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PFKFB3 (NM_004566) Human Untagged Clone
CAT＃: SC117283
PFKFB3 (untagged)-Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 1
CNY 2,950.00
| Cited in 3 publications. |
Product images
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | iPFK-2; IPFK2; PFK2 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117283 sequence for NM_004566 edited (data generated by NextGen Sequencing)
ATGCCGTTGGAACTGACGCAGAGCCGAGTGCAGAAGATCTGGGTGCCCGTGGACCACAGG CCCTCGTTGCCCAGATCCTGTGGGCCAAAGCTGACCAACTCCCCCACCGTCATCGTCATG GTGGGCCTCCCCGCCCGGGGCAAGACCTACATCTCCAAGAAGCTGACTCGCTACCTCAAC TGGATTGGCGTCCCCACAAAAGTGTTCAACGTCGGGGAGTATCGCCGGGAGGCTGTGAAG CAGTACAGCTCCTACAACTTCTTCCGCCCCGACAATGAGGAAGCCATGAAAGTCCGGAAG CAATGTGCCTTAGCTGCCTTGAGAGATGTCAAAAGCTACCTGGCGAAAGAAGGGGGACAA ATTGCGGTTTTCGATGCCACCAATACTACTAGAGAGAGGAGACACATGATCCTTCATTTT GCCAAAGAAAATGACTTTAAGGCGTTTTTCATCGAGTCGGTGTGCGACGACCCTACAGTT GTGGCCTCCAATATCATGGAAGTTAAAATCTCCAGCCCGGATTACAAAGACTGCAACTCG GCAGAAGCCATGGACGACTTCATGAAGAGGATCAGTTGCTATGAAGCCAGCTACCAGCCC CTCGACCCCGACAAATGCGACAGGGACTTGTCGCTGATCAAGGTGATTGACGTGGGCCGG AGGTTCCTGGTGAACCGGGTGCAGGACCACATCCAGAGCCGCATCGTGTACTACCTGATG AACATCCACGTGCAGCCGCGTACCATCTACCTGTGCCGGCACGGCGAGAACGAGCACAAC CTCCAGGGCCGCATCGGGGGCGACTCAGGCCTGTCCAGCCGGGGCAAGAAGTTTGCCAGT GCTCTGAGCAAGTTCGTGGAGGAGCAGAACCTGAAGGACCTGCGCGTGTGGACCAGCCAG CTGAAGAGCACCATCCAGACGGCCGAGGCGCTGCGGCTGCCCTACGAGCAGTGGAAGGCG CTCAATGAGATCGACGCGGGCGTCTGTGAGGAGCTGACCTACGAGGAGATCAGGGACACC TACCCTGAGGAGTATGCGCTGCGGGAGCAGGACAAGTACTATTACCGCTACCCCACCGGG GAGTCCTACCAGGACCTGGTCCAGCGCTTGGAGCCAGTGATCATGGAGCTGGAGCGGCAG GAGAATGTGCTGGTCATCTGCCACCAGGCCGTCCTGCGCTGCCTGCTTGCCTACTTCCTG GATAAGAGTGCAGAGGAGATGCCCTACCTGAAATGCCCTCTTCACACCGTCCTGAAACTG ACGCCTGTCGCTTATGGCTGCCGTGTGGAATCCATCTACCTGAACGTGGAGTCCGTCTGC ACACACCGGGAGAGGTCAGAGGATGCAAAGAAGGGACCTAACCCGCTCATGAGACGCAAT AGTGTCACCCCGCTAGCCAGCCCCGAACCCACCAAAAAGCCTCGCATCAACAGCTTTGAG GAGCATGTGGCCTCCACCTCGGCCGCCCTGCCCAGCTGCCTGCCCCCGGAGGTGCCCACG CAGCTGCCTGGACAAAACATGAAAGGCTCCCGGAGCAGCGCTGACTCCTCCAGGAAACAC TGA Clone variation with respect to NM_004566.3 >OriGene 5' read for NM_004566 unedited
NAAGGGTCAGATATTTGTATACGACTCACTATAGGCGGCCGCGAAATTCGCACCAGCCGC TCTCCTGCCAGCGTCGGGATCTCGGCCCCGGGAGGCGGGCCGTCGGGCGCAGCCGCGAAG ATGCCGTTGGAACTGACGCAGAGCCGAGTGCAGAAGATCTGGGTGCCCGTGGACCACAGG CCCTCGTTGCCCAGATCCTGTGGGCCAAAGCTGACCAACTCCCCCACCGTCATCGTCATG GTGGGCCTCCCCGCCCGGGGCAAGACCTACATCTCCAAGAAGCTGACTCGCTACCTCAAC TGGATTGGCGTCCCCACAAAAGTGTTCAACGTCGGGGAGTATCGCCGGGAGGCTGTGAAG CAGTACAGCTCCTACAACTTCTTCCGCCCCGACAATGAGGAAGCCATGAAAGTCCGGAAG CAATGTGCCTTAGCTGCCTTGAGAGATGTCAAAAGCTACCTGGCGAAAGAAGGGGGACAA ATTGCGGTTTTCGATGCCACCAATACTACTAGAGAGAGGAGACACATGATCCTTCATTTT GCCAAAGAAAATGACTTTAAGGCGTTTTTCATCGAGTCGGTGTGCGACGACCCTACAGTT GTGGCCTCCAATATCATGGAAGTTAAAATCTCCAGCCCGGATTACAAAGACTGCAACTCG GCAGAAGCCATGGACGACTTCATGAAGAGGATCAGTTGCTATGAAGCCAGCTACCAGCCC CTCGACCCCGACAAATGCGACAGGGACTTGTCGCTGATCAAGGTGATTGACGTGGGCCGG AGGCTCCTGGTGAACCGGGTGCAGGACCACATCCAGAGCCGCATCGTGTACTACCTGATG AAATNCAGTGCAGCCGCGTCCATCTACCTGTGCCGGCACGGCGAGAACGAGCACACCTTC CAGGCCGATCG >OriGene 3' read for NM_004566 unedited
NNNNNNNTTTGGTACATCTAAGNACCGCGGNCCGCAANCTAGNGATCGGTTTTTTTTTTT TTTTTTTTTTGTATTTCAACCAGGTATTTTATTGGTCTGGCAACTGCAAAATATACAAAT TTCTGAAAGGCATCTCCTGTTTGAAAGCTAGCATAGCTTTATATCCCAGGTCATTAATTC AGACAAATACACAGAACACAGAGAAGTCATAAGTCACACATAGGAGGCTCCCAAACCAAT TAACAATGAAACCCAATCTCTACAGCTTTCTATTGTTTAGGCTCCTTATACATATTAAAT AAATAAATAGCACATCTGCTATCAAAATAATAAAAAAATAAATTTCAAGTATTACAAAGA AAAATATCATTGGTGTGGTTCCAAACAGTTTTTCTTCTCTCTCAAAATGAAGAGCTTTGC CCGTGGTCTCAGGACAGAATAAGGAGGGGGGAAACTCACCCAAAAACAAATAGGCAAAGT TAAAAATATTGGTCCCCGTTATTTTGTGGGGTGGAGAGTTGGAGGAATCAGTAGACGCCA AAAGTTCATGACTGTAGCTCAAACCCAACCCCCGCCCCCGGCCCCCGTCCAATGTCCCCT CAAAAGGCAGGTCACTGGCTTGCAAAGTAGGCAGTGTTCTCCTTTCTATAGTATCTCCCT CCCTGTCCAGATTCATGTTACCCTCTCTCCTATCTAGGCCCTTCGCAAACTAAAAGTCCC GTGTTAGACCAAGGGCTTCTGTATTTAGGTATTGATTTTAGGTAGTCAGAGGGGGAGAGG GACCACAGCTATCAAGCCCCTATCTGTATCAAACTTCAGAGTTGATGTTCAAAGAATGAN GCCTGCGAGGGGGACACGAGNAACTATTCTCAAGAGCAAGCACN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004566 |
| Insert Size | 4260 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004566.2, NP_004557.1 |
| RefSeq Size | 4497 bp |
| RefSeq ORF | 1563 bp |
| Locus ID | 5209 |
| UniProt ID | Q16875 |
| Domains | PGAM, 6PF2K |
| Protein Families | Druggable Genome |
| Protein Pathways | Fructose and mannose metabolism |
| Gene Summary | The protein encoded by this gene belongs to a family of bifunctional proteins that are involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate (F2,6BP), and a fructose-2,6-biphosphatase activity that catalyzes the degradation of F2,6BP. This protein is required for cell cycle progression and prevention of apoptosis. It functions as a regulator of cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and survival in tumor cells. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2016] Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A high-throughput screening campaign against PFKFB3 identified potential inhibitors with novel scaffolds.
,null,
Acta pharmacologica Sinica
,PubMed ID 36114272
[PFKFB3]
|
|
Four key steps control glycolytic flux in mammalian cells
,null,
Cell systems
,PubMed ID 29960885
[PFKFB3]
|
|
Metabolic control of the cell cycle
,null,
Cell Cycle
,PubMed ID 26431254
[PFKFB3]
|
Documents
| Product Manuals |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206043 | PFKFB3 (Myc-DDK-tagged)-Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 1 |
CNY 3,872.00 |
|
| RC206043L1 | Lenti ORF clone of Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 1, Myc-DDK-tagged |
CNY 6,272.00 |
|
| RC206043L2 | Lenti ORF clone of Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 1, mGFP tagged |
CNY 6,272.00 |
|
| RC206043L3 | Lenti ORF clone of Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC206043L4 | Lenti ORF clone of Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RG206043 | PFKFB3 (tGFP-tagged) - Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 1 |
CNY 5,040.00 |
