCCTTCGTCGAGAGTGCCCTTCTGATGAATGTGGTGCTG GGGTGTTTATGGCAAGTCACTTTGACAGACATTATTGTGGCAAATGTTGTCTGACTTACT GTTTCAACAAACCAGAAGACAAGTAACTGTATGAGTTAATAAAAGACATGAACTAACAAA AAAAAAAAAAAAAAACTCGAC >OriGene 5' read for NM_002954 unedited
GTAAAGTTCATACTTGTAATCGACTCATATAGGCGGCCGCGTAATTCGCACGAGGGCGGT GGAGCCGCCCCAAATNGCAGATTTTCGTGAAACCCCTTACGGGNGAAGACCATCACCCTC GAGGTTGAACCCTCGGATACCGATAGAAAATGTAAAGGCCAAGATCCAGGATAAGGAAGG AATTCCTCCTGATCAGCAGAGACTGATCTTTGCTGGCAAGCAGCTGGAAGATGGACGTAC TTTGTCTGACTACAATATTCAAAAGGAGTCTACTCTTCATCTTGTGTTGAGACTTCGTGG TGGTGCTAAGAAAAGGAAGAAGAAGTCTTACACCACTCCCAAGAAGAATAAGCACAAGAG AAAGAAGGTTAAGCTGGCTGTCCTGAAATATTATAAGGTGGATGAGAATGGCAAAATTAG TCGCCTTCGTCGAGAGTGCCCTTCTGATGAATGTGGTGCTGGGGTGTTTATGGCAAGTCA CTTTGACAGACATTATTGTGGCAAATGTTGTCTGACTTACTGTTTCAACAAACCAGAAGA CAAGTAACTGTATGAGTTAATAAAAGACATGAACTAACAAAAAAAAAAAAAAAAAACTCG ACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTG TGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCT TGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATATATATGG GTGAGNGGGGGGGGTGTNTNNNNNACANGGGGGNNNNTTTGGNAAGGACACCGTGGGGGG CCCTGCGGGGTCTATTGGGAACCAACCTGGAGTGCATTGGCACCATCTTGGCTCACTGCC ATCTCCGCCTTCTGGGTTCCAGCCGTT >OriGene 3' read for NM_002954 unedited
AGAGTCGAGTTTTTTTTTTTTTTTTTTGTTAGTTCATGTCTTTTATTAACTCATACAGTT ACTTGTCTTCTGGTTTGTTGAAACAGTAAGTCAGACAACATTTGCCACAATAATGTCTGT CAAAGTGACTTGCCATAAACACCCCAGCACCACATTCATCAGAAGGGCACTCTCGACGAA GGCGACTAATTTTGCCATTCTCATCCACCTTATAATATTTCAGGACAGCCAGCTTAACCT TCTTTCTCTTGTGCTTATTCTTCTTGGGAGTGGTGTAAGACTTCTTCTTCCTTTTCTTAG CACCACCACGAAGTCTCAACACAAGATGAAGAGTAGACTCCTTTTGAATATTGTAGTCAG ACAAAGTACGTCCATCTTCCAGCTGCTTGCCAGCAAAGATCAGTCTCTGCTGATCAGGAG GAATTCCTTCCTTATCCTGGATCTTGGCCTTTACATTTTCTATCGTATCCGAGGGTTCAA CCTCGAGGGTGATGGTCTTCCCCGTAAGGGTTTTCACGAAAATCTGCATTTTGGTGGCGG CTCCACCGCCCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCT GACGGTTCACTAAACGAGCTCTGCTTATATAGACCCTCCACCGTACACGCCTACCGNCCA TTTGCGTCAACGGGGGCGGGGTTATTACGACATTNTGGAAAGTCCNNGTTGATTTGGTGC CAAAACAAACTCCATTGACGTCAATGGGGTGGAGACTGGNAAATNCCCGTGAGTCAAACC GCTATCCACGCCCATTGGTGTACTGGCAAAACCGCATCACATGGGTATAGCGATGACTAA TACGTANATGTACTGCCCAGTANGAAAGTNCCCGTANGGTCATGTACTGGGCATAATGCC ACGCGGCCCATTTACCGNNCATGACGTCAATGGGGGGGCGGACTNGGCATATGATCACCT TGATGTACTGCCAGTGGGCAGNNTACCGTATTACTCCCCCATGACGTNATGGGAAGTCTT ATGGNCGTCTTGGGAAACTACGTANTATGCGNCATGGGGCCGGGGGTTCTTGGCGGCA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002954 |
| Insert Size | 490 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002954.3, NP_002945.1 |
| RefSeq Size | 541 bp |
| RefSeq ORF | 471 bp |
| Locus ID | 6233 |
| UniProt ID | P62979 |
| Domains | UBQ, Ribosomal_S27 |
| Protein Families | Druggable Genome |
| Protein Pathways | Ribosome |
| Gene Summary | Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.[provided by RefSeq, Sep 2008] Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209696 | RPS27A (Myc-DDK-tagged)-Human ribosomal protein S27a (RPS27A), transcript variant 1 |
CNY 1,200.00 |
|
| RC209696L1 | Lenti ORF clone of Human ribosomal protein S27a (RPS27A), transcript variant 1, Myc-DDK-tagged |
CNY 3,600.00 |
|
| RC209696L2 | Lenti ORF clone of Human ribosomal protein S27a (RPS27A), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC209696L3 | Lenti ORF clone of Human ribosomal protein S27a (RPS27A), transcript variant 1, Myc-DDK-tagged |
CNY 3,600.00 |
|
| RC209696L4 | Lenti ORF clone of Human ribosomal protein S27a (RPS27A), transcript variant 1, mGFP tagged |
CNY 3,600.00 |
|
| RG209696 | RPS27A (tGFP-tagged) - Human ribosomal protein S27a (RPS27A), transcript variant 1 |
CNY 2,800.00 |
