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Thymine DNA glycosylase (TDG) (NM_003211) Human Untagged Clone
CAT＃: SC118135
TDG (untagged)-Human thymine-DNA glycosylase (TDG)
CNY 2,950.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | hTDG |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118135 sequence for NM_003211 edited (data generated by NextGen Sequencing)
ATGGAAGCGGAGAACGCGGGCAGCTATTCCCTTCAGCAAGCTCAAGCTTTTTATACGTTT CCATTTCAACAACTGATGGCTGAAGCTCCTAATATGGCAGTTGTGAATGAACAGCAAATG CCAGAAGAAGTTCCAGCCCCAGCTCCTGCTCAGGAACCAGTGCAAGAGGCTCCAAAAGGA AGAAAAAGAAAACCCAGAACAACAGAACCAAAACAACCAGTGGAACCCAAAAAACCTGTT GAGTCAAAAAAATCTGGCAAGTCTGCAAAATCAAAAGAAAAACAAGAAAAAATTACAGAC ACATTTAAAGTAAAAAGAAAAGTAGACCGTTTTAATGGTGTTTCAGAAGCTGAACTTCTG ACCAAGACTCTCCCCGATATTTTGACCTTCAATCTGGACATTGTCATTATTGGCATAAAC CCGGGACTAATGGCTGCTTACAAAGGGCATCATTACCCTGGACCTGGAAACCATTTTTGG AAGTGTTTGTTTATGTCAGGGCTCAGTGAGGTCCAGCTGAACCATATGGATGATCACACT CTACCAGGGAAGTATGGTATTGGATTTACCAACATGGTGGAAAGGACCACGCCCGGCAGC AAAGATCTCTCCAGTAAAGAATTTCGTGAAGGAGGACGTATTCTAGTACAGAAATTACAG AAATATCAGCCACGAATAGCAGTGTTTAATGGAAAATGTATTTATGAAATTTTTAGTAAA GAAGTTTTTGGAGTAAAGGTTAAGAACTTGGAATTTGGGCTTCAGCCCCATAAGATTCCA GACACAGAAACTCTCTGCTATGTTATGCCATCATCCAGTGCAAGATGTGCTCAGTTTCCT CGAGCCCAAGACAAAGTTCATTACTACATAAAACTGAAGGACTTAAGAGATCAGTTGAAA GGCATTGAACGAAATATGGACGTTCAAGAGGTGCAATATACATTTGACCTACAGCTTGCC CAAGAGGATGCAAAGAAGATGGCTGTTAAGGAAGAAAAATATGATCCAGGTTATGAGGCA GCATATGGTGGTGCTTACGGAGAAAATCCATGCAGCAGTGAACCTTGTGGCTTCTCTTCA AATGGGCTAATTGAGAGCGTGGAGTTAAGAGGAGAATCAGCTTTCAGTGGCATTCCTAAT GGGCAGTGGATGACCCAGTCATTTACAGACCAAATTCCTTCCTTTAGTAATCACTGTGGA ACACAAGAACAGGAAGAAGAAAGCCATGCTTAA Clone variation with respect to NM_003211.4 >OriGene 5' read for NM_003211 unedited
GTTTTGTATACGACTCATATAGGCGGCCGCGACATTCGCACGAGGGGAGCTTGAGTCCAG CCACTGTCTGGGTACTGCCAGCCATCGGGCCCAGGTCTCTGGGGTTGTCTTACCGCAGTG AGTACCACGCGGTACTACAGAGACCGGCTGCCCGTGTGCCCGGCAGGTGGAGCCGCCCGC ATCAGCGGCCTCGGGGAATGGAAGCGGAGAACGCGGGCAGCTATTCCCTTCAGCAAGCTC AAGCTTTTTATACGTTTCCATTTCAACAACTGATGGCTGAAGCTCCTAATATGGCAGTTG TGAATGAACAGCAAATGCCAGAAGAAGTTCCAGCCCCAGCTCCTGCTCAGGAACCAGTGC AAGAGGCTCCAAAAGGAAGAAAAAGAAAACCCAGAACAACAGAACCAAAACAACCAGTGG AACCCAAAAAACCTGTTGAGTCAAAAAAATCTGGCAAGTCTGCAAAATCAAAAGAAAAAC AAGAAAAAATTACAGACACATTTAAAGTAAAAAGAAAAGTAGACCGTTTTAATGGGTGTT TCAGAAGCTGAACTTCTGACCAAGACTCTCCCCGATATTTTGACCTTCAATCTGGACATT GTCATTATTGGCATAAACCCGGGACTAATGGCTGCTTACAAGGGCATCATTACCCTGGAC CTGGAACCATTTTTGGAAGTGTTTGTTTATGTCAGGGCTAANNGGAGGTNCAGCTGACCA TTGGGATGATCACACTTACAAGGAANGTATTGGTATTTGGATTTACAACATGGTGGAAAG GGACCACGCCGGCGCCAAGATCTCTCAGTAAGAATTTCTGAAGGAGGGACGTTTTTTGTT CAGAAATACAGATATCGCCGAATANCAGGTTATGGGAAATGGTTTATGAATTTTNNNNAA AAATTTTTGGGGT >OriGene 3' read for NM_003211 unedited
CCCCCCCCATTCTTGNACGNGGNCATGTATTTATCNGATTTTTTTTTTTTTTTGAGAGGG ATCTTGCTCTGTTACCCAGCTGGAGTGCAGGTGTAAATGGGGAAAATGCGNACTTTGAAA GGAATTTAAAACTTTCAATATATTTAAGAAAAGGTTCAACTGTCATGTATAGGAATAACC GCCCTGGGGCAAAAAATTTTTTATTTTTTTTATAAAAAAGGGGGGTTTACTTTCGCTGGG AGATCTCTAGGGGAGGTCCGTCATAATTTAAGGGGTATTTTCCCCATTCAAAAAAAACAA TTTTTTGTAAAGCTACAATAGTTATATACCAAAGCAATACCTAGTTACATGCTTTACACA GTCCCGGGAAAAAAAAATTTAATTGCTCCTAATCCCTGAGGCAGGGCATTTTAAAGCACC CGCACAAAAGGTCCATGTAAACAGCAGTACAGTACATTATTTAAATAACATAAAGGACTT TTACACAGTTTGACCTAGGAAAAAATAAAATCCATTATAGCCACAGTTAAAAAGCATGTT AAGATTCACAATAAGAATTTGTTTTTTTTATTATAAAGAGAAGAGCAATCATATAACCTC CTGGGGGGGGGGGAGACCTCATAAATATTTTATATTGATTGACAAAACAGCATGCTCCCT GGGTACGTATGAAATCAGAATATTCAGGAGTAACTGAGGAGGATGCAAATAACATTTTTT AGTACCCAGCTTTAATATCTACCAGACCTGGTAGTCTGGATTATTTGTAGCACCATGGAA AACCATTAGAAAATCACAAGGTATACACCTCAACCTTTCCATGAGGGTCATTTAATTTTA TGTAACAAACAGCCATGAGC |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_003211 |
| Insert Size | 3570 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003211.3, NP_003202.3 |
| RefSeq Size | 3229 bp |
| RefSeq ORF | 1233 bp |
| Locus ID | 6996 |
| UniProt ID | Q13569 |
| Domains | UDG |
| Protein Families | Druggable Genome |
| Protein Pathways | Base excision repair |
| Gene Summary | The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Base Excision Repair of Tandem Modifications in a Methylated CpG Dinucleotide
,Sassa, A;Caglayan, M;Dyrkheeva, NS;Beard, WA;Wilson, SH;,
J. Biol. Chem. April 2014
,PubMed ID 24695738
[TDG]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207113 | TDG (Myc-DDK-tagged)-Human thymine-DNA glycosylase (TDG) |
CNY 3,656.00 |
|
| RC207113L1 | Lenti ORF clone of Human thymine-DNA glycosylase (TDG), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207113L2 | Lenti ORF clone of Human thymine-DNA glycosylase (TDG), mGFP tagged |
CNY 5,890.00 |
|
| RC207113L3 | Lenti ORF clone of Human thymine-DNA glycosylase (TDG), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207113L4 | Lenti ORF clone of Human thymine-DNA glycosylase (TDG), mGFP tagged |
CNY 5,890.00 |
|
| RG207113 | TDG (tGFP-tagged) - Human thymine-DNA glycosylase (TDG) |
CNY 4,370.00 |
|
| SC324192 | TDG (untagged)-Human thymine-DNA glycosylase (TDG) |
CNY 2,950.00 |
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