Semaphorin 3F (SEMA3F) (NM_004186) Human Untagged Clone

Name: Semaphorin 3F (SEMA3F) (NM_004186) Human Untagged Clone
SKU: SC117510
Price: 5760.00

CAT＃: SC117510

SEMA3F (untagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F (SEMA3F)

Datasheet

SDS

"NM_004186" in other vectors (6)

CNY 2,950.00

货期*

现货

规格

Cited in 1 publication.

Product images

经常一起买 (4)

TA342215

Rabbit polyclonal Anti-Sema3f Antibody

CNY 5,200.00

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

CNY 4,090.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	SEMA-IV; SEMA4; SEMAK
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC117510 sequence for NM_004186 edited (data generated by NextGen Sequencing) ATGCTTGTCGCCGGTCTTCTTCTCTGGGCTTCCCTACTGACTGGGGCCTGGCCATCCTTCCCCACCCAGG ACCACCTCCCGGCCACGCCCCGGGTCCGGCTCTCATTCAAAGAGCTGAAGGCCACAGGCACCGCCCACTT CTTCAACTTCCTGCTCAACACAACCGACTACCGAATCTTGCTCAAGGACGAGGACCACGACCGCATGTAC GTGGGCAGCAAGGACTACGTGCTGTCCCTGGACCTGCACGACATCAACCGCGAGCCCCTCATTATACATT GGGCAGCCTCCCCACAGCGCATCGAGGAATGCGTGCTCTCAGGCAAGGATGTCAACGGCGAGTGTGGGAA CTTCGTCAGGCTCATCCAGCCCTGGAACCGAACACACCTGTATGTGTGCGGGACAGGTGCCTACAACCCC ATGTGCACCTATGTGAACCGCGGACGCCGCGCCCAGGCCACACCATGGACCCAGACTCAGGCGGTCAGAG GCCGCGGCAGCAGAGCCACGGATGGTGCCCTCCGCCCGATGCCCACAGCCCCACGCCAGGATTACATCTT CTACCTGGAGCCTGAGCGACTCGAGTCAGGGAAGGGCAAGTGTCCGTACGATCCCAAGCTGGACACAGCA TCGGCCCTCATCAATGAGGAGCTCTATGCTGGTGTGTACATCGATTTTATGGGCACTGATGCAGCCATCT TCCGCACACTTGGAAAGCAGACAGCCATGCGCACGGATCAGTACAACTCCCGGTGGCTGAACGACCCGTC GTTCATCCATGCTGAGCTCATTCCTGACAGTGCGGAGCGCAATGATGATAAGCTTTACTTCTTCTTCCGT GAGCGGTCGGCAGAGGCGCCGCAGAGCCCCGCGGTGTACGCCCGCATCGGGCGCATTTGCCTGAACGATG ACGGTGGTCACTGTTGCCTGGTCAACAAGTGGAGCACATTCCTGAAGGCGCGGCTCGTCTGCTCTGTCCC GGGCGAGGATGGCATTGAGACTCACTTTGATGAGCTCCAGGACGTGTTTGTCCAGCAGACCCAGGACGTG AGGAACCCTGTCATTTACGCTGTCTTTACCTCCTCTGGCTCCGTGTTCCGAGGCTCTGCCGTGTGTGTCT ACTCCATGGCTGATATTCGCATGGTCTTCAACGGGCCCTTTGCCCACAAAGAGGGGCCCAACTACCAGTG GATGCCCTTCTCAGGGAAGATGCCCTACCCACGGCCGGGCACGTGCCCTGGTGGAACCTTCACGCCATCT ATGAAGTCCACCAAGGATTATCCTGATGAGGTGATCAACTTCATGCGCAGCCACCCACTCATGTACCAGG CCGTGTACCCTCTGCAGCGGCGGCCCCTGGTAGTCCGCACAGGTGCTCCCTACCGCCTTACCACTATTGC CGTGGACCAGGTGGATGCAGCCGACGGGCGCTATGAGGTGCTTTTCCTGGGCACAGACCGCGGGACAGTG CAGAAGGTCATTGTGCTGCCCAAGGATGACCAGGAGATGGAGGAGCTCATGCTGGAGGAGGTGGAGGTCT TCAAGGATCCAGCACCCGTCAAGACCATGACCATCTCTTCTAAGAGGCAACAACTCTACGTGGCGTCAGC CGTGGGTGTCACACACCTGAGCCTGCACCGCTGCCAGGCGTATGGGGCTGCCTGTGCTGACTGCTGCCTT GCCCGGGACCCTTACTGTGCCTGGGATGGCCAGGCCTGCTCCCGCTATACAGCATCCTCCAAGAGGCGGA GCCGCCGGCAGGACGTCCGGCACGGAAACCCCATCAGGCAGTGCCGTGGGTTCAACTCCAATGCCAACAA GAATGCCGTGGAGTCTGTGCAGTATGGCGTGGCCGGCAGCGCAGCCTTCCTTGAGTGCCAGCCCCGCTCG CCCCAAGCCACTGTTAAGTGGCTGTTCCAGCGAGATCCTGGTGACCGGCGCCGAGAGATTCGTGCAGAGG ACCGCTTCCTGCGCACAGAGCAGGGCTTGTTGCTCCGTGCACTGCAGCTCAGCGATCGTGGCCTCTACTC CTGCACAGCCACTGAGAACAACTTTAAGCACGTCGTCACACGAGTGCAGCTGCATGTACTGGGCCGGGAC GCCGTCCATGCTGCCCTCTTCCCACCACTGTCCATGAGCGCCCCGCCACCCCCAGGCGCAGGCCCCCCAA CGCCTCCTTACCAGGAGTTAGCCCAGCTGCTGGCCCAGCCAGAAGTGGGCCTCATCCACCAGTACTGCCA GGGTTACTGGCGCCATGTGCCCCCCAGCCCCAGGGAGGCTCCAGGGGCACCCCGGTCTCCTGAGCCCCAG GACCAGAAAAAGCCCCGGAACCGCCGGCACCACCCTCCGGACACATGA Clone variation with respect to NM_004186.4 42:t=>c 279:t=>c 1507:a=>t 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_004186 unedited GGATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAGAGTGGAG CCTGCTTCCTGGGCCCTAGGCCCCTCCCACAATGCTTGTCGCCGGTCTTCTTCTCTGGGC TTCCCTACTGACTGGGGCCTGGCCATCCTTCCCCACCCAGGACCACCTCCCGGCCACGCC CCGGGTCCGGCTCTCATTCAAAGAGCTGAAGGCCACAGGCACCGCCCACTTCTTCAACTT CCTGCTCAACACAACCGACTACCGAATCTTGCTCAAGGACGAGGACCACGACCGCATGTA CGTGGGCAGCAAGGACTACGTGCTGTCCCTGGACCTGCACGACATCAACCGCGAGCCCCT CATTATACATTGGGCAGCCTCCCCACAGCGCATCGAGGAATGCGTGCTCTCAGGCAAGGA TGTCAACGGCGAGTGTGGGAACTTCGTCAGGCTCATCCAGCCCTGGAACCGAACACACCT GTATGTGTGCGGGACAGGTGCCTACAACCCCATGTGCACCTATGTGAACCGCGGACGCCG CGCCCAGGCCACACCATGGACCCAGACTCAGGCGGTCAGAGGCCGCGGCAGCAGAGCCAC GGATGGTGCCCTCCGCCCGATGCCCACAGCCCCACGCCAGGATTACATCTTCTACCTGGA GCCTGAGCGACTCGAGTCAGGGAAGGGCAAGTGTCCGTACGATCCCAAGCTGGACACAGC ATCGGCCCTCATCAATGAGGAGCTCTATGCTGGTGTGTACATCGATTTTATGGGCACTGA TGCAGCCATCTTCCGCACACTTGGGAAGCAGACAGCCATGCGCACGGATCAGTACAACTN CCCGGTGGGCTGACGACCCCGTCGTTCATCCATGCCTGAGCTCATTNCCTGACAGTGCGG GAGCGCAATGATGATAAGCTTTACTTCTCCTTCCGTGAGCGGTCCGCAAAGGCGCGCAAA GCCCCGCGNTGTCGCCC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_004186 unedited TTGGACGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTAAGTTTTGGAGGAT GCTGCCACTGAGGCAGGCTATATTCAACCCACCAATAAATACTGTCTTTGCAGGGGCAGG GTGTGTATAGAATATAGATATTTTATATATATATATATATATATATATATATATCTTTTA TATTAAAAGGGACAAGGGCTAGGCTGGCCCATGGCAGGCACAGGCAGCTGGCCTCATGTG TCCGGAGGGTGGTGCCGGCGGTTCCGGGGCTTTTTCTGGTCCTGGGGCTCAGGAGACCGG GGTGCCCCTGGAGCCTCCCTGGGGCTGGGGGGCACATGGCGCCAGTAACCCTGGCAGTAC TGGTGGATGAGGCCCACTTCTGGCTGGGCCAGCAGCTGGGCTAACTCCTGGTAAGGAGGC GTTGGGGGGCCTGCGCCTGGGGGTGGCGGGGCGCTCATGGACAGTGGTGGGAAGAGGGCA GCATGGACGGCGTCCCGGCCCAGTACATGCAGCTGCACTCGTGTGACGACGTGCTTAAAG TTGTTCTCAGTGGCTGTGCAGGAGTAGAGGCCACGATCGCTGAGCTGCAGTGCACGGAGC AACAAGCCCTGCTCTGTGCGCAGGAAGCGGTCCTCTGCACGAATCTCTCGGCGCCGGTCA CCAGGATCTCGCTGGAACAGCCACTTAACAGTGGCTTGGGGCGAGCGGNGCTGGCACTCA AGGAAGCTGCGCTNGCCGCCACGCCATACTGCACAGACTNCACGGCATTCTTGTTGGCAT TNGGAGTGAACCCCACGCACTGCCTGATGGGTTTTCGTGCCGGAGTNCTGNCGCNNNGCT CGCTCTGNAGGAGCTGATAGNGGAGCAGCCTGCCATCCCAGCCAGTAGGGTCCCGGGCAG CANAGTCACACAGCAGCCCCTACNCTGCNANGTGCAGCTCAGTGTTGACACCCGCTGAC
Restriction Sites	NotI-NotI
ACCN	NM_004186
Insert Size	2680 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_004186.2, NP_004177.2
RefSeq Size	3535 bp
RefSeq ORF	2358 bp
Locus ID	6405
UniProt ID	Q13275
Domains	Sema, IG, PSI
Protein Families	Secreted Protein
Protein Pathways	Axon guidance
Gene Summary	This gene encodes a member of the semaphorin III family of secreted signaling proteins that are involved in axon guidance during neuronal development. The encoded protein contains an N-terminal Sema domain, an immunoglobulin loop and a C-terminal basic domain. This gene is expressed by the endothelial cells where it was found to act in an autocrine fashion to induce apoptosis, inhibit cell proliferation and survival, and function as an anti-tumorigenic agent. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016] Transcript Variant: This variant (1) encodes the longest isoform (1).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

The use of this cDNA Clones has been cited in the following citations:
Neuropilin-2 interacts with VEGFR-2 and VEGFR-3 and promotes human endothelial cell survival and migration ,Benoit Favier, Antoine Alam, Pauline Barron, Jacques Bonnin, Patricia Laboudie, Pierre Fons, Marie Mandron, Jean-Pascal Herault, Gera Neufeld, Pierre Savi, Jean-Marc Herbert, and Françoise Bono, Blood, Aug 2006; 108: 1243-1250. [SEMA3F]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC208333	SEMA3F (Myc-DDK-tagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F (SEMA3F)	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,990.00
RC208333L1	Lenti-ORF clone of SEMA3F (Myc-DDK-tagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F (SEMA3F)	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC208333L2	Lenti-ORF clone of SEMA3F (mGFP-tagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F (SEMA3F)	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC208333L3	Lenti-ORF clone of SEMA3F (Myc-DDK-tagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F (SEMA3F)	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC208333L4	Lenti-ORF clone of SEMA3F (mGFP-tagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F (SEMA3F)	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RG208333	SEMA3F (tGFP-tagged) - Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F (SEMA3F)	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,370.00

其它Semaphorin 3F产品

Expression Plasmids

Lentiviral Particles

CRISPR

RNAi

Proteins

Antibodies