EOMES (NM_005442) Human Untagged Clone

CAT#: SC116738

EOMES (untagged)-Human eomesodermin (EOMES)



  "NM_005442" in other vectors (6)

CNY 7,544.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal anti-EOMES antibody
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms TBR2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_005442 edited
ATGCAGTTAGGGGAGCAGCTCTTGGTGAGCTCAGTGAACCTGCCTGGCGCGCACTTCTAC
CCGCTGGAGAGTGCGCGAGGCGGCAGCGGCGGGAGCGCTGGCCACCTCCCCAGCGCGGCC
CCCTCTCCTCAGAAGTTGGACTTAGACAAAGCGTCCAAGAAGTTTTCCGGCAGTCTCTCC
TGCGAGGCGGTGAGCGGGGAGCCCGCAGCCGCCAGCGCAGGGGCCCCCGCGGCCATGCTT
AGTGACACCGACGCCGGGGACGCATTTGCCAGCGCTGCGGCAGTGGCCAAGCCGGGGCCC
CCGGACGGCCGCAAGGGCTCCCCCTGCGGGGAGGAGGAGCTGCCCTCCGCCGCTGCAGCC
GCCGCCGCCGCCGCCGCCGCGGCTGCGGCCACTGCGCGCTACTCCATGGACAGCCTGAGC
TCCGAGCGGTACTACCTCCAGTCCCCCGGTCCTCAGGGGTCGGAGCTGGCTGCGCCCTGC
TCACTCTTCCCGTACCAGGCGGCGGCTGGGGCGCCCCACGGACCTGTGTACCCGGCTCCT
AACGGGGCGCGCTACCCCTACGGCTCCATGCTGCCCCCCGGCGGCTTCCCCGCGGCTGTG
TGCCCACCCGGGAGGGCGCAGTTCGGCCCAGGAGCCGGTGCGGGCAGTGGCGCGGGCGGT
AGCAGCGGCGGGGGCGGCGGCCCGGGCACCTATCAGTACAGCCAGGGGGCTCCGCTCTAC
GGGCCGTACCCTGGAGCCGCAGCGGCGGGATCTTGCGGAGGACTGGGGGGCCTGGGGGTT
CCAGGTTCTGGCTTCCGTGCCCACGTCTACCTGTGCAACCGGCCTCTGTGGCTCAAATTC
CACCGCCACCAAACTGAGATGATCATTACGAAACAGGGCAGGCGCATGTTTCCTTTCTTG
AGCTTCAACATAAACGGACTCAATCCCACTGCCCACTACAATGTGTTCGTAGAGGTGGTG
CTGGCGGACCCCAACCACTGGCGCTTCCAGGGGGGCAAATGGGTGACCTGTGGCAAAGCC
GACAATAACATGCAGGGCAACAAAATGTATGTTCACCCAGAGTCTCCTAATACTGGTTCC
CACTGGATGAGACAGGAGATTTCATTCGGGAAATTAAAACTCACCAATAACAAAGGCGCA
AATAACAACAACACCCAGATGATAGTCTTACAATCCTTACACAAATACCAACCCCGACTG
CATATTGTTGAAGTTACAGAGGATGGCGTGGAGGACTTGAATGAGCCCTCAAAGACCCAG
ACTTTTACCTTCTCAGAAACGCAATTCATTGCAGTGACTGCCTACCAAAACACCGATATT
ACTCAACTAAAGATTGATCATAACCCCTTTGCAAAAGGCTTCAGAGACAACTATGATTCA
TCCCATCAGATTGTCCCTGGAGGTCGGTACGGCGTTCAATCCTTCTTCCCGGAGCCCTTT
GTCAACACTTTACCTCAAGCCCGCTATTATAATGGCGAGAGAACCGTGCCACAGACCAAC
GGCCTCCTTTCACCCCAACAGAGCGAAGAGGTGGCCAACCCTCCCCAGCGGTGGCTTGTC
ACGCCTGTCCAGCAACCTGGGACCAACAAACTAGACATCAGTTCCTATGAATCTGAATAT
ACTTCTAGCACATTGCTCCCATATGGCATTAAATCCTTGCCCCTTCAGACATCCCATGCC
CTGGGGTATTACCCAGACCCAACCTTTCCTGCAATGGCAGGGTGGGGAGGTCGAGGTTCT
TACCAGAGGAAGATGGCAGCTGGACTACCATGGACCTCCAGAACAAGCCCCACTGTGTTC
TCTGAAGATCAGCTCTCCAAGGAGAAAGTGAAAGAGGAAATTGGCTCTTCTTGGATAGAG
ACACCCCCTTCCATCAAATCTCTAGATTCCAATGATTCAGGAGTATACACCAGTGCTTGT
AAGCGAAGGCGGCTGTCTCCTAGCAACTCCAGTAATGAAAATTCACCCTCCATAAAGTGT
GAGGACATTAATGCTGAAGAGTATAGTAAAGACACCTCAAAAGGCATGGGAGGGTATTAT
GCTTTTTACACAACTCCCTAA
Restriction Sites NotI-NotI     
ACCN NM_005442
Insert Size 2100 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005442.2, NP_005433.2
RefSeq Size 2756 bp
RefSeq ORF 2061 bp
Locus ID 8320
UniProt ID O95936
Domains T-box
Protein Families Embryonic stem cells, ES Cell Differentiation/IPS, Transcription Factors
Gene Summary This gene belongs to the TBR1 (T-box brain protein 1) sub-family of T-box genes that share the common DNA-binding T-box domain. The encoded protein is a transcription factor which is crucial for embryonic development of mesoderm and the central nervous system in vertebrates. The protein may also be necessary for the differentiation of effector CD8+ T cells which are involved in defense against viral infections. A similar gene disrupted in mice is shown to be essential during trophoblast development and gastrulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]
Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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