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EWSR1 (NM_005243) Human Untagged Clone
CAT＃: SC116868
EWSR1 (untagged)-Human Ewing sarcoma breakpoint region 1 (EWSR1), transcript variant 2
CNY 2,950.00
Product images
CNY 1,999.00
CNY 3,490.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | bK984G1.4; EWS; EWS-FLI1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_005243, the custom clone sequence may differ by one or more nucleotides
ATGGCGTCCACGGATTACAGTACCTATAGCCAAGCTGCAGCGCAGCAGGGCTACAGTGCTTACACCGCCC AGCCCACTCAAGGATATGCACAGACCACCCAGGCATATGGGCAACAAAGCTATGGAACCTATGGACAGCC CACTGATGTCAGCTATACCCAGGCTCAGACCACTGCAACCTATGGGCAGACCGCCTATGCAACTTCTTAT GGACAGCCTCCCACTGGTTATACTACTCCAACTGCCCCCCAGGCATACAGCCAGCCTGTCCAGGGGTATG GCACTGGTGCTTATGATACCACCACTGCTACAGTCACCACCACCCAGGCCTCCTATGCAGCTCAGTCTGC ATATGGCACTCAGCCTGCTTATCCAGCCTATGGGCAGCAGCCAGCAGCCACTGCACCTACAAGACCGCAG GATGGAAACAAGCCCACTGAGACTAGTCAACCTCAATCTAGCACAGGGGGTTACAACCAGCCCAGCCTAG GATATGGACAGAGTAACTACAGTTATCCCCAGGTACCTGGGAGCTACCCCATGCAGCCAGTCACTGCACC TCCATCCTACCCTCCTACCAGCTATTCCTCTACACAGCCGACTAGTTATGATCAGAGCAGTTACTCTCAG CAGAACACCTATGGGCAACCGAGCAGCTATGGACAGCAGAGTAGCTATGGTCAACAAAGCAGCTATGGGC AGCAGCCTCCCACTAGTTACCCACCCCAAACTGGATCCTACAGCCAAGCTCCAAGTCAATATAGCCAACA GAGCAGCAGCTACGGGCAGCAGAGTTCATTCCGACAGGACCACCCCAGTAGCATGGGTGTTTATGGGCAG GAGTCTGGAGGATTTTCCGGACCAGGAGAGAACCGGAGCATGAGTGGCCCTGATAACCGGGGCAGGGGAA GAGGGGGATTTGATCGTGGAGGCATGAGCAGAGGTGGGCGGGGAGGAGGACGCGGTGGAATGGGCAGCGC TGGAGAGCGAGGTGGCTTCAATAAGCCTGGTGGACCCATGGATGAAGGACCAGATCTTGATCTAGGCCCA CCTGTAGATCCAGATGAAGACTCTGACAACAGTGCAATTTATGTACAAGGATTAAATGACAGTGTGACTC TAGATGATCTGGCAGACTTCTTTAAGCAGTGTGGGGTTGTTAAGATGAACAAGAGAACTGGGCAACCCAT GATCCACATCTACCTGGACAAGGAAACAGGAAAGCCCAAAGGCGATGCCACAGTGTCCTATGAAGACCCA CCCACTGCCAAGGCTGCCGTGGAATGGTTTGATGGGAAAGATTTTCAAGGGAGCAAACTTAAAGTCTCCC TTGCTCGGAAGAAGCCTCCAATGAACAGTATGCGGGGTGGTCTGCCACCCCGTGAGGGCAGAGGCATGCC ACCACCACTCCGTGGAGGTCCAGGAGGCCCAGGAGGTCCTGGGGGACCCATGGGTCGCATGGGAGGCCGT GGAGGAGATAGAGGAGGCTTCCCTCCAAGAGGACCCCGGGGTTCCCGAGGGAACCCCTCTGGAGGAGGAA ACGTCCAGCACCGAGCTGGAGACTGGCAGTGTCCCAATCCGGGTTGTGGAAACCAGAACTTCGCCTGGAG AACAGAGTGCAACCAGTGTAAGGCCCCAAAGCCTGAAGGCTTCCTCCCGCCACCCTTTCCGCCCCCGGGT GGTGATCGTGGCAGAGGTGGCCCTGGTGGCATGCGGGGAGGAAGAGGTGGCCTCATGGATCGTGGTGGTC CCGGTGGAATGTTCAGAGGTGGCCGTGGTGGAGACAGAGGTGGCTTCCGTGGTGGCCGGGGCATGGACCG AGGTGGCTTTGGTGGAGGAAGACGAGGTGGCCCTGGGGGGCCCCCTGGACCTTTGATGGAACAGATGGGA GGAAGAAGAGGAGGACGTGGAGGACCTGGAAAAATGGATAAAGGCGAGCACCGTCAGGAGCGCAGAGATC GGCCCTACTAG >OriGene 5' read for NM_005243 unedited
AGTCAGCATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGAAGGAGA GAAAATGGCGTCCACGGATTACAGTACCTATAGCCAAGCTGCAGCGCAGCAGGGCTACAG TGCTTACACCGCCCAGCCCACTCAAGGATATGCACAGACCACCCAGGCATATGGGCAACA AAGCTATGGAACCTATGGACAGCCCACTGATGTCAGCTATACCCAGGCTCAGACCACTGC AACCTATGGGCAGACCGCCTATGCAACTTCTTATGGACAGCCTCCCACTGGTTATACTAC TCCAACTGCCCCCCAGGCATACAGCCAGCCTGTCCAGGGGTATGGCACTGGTGCTTATGA TACCACCACTGCTACAGTCACCACCACCCAGGCCTCCTATGCAGCTCAGTCTGCATATGG CACTCAGCCTGCTTATCCAGCCTATGGGCAGCAGCCAGCAGCCACTGCACCTACAAGACC GCAGGATGGAAACAAGCCCACTGAGACTAGTCAACCTCAATCTAGCACAGGGGGTTACAA CCAGCCCAGCCTAGGATATGGACAGAGTAACTACAGTTATCCCCAGGTACCTGGGAGCTA CCCCATGCAGCCAGTCACTGCACCTCCATCCTACCCTCCTACCAGCTATTCCTCTACACA GCCGACTAGTTATGATCAGAGCAGTTACTCTCAGCAGAACACCTATGGGCAACCGAGCAG CTATGGACAGCAGAGTAGCTATGGTCAACAAAGCAGCTATGGGCAGCAGCCTTCCACTAG NTACCCACCCCCANACTGGATCCTACAGCCAAGCTNCAAGTCAATATAGCCCACAGAGCA GCAGCTACGGGCAGCAGAAGTCATCCCGACAGGACCACCCCATAGCATGGGTGTTTATGG GCAGGC >OriGene 3' read for NM_005243 unedited
CGGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTAACATTTGGAATTT TATTTAAAAAAAAAAAAAAAACATCACAACCATGGAACATTGTTACAGTTAAGAAGGCCC TCTTGGGTTCTCCACAATGATACTGGAGCATGCTCACAAGGGGCTTCCCATTGTTAAAGT CTTAAACAACCATTTTTAAAAGAAGGAAGAAAAAAAAACTCCGCACACTACCATTTAACT TGTTTTAATGTTTCTTCACAAATGGTGAAAAATACTAAAGTACAGACAAGGAATAATCAT AATGTTGGGGCCAACATTATAAATATGGAATTATAAATTTAAAACATTTTCTGGTTTAAA AAATAAATCTGGTAGTCAATGCAGCTCTGCGGGGTCTCTGCATCTAGTAGGGCCGATCTC TGCGCTCCTGACGGTGCTCGCCTTTATCCATTTTTCCAGGTCCTCCACGTCCTCCTCTTC TTCCTCCCATCTGTTCCATCAAAGGTCCAGGGGGCCCCCCAGGGCCACCTCGTCTTCCTC CACCAAAGCCACCTCGGTCCATGCCCCGGCCACCACGGAAGCCACCTCTGTCTCCACCAC GGCCACCTCTGAACATTCCACCGGGACCACCACGATCCATGAGGCCACCTCTTCCTCCCC GCATGCCACCAAGGCCACCTCTGCCACGATCACCACCCGGGGGCGGAAAGGTTGGCCGAA AGAAACCTTCAGCCTTTGGGGCCTTACACTGGTTGCACTCTGGTTCTCCAGCGAAGTTTT GGTTTCCACACCCCGTATTGGGACACTGCAGTCTCCAACTCGGGCTGGACGTTTCTTCCC AAAGGGTTCCCTCGGAACCCGGGGTCCTTTGAAGGGAACCTCCTTATTTCTTCAGCCTTC TTGCACCAGGTTCCCAAGACTCCGGGCTCCTGACTCCCGAAGGGTGGGCTGCCCTGCCTA CGGGGGCAAACCCCCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005243 |
| Insert Size | 2330 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005243.1, NP_005234.1 |
| RefSeq Size | 2679 bp |
| RefSeq ORF | 2679 bp |
| Locus ID | 2130 |
| UniProt ID | Q01844 |
| Domains | RRM, zf-RanBP |
| Protein Families | Druggable Genome, Stem cell - Pluripotency, Transcription Factors |
| Gene Summary | This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14. [provided by RefSeq, Jul 2009] Transcript Variant: This variant (2) lacks an alternate in-frame exon and uses an alternate in-frame splice site in the coding region, compared to variant 1. The resulting isoform (2) is shorter than isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203709 | EWSR1 (Myc-DDK-tagged)-Human Ewing sarcoma breakpoint region 1 (EWSR1), transcript variant 2 |
CNY 4,560.00 |
|
| RC203709L1 | Lenti ORF clone of Human Ewing sarcoma breakpoint region 1 (EWSR1), transcript variant 2, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC203709L2 | Lenti ORF clone of Human Ewing sarcoma breakpoint region 1 (EWSR1), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
| RC203709L3 | Lenti ORF clone of Human Ewing sarcoma breakpoint region 1 (EWSR1), transcript variant 2, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC203709L4 | Lenti ORF clone of Human Ewing sarcoma breakpoint region 1 (EWSR1), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
| RG203709 | EWSR1 (tGFP-tagged) - Human Ewing sarcoma breakpoint region 1 (EWSR1), transcript variant 2 |
CNY 5,040.00 |
|
| SC322277 | EWSR1 (untagged)-Human Ewing sarcoma breakpoint region 1 (EWSR1), transcript variant 2 |
CNY 4,896.00 |
